Pck1 phosphoenolpyruvate carboxykinase 1, cytosolic [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pck1provided by MGI
Official Full Name: phosphoenolpyruvate carboxykinase 1, cytosolicprovided by MGI
Primary source: MGI:MGI:97501
See related: Ensembl:ENSMUSG00000027513 AllianceGenome:MGI:97501
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: PEPCK; Pck-1; PEPCK-C
Summary: Enables phosphoenolpyruvate carboxykinase (GTP) activity. Involved in oxaloacetate metabolic process and positive regulation of memory T cell differentiation. Acts upstream of or within several processes, including cellular response to potassium ion starvation; gluconeogenesis; and glycerol biosynthetic process from pyruvate. Located in cytosol. Is expressed in several structures, including alimentary system; brown fat; liver; and urinary system. Human ortholog(s) of this gene implicated in Alzheimer's disease and type 2 diabetes mellitus. Orthologous to human PCK1 (phosphoenolpyruvate carboxykinase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in kidney adult (RPKM 295.2), liver adult (RPKM 158.6) and 6 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 H3; 2 95.79 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (172994866..173001067)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (173153073..173159274)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

hnRNPA2B1 promotes the occurrence and progression of hepatocellular carcinoma by downregulating PCK1 mRNA via a m6A RNA methylation manner.
Title: hnRNPA2B1 promotes the occurrence and progression of hepatocellular carcinoma by downregulating PCK1 mRNA via a m6A RNA methylation manner.
PCK1 Protects against Mitoribosomal Defects in Diabetic Nephropathy in Mouse Models.
Title: PCK1 Protects against Mitoribosomal Defects in Diabetic Nephropathy in Mouse Models.
Hepatic ZBTB22 promotes hyperglycemia and insulin resistance via PEPCK1-driven gluconeogenesis.
Title: Hepatic ZBTB22 promotes hyperglycemia and insulin resistance via PEPCK1-driven gluconeogenesis.
TRIM21 ameliorates hepatic glucose and lipid metabolic disorders in type 2 diabetes mellitus by ubiquitination of PEPCK1 and FASN.
Title: TRIM21 ameliorates hepatic glucose and lipid metabolic disorders in type 2 diabetes mellitus by ubiquitination of PEPCK1 and FASN.
PCK1 is a key regulator of metabolic and mitochondrial functions in renal tubular cells.
Title: PCK1 is a key regulator of metabolic and mitochondrial functions in renal tubular cells.
Deficiency of gluconeogenic enzyme PCK1 promotes metabolic-associated fatty liver disease through PI3K/AKT/PDGF axis activation in male mice.
Title: Deficiency of gluconeogenic enzyme PCK1 promotes metabolic-associated fatty liver disease through PI3K/AKT/PDGF axis activation in male mice.
LncRNA Ctcflos modulates glucocorticoid receptor-mediated induction of hepatic phosphoenolpyruvate carboxykinase in mice.
Title: LncRNA Ctcflos modulates glucocorticoid receptor-mediated induction of hepatic phosphoenolpyruvate carboxykinase in mice.
Multitissue 2H/13C flux analysis reveals reciprocal upregulation of renal gluconeogenesis in hepatic PEPCK-C-knockout mice.
Title: Multitissue 2H/13C flux analysis reveals reciprocal upregulation of renal gluconeogenesis in hepatic PEPCK-C-knockout mice.
Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation.
Title: Gluconeogenic enzyme PCK1 deficiency promotes CHK2 O-GlcNAcylation and hepatocellular carcinoma growth upon glucose deprivation.
Cytosolic phosphoenolpyruvate carboxykinase is expressed in alpha-cells from human and murine pancreas.
Title: Cytosolic phosphoenolpyruvate carboxykinase is expressed in α-cells from human and murine pancreas.

Submit: New GeneRIF Correction See all GeneRIFs (46)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (7) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Pck1 (e-PCR), detects polymorphism
- UniSTS:144137

PMC133887P2 (e-PCR)
- UniSTS:270676

Pck1 (e-PCR), detects polymorphism
- UniSTS:461927

AI265463 (e-PCR)
- UniSTS:159082

PMC152262P3 (e-PCR)
- UniSTS:271211

Pck1 (e-PCR), detects polymorphism
- UniSTS:546923

PMC86125P1 (e-PCR)
- UniSTS:273518

d3arb24 (e-PCR)
- UniSTS:121771

Pck1 (e-PCR), detects polymorphism
- UniSTS:526051

PMC117356P1 (e-PCR)
- UniSTS:270330

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GDP binding	ISO Inferred from Sequence Orthology more info
enables GTP binding	ISO Inferred from Sequence Orthology more info
enables carboxy-lyase activity	IEA Inferred from Electronic Annotation more info
enables carboxylic acid binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables lyase activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables manganese ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables manganese ion binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleoside diphosphate kinase activity	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables phosphoenolpyruvate carboxykinase (GTP) activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphoenolpyruvate carboxykinase (GTP) activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphoenolpyruvate carboxykinase (GTP) activity	ISO Inferred from Sequence Orthology more info
enables phosphoenolpyruvate carboxykinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables phosphoenolpyruvate carboxykinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity (using GTP as donor)	ISO Inferred from Sequence Orthology more info
enables purine nucleotide binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular response to dexamethasone stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to glucose stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to glucose stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to insulin stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to insulin stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to potassium ion starvation	IDA Inferred from Direct Assay more info	PubMed
involved_in gluconeogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within gluconeogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in gluconeogenesis	IDA Inferred from Direct Assay more info	PubMed
involved_in gluconeogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in gluconeogenesis	ISO Inferred from Sequence Orthology more info
involved_in glucose homeostasis	ISO Inferred from Sequence Orthology more info
involved_in glucose metabolic process	ISO Inferred from Sequence Orthology more info
involved_in glyceraldehyde-3-phosphate biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycerol biosynthetic process from pyruvate	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within glycerol biosynthetic process from pyruvate	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glycerol biosynthetic process from pyruvate	ISO Inferred from Sequence Orthology more info
involved_in hepatocyte differentiation	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within lipid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oxaloacetate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in oxaloacetate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oxaloacetate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of lipid biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of memory T cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in propionate catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of lipid biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to bacterium	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to insulin	ISO Inferred from Sequence Orthology more info
involved_in response to starvation	IBA Inferred from Biological aspect of Ancestor more info
involved_in tricarboxylic acid metabolic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Names: phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxylase; serine-protein kinase PCK1

NP_035174.1

EC 4.1.1.32

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011044.3 → NP_035174.1 phosphoenolpyruvate carboxykinase, cytosolic [GTP]

See identical proteins and their annotated locations for NP_035174.1

Status: VALIDATED

Source sequence(s)

AL837509

Consensus CDS

CCDS17141.1

UniProtKB/Swiss-Prot

Q3UEH3, Q9Z2V4

UniProtKB/TrEMBL

Q8BSX3, Q8CI37

Related

ENSMUSP00000029017.6, ENSMUST00000029017.6

Conserved Domains (1) summary

cd00819
Location:27 → 615

PEPCK_GTP; Phosphoenolpyruvate carboxykinase (PEPCK), a critical gluconeogenic enzyme, catalyzes the first committed step in the diversion of tricarboxylic acid cycle intermediates toward gluconeogenesis. It catalyzes the reversible decarboxylation and ...