Relation Results

Summary

Name AKT
Primary ID SIGNOR-PF24
Type protein family
Formed by AKT1, AKT2, AKT3
Relations 394
Pathways Acute Myeloid Leukemia, ASXL1 in AML, BCR-ABL in AML, FLT3 in AML, KIT in AML, miRNA in AML, AML_TRIPLETS, AMPK Signaling, B-cell activation, COVID-19 Causal Network, EGFR Signaling, EBV infection, Eosinophil: IL5, ErbB receptors in cancer, FAP: In vitro induction of adipogenesis, FAP: Insulin-mediated adipogenesis, FAP: INSR, FLT3-ITD in AML, FLT3-ITD signaling, Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, Glioblastoma Multiforme, Glycolysis and Gluconeogenesis, Hepatocellular Tumor, Inflammosome Activation, Insulin Signaling, Inhibition of Apoptosis, Integrin Signaling, Luminal Breast Cancer, Leptin Signaling, Mast cell: SCF, mTOR in cancer, Mitochondrial dynamics in T cell exhaustion, Malignant Melanoma, MTOR Signaling, Myofiber: IL6 and atrophy, Myostatin, Triple mutant AML, NPM1_new, Non-small-cell lung cancer (NSCLC), Prostate Cancer, Pancreatic ductal adenocarcinoma (PDA), PI3K/AKT Signaling, Pericyte: In vitro induction of adipogenesis, Rhabdomyosarcoma, RTKs in cancer, SARS-COV APOPTOSIS, Satellite: IGF1R, SARS-CoV INFLAMMATORY RESPONSE, Thyroid cancer, T cell activation, Tenascin-C in PDAC, Thyroid Hormone Metabolism, VEGF Signaling, WNT/FLT3, YAP/TAZ regulation

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Type: Score: Layout: SPV 
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▶Modifications Tables

Relations
Regulator
Mechanism
target
score ℹ
+ SIRT1up-regulates activity img/direct-activation.png deacetylation AKT 0.618
Identifier Residue Sequence Organism Cell Line
SIGNOR-252456 Lys14 VKEGWLHkRGEYIKT Mus musculus
pmid sentence
We show that Akt and PDK1 are acetylated at lysine residues in their pleckstrin homology domains, which mediate PIP(3) binding. Acetylation blocked binding of Akt and PDK1 to PIP(3), thereby preventing membrane localization and phosphorylation of Akt. Deacetylation by SIRT1 enhanced binding of Akt and PDK1 to PIP(3) and promoted their activation.
Publications: 1 Organism: Mus Musculus
Tissue: Heart
Pathways:Acute Myeloid Leukemia, AMPK Signaling, FLT3-ITD in AML
+ PIAS1up-regulates activity img/direct-activation.png sumoylation AKT 0.386
Identifier Residue Sequence Organism Cell Line
SIGNOR-252737 Lys276 NVVYRDLkLENLMLD Mus musculus MEF Cell
pmid sentence
Although multiple sites on Akt could be SUMOylated, K276 was identified as a major SUMO acceptor site. K276R or E278A mutation reduced SUMOylation of Akt but had little effect on its ubiquitination. Strikingly, these mutations also completely abolished Akt kinase activity. In support of these results, we found that expression of PIAS1 and SUMO1 increased Akt activity, whereas expression of SENP1 reduced Akt1 activity.
Publications: 1 Organism: Mus Musculus
+ SENP1down-regulates quantity by destabilization img/direct_inhibition.png desumoylation AKT 0.284
Identifier Residue Sequence Organism Cell Line
SIGNOR-252738 Lys276 NVVYRDLkLENLMLD Mus musculus MEF Cell
pmid sentence
Although multiple sites on Akt could be SUMOylated, K276 was identified as a major SUMO acceptor site. K276R or E278A mutation reduced SUMOylation of Akt but had little effect on its ubiquitination. Strikingly, these mutations also completely abolished Akt kinase activity. In support of these results, we found that expression of PIAS1 and SUMO1 increased Akt activity, whereas expression of SENP1 reduced Akt1 activity.
Publications: 1 Organism: Mus Musculus
+ MUL1down-regulates quantity by destabilization img/direct_inhibition.png ubiquitination AKT 0.487
Identifier Residue Sequence Organism Cell Line
SIGNOR-252460 Lys284 LENLMLDkDGHIKIT Homo sapiens
pmid sentence
The results of the functional studies suggest that the degradation of Akt by MULAN suppresses cell proliferation and viability.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates quantity by stabilization img/direct-activation.png phosphorylation BEX1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-262614 Ser102 KLREKQLsHSLRAVS Rattus norvegicus PC-12 Cell
pmid sentence
Phosphorylation of Bex1 in Ser105 by the serine–threonine kinase AKT stabilizes Bex1 and protects it from proteasomal degradation
Publications: 1 Organism: Rattus Norvegicus
+ AKTup-regulates img/direct-activation.png phosphorylation YBX1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-182493 Ser102 NPRKYLRsVGDGETV Homo sapiens Breast Cancer Cell
pmid sentence
Phosphorylation of yb-1 at the serine 102 residue is required for transcriptional activation of growth-enhancing genes, such as egfr. Herein, we illustrate that activated akt binds to and phosphorylates the yb-1 cold shock domain at ser102
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation ESR1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244243 Ser104 FPPLNSVsPSPLMLL Homo sapiens
pmid sentence
Studies using mutants of er-alpha demonstrated that akt increased estrogen receptor activity through the amino-terminal activation function-1 (af-1). Serines s104 s106, s118, and s167 appear to play a role in the activation of er-alpha by akt.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244247 Ser106 PLNSVSPsPLMLLHP Homo sapiens
pmid sentence
Studies using mutants of er-alpha demonstrated that akt increased estrogen receptor activity through the amino-terminal activation function-1 (af-1). Serines s104 s106, s118, and s167 appear to play a role in the activation of er-alpha by akt.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244255 Ser118 LHPPPQLsPFLQPHG Homo sapiens
pmid sentence
Studies using mutants of er-alpha demonstrated that akt increased estrogen receptor activity through the amino-terminal activation function-1 (af-1). Serines s104 s106, s118, and s167 appear to play a role in the activation of er-alpha by akt.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244251 Ser167 GGRERLAsTNDKGSM Homo sapiens
pmid sentence
Studies using mutants of er-alpha demonstrated that akt increased estrogen receptor activity through the amino-terminal activation function-1 (af-1). Serines s104 s106, s118, and s167 appear to play a role in the activation of er-alpha by akt.
Publications: 4 Organism: Homo Sapiens
Pathways:Luminal Breast Cancer
+ AKTup-regulates activity img/direct-activation.png phosphorylation GRIN2C 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-262620 Ser1081 GSRPRHAsLPSSVAE Mus musculus Cerebellar Granule Cell
pmid sentence
Here, we demonstrate that PKB/Akt directly phosphorylates NR2C on serine 1096 (S1096). In addition, we identify 14-3-3epsilon as an NR2C interactor, whose binding is dependent on S1096 phosphorylation. These data are all consistent with a model in which NR1 and NR2C oligomerize, PKB phosphorylates S1096, and 14-3-3ε binds to phosphorylated NR2C thereby promoting NR2C-containing NMDA receptor surface expression in cerebellar granule cells.
Publications: 1 Organism: Mus Musculus
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation H3-3A 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244275 Ser11 TKQTARKsTGGKAPR Homo sapiens
pmid sentence
Additionally, active akt1 kinase strongly phosphorylates histone h3 at serine 10 in vitro
Publications: 1 Organism: Homo Sapiens
+ AKT img/unknown.png phosphorylation PALLD 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-165497 Ser1118 VRRPRSRsRDSGDEN Homo sapiens
pmid sentence
Akt1, but not akt2, phosphorylates palladin at ser507 in a domain that is critical for f-actin bundling.
Publications: 1 Organism: Homo Sapiens
+ AKT img/unknown.png phosphorylation FANCA 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-251476 Ser1149 CLRSRDPsLMVDFIL in vitro
pmid sentence
FANCA is phosphorylated at Ser1149 by Akt. The biological significance of FANCA phosphorylation and its regulation by Akt remains unclear at this time.
Publications: 1 Organism: In Vitro
+ AKTup-regulates activity img/direct-activation.png phosphorylation PEA15 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244326 Ser116 KDIIRQPsEEEIIKL Homo sapiens HEK-293 Cell
pmid sentence
Protein kinase b/akt binds and phosphorylates ped/pea-15, stabilizing its antiapoptotic action.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation NOS3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244322 Ser1177 TSRIRTQsFSLQERQ Homo sapiens
pmid sentence
Recently many investigators have shown that protein phosphorylation of enos by several serine/threonine kinases is a critical control step for no production by endothelial cells. Phosphorylation by amp kinase, akt (or protein kinase b), or protein kinase a on serine 1179 (bovine) or serine 1177 (human) of enos leads to enhanced activity of the enzyme and, thus, augmented production of no.
Publications: 1 Organism: Homo Sapiens
Pathways:VEGF Signaling
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation BAD 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244148 Ser118 GRELRRMsDEFVDSF Homo sapiens Neuron
pmid sentence
Experiments in this study reveal that akt phosphorylates bad both in vitro and in vivo and that akt-mediated phosphorylation of bad effectively blocks bad induced cell death.[...] In addition, these findings implicate a particular phosphorylation site on bad, serine 136, in the suppression of bad-mediated death by akt.[...]The Phosphorylation of bad may lead to the prevention of cell death via a mechanism that involves the selective association of the phosphorylated forms of bad with 14-3-3 protein isoforms. Akt phosphorylates bad in vitro and in vivo we show that growth factor activation of the pi3'k/akt signaling pathway culminates in the phosphorylation of the bcl-2 family member bad, thereby suppressing apoptosis and promoting cell survival. Akt phosphorylates bad in vitro and in vivo erbb-mediated phosphorylation of bad by akt promotes survival by blocking the interaction of this pro-apoptotic molecule with bcl-2 and bcl-x proteins
Identifier Residue Sequence Organism Cell Line
SIGNOR-251469 Ser75 EIRSRHSsYPAGTED Homo sapiens HEK-293 Cell
pmid sentence
Active Akt induced a significant increase in BAD phosphorylation. mutant BAD with alanine substitutions at Ser112 and Ser136 was not phosphorylated by active Akt . phosphorylation of BAD by Akt will preclude its binding to membrane-anchored Bcl-xL, leading to increased cell survival.
Identifier Residue Sequence Organism Cell Line
SIGNOR-251470 Ser99 PFRGRSRsAPPNLWA Homo sapiens HEK-293 Cell
pmid sentence
Active Akt induced a significant increase in BAD phosphorylation. mutant BAD with alanine substitutions at Ser112 and Ser136 was not phosphorylated by active Akt . phosphorylation of BAD by Akt will preclude its binding to membrane-anchored Bcl-xL, leading to increased cell survival.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244144 Ser99 PFRGRSRsAPPNLWA Homo sapiens Neuron
pmid sentence
Experiments in this study reveal that akt phosphorylates bad both in vitro and in vivo and that akt-mediated phosphorylation of bad effectively blocks bad induced cell death.[...] In addition, these findings implicate a particular phosphorylation site on bad, serine 136, in the suppression of bad-mediated death by akt.[...]The Phosphorylation of bad may lead to the prevention of cell death via a mechanism that involves the selective association of the phosphorylated forms of bad with 14-3-3 protein isoforms. Akt phosphorylates bad in vitro and in vivo we show that growth factor activation of the pi3'k/akt signaling pathway culminates in the phosphorylation of the bcl-2 family member bad, thereby suppressing apoptosis and promoting cell survival. Akt phosphorylates bad in vitro and in vivo erbb-mediated phosphorylation of bad by akt promotes survival by blocking the interaction of this pro-apoptotic molecule with bcl-2 and bcl-x proteins
Publications: 4 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, COVID-19 Causal Network, FLT3-ITD in AML, FLT3-ITD signaling, Inhibition of Apoptosis, Malignant Melanoma, SARS-COV APOPTOSIS, VEGF Signaling
+ AKTup-regulates activity img/direct-activation.png phosphorylation CREB1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-251474 Ser119 EILSRRPsYRKILND Homo sapiens
pmid sentence
The nuclear factor CREB stimulates the expression of cellular genes following its protein kinase A-mediated phosphorylation at Ser-133. Ser-133 phosphorylation, in turn, activates target gene expression by promoting recruitment of the co-activator CBP. |When overexpressed in serum-stimulated cells, Akt/PKB potently induced Ser-133 phosphorylation of CREB and promoted recruitment of CBP.
Identifier Residue Sequence Organism Cell Line
SIGNOR-247992 Ser119 EILSRRPsYRKILND Homo sapiens HEK-293 Cell
pmid sentence
When overexpressed in serum-stimulated cells, Akt/PKB potently induced Ser-133 phosphorylation of CREB and promoted recruitment of CBP. Correspondingly, Akt/PKB stimulated target gene expression via CREB in a phospho(Ser-133)-dependent manner.
Publications: 2 Organism: Homo Sapiens
Pathways:AMPK Signaling, COVID-19 Causal Network, FLT3-ITD signaling, Glioblastoma Multiforme, Inhibition of Apoptosis, Malignant Melanoma, PI3K/AKT Signaling, Thyroid Hormone Metabolism
+ AKTup-regulates activity img/direct-activation.png phosphorylation DOCK6 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-275667 Ser1194 GQRSRLAsMLDSDTE
pmid sentence
Akt and PP2A reciprocally regulate the guanine nucleotide exchange factor Dock6 to control axon growth of sensory neurons|At later developmental stages, the abundance of the kinase Akt increased, resulting in the binding of Akt to Dock6 and the phosphorylation of Dock6 at Ser(1194). | In dorsal root ganglion neurons from mice lacking Dock6, reintroduction of Dock6 with a nonphosphorylatable S1194A mutation rescued axon extension but not branch number, whereas reintroduction of Dock6 with a phosphomimetic S1194E mutation resulted in premature branching
Publications: 1
+ AKTup-regulates quantity by stabilization img/direct-activation.png phosphorylation TBC1D7 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-273541 Ser124 GKLPRSPsFPLEPDD Homo sapiens HEK-293 Cell
pmid sentence
 Sequence analysis identified a putative site for both Akt-mediated phosphorylation and 14-3-3 binding at Ser-124, and we found that Akt phosphorylates TBC1D7 at Ser-124. However, this phosphorylation had no effect on the binding of TBC1D7 to TSC1, but stabilized TBC1D7.
Publications: 1 Organism: Homo Sapiens
+ AKT img/unknown.png phosphorylation RANBP3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-160900 Ser126 VKRERTSsLTQFPPS Homo sapiens
pmid sentence
Akt regulates ranbp3 phosphorylation in vitro and in vivo
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation YAP1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-97485 Ser127 PQHVRAHsSPASLQL Homo sapiens
pmid sentence
One protein that associates with 14-3-3 in an akt-dependent manner is shown here to be the yes-associated protein (yap), which is phosphorylated by akt at serine 127, leading to binding to 14-3-3. Akt promotes yap localization to the cytoplasm, resulting in loss from the nucleus where it functions as a coactivator of transcription factors including p73.
Publications: 1 Organism: Homo Sapiens
Pathways:Integrin Signaling
+ CSNK2A1up-regulates img/direct-activation.png phosphorylation AKT 0.379
Identifier Residue Sequence Organism Cell Line
SIGNOR-174691 Ser129 SGSPSDNsGAEEMEV Homo sapiens HEK-293 Cell
pmid sentence
CK2 hyperactivates AKT by phosphorylation at Ser129
Identifier Residue Sequence Organism Cell Line
SIGNOR-244400 Homo sapiens JURKAT Cell
pmid sentence
Akt/pkb ser129 is phosphorylated by ck2 in vitro and in vivo;(4) such a phosphorylation of activated akt/pkb correlates with a further increase in catalytic activity.
Publications: 2 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network
+ AKTdown-regulates img/direct_inhibition.png phosphorylation NR3C1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-236216 Ser134 ANLNRSTsVPENPKS Homo sapiens Acute Lymphoblastic Leukemia Cell
pmid sentence
Akt1 impairs glucocorticoid-induced gene expression by direct phosphorylation of nr3c1 at position s134 and blocking glucocorticoid-induced nr3c1 translocation to the nucleus
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation MST1R 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-118053 Ser1394 VRRPRPLsEPPRPT Homo sapiens
pmid sentence
Akt/pkb phosphorylates ron ser-1394, thus providing a docking site for 14-3-3based on these results, we propose a mechanism based on msp-ron-dependent phosphorylation and 14-3-3 association, whereby the function of alpha6beta4 switches from a mechanical adhesive device into a signaling component, and might be critically involved in human epidermal wound healing
Publications: 1 Organism: Homo Sapiens
+ AKT img/unknown.png phosphorylation CCDC88A 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-140216 Ser1417 INRERQKsLTLTPTR Homo sapiens
pmid sentence
Akt phosphorylates serine at position 1416 in girdin, and phosphorylated girdin accumulates at the leading edge of migrating cells.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation ZYX 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244389 Ser142 PQPREKVsSIDLEID Homo sapiens
pmid sentence
Akt binds and phosphorylates zyxin on serine 142, leading to its association with acinus zyxin is a substrate of caspases, but akt phosphorylation fails to protect its proteolytic degradation
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation DNMT1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244232 Ser143 RTPRRSKsDGEAKPE Homo sapiens
pmid sentence
Akt1 kinase colocalizes and directly interacts with dnmt1 and phosphorylates ser143. Phosphorylated dnmt1 peaks during dna synthesis, before dnmt1 methylation. Depletion of akt1 or overexpression of dominant-negative akt1 increases methylated dnmt1, resulting in a decrease in dnmt1 abundance. In mammalian cells, phosphorylated dnmt1 is more stable than methylated dnmt1.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation MXD1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-186130 Ser145 IERIRMDsIGSTVSS Homo sapiens
pmid sentence
Here, we present evidence that akt inhibits mad1-mediated transcription repression by physical interaction with and phosphorylation of mad1.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation NCOR1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244318 Ser1450 TVRSRHTsVVSSGPS Homo sapiens Leukemia Cell
pmid sentence
Akt-induced phosphorylation of n-cor at serine 1450 contributes to its misfolded conformational dependent loss (mcdl) in acute myeloid leukemia of the m5 subtype.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation GAB2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-113669 Ser159 LLRERKSsAPSHSSQ Homo sapiens
pmid sentence
Pkb constitutively associates with gab2, phosphorylates gab2 on a consensus phosphorylation site, ser159, in vitro and inhibits gab2 tyrosine phosphorylation.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation ARHGAP22 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-262613 Ser16 ARRARSKsLVMGEQS Homo sapiens HEK-293 Cell
pmid sentence
Akt phosphorylates RhoGAP22 at the 14-3-3 binding site and is required for insulin-stimulated 14-3-3 binding. we have demonstrated that Akt is the kinase responsible for phosphorylation of Ser16 in order to mediate 14-3-3 binding to RhoGAP22.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation EDC3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-262631 Ser161 SFRRRHNsWSSSSRH Cricetulus griseus CHO Cell
pmid sentence
Together these data show that recombinant EDC3 co-immunoprecipitates with endogenous 14-3-3 isoforms in response to insulin in vivo and is phosphorylated at the putative 14-3-3 binding and AKT phosphorylation site Ser-161. Collectively, these data suggest that Ser-161 in EDC3 is phosphorylated in response to insulin principally by AKT and that this subsequently triggers 14-3-3 binding. Constitutive 14-3-3 binding to EDC3 alters p-body morphology and function
Publications: 1 Organism: Cricetulus Griseus
+ AKTup-regulates activity img/direct-activation.png phosphorylation MDM2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244296 Ser166 SSRRRAIsETEENSD Homo sapiens
pmid sentence
Stabilization of mdm2 via decreased ubiquitination is mediated by protein kinase b/akt-dependent phosphorylation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244292 Ser186 RQRKRHKsDSISLSF Homo sapiens
pmid sentence
Mitogen-induced activation of phosphatidylinositol 3-kinase (pi3-kinase) and its downstream target, the akt/pkb serine-threonine kinase, results in phosphorylation of mdm2 on serine 166 and serine 186. Phosphorylation on these sites is necessary for translocation of mdm2 from the cytoplasm into the nucleus.Both akt expression and serum treatment induced phosphorylation of mdm2 at ser186.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244300 Ser188 RKRHKSDsISLSFDE Homo sapiens
pmid sentence
Stabilization of mdm2 via decreased ubiquitination is mediated by protein kinase b/akt-dependent phosphorylationhere we show that pkb inhibits mdm2 self-ubiquitination via phosphorylation of mdm2 on ser(166) and ser(188)
Publications: 3 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, miRNA in AML, FLT3-ITD signaling, Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, Luminal Breast Cancer, Malignant Melanoma, Triple mutant AML, NPM1_new, Prostate Cancer, Pancreatic ductal adenocarcinoma (PDA)
+ AKTup-regulates img/direct-activation.png phosphorylation EP300 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244236 Ser1834 MLRRRMAsMQRTGVV Homo sapiens
pmid sentence
We find that suberoylanilide hydroxamic acid stimulates akt activity, which is required to phosphorylate p300 at ser(1834). Akt-mediated phosphorylation of p300 dramatically increases its acetyltransferase activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-244239 Homo sapiens
pmid sentence
Akt1 and 2 promote the association of myod with p300 and pcaf acetyltransferases, via direct phosphorylation of p300.
