Clca1 chloride channel accessory 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Clca1provided by MGI
Official Full Name: chloride channel accessory 1provided by MGI
Primary source: MGI:MGI:1346342
See related: Ensembl:ENSMUSG00000028255 AllianceGenome:MGI:1346342
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: gob5; Clca2; Clca3; gob-5
Summary: Predicted to enable intracellular calcium activated chloride channel activity. Predicted to act upstream of or within chloride transport. Located in microvillus and secretory granule. Is integral component of membrane. Is expressed in genitourinary system; heart; rectum lumen; retina; and thymus. Human ortholog(s) of this gene implicated in asthma and chronic obstructive pulmonary disease. Orthologous to human CLCA1 (chloride channel accessory 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in colon adult (RPKM 313.3), large intestine adult (RPKM 280.7) and 3 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 H2; 3 69.26 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (144710298..144738537, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (145004537..145032776, complement)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

CLCA1 Regulates Airway Mucus Production and Ion Secretion Through TMEM16A.
Title: CLCA1 Regulates Airway Mucus Production and Ion Secretion Through TMEM16A.
SDR16C5 and SDR16C6 enzymes are not critical for survival but are responsible for most of the retinol dehydrogenase activity in skin, essential for the regulation of the hair-follicle cycle, and required for the maintenance of both sebaceous and meibomian glands
Title: Calcium-activated chloride channel regulator 1 (CLCA1) forms non-covalent oligomers in colonic mucus and has mucin 2-processing properties.
Results suggest that CLCA1 is involved in intestinal mucus homeostasis by facilitating processing and removal of mucus to prevent stagnation.
Title: Calcium-activated Chloride Channel Regulator 1 (CLCA1) Controls Mucus Expansion in Colon by Proteolytic Activity.
The data underscore and specify the role of mouse CLCA1 in inflammatory airway disease to activate airway macrophages.
Title: Soluble mucus component CLCA1 modulates expression of leukotactic cytokines and BPIFA1 in murine alveolar macrophages but not in bone marrow-derived macrophages.
These findings demonstrate that 10.1.1 Ab rapidly and specifically induces proliferation and growth of Lymph Node lymphatic sinuses and stroma, suggesting a key role of mCLCA1 in coordinating Lymph Node remodeling during immune responses.
Title: The Lymphatic Endothelial mCLCA1 Antibody Induces Proliferation and Growth of Lymph Node Lymphatic Sinuses.
Clca1 is not required for mucus synthesis, structure and barrier function in the murine colon.
Title: The Goblet Cell Protein Clca1 (Alias mClca3 or Gob-5) Is Not Required for Intestinal Mucus Synthesis, Structure and Barrier Function in Naive or DSS-Challenged Mice.
mCLCA3 appears to modulate leukocyte response via IL-17 and murine CXCL-8 homologs in acute Staphylococcus aureus pneumonia which is well in line with the proposed function of hCLCA1 as a signaling molecule acting on alveolar macrophages.
Title: mCLCA3 modulates IL-17 and CXCL-1 induction and leukocyte recruitment in murine Staphylococcus aureus pneumonia.
Findings suggest that increased expression of a single-gene, mCLCA3, could simulate an asthma attack, and its mechanism may involve mCLCA3 overexpression up-regulating IL-13 expression.
Title: Murine calcium-activated chloride channel family member 3 induces asthmatic airway inflammation independently of allergen exposure.
PA-LPS-induced MUC5AC and Clca3 expression is partly through Duox1
Title: P. aeruginosa lipopolysaccharide-induced MUC5AC and CLCA3 expression is partly through Duox1 in vitro and in vivo.
these findings argue against a role of mCLCA3 in Ca(2 )-activated Cl(-) channel-mediated Cl(-) secretion in murine respiratory epithelia
Title: mCLCA3 does not contribute to calcium-activated chloride conductance in murine airways.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (4)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables chloride channel activity	ISA Inferred from Sequence Alignment more info	PubMed
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables intracellularly calcium-gated chloride channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables peptidase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within calcium ion transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chloride transport	ISA Inferred from Sequence Alignment more info	PubMed
acts_upstream_of_or_within monoatomic ion transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within proteolysis	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in microvillus	IDA Inferred from Direct Assay more info	PubMed
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in secretory granule	IDA Inferred from Direct Assay more info	PubMed
located_in zymogen granule membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: calcium-activated chloride channel regulator 1

Names: calcium-activated chloride channel family member 3; chloride channel calcium activated 1; chloride channel calcium activated 2; chloride channel calcium activated 3; mCLCA3; protein gob-5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017474.2 → NP_059502.1 calcium-activated chloride channel regulator 1 precursor

See identical proteins and their annotated locations for NP_059502.1

Status: VALIDATED

Source sequence(s)

AK008659, AK033560

Consensus CDS

CCDS17889.1

UniProtKB/Swiss-Prot

O88826, Q8R049, Q9D7Z6

Related

ENSMUSP00000029919.6, ENSMUST00000029919.7

Conserved Domains (2) summary

cd00198
Location:306 → 459

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...

pfam08434
Location:25 → 907

CLCA; Calcium-activated chloride channel

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000069.7 Reference GRCm39 C57BL/6J

Range

144710298..144738537 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006501444.1 → XP_006501507.1 calcium-activated chloride channel regulator 1 isoform X1

Conserved Domains (1) summary

TIGR00868
Location:1 → 573

hCaCC; calcium-activated chloride channel protein 1