Arhgap35 Rho GTPase activating protein 35 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Arhgap35provided by MGI
Official Full Name: Rho GTPase activating protein 35provided by MGI
Primary source: MGI:MGI:1929494
See related: Ensembl:ENSMUSG00000058230 AllianceGenome:MGI:1929494
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Grlf1; p190A; mKIAA1722; p190RhoGAP; 6430596G11Rik
Summary: Enables GTPase activator activity. Involved in several processes, including axon development; establishment or maintenance of actin cytoskeleton polarity; and regulation of plasma membrane bounded cell projection organization. Acts upstream of or within several processes, including negative regulation of Rho protein signal transduction; negative regulation of vascular permeability; and nervous system development. Located in actin cytoskeleton; ciliary basal body; and cytoplasm. Is expressed in central nervous system and eye. Orthologous to human ARHGAP35 (Rho GTPase activating protein 35). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in whole brain E14.5 (RPKM 20.1), ovary adult (RPKM 18.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 A2; 7 9.15 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (16228398..16349313, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (16494473..16614993, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HNRNPL Circularizes ARHGAP35 to Produce an Oncogenic Protein.
Title: HNRNPL Circularizes ARHGAP35 to Produce an Oncogenic Protein.
p190RhoGAP is a critical regulator of motor axon guidance. Rather than acting as a compulsory signal relay, p190RhoGAP uses a non-conventional GAP-independent mode to transiently suppress attraction to Netrin-1 while motor axons exit the spinal cord. Once in the periphery, a subset of axons requires p190RhoGAP-mediated inhibition of Rho signaling to target specific muscles.
Title: p190RhoGAP Filters Competing Signals to Resolve Axon Guidance Conflicts.
p190A acts as a modulator of Rho GTPases in a localized area around the cilia to permit the dynamic actin rearrangement required for cilia elongation.
Title: A Point Mutation in p190A RhoGAP Affects Ciliogenesis and Leads to Glomerulocystic Kidney Defects.
The cellular RacGAP activity of p190RhoGAP requires an intact polybasic region adjacent to the GAP domain whereas the RhoGAP activity is inhibited by the same domain.
Title: p190RhoGAP has cellular RacGAP activity regulated by a polybasic region.
study demonstrates that the epidermal growth factor receptor (EGFR) cooperates with beta3 integrin to regulate p190RhoGAP activity in mammary gland epithelial cells
Title: β3 integrin-EGF receptor cross-talk activates p190RhoGAP in mouse mammary gland epithelial cells.
p190RhoGAP links integrins and caveolin-1/caveolae to RhoA in a mechanotransduction cascade that participates in endothelial adaptation to flow.
Title: p190 RhoGTPase-activating protein links the β1 integrin/caveolin-1 mechanosignaling complex to RhoA and actin remodeling.
In a novel knockout mouse strain p190RhoGAP does not play a major, indispensable role in integrin signaling of neutrophils.
Title: Neutrophil functions and autoimmune arthritis in the absence of p190RhoGAP: generation and analysis of a novel null mutation in mice.
Cdh1 formed a physical complex with p190 and stimulated the efficient ubiquitination of p190, both in in vitro and in vivo.
Title: The anaphase-promoting complex/cyclosome activator Cdh1 modulates Rho GTPase by targeting p190 RhoGAP for degradation.
Data show that fibroblast, endothelial and carcinoma polarity during cell migration requires FAK and is associated with a complex between FAK, p120RasGAP and p190RhoGAP (p190A), leading to p190A tyrosine phosphorylation.
Title: A FAK-p120RasGAP-p190RhoGAP complex regulates polarity in migrating cells.
p190RhoGAP as the convergence point for adhesive signals mediated by alpha(5)beta(1) integrin and syndecan-4.
Title: p190RhoGAP is the convergence point of adhesion signals from alpha 5 beta 1 integrin and syndecan-4.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Chemically induced (ENU) (2) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:235604

AI841135 (e-PCR)
- UniSTS:165509

BF405601 (e-PCR)
- UniSTS:247944

RH132160 (e-PCR)
- UniSTS:215443

D7Mit342 (e-PCR), detects polymorphism
- UniSTS:131241

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activating protein binding	ISO Inferred from Sequence Orthology more info
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activator activity	ISO Inferred from Sequence Orthology more info
enables GTPase activator activity	TAS Traceable Author Statement more info	PubMed
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in Rho protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of Rho protein signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in axon guidance	IDA Inferred from Direct Assay more info	PubMed
involved_in axonal fasciculation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within camera-type eye development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in central nervous system neuron axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment or maintenance of actin cytoskeleton polarity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within forebrain development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within integrin-mediated signaling pathway	TAS Traceable Author Statement more info	PubMed
involved_in mammary gland development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of Rho protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of Rho protein signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within negative regulation of vascular permeability	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neural tube closure	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron projection guidance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cilium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of neuron projection development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of neuron projection development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actin polymerization or depolymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of axonogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of cell shape	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell size	IBA Inferred from Biological aspect of Ancestor more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in wound healing, spreading of cells	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in actin cytoskeleton	IDA Inferred from Direct Assay more info	PubMed
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in ciliary basal body	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: rho GTPase-activating protein 35