Publications: 2 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, FLT3 in AML, COVID-19 Causal Network
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation BAX 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-209651 Ser184 VAGVLTAsLTIWKKM Homo sapiens Polymorphonuclear Neutrophil
pmid sentence
Phosphorylation of Bax Ser184 by Akt regulates its activity and apoptosis in neutrophilsWe suggest that Bax is regulated by phosphorylation of Ser(184) in an Akt-dependent manner and that phosphorylation inhibits Bax effects on the mitochondria by maintaining the protein in the cytoplasm, heterodimerized with antiapoptotic Bcl-2 family members
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, COVID-19 Causal Network, FLT3-ITD signaling, Inhibition of Apoptosis, Malignant Melanoma, SARS-COV APOPTOSIS
+ AKT img/unknown.png phosphorylation HMOX1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-161283 Ser188 LYRSRMNsLEMTPAV Homo sapiens HEK-293 Cell
pmid sentence
We have identified a putative consensus sequence for phosphorylation by akt/pkb of ho-1 at ser188. although the changes in activity are small, this study provides the first evidence for a role of the survival kinase akt in the regulation of ho-1.
Publications: 1 Organism: Homo Sapiens
Pathways:Hepatocellular Tumor
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation KHSRP 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-151216 Ser193 GLPERSVsLTGAPES Homo sapiens
pmid sentence
Beta-catenin transcript can be stabilized by either wnt or pi3k-akt signaling activation. Akt phosphorylates ksrp at a unique serine residue akt phosphorylates the mrna decay-promoting factor ksrp at a unique serine residue, induces its association with the multifunctional protein 14-3-3, and prevents ksrp interaction with the exoribonucleolytic complex exosome.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation CASP9 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-251473 Ser196 KLRRRFSsLHFMVEV in vitro
pmid sentence
Akt phosphorylated recombinant Casp9 in vitro on serine-196 and inhibited its protease activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-71480 Ser196 KLRRRFSsLHFMVEV Homo sapiens Neuron
pmid sentence
Akt phosphorylation site found in human caspase-9 is absent in mouse caspase-9BAD phosphorylation by Akt is an essential step for growth factor-mediated inhibition of caspase activation
Publications: 2 Organism: In Vitro, Homo Sapiens
Pathways:COVID-19 Causal Network, Inhibition of Apoptosis, SARS-COV APOPTOSIS
+ AKTdown-regulates img/direct_inhibition.png phosphorylation, relocalization FOXO 0.908
Identifier Residue Sequence Organism Cell Line
SIGNOR-252828 Ser197 APRRRAAsMDSSSKL Homo sapiens
pmid sentence
Foxo4 transcription factor, also referred to afx, contains three putative phosphorylation motif sites for protein kinase b (pkb), thr32, ser197, and ser262, and it is proposed that phosphorylated foxo4 stays in the cytosol and is imported to the nucleus through dephosphorylation to induce target gene expression
Identifier Residue Sequence Organism Cell Line
SIGNOR-252831 Ser256 SPRRRAAsMDNNSKF in vitro CV-1 Cell
pmid sentence
Here we show that the activation of phosphatidylinositol 3 (PI3) kinase by extracellular growth factors induces phosphorylation, nuclear export, and transcriptional inactivation of FKHR1, a member of the FKHR subclass of the forkhead family of transcription factors. Protein kinase B (PKB)/Akt, a key mediator of PI3 kinase signal transduction, phosphorylated recombinant FKHR1 in vitro at threonine-24 and serine-253. Mutants FKHR1(T24A), FKHR1(S253A), and FKHR1(T24A/S253A) were resistant to both PKB/Akt-mediated phosphorylation and PI3 kinase-stimulated nuclear export.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252829 Ser262 TFRPRSSsNASSVST Homo sapiens
pmid sentence
Foxo4 transcription factor, also referred to afx, contains three putative phosphorylation motif sites for protein kinase b (pkb), thr32, ser197, and ser262, and it is proposed that phosphorylated foxo4 stays in the cytosol and is imported to the nucleus through dephosphorylation to induce target gene expression
Identifier Residue Sequence Organism Cell Line
SIGNOR-252835 Ser319 TFRPRTSsNASTISG Homo sapiens
pmid sentence
The transcription factor, forkhead in rhabdomyosarcoma (fkhr), is phosphorylated at three amino acid residues (thr-24, ser-256 and ser-319) by protein kinase b (pkb)alpha.Fkhr (forkhead in rhabdomyosarcoma), afx (all1 fused gene from chromosome x) and fkhrl1 (fkhr-like 1) are phosphorylated directly by pkb in cells, preventing them from stimulating gene transcription and leading to their exit from the nucleus
Identifier Residue Sequence Organism Cell Line
SIGNOR-252832 Thr24 LPRPRSCtWPLPRPE in vitro CV-1 Cell
pmid sentence
Here we show that the activation of phosphatidylinositol 3 (PI3) kinase by extracellular growth factors induces phosphorylation, nuclear export, and transcriptional inactivation of FKHR1, a member of the FKHR subclass of the forkhead family of transcription factors. Protein kinase B (PKB)/Akt, a key mediator of PI3 kinase signal transduction, phosphorylated recombinant FKHR1 in vitro at threonine-24 and serine-253. Mutants FKHR1(T24A), FKHR1(S253A), and FKHR1(T24A/S253A) were resistant to both PKB/Akt-mediated phosphorylation and PI3 kinase-stimulated nuclear export.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252830 Thr32 QSRPRSCtWPLPRPE Homo sapiens
pmid sentence
Foxo4 transcription factor, also referred to afx, contains three putative phosphorylation motif sites for protein kinase b (pkb), thr32, ser197, and ser262, and it is proposed that phosphorylated foxo4 stays in the cytosol and is imported to the nucleus through dephosphorylation to induce target gene expression
Identifier Residue Sequence Organism Cell Line
SIGNOR-252820 Homo sapiens
pmid sentence
Akt inactivates protein degradation by phosphorylating and thus repressing the transcription factors of the foxo family, and stimulates protein synthesis via the mammalian target of rapamycin (mtor) and glycogen synthase kinase 3b (gsk3b).
Identifier Residue Sequence Organism Cell Line
SIGNOR-252834 Homo sapiens
pmid sentence
The phosphorylation of the two remaining akt-dependent sites inhibits foxo6 transcriptional activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-252837 Mus musculus MEF Cell
pmid sentence
Akt1/PKBalpha was found to be the major regulator of phosphorylation and nuclear export of FoxO1, whose presence in the nucleus strongly attenuates adipocyte differentiation
Identifier Residue Sequence Organism Cell Line
SIGNOR-252824 Homo sapiens
pmid sentence
Akt phosphorylates members of the foxo factors (forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localisation. In particular, akt phosphorylates foxo1 on thr24, ser256 and ser319. Foxo 3alfa and foxo4 are phosphorylated on equivalent sites. In addition, phosphorylation of afx by protein kinase b inhibits its transcriptional activity.
Publications: 10 Organism: Homo Sapiens, In Vitro, Mus Musculus
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, FLT3-ITD in AML, FLT3-ITD signaling, NPM1_new, PI3K/AKT Signaling, Thyroid Hormone Metabolism
+ AKTdown-regulates img/direct_inhibition.png phosphorylation FOXO4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-141416 Ser197 APRRRAAsMDSSSKL Homo sapiens
pmid sentence
Foxo4 transcription factor, also referred to afx, contains three putative phosphorylation motif sites for protein kinase b (pkb), thr32, ser197, and ser262, and it is proposed that phosphorylated foxo4 stays in the cytosol and is imported to the nucleus through dephosphorylation to induce target gene expression
Identifier Residue Sequence Organism Cell Line
SIGNOR-141420 Ser262 TFRPRSSsNASSVST Homo sapiens
pmid sentence
Foxo4 transcription factor, also referred to afx, contains three putative phosphorylation motif sites for protein kinase b (pkb), thr32, ser197, and ser262, and it is proposed that phosphorylated foxo4 stays in the cytosol and is imported to the nucleus through dephosphorylation to induce target gene expression
Identifier Residue Sequence Organism Cell Line
SIGNOR-141424 Thr32 QSRPRSCtWPLPRPE Homo sapiens
pmid sentence
Foxo4 transcription factor, also referred to afx, contains three putative phosphorylation motif sites for protein kinase b (pkb), thr32, ser197, and ser262, and it is proposed that phosphorylated foxo4 stays in the cytosol and is imported to the nucleus through dephosphorylation to induce target gene expression
Identifier Residue Sequence Organism Cell Line
SIGNOR-174021 Homo sapiens
pmid sentence
Akt phosphorylates members of the foxo factors (forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localisation. In particular, akt phosphorylates foxo1 on thr24, ser256 and ser319. Foxo 3alfa and foxo4 are phosphorylated on equivalent sites. In addition, phosphorylation of afx by protein kinase b inhibits its transcriptional activity.
Publications: 4 Organism: Homo Sapiens
Pathways:Insulin Signaling
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation FOXO4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-251477 Ser197 APRRRAAsMDSSSKL Mus musculus NIH-3T3-A14 Cell
pmid sentence
Here we show that protein kinase B phosphorylates AFX, a human orthologue of daf -16 (refs 5, 6, 9), both in vitro and in vivo.
Identifier Residue Sequence Organism Cell Line
SIGNOR-251478 Ser262 TFRPRSSsNASSVST Mus musculus
pmid sentence
Here we show that protein kinase B phosphorylates AFX, a human orthologue of daf -16 (refs 5, 6, 9), both in vitro and in vivo.
Publications: 2 Organism: Mus Musculus
Pathways:Insulin Signaling
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation FOXO 0.908
Identifier Residue Sequence Organism Cell Line
SIGNOR-252838 Ser197 APRRRAAsMDSSSKL Mus musculus NIH-3T3-A14 Cell
pmid sentence
Here we show that protein kinase B phosphorylates AFX, a human orthologue of daf -16 (refs 5, 6, 9), both in vitro and in vivo.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252826 Ser253 APRRRAVsMDNSNKY Homo sapiens Breast Cancer Cell, Prostate Gland Cancer Cell, Leukemia Cell, Glioblastoma Cell
pmid sentence
Akt-dependent phosphorylation of foxo3a (thr32, ser253, and ser315 for human foxo3) enhances foxo3a/14?3?3 Interaction and promotes foxo3a nuclear export to the cytoplasm, resulting in the repression of foxo3a transcriptional function
Identifier Residue Sequence Organism Cell Line
SIGNOR-252839 Ser262 TFRPRSSsNASSVST Mus musculus
pmid sentence
Here we show that protein kinase B phosphorylates AFX, a human orthologue of daf -16 (refs 5, 6, 9), both in vitro and in vivo.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252827 Ser315 DFRSRTNsNASTVSG Homo sapiens Breast Cancer Cell, Prostate Gland Cancer Cell, Leukemia Cell, Glioblastoma Cell
pmid sentence
Akt-dependent phosphorylation of foxo3a (thr32, ser253, and ser315 for human foxo3) enhances foxo3a/14?3?3 Interaction and promotes foxo3a nuclear export to the cytoplasm, resulting in the repression of foxo3a transcriptional function
Identifier Residue Sequence Organism Cell Line
SIGNOR-252825 Thr32 QSRPRSCtWPLQRPE Homo sapiens Breast Cancer Cell, Prostate Gland Cancer Cell, Leukemia Cell, Glioblastoma Cell
pmid sentence
Akt-dependent phosphorylation of foxo3a (thr32, ser253, and ser315 for human foxo3) enhances foxo3a/14-3-3 Interaction and promotes foxo3a nuclear export to the cytoplasm, resulting in the repression of foxo3a transcriptional function
Identifier Residue Sequence Organism Cell Line
SIGNOR-252836 Mus musculus MEF Cell
pmid sentence
Akt1/PKBalpha was found to be the major regulator of phosphorylation and nuclear export of FoxO1, whose presence in the nucleus strongly attenuates adipocyte differentiation
Publications: 6 Organism: Mus Musculus, Homo Sapiens
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, FLT3-ITD in AML, FLT3-ITD signaling, NPM1_new, PI3K/AKT Signaling, Thyroid Hormone Metabolism
+ AKTdown-regulates img/direct_inhibition.png phosphorylation HNRNPA1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-179059 Ser199 SQRGRSGsGNFGGGR Homo sapiens
pmid sentence
Our data also suggest that akt negatively regulates hnrnp a1-mediated ires activity via phosphorylation at ser199.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation EZH2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244259 Ser21 CWRKRVKsEYMRLRQ Homo sapiens
pmid sentence
Enhancer of zeste homolog 2 (ezh2) is a methyltransferase that plays an important role in many biological processes through its ability to trimethylate lysine 27 in histone h3. Here, we show that akt phosphorylates ezh2 at serine 21 and suppresses its methyltransferase activity by impeding ezh2 binding to histone h3
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, ASXL1 in AML, Prostate Cancer
+ AKTdown-regulates img/direct_inhibition.png phosphorylation GSK3A 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-83217 Ser21 SGRARTSsFAEPGGG Homo sapiens
pmid sentence
In response to insulin, gsk3a inhibited by phosphorylation at ser-21 by pkb/akt1;phosphorylation at this site causes a conformational change, preventing access of substrates to the active site.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation HTRA2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-153323 Ser212 RVRVRLLsGDTYEAV Homo sapiens
pmid sentence
Akt attenuation of the serine protease activity of htra2/omi through phosphorylation of serine 212
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation AR 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244140 Ser215 SGRAREAsGAPTSSK Homo sapiens Neuron
pmid sentence
The work presented here is the first demonstration that phosphorylation at s215 and s792 by akt regulates ligand binding, and the subcellular distribution of the receptor
Publications: 1 Organism: Homo Sapiens
Pathways:Prostate Cancer
+ AKTup-regulates quantity by stabilization img/direct-activation.png phosphorylation FLNC 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-262616 Ser2233 LGRERLGsFGSITRQ Mus musculus C2C12 Cell
pmid sentence
We identified the extended basophilic phosphosite motif RxRxxp[S/T]xxp[S/T] in various proteins including filamin-C (FLNc). Importantly, this extended motif, located in a unique insert in Ig-like domain 20 of FLNc, is doubly phosphorylated. The protein kinases responsible for this dual-site phosphorylation are Akt and PKCα. Proximity proteomics and interaction analysis identified filamin A-interacting protein 1 (FILIP1) as direct FLNc binding partner. FILIP1 binding induces filamin degradation, thereby negatively regulating its function. Here, dual-site phosphorylation of FLNc not only reduces FILIP1 binding, providing a mechanism to shield FLNc from FILIP1-mediated degradation, but also enables fast dynamics of FLNc necessary for its function as signaling adaptor in cross-striated muscle cells. In vitro kinase assays combined with LC-MS confirmed hFLNc-S2233 as a substrate of Akt, whereas PKCα preferentially targeted S2236.
Publications: 1 Organism: Mus Musculus
+ AKTup-regulates activity img/direct-activation.png phosphorylation ZNF322 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-276755 Ser224 EKSYRHRsAFIVHKR Homo sapiens NCI-H1299 Cell
pmid sentence
We studied AKT-mediated phosphorylation sites, viz. Thr-150, Ser-224, Thr-234, and Thr-262. ZNF322A phosphorylation at Thr-262 by AKT promoted ZNF322A protein stability thus increased ADD1 promoter activity. Interestingly, phosphorylation at Thr-150, Ser-224, and Thr-234 enhanced transcription activity without affecting protein stability of ZNF322A.
Identifier Residue Sequence Organism Cell Line
SIGNOR-276754 Thr150 LVHQRSHtGEKPYLC Homo sapiens NCI-H1299 Cell
pmid sentence
We studied AKT-mediated phosphorylation sites, viz. Thr-150, Ser-224, Thr-234, and Thr-262. ZNF322A phosphorylation at Thr-262 by AKT promoted ZNF322A protein stability thus increased ADD1 promoter activity. Interestingly, phosphorylation at Thr-150, Ser-224, and Thr-234 enhanced transcription activity without affecting protein stability of ZNF322A.
Identifier Residue Sequence Organism Cell Line
SIGNOR-276752 Thr234 IVHKRVHtGEKPYKC Homo sapiens NCI-H1299 Cell
pmid sentence
We studied AKT-mediated phosphorylation sites, viz. Thr-150, Ser-224, Thr-234, and Thr-262. ZNF322A phosphorylation at Thr-262 by AKT promoted ZNF322A protein stability thus increased ADD1 promoter activity. Interestingly, phosphorylation at Thr-150, Ser-224, and Thr-234 enhanced transcription activity without affecting protein stability of ZNF322A.
Identifier Residue Sequence Organism Cell Line
SIGNOR-276753 Thr262 IVHQRVHtGEKPYKC Homo sapiens NCI-H1299 Cell
pmid sentence
We studied AKT-mediated phosphorylation sites, viz. Thr-150, Ser-224, Thr-234, and Thr-262. ZNF322A phosphorylation at Thr-262 by AKT promoted ZNF322A protein stability thus increased ADD1 promoter activity. Interestingly, phosphorylation at Thr-150, Ser-224, and Thr-234 enhanced transcription activity without affecting protein stability of ZNF322A.
Publications: 4 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation TERT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244361 Ser227 GARRRGGsASRSLPL Homo sapiens
pmid sentence
Akt kinase enhances human telomerase activity through phosphorylation of htert subunit as one of its substrate proteins.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244357 Ser824 AVRIRGKsYVQCQGI Homo sapiens
pmid sentence
Akt kinase enhances human telomerase activity through phosphorylation of htert subunit as one of its substrate proteins.
