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Nckap1 NCK-associated protein 1 [ Mus musculus (house mouse) ]

Gene ID: 50884, updated on 25-Apr-2024

Summary

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Official Symbol
Nckap1provided by MGI
Official Full Name
NCK-associated protein 1provided by MGI
Primary source
MGI:MGI:1355333
See related
Ensembl:ENSMUSG00000027002 AllianceGenome:MGI:1355333
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
H19; Hem2; Nap1; mh19; Hem-2; p125Nap1; mKIAA0587
Summary
Predicted to contribute to small GTPase binding activity. Involved in positive regulation of actin filament polymerization and positive regulation of lamellipodium assembly. Acts upstream of or within several processes, including apical protein localization; embryonic morphogenesis; and segmentation. Located in lamellipodium and ruffle. Part of filamentous actin. Is active in postsynapse. Is expressed in several structures, including alimentary system; genitourinary system; immune system; nervous system; and sensory organ. Orthologous to human NCKAP1 (NCK associated protein 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in cortex adult (RPKM 59.6), frontal lobe adult (RPKM 57.9) and 26 other tissues See more
Orthologs
human all
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Genomic context

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Location:
2 C3; 2 48.21 cM
Exon count:
32
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (80330856..80411526, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (80500512..80581182, complement)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_04946 Neighboring gene STARR-seq mESC enhancer starr_04947 Neighboring gene STARR-seq mESC enhancer starr_04948 Neighboring gene frizzled-related protein Neighboring gene mitochondrial ribosomal protein S36 pseudogene Neighboring gene N-glycanase 1 pseudogene Neighboring gene predicted gene, 24461 Neighboring gene predicted gene 13688 Neighboring gene STARR-positive B cell enhancer ABC_E7830 Neighboring gene dual specificity phosphatase 19 Neighboring gene microRNA 684-1 Neighboring gene SMT3 suppressor of mif two 3 homolog 2 pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Nap1-mediated actin remodeling is essential for mammalian myoblast fusion. Nowak SJ, et al. J Cell Sci, 2009 Sep 15. PMID 19706686, Free PMC Article
  2. The RAC1 Target NCKAP1 Plays a Crucial Role in the Progression of Braf;Pten-Driven Melanoma in Mice. Swaminathan K, et al. J Invest Dermatol, 2021 Mar. PMID 32777214
  3. The WAVE Regulatory Complex Is Required to Balance Protrusion and Adhesion in Migration. Whitelaw JA, et al. Cells, 2020 Jul 7. PMID 32646006, Free PMC Article
  4. Nap1-regulated neuronal cytoskeletal dynamics is essential for the final differentiation of neurons in cerebral cortex. Yokota Y, et al. Neuron, 2007 May 3. PMID 17481396, Free PMC Article
  5. NCK Associated Protein 1 Modulated by miRNA-214 Determines Vascular Smooth Muscle Cell Migration, Proliferation, and Neointima Hyperplasia. Afzal TA, et al. J Am Heart Assoc, 2016 Dec 7. PMID 27927633, Free PMC Article

