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Pfkm phosphofructokinase, muscle [ Rattus norvegicus (Norway rat) ]

Gene ID: 65152, updated on 13-Apr-2024

Summary

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Official Symbol
Pfkmprovided by RGD
Official Full Name
phosphofructokinase, muscleprovided by RGD
Primary source
RGD:68419
See related
Ensembl:ENSRNOG00000057988 AllianceGenome:RGD:68419
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
PFK-A; Pfk-M; ATP-PFK
Summary
Enables several functions, including 6-phosphofructokinase activity; anion binding activity; and identical protein binding activity. Involved in fructose 1,6-bisphosphate metabolic process; fructose 6-phosphate metabolic process; and glycolytic process. Predicted to be located in several cellular components, including apical plasma membrane; cytosol; and sperm principal piece. Predicted to be part of 6-phosphofructokinase complex. Predicted to be active in membrane. Human ortholog(s) of this gene implicated in glycogen storage disease VII. Orthologous to human PFKM (phosphofructokinase, muscle). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Muscle (RPKM 4937.7), Heart (RPKM 1078.7) and 1 other tissue See more
Orthologs
human mouse all
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Genomic context

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Location:
7q36
Exon count:
26
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 7 NC_086025.1 (131100684..131138250)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 7 NC_051342.1 (129221679..129259192)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 7 NC_005106.4 (139702066..139722132)

Chromosome 7 - NC_086025.1Genomic Context describing neighboring genes Neighboring gene collagen type II alpha 1 chain Neighboring gene SUMO specific peptidase 1 Neighboring gene small nucleolar RNA SNORA17 Neighboring gene uncharacterized LOC134479569 Neighboring gene transfer RNA threonine (anticodon AGU) 34 Neighboring gene uncharacterized LOC120093732 Neighboring gene ankyrin repeat and SOCS box-containing 8 Neighboring gene coiled-coil domain containing 184

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. The up regulation of phosphofructokinase1 (PFK1) protein during chemically induced hypoxia is mediated by the hypoxia-responsive internal ribosome entry site (IRES) element, present in its 5'untranslated region. Ismail R, et al. Biochimie, 2017 Aug. PMID 28545955
  2. Expression of key substrate cycle enzymes in rat spermatogenic cells: fructose 1,6 bisphosphatase and 6 phosphofructose 1-kinase. Yáñez AJ, et al. J Cell Physiol, 2007 Sep. PMID 17492776
  3. Impairment of glucose metabolism in hearts from rats treated with endotoxin. Tessier JP, et al. Cardiovasc Res, 2003 Oct 15. PMID 14522413
  4. Characterization of expression of phosphofructokinase isoforms in isolated rat pancreatic islets and purified beta cells and cloning and expression of the rat phosphofructokinase-A isoform. Ma Z, et al. Biochim Biophys Acta, 1996 Aug 14. PMID 8764833
  5. Rapid changes in carbohydrate metabolism in muscle induced by triiodothyronine; the role of glucose 1,6-bisphosphate. Chen-Zion M, et al. Biochem Mol Med, 1995 Oct. PMID 8593533

See all (37) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. High phosphofructokinase expression is associated with glioma.
    Title: The up regulation of phosphofructokinase1 (PFK1) protein during chemically induced hypoxia is mediated by the hypoxia-responsive internal ribosome entry site (IRES) element, present in its 5'untranslated region.
  2. Coordinated expression of PKF-A/FBPase controls sperm cell glucose metabolism and sperm development and function.
    Title: Expression of key substrate cycle enzymes in rat spermatogenic cells: fructose 1,6 bisphosphatase and 6 phosphofructose 1-kinase.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables 6-phosphofructokinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 6-phosphofructokinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 6-phosphofructokinase activity IEA
Inferred from Electronic Annotation
more info
  
enables 6-phosphofructokinase activity ISO
Inferred from Sequence Orthology
more info
  
enables 6-phosphofructokinase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables AMP binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables AMP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding ISO
Inferred from Sequence Orthology
more info
  
enables carbohydrate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables fructose binding IEA
Inferred from Electronic Annotation
more info
  
enables fructose binding ISO
Inferred from Sequence Orthology
more info
  
enables fructose-6-phosphate binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables fructose-6-phosphate binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables identical protein binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables kinase binding IEA
Inferred from Electronic Annotation
more info
  