Publications: 2 Organism: Homo Sapiens
Pathways:Hepatocellular Tumor, Pancreatic ductal adenocarcinoma (PDA)
+ AKT img/unknown.png phosphorylation MTOR 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244311 Ser2448 RSRTRTDsYSAGQSV Homo sapiens
pmid sentence
AKT phosphorylated mTOR at two COOH-terminal sites (Thr2446 and Ser2448) in vitro, Ser2448 was the major phosphorylation site in insulin-stimulated or -activated AKT-expressing human embryonic kidney cells. These results demonstrate that mTOR is a direct target of the PI3K-AKT signaling pathway in mitogen-stimulated cells, and that the identified AKT phosphorylation sites are nested within a repressor domain that negatively regulates the catalytic activity of mTOR. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-251482 Thr2446 NKRSRTRtDSYSAGQ Homo sapiens
pmid sentence
AKT phosphorylated mTOR at two COOH-terminal sites (Thr2446 and Ser2448) in vitro, Ser2448 was the major phosphorylation site in insulin-stimulated or -activated AKT-expressing human embryonic kidney cells. These results demonstrate that mTOR is a direct target of the PI3K-AKT signaling pathway in mitogen-stimulated cells, and that the identified AKT phosphorylation sites are nested within a repressor domain that negatively regulates the catalytic activity of mTOR. 
Publications: 2 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, KIT in AML, miRNA in AML, FLT3-ITD in AML, FLT3-ITD signaling, Glioblastoma Multiforme, Leptin Signaling, MTOR Signaling, Pancreatic ductal adenocarcinoma (PDA)
+ AKTup-regulates img/direct-activation.png phosphorylation TP53RK 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-157467 Ser250 RLRGRKRsMVG Homo sapiens
pmid sentence
Here we show that such an activation of prpk is mediated by another kinase, akt/pkb, which phosphorylates prpk at ser250.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation FOXO3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-183612 Ser253 APRRRAVsMDNSNKY Homo sapiens Breast Cancer Cell, Prostate Gland Cancer Cell, Leukemia Cell, Glioblastoma Cell
pmid sentence
Akt-dependent phosphorylation of foxo3a (thr32, ser253, and ser315 for human foxo3) enhances foxo3a/14?3?3 Interaction and promotes foxo3a nuclear export to the cytoplasm, resulting in the repression of foxo3a transcriptional function
Identifier Residue Sequence Organism Cell Line
SIGNOR-183616 Ser315 DFRSRTNsNASTVSG Homo sapiens Breast Cancer Cell, Prostate Gland Cancer Cell, Leukemia Cell, Glioblastoma Cell
pmid sentence
Akt-dependent phosphorylation of foxo3a (thr32, ser253, and ser315 for human foxo3) enhances foxo3a/14?3?3 Interaction and promotes foxo3a nuclear export to the cytoplasm, resulting in the repression of foxo3a transcriptional function
Identifier Residue Sequence Organism Cell Line
SIGNOR-183620 Thr32 QSRPRSCtWPLQRPE Homo sapiens Breast Cancer Cell, Prostate Gland Cancer Cell, Leukemia Cell, Glioblastoma Cell
pmid sentence
Akt-dependent phosphorylation of foxo3a (thr32, ser253, and ser315 for human foxo3) enhances foxo3a/14-3-3 Interaction and promotes foxo3a nuclear export to the cytoplasm, resulting in the repression of foxo3a transcriptional function
Identifier Residue Sequence Organism Cell Line
SIGNOR-261526 Mus musculus
pmid sentence
Phosphorylation of Foxo proteins through FLT3-ITD signaling promotes their translocation from the nucleus into the cytoplasm, which requires the presence of conserved Akt phosphorylation sites in Forkhead transcription factors and PI3K activity.
Publications: 4 Organism: Homo Sapiens, Mus Musculus
Pathways:FLT3 in AML, AMPK Signaling, Insulin Signaling, Inhibition of Apoptosis, Integrin Signaling, Thyroid cancer
+ AKTdown-regulates quantity by destabilization img/direct_inhibition.png phosphorylation FOXO3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-249646 Ser253 APRRRAVsMDNSNKY
pmid sentence
AKT phosphorylates FOXO3a at three conserved sites (Thr32, Ser253 and Ser315), therefore creating binding sites for the 14-3-3 chaperone proteins and leading to the active export of FOXO3a to the cytoplasm where it is targeted for proteasomal degradation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249647 Ser315 DFRSRTNsNASTVSG
pmid sentence
AKT phosphorylates FOXO3a at three conserved sites (Thr32, Ser253 and Ser315), therefore creating binding sites for the 14-3-3 chaperone proteins and leading to the active export of FOXO3a to the cytoplasm where it is targeted for proteasomal degradation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249645 Thr32 QSRPRSCtWPLQRPE
pmid sentence
AKT phosphorylates FOXO3a at three conserved sites (Thr32, Ser253 and Ser315), therefore creating binding sites for the 14-3-3 chaperone proteins and leading to the active export of FOXO3a to the cytoplasm where it is targeted for proteasomal degradation.
Publications: 3
Pathways:FLT3 in AML, AMPK Signaling, Insulin Signaling, Inhibition of Apoptosis, Integrin Signaling, Thyroid cancer
+ AKTdown-regulates quantity by destabilization img/direct_inhibition.png phosphorylation FOXO 0.908
Identifier Residue Sequence Organism Cell Line
SIGNOR-252821 Ser253 APRRRAVsMDNSNKY Homo sapiens
pmid sentence
AKT phosphorylates FOXO3a at three conserved sites (Thr32, Ser253 and Ser315), therefore creating binding sites for the 14-3-3 chaperone proteins and leading to the active export of FOXO3a to the cytoplasm where it is targeted for proteasomal degradation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252822 Ser315 DFRSRTNsNASTVSG Homo sapiens
pmid sentence
AKT phosphorylates FOXO3a at three conserved sites (Thr32, Ser253 and Ser315), therefore creating binding sites for the 14-3-3 chaperone proteins and leading to the active export of FOXO3a to the cytoplasm where it is targeted for proteasomal degradation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252823 Thr32 QSRPRSCtWPLQRPE Homo sapiens
pmid sentence
AKT phosphorylates FOXO3a at three conserved sites (Thr32, Ser253 and Ser315), therefore creating binding sites for the 14-3-3 chaperone proteins and leading to the active export of FOXO3a to the cytoplasm where it is targeted for proteasomal degradation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252833 Homo sapiens
pmid sentence
Phosphorylation of FoxOs by Akt inhibits transcriptional functions of FoxOs and contributes to cell survival, growth and proliferation.The PI3K/Akt signaling regulates cell proliferation and survival in part by phosphorylating FoxOs to promote their interaction with 14-3-3 protein that results in nuclear exclusion and eventual ubiquitin proteasome pathway (UPP)-dependent degradation of FoxOs
Publications: 4 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, FLT3-ITD in AML, FLT3-ITD signaling, NPM1_new, PI3K/AKT Signaling, Thyroid Hormone Metabolism
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation FOXO1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-252346 Ser256 SPRRRAAsMDNNSKF in vitro CV-1 Cell
pmid sentence
Here we show that the activation of phosphatidylinositol 3 (PI3) kinase by extracellular growth factors induces phosphorylation, nuclear export, and transcriptional inactivation of FKHR1, a member of the FKHR subclass of the forkhead family of transcription factors. Protein kinase B (PKB)/Akt, a key mediator of PI3 kinase signal transduction, phosphorylated recombinant FKHR1 in vitro at threonine-24 and serine-253. Mutants FKHR1(T24A), FKHR1(S253A), and FKHR1(T24A/S253A) were resistant to both PKB/Akt-mediated phosphorylation and PI3 kinase-stimulated nuclear export. These results indicate that phosphorylation by PKB/Akt negatively regulates FKHR1 by promoting export from the nucleus.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252349 Ser319 TFRPRTSsNASTISG Homo sapiens
pmid sentence
The transcription factor, forkhead in rhabdomyosarcoma (fkhr), is phosphorylated at three amino acid residues (thr-24, ser-256 and ser-319) by protein kinase b (pkb)alpha.Fkhr (forkhead in rhabdomyosarcoma), afx (all1 fused gene from chromosome x) and fkhrl1 (fkhr-like 1) are phosphorylated directly by pkb in cells, preventing them from stimulating gene transcription and leading to their exit from the nucleus
Identifier Residue Sequence Organism Cell Line
SIGNOR-252347 Thr24 LPRPRSCtWPLPRPE in vitro CV-1 Cell
pmid sentence
Here we show that the activation of phosphatidylinositol 3 (PI3) kinase by extracellular growth factors induces phosphorylation, nuclear export, and transcriptional inactivation of FKHR1, a member of the FKHR subclass of the forkhead family of transcription factors. Protein kinase B (PKB)/Akt, a key mediator of PI3 kinase signal transduction, phosphorylated recombinant FKHR1 in vitro at threonine-24 and serine-253. Mutants FKHR1(T24A), FKHR1(S253A), and FKHR1(T24A/S253A) were resistant to both PKB/Akt-mediated phosphorylation and PI3 kinase-stimulated nuclear export.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252350 Mus musculus MEF Cell
pmid sentence
Akt1/PKBalpha was found to be the major regulator of phosphorylation and nuclear export of FoxO1, whose presence in the nucleus strongly attenuates adipocyte differentiation
Identifier Residue Sequence Organism Cell Line
SIGNOR-252352 Homo sapiens
pmid sentence
Akt inactivates protein degradation by phosphorylating and thus repressing the transcription factors of the foxo family, and stimulates protein synthesis via the mammalian target of rapamycin (mtor) and glycogen synthase kinase 3b (gsk3b).
Identifier Residue Sequence Organism Cell Line
SIGNOR-252348 Homo sapiens
pmid sentence
Phosphorylation of FoxOs by Akt inhibits transcriptional functions of FoxOs and contributes to cell survival, growth and proliferation.The PI3K/Akt signaling regulates cell proliferation and survival in part by phosphorylating FoxOs to promote their interaction with 14-3-3 protein that results in nuclear exclusion and eventual ubiquitin proteasome pathway (UPP)-dependent degradation of FoxOs
Identifier Residue Sequence Organism Cell Line
SIGNOR-277740 Homo sapiens PANC-1 Cell
pmid sentence
The results showed that TNC induced the cytoplasmic translocation of pFOXO1 via regulation of AKT activation. As shown in Fig. 3C, stimulation of PANC-1 cells with exogenous TNC markedly increased the phosphorylation of AKT and FOXO1. Our study showed that pFOXO1 translocates from the nucleus to the cell cytoplasm after exogenous TNC treatment, which indicates that its transcriptional activity was inhibited.
Publications: 7 Organism: In Vitro, Homo Sapiens, Mus Musculus
Pathways:FAP: Insulin-mediated adipogenesis, Insulin Signaling, Inhibition of Apoptosis, Leptin Signaling, Rhabdomyosarcoma, WNT/FLT3
+ AKTdown-regulates img/direct_inhibition.png phosphorylation RAF1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244337 Ser259 SQRQRSTsTPNVHMV Homo sapiens Breast Cancer Cell, Prostate Gland Cancer Cell
pmid sentence
Akt and protein kinase a (pka) phosphorylate s259 on raf-1 and inhibit its activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-72669 Ser259 SQRQRSTsTPNVHMV Homo sapiens Myoblast
pmid sentence
The stage-specific inhibitory action of Akt correlated with its stage-specific ability to form a complex with Raf, suggesting the existence of differentially expressed mediators of an inhibitory Akt-Raf complex.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244333 Homo sapiens
pmid sentence
Akt negatively regulates the raf and gsk-3 kinases and the cell cycle regulatory transcription factor fkhr.
Publications: 3 Organism: Homo Sapiens
Tissue: Muscle, Myotube
Pathways:COVID-19 Causal Network, Insulin Signaling, Integrin Signaling
+ AKT img/unknown.png phosphorylation CCT2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244172 Ser260 GSRVRVDsTAKVAEI Homo sapiens
pmid sentence
Furthermore, ha-tagged akt can phosphorylate gst-cct_ protein in vitro
Publications: 1 Organism: Homo Sapiens
+ AKT img/unknown.png phosphorylation ARFIP2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-135105 Ser260 GTRGRLEsAQATFQA Homo sapiens Neuron
pmid sentence
Akt phosphorylated arfaptin 2 at ser(260). we have also demonstrated that arfaptin 2 phosphorylation restores proteasome activity that is inhibited by the presence of polyq-huntingtin in cells.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation METTL1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244307 Ser27 YYRQRAHsNPMADHT Homo sapiens
pmid sentence
The trna methylase mettl1 is phosphorylated and inactivated by pkb and rsk in vitro and in cells
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates quantity img/direct_inhibition.png phosphorylation CFLAR 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-245304 Ser273 LLRDTFTsLGYEVQK Homo sapiens
pmid sentence
TNFalpha enhanced FLIP(L) serine phosphorylation, which was increased by activated Akt-1. Serine 273, a putative Akt-1 phosphorylation site in FLIP(L), was critical for the activation-induced reduction of FLIP(L). Thus, these observations document a novel mechanism where by TNFalpha facilitates the reduction of FLIP(L) protein, which is dependent on the phosphatidylinositol 3-kinase/Akt signaling.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation CELF1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-262615 Ser28 GQVPRTWsEKDLREL Mus musculus C2C12 Cell
pmid sentence
CUG repeat binding protein, CUGBP1, is a key regulator of translation of proteins that are involved in muscle development and differentiation. In normal myoblasts, Akt kinase phosphorylates CUGBP1 at Ser28 and increases interactions of CUGBP1 with cyclin D1 mRNA.
Publications: 1 Organism: Mus Musculus
+ AKTdown-regulates img/direct_inhibition.png phosphorylation CHEK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244206 Ser280 AKRPRVTsGGVSESP Homo sapiens
pmid sentence
The chk1 protein phosphorylated by pkb on serine 280 does not enter into protein complexes after replication arrest. Moreover, chk1 phosphorylated by pkb fails to undergo activating phosphorylation on serine 345 by atm/atr. Phosphorylation by atm/atr and association with other checkpoint proteins are essential steps in activation of chk1.
Publications: 1 Organism: Homo Sapiens
Pathways:FLT3-ITD signaling, Cell cycle: G2/M phase transition
+ AKTdown-regulates img/direct_inhibition.png phosphorylation CDKN1C 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-201042 Ser282 FFAKRKRsAPEKSSG Homo sapiens Breast Cancer Cell
pmid sentence
Cdk inhibitor p57 (kip2) is downregulated by akt during her2-mediated tumorigenicityakt phosphorylates p57 on ser 282 or thr310. Akt activity results in destabilization of p57 by accelerating turnover rate of p57 and enhancing p57 ubiquitination
Identifier Residue Sequence Organism Cell Line
SIGNOR-201046 Thr310 GVGSVEQtPRKRLR Homo sapiens Breast Cancer Cell
pmid sentence
Cdk inhibitor p57 (kip2) is downregulated by akt during her2-mediated tumorigenicityakt phosphorylates p57 on ser 282 or thr310. Akt activity results in destabilization of p57 by accelerating turnover rate of p57 and enhancing p57 ubiquitination
Publications: 2 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation PHF20 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-196112 Ser291 ELRRRKIsKGCEVPL Homo sapiens
pmid sentence
Akt phosphorylates phf20 at ser(291) in vitro and in vivo, which results in its translocation from the nucleus to the cytoplasm and attenuation of phf20 function.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation PDE3B 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-251483 Ser295 VIRPRRRsSCVSLGE Mus musculus NIH-3T3 Cell
pmid sentence
PDE3B is a physiological substrate of Akt and that Akt-mediated phosphorylation of PDE3B on serine-273 is important for insulin-induced activation of PDE3B
Identifier Residue Sequence Organism Cell Line
SIGNOR-248027 Ser318 CKIFRRPsLPCISRE Mus musculus 3T3-L1 Cell
pmid sentence
PDE3B is a physiological substrate of Akt and that Akt-mediated phosphorylation of PDE3B on serine-273 is important for insulin-induced activation of PDE3B.
Publications: 2 Organism: Mus Musculus
+ AKTup-regulates img/direct-activation.png phosphorylation PDE3B 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-70205 Ser295 VIRPRRRsSCVSLGE Homo sapiens
pmid sentence
Pde3b is a physiological substrate of akt and that akt-mediated phosphorylation of pde3b on serine-273 is important for insulin-induced activation of pde3b.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation SH3RF1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-155229 Ser304 KNTKKRHsFTSLTMA Homo sapiens
pmid sentence
We report here that posh is a direct substrate for phosphorylation by akt in vivo and in vitro, and we identify a major site of akt phosphorylation as serine 304 of posh, which lies within the rac-binding domain. We further show that phosphorylation of posh results in a decreased ability to bind activated rac, as does phosphomimetic s304d and s304e mutation of posh.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation NCF1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-72133 Ser304 GAPPRRSsIRNAHSI Homo sapiens Neutrophil
pmid sentence
Akt phosphorylates p47phox and mediates respiratory burst activity in human neutrophils. A direct interaction between p47(phox) and akt was shown. Active recombinant akt phosphorylated recombinant p47(phox) in vitro. Mutation analysis indicated that 2 aa residues, ser(304) and ser(328), were phosphorylated by akt. Inhibition of akt activity also inhibited fmlp-stimulated neutrophil chemotaxis.
Identifier Residue Sequence Organism Cell Line
SIGNOR-72137 Ser328 QDAYRRNsVRFLQQR Homo sapiens Neutrophil
pmid sentence
Akt phosphorylates p47phox and mediates respiratory burst activity in human neutrophils. A direct interaction between p47(phox) and akt was shown. Active recombinant akt phosphorylated recombinant p47(phox) in vitro. Mutation analysis indicated that 2 aa residues, ser(304) and ser(328), were phosphorylated by akt. Inhibition of akt activity also inhibited fmlp-stimulated neutrophil chemotaxis.
Publications: 2 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation PIKFYVE 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-130920 Ser307 PARNRSAsITNLSLD Homo sapiens
pmid sentence
Here we report that serine318 on the fyve domain-containing ptdins3p 5-kinase (pikfyve) is a novel substrate for pkb, and show that phosphorylation stimulates the ptdins3p 5-kinase activity of the enzyme.
