rock2a rho-associated, coiled-coil containing protein kinase 2a [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: rock2aprovided by ZNC
Official Full Name: rho-associated, coiled-coil containing protein kinase 2aprovided by ZNC
Primary source: ZFIN:ZDB-GENE-030115-3
See related: Ensembl:ENSDARG00000017500 AllianceGenome:ZFIN:ZDB-GENE-030115-3
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: rok2; rock2
Summary: Predicted to enable Rho-dependent protein serine/threonine kinase activity. Acts upstream of or within several processes, including gastrulation; protein catabolic process in the vacuole; and regulation of actin cytoskeleton organization. Located in plasma membrane. Is expressed in corneal epithelium; fin bud; midbrain hindbrain boundary; otic vesicle; and pharyngeal arch 3-7. Orthologous to human ROCK2 (Rho associated coiled-coil containing protein kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Annotation information: Annotation category: partial on reference assembly
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 13

Exon count:: 31

Annotation release	Status	Assembly	Chr	Location
106	current	GRCz11 (GCF_000002035.6)	13	NC_007124.7 (32815243..32899047, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	13	NC_007124.6 (32684793..32768597, complement)

Chromosome 13 - NC_007124.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The findings of the present study indicate that miR-142-3p plays a critical role in hematopoiesis, cardiogenesis, and somitegenesis in the early stage of mesoderm formation via regulation of Rock2a.
Title: miR-142-3p is essential for hematopoiesis and affects cardiac cell fate in zebrafish.
Double knockdown of rock2a disrupted interkinetic nuclear migration in the retina.
Title: Actin-Cytoskeleton- and Rock-Mediated INM Are Required for Photoreceptor Regeneration in the Adult Zebrafish Retina.
Data show that Rock2 acts as a negative regulator of the TGFbeta signaling pathway.
Title: Rock2 controls TGFbeta signaling and inhibits mesoderm induction in zebrafish embryos.

Submit: New GeneRIF Correction

General gene information

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Markers

fc43d12.x1 (e-PCR)
- UniSTS:193854

fb71c08.x1 (e-PCR)
- UniSTS:191051

fb71c08.x1 (e-PCR)
- UniSTS:191052

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables Rho-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within Rho protein signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within Wnt signaling pathway	IMP Inferred from Mutant Phenotype more info
acts_upstream_of_or_within actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
involved_in actomyosin structure organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell migration involved in gastrulation	IMP Inferred from Mutant Phenotype more info
involved_in cortical actin cytoskeleton organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in embryonic morphogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within heart morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within mediolateral intercalation	IMP Inferred from Mutant Phenotype more info
acts_upstream_of_or_within mesodermal cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic cytokinesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in peptidyl-threonine phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within planar cell polarity pathway involved in gastrula mediolateral intercalation	IMP Inferred from Mutant Phenotype more info
acts_upstream_of_or_within positive regulation of centrosome duplication	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein catabolic process	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within protein catabolic process in the vacuole	IPI Inferred from Physical Interaction more info	PubMed
acts_upstream_of_or_within regulation of actin cytoskeleton organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell junction assembly	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within rhythmic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within smooth muscle contraction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: rho-associated protein kinase 2

Names: rho-associated, coiled-coil containing protein kinase 2

NP_777288.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_174863.3 → NP_777288.1 rho-associated protein kinase 2

See identical proteins and their annotated locations for NP_777288.1

Status: VALIDATED

Source sequence(s)

CR936241

UniProtKB/TrEMBL

A0A8N7UZU0, Q90Y37

Related

ENSDARP00000122621.1, ENSDART00000133882.3

Conserved Domains (7) summary

cd11638
Location:491 → 557

HR1_ROCK2; Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Rho-associated coiled-coil containing protein kinase 2

smart00220
Location:78 → 340

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd00029
Location:1250 → 1304

C1; Protein kinase C conserved region 1 (C1) . Cysteine-rich zinc binding domain. Some members of this domain family bind phorbol esters and diacylglycerol, some are reported to bind RasGTP. May occur in tandem arrangement. Diacylglycerol (DAG) is a second ...

cd01242
Location:1141 → 1246

PH_ROCK; Rho-associated coiled-coil containing protein kinase pleckstrin homology (PH) domain

cd05621
Location:25 → 403

STKc_ROCK2; Catalytic domain of the Serine/Threonine Kinase, Rho-associated coiled-coil containing protein kinase 2

pfam08912
Location:965 → 1032

Rho_Binding; Rho Binding

pfam15450
Location:431 → 1028

DUF4631; Domain of unknown function (DUF4631)