See all (59) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The RAC1 Target NCKAP1 Plays a Crucial Role in the Progression of Braf;Pten-Driven Melanoma in Mice.
    Title: The RAC1 Target NCKAP1 Plays a Crucial Role in the Progression of Braf;Pten-Driven Melanoma in Mice.
  2. Inhibition of Nap1, a member of the WAVE actin-remodeling complex, results in accumulations of F-actin structures at the plasma membrane that are concomitant with a decrease in myoblast fusion.
    Title: Nap1-mediated actin remodeling is essential for mammalian myoblast fusion.
  3. Nap1 mutant phenotypes define the crucial roles of Nap1/WAVE-mediated actin regulation in tissue organization and establishment of the body plan of the mammalian embryo.
    Title: Axis specification and morphogenesis in the mouse embryo require Nap1, a regulator of WAVE-mediated actin branching.
  4. Nap1 is a constitutive and essential component of a WAVE2- and Abi-1-containing complex linking Rac to site-directed actin assembly.
    Title: Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
contributes_to small GTPase binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in Rac protein signal transduction ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within apical protein localization IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within basal protein localization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cell migration IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within cell migration involved in gastrulation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in cell morphogenesis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cell projection assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cortical actin cytoskeleton organization IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within embryonic body morphogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within embryonic foregut morphogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within embryonic heart tube development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within endoderm development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within establishment or maintenance of actin cytoskeleton polarity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within in utero embryonic development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within lamellipodium assembly IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within lamellipodium assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within mesodermal cell migration IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within neural tube closure IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in neuron projection morphogenesis IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within notochord development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within notochord morphogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within paraxial mesoderm development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within paraxial mesoderm morphogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of Arp2/3 complex-mediated actin nucleation ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of actin filament polymerization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of lamellipodium assembly IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of lamellipodium assembly ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within protein stabilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within regulation of protein localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within somitogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within zygotic determination of anterior/posterior axis, embryo IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of SCAR complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of SCAR complex ISO
Inferred from Sequence Orthology
more info
  
located_in cell projection IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
part_of filamentous actin IDA
Inferred from Direct Assay
more info
 PubMed 
located_in lamellipodium IDA
Inferred from Direct Assay
more info
 PubMed 
located_in membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
is_active_in postsynapse IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in postsynapse IEP
Inferred from Expression Pattern
more info
 PubMed 
located_in ruffle IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
nck-associated protein 1
Names
brain protein H19
membrane-associated protein HEM-2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001290745.1 → NP_001277674.1  nck-associated protein 1 isoform a

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).
    Source sequence(s)
    BC060197, BC127067, BP764873, BU055892
    Consensus CDS
    CCDS71082.1
    UniProtKB/TrEMBL
    A1L0U6, A2AS98
    Related
    ENSMUSP00000107390.3, ENSMUST00000111760.3
    Conserved Domains (1) summary
    pfam09735
    Location:13 → 1128
    Nckap1; Membrane-associated apoptosis protein

  2. NM_001355410.1 → NP_001342339.1  nck-associated protein 1 isoform c

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) uses an alternate in-frame splice junction in the 5' end compared to variant 1, resulting in an isoform (c) that is shorter compared to isoform a.
    Source sequence(s)
    AL928578
    UniProtKB/TrEMBL
    A1L0U6
    Conserved Domains (1) summary
    pfam09735
    Location:13 → 1126
    Nckap1; Membrane-associated apoptosis protein

  3. NM_001355411.1 → NP_001342340.1  nck-associated protein 1 isoform d

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) lacks an alternate in-frame exon and uses an alternate in-frame splice junction in the 5' end compared to variant 1, resulting in an isoform (d) that is shorter compared to isoform a.
    Source sequence(s)
    AL928578
    UniProtKB/TrEMBL
    A1L0U6
    Conserved Domains (1) summary
    pfam09735
    Location:13 → 1120
    Nckap1; Membrane-associated apoptosis protein

  4. NM_016965.3 → NP_058661.1  nck-associated protein 1 isoform b

    See identical proteins and their annotated locations for NP_058661.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' end compared to variant 1, resulting in an isoform (b) that is shorter compared to isoform a.
    Source sequence(s)
    AJ534525, BC060197, BP764873, BU055892
    Consensus CDS
    CCDS16177.1
    UniProtKB/Swiss-Prot
    P28660, Q3UPY6, Q80TX0, Q8CG49, Q99KY0
    UniProtKB/TrEMBL
    A1L0U6
    Related
    ENSMUSP00000028386.6, ENSMUST00000028386.12
    Conserved Domains (1) summary
    pfam09735
    Location:13 → 1122
    Nckap1; Membrane-associated apoptosis protein

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    80330856..80411526 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P28660.2 GenPept UniProtKB/Swiss-Prot:P28660

Gene LinkOut

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