Publications: 1 Organism: Homo Sapiens
Tissue: Muscle
+ AKTup-regulates img/direct-activation.png phosphorylation GATA1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244267 Ser310 QTRNRKAsGKGKKKR Homo sapiens
pmid sentence
We found that akt directly phosphorylates the transcription factor gata-1 at serine 310 and that this site-specific phosphorylation is required for the transcriptional activation of the timp-1 promoter.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation BMI1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-249581 Ser316 ANRPRKSsVNGSSAT Homo sapiens Prostate Cancer Cell Line
pmid sentence
The polycomb group silencing protein Bmi1 can be phosphorylated by AKT, which enhances its oncogenic potential in PCa. Overexpression of Bmi1 can act in combination with PTEN haploinsufficiency to induce invasive carcinogenic formation in the prostate
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation ATG4B 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-275836 Ser34 WILGRKYsIFTEKDE Homo sapiens Colonic Cancer Cell
pmid sentence
In this study, we identified a novel phosphorylation site at Ser34 of ATG4B induced by AKT in HCC cells.| In brief, our results demonstrate for the first time that the phosphorylation of ATG4B at Ser34 participates in the metabolic reprogramming of HCC cells via repressing mitochondrial function, which possibly results from the Ser34 phosphorylation-induced mitochondrial enrichment of ATG4B and the subsequent inhibition of F1Fo-ATP synthase activity.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation CLK2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244214 Ser34 HKRRRSRsWSSSSDR Homo sapiens HeLa Cell
pmid sentence
Akt directly binds to and phosphorylates clk2 on serine 34 and threonine 127, in vitro and in vivo.Our results suggest that akt activation controls cell survival to ionizing radiation by phosphorylating clk2, revealing an important regulatory mechanism required for promoting cell surviva
Identifier Residue Sequence Organism Cell Line
SIGNOR-244218 Thr127 RRRRRSRtFSRSSSQ Homo sapiens HeLa Cell
pmid sentence
Akt directly binds to and phosphorylates clk2 on serine 34 and threonine 127, in vitro and in vivo.Our results suggest that akt activation controls cell survival to ionizing radiation by phosphorylating clk2, revealing an important regulatory mechanism required for promoting cell surviva
Publications: 2 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation ADRB2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-114466 Ser345 ELLCLRRsSLKAYGN Homo sapiens
pmid sentence
Akt mediates sequestration of the beta(2)-adrenergic receptor in response to insulin. Phosphorylation studies of the c-terminal cytoplasmic domain of the beta(2)-adrenergic receptor by akt in vitro identified ser(345) and ser(346) within a consensus motif for akt phosphorylation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-114470 Ser346 LLCLRRSsLKAYGNG Homo sapiens
pmid sentence
Akt mediates sequestration of the beta(2)-adrenergic receptor in response to insulin. Phosphorylation studies of the c-terminal cytoplasmic domain of the beta(2)-adrenergic receptor by akt in vitro identified ser(345) and ser(346) within a consensus motif for akt phosphorylation.
Publications: 2 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation NR4A1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-105927 Ser351 GRRGRLPsKPKQPPD Homo sapiens T-lymphocyte
pmid sentence
We show that akt interacts with nur77 and inactivates nur77 by phosphorylation at ser-350
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation VCP 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-145284 Ser352 AATNRPNsIDPALRR Homo sapiens Breast Cancer Cell
pmid sentence
Site-directed mutagenesis identified ser-351, ser-745, and ser-747 as akt phosphorylation sites on vcp. however, our study also suggests that other known biological activities of vcp, such as those related to intracellular trafficking, ubiquitin-mediated proteolysis, and activation of transcription (28), might be regulated by akt through the activation of vcp. I
Identifier Residue Sequence Organism Cell Line
SIGNOR-145288 Ser746 AMRFARRsVSDNDIR Homo sapiens Breast Cancer Cell
pmid sentence
Site-directed mutagenesis identified ser-351, ser-745, and ser-747 as akt phosphorylation sites on vcp. however, our study also suggests that other known biological activities of vcp, such as those related to intracellular trafficking, ubiquitin-mediated proteolysis, and activation of transcription (28), might be regulated by akt through the activation of vcp. I
Identifier Residue Sequence Organism Cell Line
SIGNOR-145292 Ser748 RFARRSVsDNDIRKY Homo sapiens Breast Cancer Cell
pmid sentence
Site-directed mutagenesis identified ser-351, ser-745, and ser-747 as akt phosphorylation sites on vcp. however, our study also suggests that other known biological activities of vcp, such as those related to intracellular trafficking, ubiquitin-mediated proteolysis, and activation of transcription (28), might be regulated by akt through the activation of vcp. I
Publications: 3 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation BRAF 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244152 Ser364 FGQRDRSsSAPNVHI Homo sapiens
pmid sentence
We show that phosphorylation of b-raf by akt occurs at multiple residues within its amino terminal regulatory domain, at both the conserved and unique phosphorylation sites. Akt phosphorylated b-raf on s364 and s428 to inactivate its kinase activity b-raf contains three akt consensus sites, table i. One site, ser364 is conserved with c-raf;however, two sites, ser428 and thr439, are unique to b-raf
Identifier Residue Sequence Organism Cell Line
SIGNOR-244160 Ser428 GPQRERKsSSSSEDR Homo sapiens
pmid sentence
We show that phosphorylation of b-raf by akt occurs at multiple residues within its amino terminal regulatory domain, at both the conserved and unique phosphorylation sites. Akt phosphorylated b-raf on s364 and s428 to inactivate its kinase activity b-raf contains three akt consensus sites, table i. One site, ser364 is conserved with c-raf;however, two sites, ser428 and thr439, are unique to b-raf
Identifier Residue Sequence Organism Cell Line
SIGNOR-244156 Thr440 EDRNRMKtLGRRDSS Homo sapiens
pmid sentence
We show that phosphorylation of b-raf by akt occurs at multiple residues within its amino terminal regulatory domain, at both the conserved and unique phosphorylation sites. Akt phosphorylated b-raf on s364 and s428 to inactivate its kinase activity b-raf contains three akt consensus sites, table i. One site, ser364 is conserved with c-raf;however, two sites, ser428 and thr439, are unique to b-raf
Publications: 3 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, KIT in AML, AML_TRIPLETS, EGFR Signaling, ErbB receptors in cancer, FLT3-ITD signaling, Glioblastoma Multiforme, Hepatocellular Tumor, Inhibition of Apoptosis, Luminal Breast Cancer, Malignant Melanoma, NPM1_new, Non-small-cell lung cancer (NSCLC), Pancreatic ductal adenocarcinoma (PDA), PI3K/AKT Signaling, Rhabdomyosarcoma, RTKs in cancer, Thyroid cancer, T cell activation, VEGF Signaling
+ AKTup-regulates activity img/direct-activation.png phosphorylation MDM4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-178063 Ser367 PDCRRTIsAPVVRPK Homo sapiens
pmid sentence
We demonstrate that the serine/threonine kinase akt mediates phosphorylation of mdmx at ser367. This phosphorylation leads to stabilization of mdmx and consequent stabilization of mdm2.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation TTC3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-162984 Ser378 AYTPRSLsAPIFTTS Homo sapiens
pmid sentence
Phosphorylation of ttc3 at ser378 is required for efficient biological function together, these observations support that ttc3 is a phosphorylation target of akt both in an in vitro and in a cellular context
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates quantity by stabilization img/direct-activation.png phosphorylation VIM 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-167971 Ser39 TTSTRTYsLGSALRP Homo sapiens
pmid sentence
The binding of akt (tail region) to vim (head region) results in vim ser39 phosphorylation enhancing the ability of vim to induce motility and invasion while protecting vim from caspase-induced proteolysis.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates quantity by stabilization img/direct-activation.png phosphorylation ACOT4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-271821 Ser392 GGEPRAHsKAQEDAW Homo sapiens BxPC-3 Cell
pmid sentence
HSPA1A was found to associate with ACOT4. Furthermore, we found that phosphorylation of ACOT4 at S392 by AKT decreased the binding of ACOT4 to HSPA1A, resulting in ACOT4 accumulation. |AKT phosphorylates ACOT4 at S392 and promotes ACOT4 stability
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation MAP3K8 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-251480 Ser400 EDQPRCQsLDSALLE Homo sapiens HEK-293 Cell
pmid sentence
Akt-dependent phosphorylation of Cot occurs exclusively on serines 400 and 413. Akt to phosphorylate Cot at two sites in the carboxy-terminal domain, at least one of which may promote binding of substrates or coactivators to Cot, or alternatively may relieve binding of a negative regulator.
Identifier Residue Sequence Organism Cell Line
SIGNOR-251481 Ser413 LERKRLLsRKELELP Homo sapiens HEK-293 Cell
pmid sentence
Akt-dependent phosphorylation of Cot occurs exclusively on serines 400 and 413. Akt to phosphorylate Cot at two sites in the carboxy-terminal domain, at least one of which may promote binding of substrates or coactivators to Cot, or alternatively may relieve binding of a negative regulator.
Publications: 2 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation GATA2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244271 Ser401 QTRNRKMsNKSKKSK Homo sapiens Monocyte
pmid sentence
We show that insulin induces gata2 phosphorylation on serine 401 in a pi-3k/akt-dependent manner. Insulin-dependent phosphorylation of serine 401 impairs gata2 translocation to the nucleus and its dna binding activity
Publications: 1 Organism: Homo Sapiens
Pathways:FAP: Insulin-mediated adipogenesis, Thyroid Hormone Metabolism
+ AKT img/unknown.png phosphorylation HTT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-89696 Ser419 GGRSRSGsIVELIAG Homo sapiens Neuron
pmid sentence
We demonstrate that huntingtin is a substrate of akt and that phosphorylation of huntingtin by akt is crucial to mediate the neuroprotective effects of igf-1.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation TWIST1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244373 Ser42 GGRKRRSsRRSAGGG Homo sapiens
pmid sentence
Moreover, phosphorylation of twist-1 at ser42 was shown in vivo in various human cancer tissues, suggesting that this post-translational modification ensures functional activation of twist-1 after promotion of survival during carcinogenesis.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, miRNA in AML
+ AKTup-regulates img/direct-activation.png phosphorylation EIF4B 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-181536 Ser422 RERSRTGsESSQTGT Homo sapiens
pmid sentence
Using an in vitro kinase assay, we found that pkb can directly phosphorylate eif4b on serine 422 (ser422). This was prevented by pretreatment of cells with the phosphatidylinositol 3-kinase (pi3k) inhibitor ly294002 or pharmacological inhibition of pkb. Phosphorylation regultes the activation of eukaryotic translation initiation factor 4b.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation USP14 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-265055 Ser432 THQGRSSsSGHYVSW Homo sapiens HEK-293 Cell
pmid sentence
Phosphorylation and activation of ubiquitin-specific protease-14 by Akt regulates the ubiquitin-proteasome system|These results suggested S432 as a major and S143 as a minor phosphorylation site of Akt.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation KLHL3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-273825 Ser433 PMNTRRSsVGVGVVE in vitro
pmid sentence
Consistent with the fact that S433 is a component of Akt and PKA phosphorylation motifs, in vitro kinase assay demonstrated that Akt and PKA can phosphorylate KLHL3 at S433, that was previously reported to be phosphorylated by PKC. 
Publications: 1 Organism: In Vitro
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation SIK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-277835 Ser435 VFRPRPVsPSSLLDT Homo sapiens HEK-293 Cell
pmid sentence
 Mass spectrometry-based analyses demonstrated that salt-inducible kinase 1 (SIK1) binds AKT and undergoes AKT-mediated phosphorylation, which compromises SIK1 tumor-suppressive functions. 
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation RGCC (isoform 2) 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-262629 Ser45 ERMKRRSsASVSDSS Homo sapiens HeLa Cell
pmid sentence
Akt phosphorylated GST-RGC-32 in vitro, and CDC2 was also able to phosphorylate RGC-32.  Akt failed to phosphorylate RGC-32 S45A-S47A mutant. These data indicate that Ser 45 and Ser 47 may be the RGC-32 phosphorylation sites for Akt kinase.
Identifier Residue Sequence Organism Cell Line
SIGNOR-262628 Ser47 MKRRSSAsVSDSSGF Homo sapiens HeLa Cell
pmid sentence
Akt phosphorylated GST-RGC-32 in vitro, and CDC2 was also able to phosphorylate RGC-32.  Akt failed to phosphorylate RGC-32 S45A-S47A mutant. These data indicate that Ser 45 and Ser 47 may be the RGC-32 phosphorylation sites for Akt kinase.
Publications: 2 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation LARP6 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-273539 Ser451 QEKSPGTsPLLSRKM Homo sapiens HEK-293 Cell
pmid sentence
This strongly suggested that Akt dependent phosphorylation of LARP6 targets S451.S451A mutant of LARP6 acts as dominant negative protein in collagen synthesis. these results support the hypothesis that phosphorylation of S451 by Akt is a necessary step to activate productive synthesis type I collagen and suggests that secretion and modifications of type I collagen are impaired if LARP6 is not phosphorylated on S451.
Publications: 1 Organism: Homo Sapiens
+ AKT img/unknown.png phosphorylation ACLY 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-245259 Ser455 PAPSRTAsFSESRAD Rattus norvegicus Adipocyte
pmid sentence
Taken together, these results demonstrate that serine 454 of ATP-citrate lyase is a novel and major in vivo substrate for protein kinase B.
Publications: 1 Organism: Rattus Norvegicus
+ AKTdown-regulates img/direct_inhibition.png phosphorylation PDCD4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-143098 Ser457 RGRKRFVsEGDGGRL Homo sapiens
pmid sentence
Our results show that akt specifically phosphorylates ser(67) and ser(457) residues of pdcd4 in vitro and in vivo. We further show that phosphorylation of pdcd4 by akt causes nuclear translocation of pdcd4.
Identifier Residue Sequence Organism Cell Line
SIGNOR-150140 Ser67 KRRLRKNsSRDSGRG Homo sapiens
pmid sentence
Both akt and p70(s6k) phosphorylate pdcd4, allowing for binding of the e3-ubiquitin ligase beta-trcp and consequently ubiquitylation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-160982 Ser67 KRRLRKNsSRDSGRG Homo sapiens HEK-293 Cell
pmid sentence
Both akt and p70(s6k) phosphorylate pdcd4, allowing for binding of the e3-ubiquitin ligase beta-trcp and consequently ubiquitylation.
Publications: 3 Organism: Homo Sapiens
Tissue: Skin
+ AKTup-regulates img/direct-activation.png phosphorylation PFKFB3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-137241 Ser461 NPLMRRNsVTPLASP Homo sapiens
pmid sentence
We also found that AMP activated protein kinase and protein kinases A, B, and C catalyzed the phosphorylation of Ser-460 of HBP1, and that in addition both isoforms are phosphorylated at a second, as yet undetermined site by protein kinase C. However, none of the phosphorylations had any effect on the intrinsic kinetic characteristics of either enzymatic activity, and neither did point mutation (mimicking phosphorylation), deletion, and alternative-splice modification of the HBP1 carboxy-terminal region. Instead, these phosphorylations and mutations decreased the sensitivity of the 6PF2K to a potent allosteric inhibitor, phosphoenolpyruvate, which appears to be the major regulatory mechanism.
Publications: 1 Organism: Homo Sapiens
Pathways:Glycolysis and Gluconeogenesis
+ AKT img/unknown.png phosphorylation PFKFB2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-251484 Ser466 PVRMRRNsFTPLSSS Homo sapiens HeLa Cell
pmid sentence
14-3-3s bind directly to cardiac PFK-2 phosphorylated by PKB. PFK-2 was phosphorylated on both Ser466 and Ser483 by PKB. the precise mechanism of fru-2,6-P2 regulation by 14-3-3s is still puzzling.
Identifier Residue Sequence Organism Cell Line
SIGNOR-251485 Ser483 IRRPRNYsVGSRPLK Homo sapiens HeLa Cell
pmid sentence
14-3-3s bind directly to cardiac PFK-2 phosphorylated by PKB. PFK-2 was phosphorylated on both Ser466 and Ser483 by PKB. the precise mechanism of fru-2,6-P2 regulation by 14-3-3s is still puzzling.
Publications: 2 Organism: Homo Sapiens
Pathways:AMPK Signaling
+ AKTup-regulates activity img/direct-activation.png phosphorylation PFKFB2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-248031 Ser466 PVRMRRNsFTPLSSS Homo sapiens HeLa Cell
pmid sentence
These findings suggest that PKB-dependent binding of 14-3-3s to phospho-Ser483 of cardiac PFK-2 mediates the stimulation of glycolysis by growth factor.
Identifier Residue Sequence Organism Cell Line
SIGNOR-71419 Ser466 PVRMRRNsFTPLSSS Homo sapiens
pmid sentence
Heart 6-phosphofructo-2-kinase activation by insulin results from ser-466 and ser-483 phosphorylation and requires 3-phosphoinositide-dependent kinase-1, but not protein kinase b.
Identifier Residue Sequence Organism Cell Line
SIGNOR-103462 Ser483 IRRPRNYsVGSRPLK Homo sapiens HeLa Cell
pmid sentence
These findings suggest that pkb-dependent binding of 14-3-3s to phospho-ser483 of cardiac pfk-2 mediates the stimulation of glycolysis by growth factor.
Identifier Residue Sequence Organism Cell Line
SIGNOR-192260 Ser483 IRRPRNYsVGSRPLK Homo sapiens
pmid sentence
Akt-dependent activation of the heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (pfkfb2) isoenzyme by amino acids.
Publications: 4 Organism: Homo Sapiens
Tissue: Heart
Pathways:AMPK Signaling
+ AKTup-regulates activity img/direct-activation.png phosphorylation GABRB2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-262619 Ser472 SRLRRRAsQLKITIP Homo sapiens HEK-293 Cell
pmid sentence
Here we report that Akt phosphorylates, both in vitro and in vivo, the type A gamma-aminobutyric acid receptor (GABA(A)R), the principal receptor mediating fast inhibitory synaptic transmission in the mammalian brain. Akt-mediated phosphorylation increases the number of GABA(A)Rs on the plasma membrane surface, thereby increasing the receptor-mediated synaptic transmission in neurons. These results identify the GABA(A)R as a novel substrate of Akt, thereby linking Akt to the regulation of synaptic strength.
Publications: 1 Organism: Homo Sapiens
+ TBK1up-regulates img/direct-activation.png phosphorylation AKT 0.416
Identifier Residue Sequence Organism Cell Line
SIGNOR-172132 Ser473 RPHFPQFsYSASGTA Homo sapiens
pmid sentence
Upon mitogen stimulation, triggering of the innate immune response, re-exposure to glucose, or oncogene activation, tbk1 is recruited to the exocyst, where it activates akt. Akt is a direct tbk1 substrate that connects tbk1 to prosurvival signaling.
Publications: 1 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network, EBV infection, Inflammosome Activation, SARS-CoV INFLAMMATORY RESPONSE
+ mTORC2up-regulates activity img/direct-activation.png phosphorylation AKT 0.634
Identifier Residue Sequence Organism Cell Line
SIGNOR-217008 Ser473 RPHFPQFsYSASGTA Homo sapiens HT-29 Cell, A-549 Cell
pmid sentence
The rictor-mtor complex directly phosphorylated akt/pkb on ser473 in vitro and facilitated thr308 phosphorylation by pdk1
Identifier Residue Sequence Organism Cell Line
SIGNOR-252451 Ser477 PQFSYSAsGTA Homo sapiens MCF-7 Cell
pmid sentence
Phosphorylation of S477 and T479 at the Akt extreme carboxy terminus by cyclin-dependent kinase 2 (Cdk2)/cyclin A or mTORC2, under distinct physiological conditions, promotes Akt activation through facilitating, or functionally compensating for, S473 phosphorylation
Identifier Residue Sequence Organism Cell Line
SIGNOR-252448 Thr450 TAQMITItPPDQDDS Mus musculus MEF Cell
pmid sentence
MTORC2 phosphorylates newly synthesized Akt at the TM (Thr450) site to facilitate carboxyl-terminal folding and to stabilize Akt
Identifier Residue Sequence Organism Cell Line
SIGNOR-252454 Thr479 FSYSASGtA Homo sapiens MCF-7 Cell
pmid sentence
Phosphorylation of S477 and T479 at the Akt extreme carboxy terminus by cyclin-dependent kinase 2 (Cdk2)/cyclin A or mTORC2, under distinct physiological conditions, promotes Akt activation through facilitating, or functionally compensating for, S473 phosphorylation
Identifier Residue Sequence Organism Cell Line
SIGNOR-251982 Homo sapiens
pmid sentence
Mammalian TOR complex 1 (mTORC1) and mTORC2 exert their actions by regulating other important kinases, such as S6 kinase (S6K) and Akt.Recent findings have revealed novel important roles for mTORC2 in the phosphorylation of AGC kinase family members. mTORC2 phosphorylates and activates Akt, SGK, and PKC, which regulate cell survival, cell cycle progression and anabolism
Identifier Residue Sequence Organism Cell Line
SIGNOR-251983 Homo sapiens Blood Platelet
pmid sentence
Protein kinase B (PKB, Akt) is a Ser/Thr kinase involved in the regulation of cell survival, proliferation, and metabolism and is activated by dual phosphorylation on Thr(308) in the activation loop and Ser(473) in the hydrophobic motif. It plays a contributory role to platelet function, although little is known about its regulation. In this study, we investigated the role of the mammalian target of rapamycin complex (mTORC)-2 in Akt regulation using the recently identified small molecule ATP competitive mTOR inhibitors PP242 and Torin1.