Names: P190 RhoGAP; glucocorticoid receptor DNA-binding factor 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_172739.4 → NP_766327.3 rho GTPase-activating protein 35

See identical proteins and their annotated locations for NP_766327.3

Status: VALIDATED

Source sequence(s)

AK032559, AK136766, AK137250, AK147326

Consensus CDS

CCDS20851.1

UniProtKB/Swiss-Prot

Q3UGY1, Q69ZC4, Q91YM2

UniProtKB/TrEMBL

B2RTN5

Related

ENSMUSP00000075242.5, ENSMUST00000075845.11

Conserved Domains (5) summary

smart00441
Location:431 → 483

FF; Contains two conserved F residues

smart00173
Location:158 → 250

RAS; Ras subfamily of RAS small GTPases

cd04373
Location:1247 → 1431

RhoGAP_p190; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an ...

pfam16512
Location:261 → 340

RhoGAP-FF1; p190-A and -B Rho GAPs FF domain

cd22221
Location:594 → 765

pseudoGTPaseD_p190RhoGAP-A; pseudoGTPase domain found in p190RhoGAP-A and similar proteins

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

16228398..16349313 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006539809.5 → XP_006539872.1 rho GTPase-activating protein 35 isoform X1

See identical proteins and their annotated locations for XP_006539872.1

UniProtKB/Swiss-Prot

Q3UGY1, Q69ZC4, Q91YM2

UniProtKB/TrEMBL

B2RTN5

Conserved Domains (5) summary

smart00441
Location:431 → 483

FF; Contains two conserved F residues

smart00173
Location:158 → 250

RAS; Ras subfamily of RAS small GTPases

cd04373
Location:1247 → 1431

RhoGAP_p190; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an ...

pfam16512
Location:261 → 340

RhoGAP-FF1; p190-A and -B Rho GAPs FF domain

cd22221
Location:594 → 765

pseudoGTPaseD_p190RhoGAP-A; pseudoGTPase domain found in p190RhoGAP-A and similar proteins
XM_036152946.1 → XP_036008839.1 rho GTPase-activating protein 35 isoform X2

UniProtKB/TrEMBL

Q3TSU7

Conserved Domains (5) summary

smart00441
Location:431 → 483

FF; Contains two conserved F residues

smart00173
Location:158 → 250

RAS; Ras subfamily of RAS small GTPases

pfam16512
Location:261 → 340

RhoGAP-FF1; p190-A and -B Rho GAPs FF domain

cd22221
Location:594 → 765

pseudoGTPaseD_p190RhoGAP-A; pseudoGTPase domain found in p190RhoGAP-A and similar proteins

cl02570
Location:1247 → 1361

RhoGAP; GTPase-activator protein (GAP) for Rho-like GTPases; GAPs towards Rho/Rac/Cdc42-like small GTPases. Small GTPases (G proteins) cluster into distinct families, and all act as molecular switches, active in their GTP-bound form but inactive when bound to ...
XM_036152945.1 → XP_036008838.1 rho GTPase-activating protein 35 isoform X1

UniProtKB/Swiss-Prot

Q3UGY1, Q69ZC4, Q91YM2

UniProtKB/TrEMBL

B2RTN5

Conserved Domains (5) summary

smart00441
Location:431 → 483

FF; Contains two conserved F residues

smart00173
Location:158 → 250

RAS; Ras subfamily of RAS small GTPases

cd04373
Location:1247 → 1431

RhoGAP_p190; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an ...

pfam16512
Location:261 → 340

RhoGAP-FF1; p190-A and -B Rho GAPs FF domain

cd22221
Location:594 → 765

pseudoGTPaseD_p190RhoGAP-A; pseudoGTPase domain found in p190RhoGAP-A and similar proteins
XM_011250512.4 → XP_011248814.1 rho GTPase-activating protein 35 isoform X1

See identical proteins and their annotated locations for XP_011248814.1

UniProtKB/Swiss-Prot

Q3UGY1, Q69ZC4, Q91YM2

UniProtKB/TrEMBL

B2RTN5

Conserved Domains (5) summary

smart00441
Location:431 → 483

FF; Contains two conserved F residues

smart00173
Location:158 → 250

RAS; Ras subfamily of RAS small GTPases

cd04373
Location:1247 → 1431

RhoGAP_p190; RhoGAP (GTPase-activator protein [GAP] for Rho-like small GTPases) domain of p190-like proteins. p190, also named RhoGAP5, plays a role in neuritogenesis and axon branch stability. p190 shows a preference for Rho, over Rac and Cdc42, and consists of an ...

pfam16512
Location:261 → 340

RhoGAP-FF1; p190-A and -B Rho GAPs FF domain

cd22221
Location:594 → 765

pseudoGTPaseD_p190RhoGAP-A; pseudoGTPase domain found in p190RhoGAP-A and similar proteins