enables kinase binding ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables monosaccharide binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables phosphofructokinase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables phosphofructokinase activity ISO
Inferred from Sequence Orthology
more info
  
enables phosphofructokinase activity NAS
Non-traceable Author Statement
more info
 PubMed 
Process Evidence Code Pubs
involved_in canonical glycolysis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in canonical glycolysis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within canonical glycolysis ISO
Inferred from Sequence Orthology
more info
  
involved_in canonical glycolysis ISO
Inferred from Sequence Orthology
more info
  
involved_in carbohydrate phosphorylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within carbohydrate phosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in fructose 1,6-bisphosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fructose 1,6-bisphosphate metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in fructose 6-phosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fructose 6-phosphate metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in fructose 6-phosphate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in fructose 6-phosphate metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in glucose homeostasis IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glucose homeostasis ISO
Inferred from Sequence Orthology
more info
  
involved_in glycogen catabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glycogen catabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in glycolysis from storage polysaccharide through glucose-1-phosphate IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within glycolysis from storage polysaccharide through glucose-1-phosphate ISO
Inferred from Sequence Orthology
more info
  
involved_in glycolytic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in glycolytic process ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within glycolytic process through fructose-6-phosphate ISO
Inferred from Sequence Orthology
more info
  
involved_in glycolytic process through fructose-6-phosphate ISO
Inferred from Sequence Orthology
more info
  
involved_in intracellular glucose homeostasis NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in muscle cell cellular homeostasis IEA
Inferred from Electronic Annotation
more info
  
involved_in muscle cell cellular homeostasis ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of insulin secretion IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within positive regulation of insulin secretion ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of transcription by RNA polymerase II ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
part_of 6-phosphofructokinase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of 6-phosphofructokinase complex IEA
Inferred from Electronic Annotation
more info
  
part_of 6-phosphofructokinase complex ISO
Inferred from Sequence Orthology
more info
  
located_in apical plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in apical plasma membrane ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
is_active_in membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
located_in sperm principal piece IEA
Inferred from Electronic Annotation
more info
  
located_in sperm principal piece ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
ATP-dependent 6-phosphofructokinase, muscle type
Names
6-phosphofructokinase type A
6-phosphofructokinase, muscle type
phosphofructo-1-kinase isozyme A
phosphofructokinase 1
phosphofructokinase-A
phosphofructokinase-M
phosphohexokinase
NP_113903.2
XP_038935791.1
XP_063120288.1
XP_063120289.1
XP_063120290.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_031715.2 → NP_113903.2  ATP-dependent 6-phosphofructokinase, muscle type

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000007
    UniProtKB/Swiss-Prot
    P47858, Q63736
    UniProtKB/TrEMBL
    A0A0G2JY38, A6KC58, Q52KS1
    Related
    ENSRNOP00000070507.1, ENSRNOT00000078579.2
    Conserved Domains (1) summary
    cd00764
    Location:13 → 774
    Eukaryotic_PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to a subfamily of the PFKA family (cd00363) and include eukaryotic ATP-dependent ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086025.1 Reference GRCr8

    Range
    131100684..131138250
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_063264219.1 → XP_063120289.1  ATP-dependent 6-phosphofructokinase, muscle type isoform X1

    UniProtKB/TrEMBL
    A0A0G2KBC7

  2. XM_039079863.2 → XP_038935791.1  ATP-dependent 6-phosphofructokinase, muscle type isoform X1

    UniProtKB/TrEMBL
    A0A0G2KBC7, A0A8I6ADR0
    Related
    ENSRNOP00000075742.1
    Conserved Domains (1) summary
    cd00764
    Location:84 → 845
    Eukaryotic_PFK; Phosphofructokinase, a key regulatory enzyme in glycolysis, catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-biphosphate. The members belong to a subfamily of the PFKA family (cd00363) and include eukaryotic ATP-dependent ...

  3. XM_063264220.1 → XP_063120290.1  ATP-dependent 6-phosphofructokinase, muscle type isoform X1

    UniProtKB/TrEMBL
    A0A0G2KBC7

  4. XM_063264218.1 → XP_063120288.1  ATP-dependent 6-phosphofructokinase, muscle type isoform X1

    UniProtKB/TrEMBL
    A0A0G2KBC7
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P47858.3 GenPept UniProtKB/Swiss-Prot:P47858

Gene LinkOut

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