Publications: 6 Organism: Homo Sapiens, Mus Musculus
Pathways:FLT3-ITD signaling, mTOR in cancer, MTOR Signaling, PI3K/AKT Signaling
+ PHLPP2down-regulates img/direct_inhibition.png dephosphorylation AKT 0.764
Identifier Residue Sequence Organism Cell Line
SIGNOR-135046 Ser473 RPHFPQFsYSASGTA Homo sapiens Glioblastoma Cell
pmid sentence
Here, we identify a protein phosphatase, ph domain leucine-rich repeat protein phosphatase (phlpp), that specifically dephosphorylates the hydrophobic motif of akt (ser473 in akt1), triggering apoptosis and suppressing tumor growth.[...] These data are consistent with phlpp terminating akt signaling by directly dephosphorylating and inactivating akt.
Publications: 1 Organism: Homo Sapiens
+ PPP2CAdown-regulates activity img/direct_inhibition.png dephosphorylation AKT 0.89
Identifier Residue Sequence Organism Cell Line
SIGNOR-248627 Ser473 RPHFPQFsYSASGTA Mus musculus
pmid sentence
Protein phosphatase 2A negatively regulates insulin's metabolic signaling pathway by inhibiting Akt (protein kinase B) activity in 3T3-L1 adipocytes
Identifier Residue Sequence Organism Cell Line
SIGNOR-248629 Thr308 KDGATMKtFCGTPEY Mus musculus NIH-3T3 Cell
pmid sentence
Protein phosphatase 2A negatively regulates insulin's metabolic signaling pathway by inhibiting Akt (protein kinase B) activity in 3T3-L1 adipocytes
Identifier Residue Sequence Organism Cell Line
SIGNOR-248655 Homo sapiens
pmid sentence
Overexpression of BTBD10 increased phosphorylation levels of Akts at both Thr(308) and Ser(473) while the reduction of the endogenous BTBD10 level resulted in a decrease in the phosphorylation levels of Akts. In vitro analysis indicated that BTBD10 bound to protein phosphatase 2A (PP2A) and inhibited dephosphorylation of Akts by PP2A.
Publications: 3 Organism: Mus Musculus, Homo Sapiens
Pathways:COVID-19 Causal Network, PI3K/AKT Signaling
+ LRRK2up-regulates img/direct-activation.png phosphorylation AKT 0.387
Identifier Residue Sequence Organism Cell Line
SIGNOR-205115 Ser473 RPHFPQFsYSASGTA Homo sapiens Neuron
pmid sentence
Expression of wild-type LRRK2 promoted neuronal survival against apoptosis through activation of the downstream effector, Akt by phosphorylation of Ser473. Phosphorylated Akt in turn inhibited FOXO 1 signaling
Identifier Residue Sequence Organism Cell Line
SIGNOR-244410 Homo sapiens Neuron
pmid sentence
Lrrk2 directly phosphorylates akt1 as a possible physiological substrate. These data establish that lrrk2 can protect neurons from apoptotic insult through a survival pathway in which lrrk2 signals to activate akt. Lrrk2-mediated phosphorylation of akt1 (ser473)
Publications: 2 Organism: Homo Sapiens
+ ILKup-regulates img/direct-activation.png phosphorylation AKT 0.771
Identifier Residue Sequence Organism Cell Line
SIGNOR-60115 Ser473 RPHFPQFsYSASGTA Homo sapiens
pmid sentence
Ilk can phosphorylate pkb-akt on serine-473, whereas kinase-deficient ilk severely inhibits endogenous phosphorylation of pkb-akt on serine-473, demonstrating that ilk is involved in agonist stimulated, pi(3)k-dependent, pkb-akt activation.
Publications: 1 Organism: Homo Sapiens
Pathways:Integrin Signaling
+ PPP1CCdown-regulates activity img/direct_inhibition.png dephosphorylation AKT 0.391
Identifier Residue Sequence Organism Cell Line
SIGNOR-248502 Ser473 RPHFPQFsYSASGTA Homo sapiens SK-BR-3 Cell
pmid sentence
Here, we identify PP1 as a serine/threonine phosphatase that associates with and dephosphorylates AKT in breast cancer cells|The heat shock protein 90 inhibitor geldanamycin and the ErbB inhibitor ZD1839 promote rapid PP1 phosphatase-dependent inactivation of AKT in ErbB2 overexpressing breast cancer cells
Publications: 1 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network
+ PTPAdown-regulates img/direct_inhibition.png dephosphorylation AKT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-170699 Ser473 RPHFPQFsYSASGTA Homo sapiens
pmid sentence
Consistent with previous reports (2830), we found that expression of sv40st, suppression of either pp2a c or b resulted in elevated levels of akt phosphorylation (ser473)
Publications: 1 Organism: Homo Sapiens
+ MTORup-regulates activity img/direct-activation.png phosphorylation AKT 0.928
Identifier Residue Sequence Organism Cell Line
SIGNOR-134185 Ser473 RPHFPQFsYSASGTA Homo sapiens HT-29 Cell, A-549 Cell
pmid sentence
The rictor-mtor complex directly phosphorylated akt/pkb on ser473 in vitro and facilitated thr308 phosphorylation by pdk1
Identifier Residue Sequence Organism Cell Line
SIGNOR-244417 Homo sapiens Blood Platelet
pmid sentence
Protein kinase B (PKB, Akt) is a Ser/Thr kinase involved in the regulation of cell survival, proliferation, and metabolism and is activated by dual phosphorylation on Thr(308) in the activation loop and Ser(473) in the hydrophobic motif. It plays a contributory role to platelet function, although little is known about its regulation. In this study, we investigated the role of the mammalian target of rapamycin complex (mTORC)-2 in Akt regulation using the recently identified small molecule ATP competitive mTOR inhibitors PP242 and Torin1.
Publications: 2 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, KIT in AML, miRNA in AML, FLT3-ITD in AML, FLT3-ITD signaling, Glioblastoma Multiforme, Leptin Signaling, MTOR Signaling, Pancreatic ductal adenocarcinoma (PDA)
+ PPP1CBdown-regulates activity img/direct_inhibition.png dephosphorylation AKT 0.394
Identifier Residue Sequence Organism Cell Line
SIGNOR-248575 Ser473 RPHFPQFsYSASGTA Homo sapiens
pmid sentence
Here, we identify PP1 as a serine/threonine phosphatase that associates with and dephosphorylates AKT in breast cancer cells|The heat shock protein 90 inhibitor geldanamycin and the ErbB inhibitor ZD1839 promote rapid PP1 phosphatase-dependent inactivation of AKT in ErbB2 overexpressing breast cancer cells
Publications: 1 Organism: Homo Sapiens
+ ILKup-regulates activity img/direct-activation.png phosphorylation AKT 0.771
Identifier Residue Sequence Organism Cell Line
SIGNOR-250261 Ser473 RPHFPQFsYSASGTA in vitro
pmid sentence
ILK Phosphorylates PKB/Akt on Serine 473 To become fully activated, PKB/Akt requires phosphorylation at two sites, threonine 308 and serine 473, in a phosphatidylinositol (PI) 3-kinase-dependent manner.
Publications: 1 Organism: In Vitro
Pathways:Integrin Signaling
+ PHLPP1down-regulates img/direct_inhibition.png dephosphorylation AKT 0.751
Identifier Residue Sequence Organism Cell Line
SIGNOR-135005 Ser473 RPHFPQFsYSASGTA Homo sapiens Glioblastoma Cell
pmid sentence
Here, we identify a protein_ phosphatase, ph domain leucine-rich repeat protein_ phosphatase_ (phlpp), that specifically_ dephosphorylates_ the hydrophobic motif of_ akt_ (ser473 in akt1), triggering_ apoptosis_ and suppressing_ tumor_ growth.
Publications: 1 Organism: Homo Sapiens
+ PPP1CAdown-regulates activity img/direct_inhibition.png dephosphorylation AKT 0.435
Identifier Residue Sequence Organism Cell Line
SIGNOR-248559 Ser473 RPHFPQFsYSASGTA Homo sapiens SK-BR-3 Cell
pmid sentence
Here, we identify PP1 as a serine/threonine phosphatase that associates with and dephosphorylates AKT in breast cancer cells|The heat shock protein 90 inhibitor geldanamycin and the ErbB inhibitor ZD1839 promote rapid PP1 phosphatase-dependent inactivation of AKT in ErbB2 overexpressing breast cancer cells
Publications: 1 Organism: Homo Sapiens
+ PRKDCup-regulates activity img/direct-activation.png phosphorylation AKT 0.744
Identifier Residue Sequence Organism Cell Line
SIGNOR-252447 Ser473 RPHFPQFsYSASGTA Homo sapiens HUVEC Cell
pmid sentence
DNA-PK phosphorylates HM Ser473 of PKB. However, we also noted similar patterns in T loop Thr308 phosphorylation after _-IR []his function is apparently restricted to the PKBalpha isoform
Publications: 1 Organism: Homo Sapiens
Pathways:Cell cycle: G2/M phase transition
+ CCN4up-regulates activity img/direct-activation.png phosphorylation AKT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-256269 Ser473 RPHFPQFsYSASGTA Rattus norvegicus
pmid sentence
Here it is shown that WISP-1 can activate the antiapoptotic Akt/PKB signaling pathway.
Publications: 1 Organism: Rattus Norvegicus
+ CyclinA2/CDK2up-regulates activity img/direct-activation.png phosphorylation AKT 0.425
Identifier Residue Sequence Organism Cell Line
SIGNOR-252450 Ser477 PQFSYSAsGTA Homo sapiens MCF-7 Cell
pmid sentence
Phosphorylation of S477 and T479 at the Akt extreme carboxy terminus by cyclin-dependent kinase 2 (Cdk2)/cyclin A or mTORC2, under distinct physiological conditions, promotes Akt activation through facilitating, or functionally compensating for, S473 phosphorylation
Identifier Residue Sequence Organism Cell Line
SIGNOR-252453 Thr479 FSYSASGtA Homo sapiens MCF-7 Cell
pmid sentence
Phosphorylation of S477 and T479 at the Akt extreme carboxy terminus by cyclin-dependent kinase 2 (Cdk2)/cyclin A or mTORC2, under distinct physiological conditions, promotes Akt activation through facilitating, or functionally compensating for, S473 phosphorylation
Publications: 2 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation HJURP 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-262623 Ser486 GLETRRLsLPSSKAK in vitro
pmid sentence
These data define a new protein complex in mammalian cells where 14‐3‐3 associates with FAKTS through phosphorylated S479. Our research identifies a widely expressed eukaryotic protein FAKTS, as a new Akt/PKB substrate localized in the nucleus. Akt/PKB promotes FAKTS association with 14‐3‐3, placing FAKTS under the control of 14‐3‐3 proteins. FAKTS may play an important role in transmitting Akt/PKB‐mediated signals in the complex network of intracellular signal transduction.
Publications: 1 Organism: In Vitro
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation PTPN1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-235411 Ser50 RNRYRDVsPFDHSRI Mus musculus NIH-3T3 Cell
pmid sentence
Phosphorylation of ptp1b at ser(50) by akt impairs its ability to dephosphorylate the insulin receptor.
Publications: 1 Organism: Mus Musculus
Pathways:Insulin Signaling
+ AKT img/unknown.png phosphorylation CARHSP1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-137430 Ser52 TRRTRTFsATVRASQ Homo sapiens
pmid sentence
These and other results demonstrate that crhsp24 is phosphorylated at ser52 by pkbalpha in response to igf-1, at ser52 by pkbalpha and rsk in response to egf
Publications: 1 Organism: Homo Sapiens
Tissue: Kidney
+ AKTup-regulates img/direct-activation.png phosphorylation CTNNB1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244222 Ser552 QDTQRRTsMGGTQQQ Homo sapiens
pmid sentence
Phosphorylation of beta-catenin by akt promotes beta-catenin transcriptional activity|we have demonstrated that akt phosphorylates beta-catenin at ser552 in vitro and in vivo.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, FLT3 in AML, FLT3-ITD in AML, FLT3-ITD signaling, Hepatocellular Tumor, Pancreatic ductal adenocarcinoma (PDA), Rhabdomyosarcoma, Thyroid cancer, WNT/FLT3
+ AKT img/unknown.png phosphorylation ACAP1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-141343 Ser554 SIRPRPGsLRSKPEP Homo sapiens
pmid sentence
Akt phosphorylates s554 in acap1
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates quantity img/direct-activation.png phosphorylation RANBP3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-276150 Ser57 HGTGHPEsAGEHALE Homo sapiens HEK-293 Cell
pmid sentence
Akt phosphorylates RanBP3 at Serine 58 residue in vitro and in vivo. RanBP3 phosphorylation increases its affinity towards Ran
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation PPARGC1A 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-155532 Ser571 RMRSRSRsFSRHRSC Homo sapiens
pmid sentence
Here we describe a mechanism by which insulin, through the intermediary protein kinase akt2/protein kinase b (pkb)-beta, elicits the phosphorylation and inhibition of the transcriptional coactivator peroxisome proliferator-activated receptor-coactivator 1alpha (pgc-1alpha), a global regulator of hepatic metabolism during fasting / phosphorylation of pgc-1alpha At ser570 Is required for akt to inhibit recruitment of pgc-1alpha To chromatin.
Publications: 1 Organism: Homo Sapiens
Tissue: Liver
Pathways:AMPK Signaling, MTOR Signaling
+ AKTup-regulates activity img/direct-activation.png phosphorylation CCNF 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-266361 Ser577 TKRKRENsLQEDRGS Homo sapiens U2-OS Cell
pmid sentence
AKT phosphorylation of cyclin F enhances its stability and promotes assembly into productive E3 ligase complexes.
Identifier Residue Sequence Organism Cell Line
SIGNOR-266360 Thr31 RRRPRNLtILSLPED Homo sapiens U2-OS Cell
pmid sentence
AKT phosphorylation of cyclin F enhances its stability and promotes assembly into productive E3 ligase complexes.
Publications: 2 Organism: Homo Sapiens
+ AKT img/unknown.png phosphorylation YWHAZ 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244381 Ser58 VVGARRSsWRVVSSI Homo sapiens HEK-293 Cell
pmid sentence
Ese data indicate that pkb/akt phosphorylates ser-58 on 14-3-3zeta both in vitro and in intact cells. The functional relevance of this phosphorylation remains to be determined.
Publications: 1 Organism: Homo Sapiens
+ AKT img/unknown.png phosphorylation TBC1D4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-245268 Ser588 RMRGRLGsVDSFERS Mus musculus 3T3-L1 Cell
pmid sentence
To determine directly whether AS160 was a substrate for Akt, we examined the phosphorylation of recombinant AS160, as well as mutant forms with Ser-588, Thr-642, or both converted to Ala, by recombinant Akt 1.
Identifier Residue Sequence Organism Cell Line
SIGNOR-148342 Thr642 QFRRRAHtFSHPPSS Homo sapiens
pmid sentence
14-3-3 proteins interact with as160 in an insulin- and akt-dependent manner via an akt phosphorylation site, thr-642.
Publications: 2 Organism: Mus Musculus, Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation PRPH 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-262627 Ser59 SSSVRLGsFRSPRAG Homo sapiens HEK-293 Cell
pmid sentence
Here we demonstrate that peripherin, which is a peripheral nervous system neuron-specific intermediate filament protein, is a novel Akt substrate, and that Ser66 of peripherin is the phosphorylation site. Peripherin phosphorylation is apparently induced in motor neurons after nerve injury, suggesting that the Akt-mediated peripherin phosphorylation may play a role in motor nerve regeneration.
Publications: 1 Organism: Homo Sapiens
+ AKT img/unknown.png phosphorylation SH2B2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-251487 Ser598 SARSRSNsAERLLEA Mus musculus
pmid sentence
Serine 588 of APS is a newly identified target for protein kinase B in intact cells and in vitro. The precise function of this PKB-mediated phosphorylation event is not entirely clear but may be responsible for regulating cellular localization and will be the subject of future investigation.
Publications: 1 Organism: Mus Musculus
+ AKTup-regulates activity img/direct-activation.png phosphorylation SH2B2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-248042 Ser598 SARSRSNsAERLLEA Mus musculus
pmid sentence
This study identifies APS as a novel physiological substrate for PKB and the first serine phosphorylation site on APS
Publications: 1 Organism: Mus Musculus
+ AKTup-regulates img/direct-activation.png phosphorylation COPS6 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-199254 Ser60 DHWIRMRsQEGRPVQ Homo sapiens
pmid sentence
Mechanistic studies show that akt causes csn6 phosphorylation at ser 60, which, in turn, reduces ubiquitin-mediated protein degradation of csn6.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation NUAK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-95247 Ser600 PARQRIRsCVSAENF Homo sapiens
pmid sentence
Ser(600) in ark5 was found to be phosphorylated by active akt resulting in the activation of kinase activity.
Publications: 1 Organism: Homo Sapiens
+ AKT img/unknown.png phosphorylation NIBAN1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-197053 Ser602 ASPARRAsAILPGVL Homo sapiens
pmid sentence
We demonstrate here that ultraviolet irradiation induces phosphorylation of niban at s602 by akt, which increases the association of niban with nucleophosmin and disassociation of nucleophosmin from the mdm2 complex.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation NOS3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-251628 Ser615 SYKIRFNsISCSDPL Homo sapiens Vascular Endothelium
pmid sentence
The phosphorylation of both S617 and S635 have also been shown to promote increased eNOS-derived NO release (Michell et al., 2002). The phosphorylaiton of S617 can be induced by PKA or Akt activity, and may serve to sensitize eNOS to calmodulin binding and modulate the phosphorylation of other eNOS sites
Publications: 1 Organism: Homo Sapiens
Pathways:VEGF Signaling
+ AKTup-regulates activity img/direct-activation.png phosphorylation ILF3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-168169 Ser647 RGRGRGGsIRGRGRG Homo sapiens
pmid sentence
Upon T cell activation, NF90 translocates from the nucleus into the cytoplasm, where it binds to the AU-rich element-containing 3' untranslated regions of IL-2 mRNA and stabilizes it.|Our previous work showed that CD28 costimulation of T cells activated AKT to phosphorylate NF90 at Ser647 and caused NF90 to undergo nuclear export and stabilize IL-2 mRNA.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation MAP3K11 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-96062 Ser674 PGRERGEsPTTPPTP Homo sapiens Neuron
pmid sentence
Negative regulation of mixed lineage kinase 3 by protein kinase b/akt leads to cell survivalthe expression of activated akt1 inhibits mlk3-mediated cell death in a manner dependent on serine 674 phosphorylation.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation BRCA1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244164 Ser694 QTSKRHDsDTFPELK Homo sapiens Breast Cancer Cell
pmid sentence
We identify a novel akt phosphorylation site in brca1 at s694 which is responsive to activation of these signaling pathways. These data suggest akt phosphorylation of brca1 increases total protein expression by preventing proteasomal degradation
Identifier Residue Sequence Organism Cell Line
SIGNOR-244168 Thr509 LKRKRRPtSGLHPED Homo sapiens Breast Cancer Cell
pmid sentence
Phosphatidylinositol 3-kinase/akt signaling enhances nuclear localization and transcriptional activity of brca1. mutation of threonine 509 in brca1, the site of akt phosphorylation, to an alanine, attenuates the ability of heregulin to induce brca1 nuclear accumulation
Publications: 2 Organism: Homo Sapiens
Pathways:Cell cycle: G2/M phase transition
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation RAC1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-248036 Ser71 YDRLRPLsYPQTDVF Homo sapiens SK-MEL-2 Cell
pmid sentence
The results suggest that Akt kinase of the phosphoinositide 3-kinase signal transduction pathway phosphorylates serine 71 of Rac1 as one of its authentic substrates and modulates the Rac1 signal transduction pathway through phosphorylation.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, FLT3-ITD in AML, WNT/FLT3
+ AKTup-regulates activity img/direct-activation.png phosphorylation TBX3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244353 Ser719 AEKEAATsELQSIQR Homo sapiens
pmid sentence
We have identified TBX3 as a key substrate of AKT3 in melanomagenesis. we have identified the AKT3 target site at serine residue 720 in the TBX3 protein and show that this site is phosphorylated in vivo. the phosphorylation at S720 promotes TBX3 protein stability, nuclear localization, transcriptional repression of E-cadherin, and its role in cell migration and invasion.
Publications: 1 Organism: Homo Sapiens
+ AKT img/unknown.png phosphorylation DLC1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-247997 Ser766 VTRTRSLsACNKRVG Rattus norvegicus Adipocyte
pmid sentence
We have demonstrated that Ser-322 is phosphorylated upon insulin stimulation of intact cells and that this site is directly phosphorylated in vitro by PKB and ribosomal S6 kinase, members of the AGC (protein kinases A, G, and C) family of insulin-stimulated protein kinases
Publications: 1 Organism: Rattus Norvegicus
+ AKTup-regulates quantity by stabilization img/direct-activation.png phosphorylation ATXN1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-251468 Ser775 ATRKRRWsAPESRKL Homo sapiens HeLa Cell
pmid sentence
Interaction of Ataxin-1 and 14-3-3 Requires Akt Phosphorylation at S776. 14-3-3 protein, a multifunctional regulatory molecule, mediates the neurotoxicity of ataxin-1 by binding to and stabilizing ataxin-1, thereby slowing its normal degradation.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation AR 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244136 Ser792 CVRMRHLsQEFGWLQ Chlorocebus aethiops COS-1 Cell
pmid sentence
Akt suppresses androgen-induced apoptosis by phosphorylating and inhibiting androgen receptor. Here, we demonstrate that akt phosphorylates the androgen receptor (ar) at ser-210 and ser-790
Publications: 1 Organism: Chlorocebus Aethiops
Pathways:Prostate Cancer
+ AKTdown-regulates img/direct_inhibition.png phosphorylation MAP2K4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244285 Ser80 IERLRTHsIESSGKL Homo sapiens HEK-293 Cell
pmid sentence
Akt phosphorylated sek1 on serine 78.
Publications: 1 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network, EGFR Signaling
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation MAP2K4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-275980 Ser80 IERLRTHsIESSGKL Homo sapiens HEK-293T Cell
pmid sentence
Akt (Protein Kinase B) Negatively Regulates SEK1 by Means of Protein Phosphorylation. In vitro and in vivo (32)P labeling indicated that Akt phosphorylated SEK1 on serine 78. The SEK1 mutant SEK1(S78A) was resistant to Akt-induced inhibition. 
Publications: 1 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network, EGFR Signaling
+ AKTdown-regulates img/direct_inhibition.png phosphorylation HSPB1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-186772 Ser82 RALSRQLsSGVSEIR Homo sapiens
pmid sentence
First, the akt1, akt2, and akt3 isoforms can bind directly to hsp27 and can be found in a complex with p38 mapk, mk2, and hsp27 [98_100]. Second, rane and colleagues showed that akt could phosphorylate hsp27 at ser-82, but not ser-15 or ser-78, in vitro, while co-expression of an active akt mutant and hsp27 in hek cells resulted in hsp27 phosphorylation at the same residue.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation MAP3K5 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-104646 Ser83 ATRGRGSsVGGGSRR Homo sapiens HEK-293 Cell
pmid sentence
Akt phosphorylates and negatively regulates apoptosis signal-regulating kinase 1 akt decreased ask1 kinase activity stimulated by both oxidative stress and overexpression in 293 cells by phosphorylating a consensus akt site at serine 83 of ask1.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates quantity by stabilization img/direct-activation.png phosphorylation XIAP 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244377 Ser87 VGRHRKVsPNCRFIN Homo sapiens
pmid sentence
Akt, including akt1 and akt2, interacts with and phosphorylates x-linked inhibitor of apoptosis protein (xiap) at residue serine-87 in vitro and in vivo. Phosphorylation of xiap by akt protects xiap from ubiquitination and degradation in response to cisplatin.
Publications: 1 Organism: Homo Sapiens
Tissue: Ovary Cancer Cell
Pathways:Inhibition of Apoptosis
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation BCL2L11 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-141581 Ser87 FIFMRRSsLLSRSSS Homo sapiens
pmid sentence
Recombinant Akt could directly phosphorylate a GST-Bim(EL) fusion protein and identified the Akt phosphorylation site in the Bim(EL) domain as Ser(87). Further, we demonstrated that cytokine stimulation promotes Bim(EL) binding to 14-3-3 proteins. Finally, we show that mutation of Ser(87) dramatically increases the apoptotic potency of Bim(EL).
Publications: 1 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network, Inhibition of Apoptosis, SARS-COV APOPTOSIS
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation GSK3B 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-245428 Ser9 SGRPRTTsFAESCKP Homo sapiens
pmid sentence
Active AKT, a common mediator of cell survival signals induced by radiation through multiple intracellular signaling pathways,11, 12 suppresses apoptosis. AKT positively regulates cyclin D1 expression through inactivation of glycogen synthase kinase 3_ (GSK3_). The AKT-mediated phosphorylation of glycogen synthase kinase 3_ on serine9 decreases its kinase activity for Thr286 of cyclin D1, which inhibits the nuclear export and the cytoplasmic proteasomal degradation of cyclin D1
Identifier Residue Sequence Organism Cell Line
SIGNOR-242578 Ser9 SGRPRTTsFAESCKP Homo sapiens HEK-293 Cell
pmid sentence
Evidence that the inhibition of glycogen synthase kinase-3_ by IGF-1 is mediated by PDK1/PKB-induced phosphorylation of Ser-9
Publications: 2 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, AML_TRIPLETS, FLT3-ITD signaling, Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, Insulin Signaling, MTOR Signaling, Triple mutant AML, PI3K/AKT Signaling, WNT/FLT3
+ AKTdown-regulates img/direct_inhibition.png phosphorylation ZFP36L1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244385 Ser92 RFRDRSFsEGGERLL Homo sapiens
pmid sentence
Here we report that protein kinase b (pkb/akt) stabilizes are transcripts by phosphorylating brf1 at serine 92 (s92). Recombinant brf1 promoted in vitro decay of are-containing mrna (are-mrna), yet phosphorylation by pkb impaired this activity.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation TSC2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244369 Ser939 SFRARSTsLNERPKS Mus musculus NIH-3T3 Cell
pmid sentence
We demonstrate that, upon activation of PI3K, tuberin is phosphorylated on consensus recognition sites for PI3K-dependent S/T kinases. Moreover, Akt/PKB can phosphorylate tuberin in vitro and in vivo. We also show that S939 and T1462 of tuberin are PI3K-regulated phosphorylation sites and that T1462 is constitutively phosphorylated in PTEN(-/-) tumor-derived cell lines.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244365 Thr1462 GLRPRGYtISDSAPS Mus musculus NIH-3T3 Cell
pmid sentence
We demonstrate that, upon activation of PI3K, tuberin is phosphorylated on consensus recognition sites for PI3K-dependent S/T kinases. Moreover, Akt/PKB can phosphorylate tuberin in vitro and in vivo. We also show that S939 and T1462 of tuberin are PI3K-regulated phosphorylation sites and that T1462 is constitutively phosphorylated in PTEN(-/-) tumor-derived cell lines.
Publications: 2 Organism: Mus Musculus
+ AKTdown-regulates img/direct_inhibition.png phosphorylation RARA 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-143721 Ser96 FVCQDKSsGYHYGVS Homo sapiens Lung Cancer Cell
pmid sentence
We report that akt, which is constitutively activated in nsclc cells, phosphorylates raralpha and inhibits its transactivation. / mutation of ser96 to alanine abrogated the suppressive effect of akt.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, ASXL1 in AML
+ AKTup-regulates img/direct-activation.png phosphorylation AGAP2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244132 Ser985 THLSRVRsLDLDDWP Homo sapiens Prostate Gland Cancer Cell
pmid sentence
In addition, we have found that activated akt can bind and phosphorylate ggap2 at serine 629, which enhances gtp binding by ggap2.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation IRAK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-248008 Thr100 LRARDIItAWHPPAP Homo sapiens HEK-293 Cell
pmid sentence
CaMKKc and Akt overexpression increases IRAK1 phosphorylation at Thr100, and point mutation of this site abrogates the inhibitory effect of Akt on IRAK1-mediated NF-kappaB activation.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation ADAR 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-266357 Thr1033 RLGERLRtMSCSDKI in vitro
pmid sentence
AKT-dependent phosphorylation of the adenosine deaminases ADAR-1 and -2 inhibits deaminase activity. Using site-directed mutagenesis of suspected AKT phosphorylation sites, AKT was found to primarily phosphorylate ADAR1p110 and ADAR2 on T738 and T553, respectively, and overexpression of the phosphomimic mutants ADAR1p110 (T738D) and ADAR2 (T553D) resulted in a 50-100% reduction in editase activity.
Publications: 1 Organism: In Vitro
+ AKTdown-regulates img/direct_inhibition.png phosphorylation STK3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244345 Thr117 IIRLRNKtLIEDEIA Homo sapiens
pmid sentence
We determined that mst2 phosphorylation by akt limits mst2 activity in two ways: first, by blocking its binding to rassf1a and by promoting its association into the raf-1 inhibitory complex, and second, by preventing homodimerization of mst2, which is needed for its activation. we identified t117 and t384 as akt phosphorylation sites in mst2.
Identifier Residue Sequence Organism Cell Line
SIGNOR-164298 Thr117 IIRLRNKtLIEDEIA Homo sapiens Breast Cancer Cell
pmid sentence
Akt phosphorylates mst2 at thr117 in vitro and in vivo, which leads to mst2 cleavage and kinase activity as well as nuclear translocation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244349 Thr384 GTMKRNAtSPQVQRP Homo sapiens
pmid sentence
We determined that mst2 phosphorylation by akt limits mst2 activity in two ways: first, by blocking its binding to rassf1a and by promoting its association into the raf-1 inhibitory complex, and second, by preventing homodimerization of mst2, which is needed for its activation. we identified t117 and t384 as akt phosphorylation sites in mst2.
Publications: 3 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation STK4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-161829 Thr120 IIRLRNKtLTEDEIA Homo sapiens
pmid sentence
Akt interacts with mst1 and phosphorylates a highly conserved residue threonine 120 of mst1, which leads to inhibition of its kinase activity and nuclear translocation as well as the autophosphorylation of thr(183).
Identifier Residue Sequence Organism Cell Line
SIGNOR-201121 Thr387 TMKRRDEtMQPAKPS Homo sapiens
pmid sentence
Full activation of mst1 requires an activation cleavage that is prevented by the phosphorylation of thr-387 by akt.
Publications: 2 Organism: Homo Sapiens
+ AKTdown-regulates quantity img/direct_inhibition.png phosphorylation RNF11 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-248085 Thr135 DWLMRSFtCPSCMEP Homo sapiens WM-239 Cell
pmid sentence
Upon inhibition of the AKT pathway or mutation of T135, the phosphorylation at one of these sites is virtually eliminated, suggesting that AKT may phosphorylate RNF11 at T135. Moreover, RNF11 is phosphorylated by AKT in vitro and is recognized by phospho-AKT substrate antibodies. RNF11 shows enhanced binding to 14-3-3 in WM239 cells compared with that seen in the parental WM35 cells which have low AKT activity
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates quantity by stabilization img/direct-activation.png phosphorylation CDKN1A 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244184 Thr145 QGRKRRQtSMTDFYH Homo sapiens
pmid sentence
Whereas akt1 phosphorylates p21, inducing its release from cdk2 and cytoplasmic localization as previously described for akt.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244180 Thr145 QGRKRRQtSMTDFYH Homo sapiens
pmid sentence
Whereas akt1 phosphorylates p21, inducing its release from cdk2 and cytoplasmic localization as previously described for akt, akt2 binds p21 in the region spanning the t145 site of p21, thus competing with phosphorylation by akt1 and inducing its accumulation in the nucleus. These distinct roles of akt/pkb isoforms in modulating proliferation and p21 have important implications for the development of drugs aimed at inhibiting cancer cell proliferation.[...] We next investigated if phosphorylation of p21-t145 interfered with akt2 binding. As shown in fig. ?Fig.8e8e (right lane), phosphorylation of p21 on t145 effectively prevented akt2 interaction.
Publications: 2 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, FLT3-ITD in AML, FLT3-ITD signaling, Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, Rhabdomyosarcoma
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation FOXA2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-254974 Thr156 KTYRRSYtHAKPPYS Homo sapiens
pmid sentence
Foxa-2 physically interacts with Akt, a key mediator of the phosphatidylinositol 3-kinase pathway and is phosphorylated at a single conserved site (T156) that is absent in Foxa-1 and Foxa-3 proteins. This Akt phosphorylation site in Foxa-2 is highly conserved from mammals to insects. Mutant Foxa-2T156A is resistant to Akt-mediated phosphorylation, nuclear exclusion, and transcriptional inactivation of Foxa-2-regulated gene expression.
Publications: 1 Organism: Homo Sapiens
Pathways:FAP: Insulin-mediated adipogenesis
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation CDKN1B 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244202 Thr157 GIRKRPAtDDSSTQN Homo sapiens
pmid sentence
Mtor may promote g1 progression in part through sgk1 activation and deregulate the cell cycle in cancers through both akt- and sgk-mediated p27 t157 phosphorylation and cytoplasmic p27 mislocalization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244194 Thr198 PGLRRRQt Homo sapiens
pmid sentence
Because Thr198-phosphorylated p27Kip1 was localized only in the cytoplasm, Akt might promote 14-3-3 binding to p27Kip1 by phosphorylation at Thr198, allowing its cytoplasmic localization and degradation.
Publications: 2 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, Integrin Signaling
+ AKTdown-regulates img/direct_inhibition.png phosphorylation CDCA7 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-199776 Thr163 SRRPRRRtFPGVASR Homo sapiens
pmid sentence
The prosurvival kinase akt phosphorylates cdca7 at threonine 163, promoting binding to 14-3-3, dissociation from myc, and sequestration to the cytoplasm. we have mapped the domains of interaction and have discovered that akt phosphorylates cdca7 near this contact region, leading to loss of its association with myc, binding to 14-3-3 proteins, and exclusion from the nucleus.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation PLN 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-252035 Thr17 SAIRRAStIEMPQQA Mus musculus Cardiac Muscle Cell Line
pmid sentence
Akt interacts with and phosphorylates PLN at Thr(17), the Ca(2+)-calmodulin-dependent kinase IIdelta site, whereas silencing Akt signaling, through the knock-out of phosphatidylinositol-dependent kinase-1, resulted in reduced phosphorylation of PLN at Thr(17).
Publications: 1 Organism: Mus Musculus
+ AKTup-regulates img/direct-activation.png phosphorylation ALYREF 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-179054 Thr219 GGGTRRGtRGGARGR Homo sapiens
pmid sentence
Nuclear akt directly binds aly and phosphorylates it on the t219 residue. gfp-aly t219d displayed comparable activity to gfp control and wild-type aly, indicating that aly phosphorylation by akt is sufficient to enhance mrna export.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation CHUK 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244210 Thr23 EMRERLGtGGFGNVC Homo sapiens
pmid sentence
Although there are likely to be multiple levels of crosstalk between the pi3k-akt and nf-kb pathways, one mechanism has been attributed to direct phosphorylation of the amino acid residue t23 on ikb kinase alfa (ikkalfa) by akt, thereby leading to activation of this kinase upstream of nf-kb akt mediates ikkalpha phosphorylation at threonine 23 akt transiently associates in vivo with ikk and induces ikk activation. Akt mediates ikkalfa phosphorylation at threonine 23.Akt phosphorylates ikkalpha on t23, and this phosphorylation event is a prerequisite for the phosphorylation of p65 at s534 by ikkalpha and beta
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates quantity by stabilization img/direct-activation.png phosphorylation POU5F1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-242092 Thr235 QARKRKRtSIENRVR Homo sapiens NCCIT Cell
pmid sentence
Here we show that in ECCs, Akt phosphorylated the master pluripotency factor Oct4 at threonine 235, and that the levels of phosphorylated Oct4 in ECCs correlated with resistance to apoptosis and tumorigenic potential. Phosphorylation of Oct4 increased its stability and facilitated its nuclear localization and its interaction with Sox2, which promoted the transcription of the core stemness genes POU5F1 and NANOG.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation S1PR1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-110845 Thr236 RTRSRRLtFRKNISK Homo sapiens
pmid sentence
Activated akt binds to edg-1 and phosphorylates the third intracellular loop at the t(236) residue. Transactivation of edg-1 by akt is not required for g(i)-dependent signaling but is indispensable for rac activation, cortical actin assembly, and chemotaxis
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation AKT1S1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-236929 Thr246 LPRPRLNtSDFQKLK Homo sapiens HEK-293 Cell
pmid sentence
Treatment of these cells with 4-hydroxytamoxifen stimulated the phosphorylation of wt PRAS40 but not the mutant PRAS40 in which Thr-246 was mutated. These results demonstrate that activation of Akt alone is sufficient to induce phosphorylation of PRAS40
Publications: 1 Organism: Homo Sapiens
Pathways:MTOR Signaling
+ PDPK1up-regulates activity img/direct-activation.png phosphorylation AKT 0.738
Identifier Residue Sequence Organism Cell Line
SIGNOR-134477 Thr308 KDGATMKtFCGTPEY Mus musculus
pmid sentence
Akt1 and akt2 are phosphorylated and activated by the protein kinase pdk1 at thr-308 or thr-309, respectively, in the activation t-loop, and further activation occurs through phosphorylation at ser-473 or ser-474, respectively. In this paper, we demonstrate that this is indeed the case, and report the purification and initial characterization of a 3 phosphoinositide-dependent protein kinase, pdk1, which activates pkb by phosphorylating it at thr308. Akt is directly phosphorylated and activated by pdk1. Akt/pkb activation requires the phosphorylation of thr308 in the activation loop by the phosphoinositide-dependent kinase 1 (pdk1).
Identifier Residue Sequence Organism Cell Line
SIGNOR-244421 Thr308 KDGATMKtFCGTPEY Rattus norvegicus
pmid sentence
Protein kinase B (PKB) is activated by phosphorylation of Thr308 and of Ser473. Thr308 is phosphorylated by the 3-phosphoinositide-dependent protein kinase-1 (PDK1) but the identity of the kinase that phosphorylates Ser473 (provisionally termed PDK2) is unknown.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244480 Thr308 KDGATMKtFCGTPEY Homo sapiens
pmid sentence
The activation of PKBbeta and PKBamma by PDK11 was accompanied by the phosphorylation of the residues equivalent to Thr308 in PKBalpha, namely Thr309 (PKBbeta) and Thr305 (PKBgamma)
Identifier Residue Sequence Organism Cell Line
SIGNOR-243203 Thr308 KDGATMKtFCGTPEY Homo sapiens
pmid sentence
Akt/PKB activation requires the phosphorylation of Thr308 in the activation loop by the phosphoinositide-dependent kinase 1 (PDK1) and Ser473 within the carboxyl-terminal hydrophobic motif by an unknown kinase.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244469 Homo sapiens
pmid sentence
3-phosphoinositide-dependent kinase 1 (PDK1) phosphorylates the activation loop of a number of protein serine/threonine kinases of the AGC kinase superfamily, including protein kinase B (PKB; also called Akt),
Publications: 5 Organism: Mus Musculus, Rattus Norvegicus, Homo Sapiens
Tissue: Brain, Muscle, Skeletal Muscle, Myotube
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, KIT in AML, miRNA in AML, AMPK Signaling, B-cell activation, EGFR Signaling, ErbB receptors in cancer, FAP: Insulin-mediated adipogenesis, FLT3-ITD signaling, Glioblastoma Multiforme, Hepatocellular Tumor, Insulin Signaling, Luminal Breast Cancer, mTOR in cancer, Malignant Melanoma, MTOR Signaling, Non-small-cell lung cancer (NSCLC), Prostate Cancer, PI3K/AKT Signaling, Rhabdomyosarcoma, RTKs in cancer, Thyroid cancer, T cell activation, VEGF Signaling
+ CAMKK1up-regulates activity img/direct-activation.png phosphorylation AKT 0.388
Identifier Residue Sequence Organism Cell Line
SIGNOR-250714 Thr308 KDGATMKtFCGTPEY
pmid sentence
Protein kinase B (PKB) was recently reported to be activated on the phosphorylation of Thr(308) by Ca(2+)/calmodulin-dependent protein kinase kinase alpha (CaM-kinase kinase alpha), suggesting that PKB was regulated through not only the phosphoinositide 3-kinase pathway but also the Ca(2+)/calmodulin protein kinase pathway.
Publications: 1
+ PPP2R5Bdown-regulates img/direct_inhibition.png dephosphorylation AKT 0.647
Identifier Residue Sequence Organism Cell Line
SIGNOR-144808 Thr308 KDGATMKtFCGTPEY Homo sapiens
pmid sentence
Activation of pp2a is the intermediate step between the abeta-ceramide cascade and the subsequent inactivation of akt.
Publications: 1 Organism: Homo Sapiens
+ GSK3Adown-regulates activity img/direct_inhibition.png phosphorylation AKT 0.623
Identifier Residue Sequence Organism Cell Line
SIGNOR-252455 Thr312 TMKTFCGtPEYLAPE Mus musculus Th17 Cell
pmid sentence
GSK3_ negatively regulates AKT activation by phosphorylating AKT at T312 in the substrate binding site, which inhibited IL-1-induced AKT activation and function.
Publications: 1 Organism: Mus Musculus
+ AKT img/unknown.png phosphorylation LTB4R2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-177019 Thr324 GGRSREGtMELRTTP Homo sapiens
pmid sentence
Blt2 phosphorylation at thr355 by akt is necessary for blt2-mediated chemotaxis.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation TKT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-265102 Thr382 GCATRNRtVPFCSTF Homo sapiens HeLa Cell
pmid sentence
Akt phosphorylates TKT on Thr382, markedly enhancing enzyme activity and increasing carbon flow through the nonoxidative PPP, thereby increasing purine synthesis.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation CDK2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244176 Thr39 LKKIRLDtETEGVPS Homo sapiens
pmid sentence
Akt phosphorylates cdk2 at threonine 39 residue both in vitro and in vivo. Although cdk2 threonine 39 phosphorylation mediated by akt enhances cyclin-a binding, it is dispensable for its basal binding and the kinase activity.
Publications: 1 Organism: Homo Sapiens
+ PPP2CAdown-regulates img/direct_inhibition.png dephosphorylation AKT 0.89
Identifier Residue Sequence Organism Cell Line
SIGNOR-114767 Thr450 TAQMITItPPDQDDS Homo sapiens
pmid sentence
Integrin alpha 2 beta 1 promotes activation of protein phosphatase 2a and dephosphorylation of akt and glycogen synthase kinase 3 beta
Publications: 1 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network, PI3K/AKT Signaling
+ AKTdown-regulates img/direct_inhibition.png phosphorylation SKI 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-188969 Thr458 QPRKRKLtVDTPGAP Homo sapiens
pmid sentence
The phosphorylation of ski at threonine 458 is induced by akt pathway activators including insulin, insulin-like growth factor-1, and hepatocyte growth factor. The phosphorylation of ski causes its destabilization and reduces ski-mediated inhibition of expression of another negative regulator of tgf-beta, smad7
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation HK2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-267576 Thr473 QHRARQKtLEHLQLS Homo sapiens Cardiomyocyte Cell Line
pmid sentence
Hexokinase II is phosphorylated by Akt leading to increased mitochondrial binding and mitochondrial protection.We found that Akt, activated by receptor agonists, translocates to mitochondria and phosphorylates HK-II at Thr473, a critical step in Akt-mediated mitoHK-II increase and protection in cardiomyocytes
Identifier Residue Sequence Organism Cell Line
SIGNOR-259985 Homo sapiens Nasopharyngeal Carcinoma Cell
pmid sentence
K63-linked ubiquitination enhances the interaction between Akt and HK2 and eventually increases HK2 phosphorylation on Thr473 and mitochondrial localization
Publications: 2 Organism: Homo Sapiens
Pathways:Glycolysis and Gluconeogenesis
+ AKTup-regulates img/direct-activation.png phosphorylation SRPK2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244341 Thr492 PSHDRSRtVSASSTG Homo sapiens Neuron
pmid sentence
Here we show that srpk2, a protein kinase specific for the serine/arginine (sr) family of splicing factors, triggers cell cycle progression in neurons and induces apoptosis through regulation of nuclear cyclin d1. Akt phosphorylates srpk2 on thr-492 and promotes its nuclear translocation leading to cyclin d1 up-regulation, cell cycle reentry, and neuronal apoptosis.
Publications: 1 Organism: Homo Sapiens
Tissue: Brain
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation ADARB1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-266358 Thr553 LQGERLLtMSCSDKI in vitro
pmid sentence
AKT-dependent phosphorylation of the adenosine deaminases ADAR-1 and -2 inhibits deaminase activity. Using site-directed mutagenesis of suspected AKT phosphorylation sites, AKT was found to primarily phosphorylate ADAR1p110 and ADAR2 on T738 and T553, respectively, and overexpression of the phosphomimic mutants ADAR1p110 (T738D) and ADAR2 (T553D) resulted in a 50-100% reduction in editase activity.
Publications: 1 Organism: In Vitro
+ AKTup-regulates img/direct-activation.png phosphorylation EZR 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244263 Thr567 QGRDKYKtLRQIRQG Homo sapiens
pmid sentence
Purified akt directly phosphorylates recombinant ezrin at threonine 567 in vitro in an atp-dependent manner. ezrin activation after initiation of na+-glucose cotransport requires akt2 expression
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation WNK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-139391 Thr60 EYRRRRHtMDKDSRG Homo sapiens
pmid sentence
Phosphorylation of wnk1 on thr-58 contributes to sgk1 activation. these data suggest that activation of sgk1 by wnk1 requires the catalytic activity of akt.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates activity img/direct-activation.png phosphorylation PIK3R6 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-275723 Thr607 SHRPREVtVSLRATG
pmid sentence
P84 forms a negative regulatory complex with p110gamma to control PI3Kgamma signalling during cell migration|However, phosphorylation at this site was confirmed using an in vitro kinase assay in which Akt kinase was shown to readily phosphorylate Thr607 using a p84 peptide (Figure 1e), where Thr607 in conjunction with surrounding residues forms an Akt kinase consensus sequence|In contrast, although p84-T607A exhibited basal p110γ dimerisation, this interaction could not be further induced with stimulation
Publications: 1
+ AKTup-regulates activity img/direct-activation.png phosphorylation RPS3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-259816 Thr70 GRRIRELtAVVQKRF Rattus norvegicus PC12-AC Cell
pmid sentence
Here, we show that human RPS3 is a physiological target of Akt kinase and a novel mediator of neuronal apoptosis. NGF stimulation resulted in phosphorylation of threonine 70 of RPS3 by Akt, and this phosphorylation was required for Akt binding to RPS3.our experiment demonstrated that Akt up-regulates the endonuclease activity of RPS3 via phosphorylation and led us to believe that Akt phosphorylation of RPS3 after DNA damage is an antiapoptotic signal or a molecular switch that extends the life of a cell after DNA damage.
Publications: 1 Organism: Rattus Norvegicus
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation ITGB3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-251479 Thr779 LYKEATStFTNITYR in vitro
pmid sentence
Threonine phosphorylation of the beta 3 integrin cytoplasmic tail, at a site recognized by PDK1 and Akt/PKB in vitro, regulates Shc binding.|We recently observed that phosphorylation of a threonine (Thr-753), six amino acids proximal to tyrosine 759 in beta(3) of the platelet specific integrin alpha(IIb)beta(3), inhibits outside-in signaling through this receptor.| A survey of several protein kinases revealed that Thr-753 was avidly phosphorylated by PDK1 and Akt/PKB in vitro.
Publications: 1 Organism: In Vitro
Pathways:Integrin Signaling
+ AKTdown-regulates img/direct_inhibition.png phosphorylation TAL1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-137942 Thr90 EARHRVPtTELCRPP Homo sapiens T-lymphocyte, Leukemia Cell
pmid sentence
Akt phosphorylates tal1 oncoprotein and inhibits its repressor activity. / our results show that akt specifically phosphorylates thr90 of the tal1 protein within its transactivation domain in vitro and in vivo.
Publications: 1 Organism: Homo Sapiens
+ TNK2up-regulates activity img/direct-activation.png phosphorylation AKT 0.437
Identifier Residue Sequence Organism Cell Line
SIGNOR-252457 Tyr176 EKATGRYyAMKILKK Mus musculus
pmid sentence
Ack1 (also known as ACK or TNK2), which directly phosphorylates AKT at an evolutionarily conserved tyrosine 176 in the kinase domain. Tyr176-phosphorylated AKT localizes to the plasma membrane and promotes Thr308/Ser473-phosphorylation leading to AKT activation.
Publications: 1 Organism: Mus Musculus
Tissue: Prostate Adenocarcinoma Cell
+ PTK6up-regulates img/direct-activation.png phosphorylation AKT 0.454
Identifier Residue Sequence Organism Cell Line
SIGNOR-138437 Tyr315 TFCGTPEyLAPEVLE Homo sapiens
pmid sentence
These observations suggest that RET/PTC is able to phosphorylate the Y315 residue of PKB, an event that results in maximal activation of PKB for RET/PTC-induced thyroid tumorigenesis.
Identifier Residue Sequence Organism Cell Line
SIGNOR-166506 Tyr315 TFCGTPEyLAPEVLE Homo sapiens Breast Cancer Cell
pmid sentence
Here we demonstrate that AKT is a direct substrate of PTK6 and that AKT tyrosine residues 315 and 326 are phosphorylated by PTK6.
Identifier Residue Sequence Organism Cell Line
SIGNOR-166510 Tyr326 EVLEDNDyGRAVDWW Homo sapiens Breast Cancer Cell
pmid sentence
Here we demonstrate that AKT is a direct substrate of PTK6 and that AKT tyrosine residues 315 and 326 are phosphorylated by PTK6.
Publications: 3 Organism: Homo Sapiens
Tissue: Prostate Gland
+ SRCup-regulates activity img/direct-activation.png phosphorylation AKT 0.664
Identifier Residue Sequence Organism Cell Line
SIGNOR-246368 Tyr315 TFCGTPEyLAPEVLE Chlorocebus aethiops COS Cell
pmid sentence
Regulation of Akt/PKB Activation by Tyrosine PhosphorylationAs shown in Fig. 2 d, while mutation of Tyr340 has little effect on either tyrosine phosphorylation or kinase activity of Akt induced by Src527F, substitution of Tyr315 or Tyr326 with a phenylalanine, respectively, dramatically reduces both the tyrosine phosphorylation and kinase activity of Akt. The combination of these two mutations abolishes Src-induced tyrosine phosphorylation of Akt as well as its kinase activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-246373 Tyr315 TFCGTPEyLAPEVLE Homo sapiens HEK-293 Cell
pmid sentence
We also showed that phosphorylation of Tyr-315 in Akt induced by Src or EGF is dependent on the integrity of this proline-rich motif. Furthermore, the Akt mutant lacking this proline motif fails to block the transcription activity of Forkhead in 293 cells and poorly stimulates the proliferation of Madin-Darby canine kidney cells. Taken together, our data suggest that the interaction between the SH3 domain of Src family kinases and the proline-rich motif in the C-terminal regulatory region of Akt is required for tyrosine phosphorylation of Akt and its subsequent activation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-246377 Tyr326 EVLEDNDyGRAVDWW Chlorocebus aethiops COS Cell
pmid sentence
Regulation of Akt/PKB Activation by Tyrosine PhosphorylationAs shown in Fig. 2 d, while mutation of Tyr340 has little effect on either tyrosine phosphorylation or kinase activity of Akt induced by Src527F, substitution of Tyr315 or Tyr326 with a phenylalanine, respectively, dramatically reduces both the tyrosine phosphorylation and kinase activity of Akt. The combination of these two mutations abolishes Src-induced tyrosine phosphorylation of Akt as well as its kinase activity.
Publications: 3 Organism: Chlorocebus Aethiops, Homo Sapiens
Pathways:EGFR Signaling, Integrin Signaling, Rhabdomyosarcoma
+ RETup-regulates img/direct-activation.png phosphorylation AKT 0.328
Identifier Residue Sequence Organism Cell Line
SIGNOR-166514 Tyr315 TFCGTPEyLAPEVLE Homo sapiens
pmid sentence
The PKB Y315 residue, which is known to be phosphorylated by Src tyrosine kinase, was also a major site of phosphorylation by RET/PTC. RET/PTC-mediated tyrosine phosphorylation results in the activation of PKB kinase activity
Identifier Residue Sequence Organism Cell Line
SIGNOR-244443 Homo sapiens
pmid sentence
Overexpressed rai resulted in the potentiation of the ret-dependent activation of phosphatidylinositol 3-kinase (pi3k) and akt. The ret/ptc receptor tyrosine kinase that responds to glial cell-line-derived neurotrophic factor also phosphorylated akt tyrosine residue 315 promoting activation of akt
Publications: 2 Organism: Homo Sapiens
Pathways:Thyroid cancer
+ PPP1CAdown-regulates img/direct_inhibition.png dephosphorylation AKT 0.435
Identifier Residue Sequence Organism Cell Line
SIGNOR-244436 Homo sapiens
pmid sentence
Several stps have been reported to negatively regulate akt pathway. It has been shown that pp1 dephosphorylates akt and regulates cell survival.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png relocalization FOXO1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-252351 Mus musculus MEF Cell
pmid sentence
Akt1/PKBalpha was found to be the major regulator of phosphorylation and nuclear export of FoxO1, whose presence in the nucleus strongly attenuates adipocyte differentiation
Publications: 1 Organism: Mus Musculus
Pathways:FAP: Insulin-mediated adipogenesis, Insulin Signaling, Inhibition of Apoptosis, Leptin Signaling, Rhabdomyosarcoma, WNT/FLT3
+ THEM4down-regulates img/direct_inhibition.png binding AKT 0.687
Identifier Residue Sequence Organism Cell Line
SIGNOR-244455 Homo sapiens
pmid sentence
Here, we describe a protein partner for pkbalpha termed ctmp, or carboxyl-terminal modulator protein, that binds specifically to the carboxyl-terminal regulatory domain of pkbalpha at the plasma membrane. Binding of ctmp reduces the activity of pkbalpha by inhibiting phosphorylation on serine 473 and threonine 308.
Publications: 1 Organism: Homo Sapiens
+ Caspase 3 complexdown-regulates activity img/direct_inhibition.png cleavage AKT 0.66
Identifier Residue Sequence Organism Cell Line
SIGNOR-256446 in vitro
pmid sentence
P53 can inhibit the survival function of integrins by inducing the caspase-dependent cleavage and inactivation of the serine/threonine kinase akt/pkb;the involvement of caspase 3 in akt/pkb regulation was indicated by the ability of z-devd-fmk, a caspase 3 inhibitor, to block the alpha6beta4-associated reduction in akt/pkb levels in vivo, and by the ability of recombinant caspase 3 to promote the cleavage of akt/pkb in vitro
Publications: 1 Organism: In Vitro
+ PDPK1up-regulates img/direct-activation.png phosphorylation AKT 0.738
Identifier Residue Sequence Organism Cell Line
SIGNOR-244396 Homo sapiens
pmid sentence
Positive feedback involves mtorc2, which phosphorylates akt at serine 473, a phosphorylation required for maximum activation of akt in addition to phosphorylation at threonine 308 by pdk1.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, KIT in AML, miRNA in AML, AMPK Signaling, B-cell activation, EGFR Signaling, ErbB receptors in cancer, FAP: Insulin-mediated adipogenesis, FLT3-ITD signaling, Glioblastoma Multiforme, Hepatocellular Tumor, Insulin Signaling, Luminal Breast Cancer, mTOR in cancer, Malignant Melanoma, MTOR Signaling, Non-small-cell lung cancer (NSCLC), Prostate Cancer, PI3K/AKT Signaling, Rhabdomyosarcoma, RTKs in cancer, Thyroid cancer, T cell activation, VEGF Signaling
+ ANXA3up-regulates activity img/indirect-activation.png AKT 0.285
Identifier Residue Sequence Organism Cell Line
SIGNOR-262212 Homo sapiens
pmid sentence
We also investigated the potential regulation of cancer-associated signaling pathways by Anxa3 through screening for the altered expression of some common signaling molecules after Anxa3 downregulation. Decreased phosphorylation of MEK1/2, ERK1/2, Akt, and IκBα was detected after downregulating Anxa3 expression in A549 cells.
Publications: 1 Organism: Homo Sapiens
+ ACVR1down-regulates activity img/indirect_inhibition.png AKT 0.25
Identifier Residue Sequence Organism Cell Line
SIGNOR-243185 Homo sapiens
pmid sentence
Akt/mTOR signaling is a key target that accounts for myostatin function during muscle atrophy, uncovering a novel role for myostatin in protein metabolism and more specifically in the regulation of translation in skeletal muscle.
Publications: 1 Organism: Homo Sapiens
+ BRCA1down-regulates quantity by destabilization img/direct_inhibition.png ubiquitination AKT 0.52
Identifier Residue Sequence Organism Cell Line
SIGNOR-252458 Mus musculus MEF Cell
pmid sentence
The BRCA1-BRCT domains bind to phosphorylated AKT (pAKT) and lead to its ubiquitination toward protein degradation
Publications: 1 Organism: Mus Musculus
Pathways:Cell cycle: G2/M phase transition
+ IMPDH2up-regulates activity img/direct-activation.png binding AKT 0.394
Identifier Residue Sequence Organism Cell Line
SIGNOR-260961 Homo sapiens Colorectal Cancer Cell Line
pmid sentence
We further demonstrated that IMPDH2 overexpression accelerated G1/S phase cell cycle transition by inducing increased expression of cyclin D1 and Ki-67 and downregulation of p21Cip1 and p27Kip1. More importantly, G1/S phase cell cycle transition was triggered by IMPDH2 through activation of AKT activity, downregulation of mTOR and FOXO1 transcriptional activity. There is evidence that IMPDH2 interacts with the pleckstrin homology domain of PKB/AKT in the regulation of GTP biosynthesis.
Publications: 1 Organism: Homo Sapiens
+ SHHup-regulates img/indirect-activation.png AKT 0.481
Identifier Residue Sequence Organism Cell Line
SIGNOR-244446 Homo sapiens Myoblast, Satellite Cell
pmid sentence
Most importantly, we report that shh induces mapk/erk and phosphoinositide 3-kinase (pi3k)-dependent akt phosphorylation and that activation of both signaling pathways is essential for shh's signaling in muscle cells. However, the effect of shh on akt phosphorylation is more robust than that on mapk/erk, and data suggest that shh influences these pathways in a manner similar to igf-i.
Publications: 1 Organism: Homo Sapiens
Tissue: Muscle
+ AKTdown-regulates img/direct_inhibition.png binding SMAD3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-252345 Homo sapiens
pmid sentence
Pkb inhibits smad3 by preventing its phosphorylation, binding to smad4 and nuclear translocation. [...] Regulation of smad3 by pkb occurs through a kinase-activity-independent mechanism, resulting in a decrease in smad3-mediated transcription and protection of cells against tgf-beta-induced apoptosis.
Publications: 1 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network, Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, Hepatocellular Tumor, Pancreatic ductal adenocarcinoma (PDA), Thyroid Hormone Metabolism
+ PTENdown-regulates activity img/indirect_inhibition.png AKT 0.653
Identifier Residue Sequence Organism Cell Line
SIGNOR-166478 Homo sapiens Leukemia Cell
pmid sentence
Exposure of eol-1r cells to imatinib failed to dephosphorylate akt, erk and stat5, although pdgfralpha was effectively inactivated. The forced expression of pten negatively regulated these signal pathways and sensitized eol-1r cells to imatinib.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244439 Homo sapiens DU-145 Cell
pmid sentence
PTEN-mediated suppression of the PI3K/AKT pathway is well established, accumulating evidence suggests that nuclear PTEN also plays a critical role in tumor suppression
Publications: 2 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, B-cell activation, Glioblastoma Multiforme, Hepatocellular Tumor, Insulin Signaling, Luminal Breast Cancer, Malignant Melanoma, MTOR Signaling, Prostate Cancer, PI3K/AKT Signaling, Thyroid cancer
+ AKTup-regulates img/direct-activation.png binding HK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-148675 Homo sapiens
pmid sentence
The glucose dependence of the antiapoptotic effects of growth factors and akt plus a strong correlation between akt-regulated mitochondrial hexokinase association and apoptotic susceptibility suggest a major role for hexokinases in these effects.
Publications: 1 Organism: Homo Sapiens
Pathways:Glycolysis and Gluconeogenesis
+ FLT3up-regulates activity img/direct-activation.png phosphorylation AKT 0.441
Identifier Residue Sequence Organism Cell Line
SIGNOR-261522 Mus musculus BA/F3 Cell
pmid sentence
These data confirm previous findings that FLT3 receptors with ITD mutations efficiently trigger the activation of ERK, STAT5 and Akt in the absence of FL stimulation.
Publications: 1 Organism: Mus Musculus
Pathways:Acute Myeloid Leukemia, ASXL1 in AML, FLT3 in AML, miRNA in AML, AML_TRIPLETS, FLT3-ITD in AML, FLT3-ITD signaling, Triple mutant AML, NPM1_new, WNT/FLT3
+ AKTup-regulates activity img/indirect-activation.png CTNNB1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244225 Homo sapiens
pmid sentence
We show that pge2 stimulates colon cancer cell growth through its heterotrimeric guanine nucleotide-binding protein (g protein) coupled receptor, ep2, by a signaling route that involves the activation of phosphoinositide 3-kinase and the protein kinase akt by free g protein bg subunits and the direct association of the g protein as subunit with the regulator of g protein signaling (rgs) domain of axin. This leads to the inactivation and release of glycogen synthase kinase 3b from its complex with axin, thereby relieving the inhibitory phosphorylation of b-catenin and activating its signaling pathway.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, FLT3 in AML, FLT3-ITD in AML, FLT3-ITD signaling, Hepatocellular Tumor, Pancreatic ductal adenocarcinoma (PDA), Rhabdomyosarcoma, Thyroid cancer, WNT/FLT3
+ AKTup-regulates activity img/direct-activation.png phosphorylation mTORC1 0.719
Identifier Residue Sequence Organism Cell Line
SIGNOR-252817 Mus musculus 3T3-L1 Cell
pmid sentence
In examining the requirements for different Akt-mediated phosphorylation sites on TSC2, we find that only TSC2 mutants lacking all five previously identified Akt sites fully block insulin-stimulated mTORC1 signaling in reconstituted Tsc2 null cells, and this mutant also inhibits adipogenesis
Identifier Residue Sequence Organism Cell Line
SIGNOR-217586 Homo sapiens
pmid sentence
Pras40 is an insulin-regulated inhibitor of the mtorc1 protein kinase. Insulin stimulates akt/pkb-mediated phosphorylation of pras40, which prevents its inhibition of mtorc1 in cells and in vitro. Phosphorylation of pras40 on thr246 by pkb/akt facilitates efficient phosphorylation of ser183 by mtorc1.
Publications: 2 Organism: Mus Musculus, Homo Sapiens
Tissue: Muscle, Skeletal Muscle, Myotube
Pathways:AMPK Signaling, EGFR Signaling, Glioblastoma Multiforme, Insulin Signaling, Luminal Breast Cancer, mTOR in cancer, Pancreatic ductal adenocarcinoma (PDA), PI3K/AKT Signaling, Thyroid Hormone Metabolism, WNT/FLT3
+ FLT3up-regulates activity img/indirect-activation.png AKT 0.441
Identifier Residue Sequence Organism Cell Line
SIGNOR-245064 Homo sapiens
pmid sentence
We show that the presence of Flt3-ITD constitutively activates Akt (PKB), a key serine-threonine kinase within the phosphatidylinositol 3-kinase pathway.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, ASXL1 in AML, FLT3 in AML, miRNA in AML, AML_TRIPLETS, FLT3-ITD in AML, FLT3-ITD signaling, Triple mutant AML, NPM1_new, WNT/FLT3
+ AKTup-regulates activity img/direct-activation.png phosphorylation Hexokinase 0.496
Identifier Residue Sequence Organism Cell Line
SIGNOR-267577 Homo sapiens Cardiomyocyte Cell Line
pmid sentence
Earlier studies found that expression of active Akt increases mitochondrial HK activity and the anti-apoptotic effect of Akt required mitoHK
Publications: 1 Organism: Homo Sapiens
Pathways:Glycolysis and Gluconeogenesis
+ AKTup-regulates img/direct-activation.png phosphorylation SP7 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-174017 Homo sapiens
pmid sentence
We found that Akt phosphorylates Osterix and that Akt activation increases protein stability, osteogenic activity and transcriptional activity of Osterix. We also found that BMP-2 increases the protein level of Osterix in an Akt activity-dependent manner.
Publications: 1 Organism: Homo Sapiens
+ EML4-ALKup-regulates img/indirect-activation.png AKT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-253218 Homo sapiens
pmid sentence
A specific subtype of non-small cell lung cancer (NSCLC) characterized with an EML4-ALK fusion gene, which drives constitutive oncogenic activation of ALK kinase. We demonstrated that exogenous introduction of EML4-ALK protein with the substitution of lysine 1610 to an alanine in these two cell lines reduced the phosphorylation levels of AKT, one of downstream oncogenic molecules in the EML4-ALK pathway, and suppressed the growth of the two cell lines.
Publications: 1 Organism: Homo Sapiens
+ ZFATup-regulates quantity img/indirect-activation.png AKT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-261215 Mus musculus
pmid sentence
Ano6 deficiency significantly reduces ERK/AKT phosphorylation. In addition, Ano6-KD also affected levels of phosphorylated and total AKT levels.
Publications: 1 Organism: Mus Musculus
+ PTPN2down-regulates img/indirect_inhibition.png AKT 0.364
Identifier Residue Sequence Organism Cell Line
SIGNOR-98811 Homo sapiens
pmid sentence
We found that insulin-induced ir tyrosine phosphorylation and pkb/akt sig- naling were enhanced in tcptp- cells and suppressed upon tcptp reconstitution, providing persuasive evidence that tcptp can regulate ir activation and signaling.
Publications: 1 Organism: Homo Sapiens
+ FSTL1up-regulates activity img/indirect-activation.png AKT 0.391
Identifier Residue Sequence Organism Cell Line
SIGNOR-266604 Homo sapiens Endothelial Cell, Cardiac Muscle Fiber
pmid sentence
In this study, Fstl1 was shown to activate Akt signaling in cardiac myocytes and inhibit apoptosis. Thus, Fstl1 can function as a survival factor for both cardiac myocytes and endothelial cells via the activation of Akt signaling. 
Publications: 1 Organism: Homo Sapiens
+ MAPK14down-regulates activity img/indirect_inhibition.png AKT 0.631
Identifier Residue Sequence Organism Cell Line
SIGNOR-244461 Homo sapiens
pmid sentence
On the other hand, p38 alfa may negatively modulate akt activity, indipendently of pi3k by regulating the interaction between caveolin 1 and pp2a through a mechanism dependent on cell attachment.
Publications: 1 Organism: Homo Sapiens
Pathways:FLT3-ITD signaling
+ AKTup-regulates img/indirect-activation.png MTOR 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244314 Homo sapiens
pmid sentence
It was shown recently that akt activates mtor through direct phosphorylation of tsc2 the serine/threonine kinase akt is an upstream positive regulator of the mammalian target of rapamycin (mtor). However, the mechanism by which akt activates mtor is not fully understood. The known pathway by which akt activates mtor is via direct phosphorylation and tuberous sclerosis complex 2 (tsc2), which is a negative regulator of mtor.
Publications: 1 Organism: Homo Sapiens
Tissue: Muscle, Skeletal Muscle
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, KIT in AML, miRNA in AML, FLT3-ITD in AML, FLT3-ITD signaling, Glioblastoma Multiforme, Leptin Signaling, MTOR Signaling, Pancreatic ductal adenocarcinoma (PDA)
+ AKTup-regulates img/indirect-activation.png SLC2A4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-53968 Homo sapiens
pmid sentence
Akt is not only capable of stimulating the translocation of glut4 to the cell surface. Endogenous akt is likely to play a significant physiological role in insulin-stimulated glucose uptake in insulin targets such as muscle and adipose tissue
Identifier Residue Sequence Organism Cell Line
SIGNOR-45117 Homo sapiens
pmid sentence
The constitutively active Akt induced glucose uptake into adipocytes in the absence of insulin by stimulating translocation of the insulin-responsive glucose transporter 4 to the plasma membrane.
Publications: 2 Organism: Homo Sapiens
Pathways:Glycolysis and Gluconeogenesis, Insulin Signaling
+ CTLA4down-regulates activity img/indirect_inhibition.png AKT 0.589
Identifier Residue Sequence Organism Cell Line
SIGNOR-275409 Homo sapiens T-lymphocyte
pmid sentence
Akt phosphorylation in cells stimulated by CD3/CD28/CTLA-4 or CD3/CD28/PD-1 aAPCs did not have detectable phosphorylated Akt at any time point, indicating that CTLA-4 and PD-1 signaling blocked rather than delayed Akt activation.
Publications: 1 Organism: Homo Sapiens
+ HECW2up-regulates activity img/direct-activation.png binding AKT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-269459 Homo sapiens MCF-7 Cell
pmid sentence
NEDL2 acts as a scaffold protein to promote GDNF-stimulated Akt activation. biochemical analysis indicated that NEDL2 appears to act like a scaffold protein to recruit SHC, Grb2, PI3K (p110 and p85), PDK1 and Akt together to promote the signaling transduction. NEDL2 binds p85, p110 and Akt with different domains
Publications: 1 Organism: Homo Sapiens
+ 3-[1-[[4-(7-phenyl-3H-imidazo[4,5-g]quinoxalin-6-yl)phenyl]methyl]-4-piperidinyl]-1H-benzimidazol-2-onedown-regulates activity img/direct_inhibition.png chemical inhibition AKT 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-269849
pmid sentence
Different agents were used to inhibit either the PI3K/Akt or MEK/ERK pathways. The PI3K inhibitor, LY294002, and the Akt inhibitor, Akt inhibitor VIII, were used to inhibit the PI3K/Akt pathway.
Publications: 1
+ AKT img/unknown.png phosphorylation Histone H3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-265319 Homo sapiens
pmid sentence
Additionally, active akt1 kinase strongly phosphorylates histone h3 at serine 10 in vitro
Publications: 1 Organism: Homo Sapiens
+ DAB2IPdown-regulates activity img/direct_inhibition.png binding AKT 0.51
Identifier Residue Sequence Organism Cell Line
SIGNOR-254751 Homo sapiens
pmid sentence
DAB2IP inhibits the PI3K–AKT axis by directly interacting with both proteins, reducing phosphorylation and activation of AKT. The GAP activity of DAB2IP can further enforce inhibition of the PI3K–AKT axis by reducing Ras-dependent activation of PI3K p110α subunit.
Publications: 1 Organism: Homo Sapiens
+ POU5F1down-regulates quantity by repression img/direct_inhibition.png transcriptional regulation AKT 0.468
Identifier Residue Sequence Organism Cell Line
SIGNOR-242103 Homo sapiens NCCIT Cell
pmid sentence
Furthermore, in ECCs, unphosphorylated Oct4 bound to the AKT1 promoter and repressed its transcription.
Publications: 1 Organism: Homo Sapiens
+ BZW2up-regulates img/indirect-activation.png AKT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-261218 Homo sapiens Hepatobiliary Carcinoma Cell
pmid sentence
Overexpression (or silence) of BZW2 in HCC cells significantly stimulates (or decreases) the activation of the PI3K/AKT/mTOR signaling pathway
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates quantity by expression img/indirect-activation.png transcriptional regulation SLC2A1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-45064 Homo sapiens
pmid sentence
The constitutively active akt also increased the synthesis of the ubiquitously expressed glucose transporter 1. The increased glucose influx in the 3t3-l1 adipocytes directed lipid but not glycogen synthesis
Publications: 1 Organism: Homo Sapiens
Pathways:Glycolysis and Gluconeogenesis
+ AKTdown-regulates img/direct_inhibition.png phosphorylation FOXA2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-254978 Homo sapiens
pmid sentence
�Foxa-2 physically interacts with Akt, a key mediator of the phosphatidylinositol 3-kinase pathway and is phosphorylated at a single conserved site (T156) that is absent in Foxa-1 and Foxa-3 proteins. This Akt phosphorylation site in Foxa-2 is highly conserved from mammals to insects. Mutant Foxa-2T156A is resistant to Akt-mediated phosphorylation, nuclear exclusion, and transcriptional inactivation of Foxa-2-regulated gene expression.
Publications: 1 Organism: Homo Sapiens
Pathways:FAP: Insulin-mediated adipogenesis
+ MTCP1up-regulates img/direct-activation.png binding AKT 0.46
Identifier Residue Sequence Organism Cell Line
SIGNOR-244413 Homo sapiens T-lymphocyte, Leukemia Cell
pmid sentence
Full-length tcl1 and its isoforms bind to akt / in in vitro kinase assays using gsk-3_ as a substrate, we found that the presence of any of the tcl1 family proteins (tcl1, mtcp1, or tcl1b) as gst fusion proteins significantly enhanced akt-induced gsk-3_ phosphorylation
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/indirect_inhibition.png Apoptosis 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-260215 in vitro
pmid sentence
Multiple studies supporting the role of Akt in apoptosis suppression have connected Akt to cell death regulation either by demonstrating its downregulation following pro-apoptotic insults, or by using gene-transfer experiments that transduce both activated, anti-apoptotic and inactive, pro-apoptotic mutants of Akt.
Publications: 1 Organism: In Vitro
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, KIT in AML, miRNA in AML, AML_TRIPLETS, COVID-19 Causal Network, EBV infection, FLT3-ITD in AML, FLT3-ITD signaling, Inhibition of Apoptosis, Integrin Signaling, Malignant Melanoma, Triple mutant AML, NPM1_new, Pancreatic ductal adenocarcinoma (PDA), SARS-COV APOPTOSIS, Thyroid cancer
+ perifosinedown-regulates img/direct_inhibition.png chemical inhibition AKT 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-119189 Homo sapiens Prostate Gland Cancer Cell
pmid sentence
Perifosine, a novel alkylphospholipid, inhibits protein kinase B activation.| Our results demonstrate that Akt is an important cellular target of perifosine action. In addition, these studies show that the membrane translocation of certain PH domain-containing molecules can be greatly perturbed by the alkylphospholipid class of drugs and imply further that the PI3K/Akt pathway contributes to regulation of p21(WAF1/CIP1) expression.
Publications: 1 Organism: Homo Sapiens
+ NEU1down-regulates activity img/indirect_inhibition.png AKT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-260659 Homo sapiens YTS-1 Cell
pmid sentence
Taken together, these findings indicate that NEU1 overexpression reduces cell proliferation and enhances cell apoptosis through by downregulation of FN-integrin β1-mediated Akt signaling pathway.
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation STK3/4 0.406
Identifier Residue Sequence Organism Cell Line
SIGNOR-269944 Homo sapiens
pmid sentence
We determined that mst2 phosphorylation by akt limits mst2 activity in two ways: first, by blocking its binding to rassf1a and by promoting its association into the raf-1 inhibitory complex, and second, by preventing homodimerization of mst2, which is needed for its activation. we identified t117 and t384 as akt phosphorylation sites in mst2.
Publications: 1 Organism: Homo Sapiens
+ MK-2206down-regulates img/direct_inhibition.png chemical inhibition AKT 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-176043 Homo sapiens Skin Cancer Cell
pmid sentence
Treatment with the pi3k inhibitor ly294002 or the akt inhibitor mk2206 diminished s473 phosphorylation.
Publications: 1 Organism: Homo Sapiens
+ PIP3up-regulates activity img/direct-activation.png relocalization AKT 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-236509 Homo sapiens
pmid sentence
Binding of IGF to IGF-1R activates PI3K to generate PIP3 which in turn recruits and activates proteins that contain a pleckstrin homology ph) domain, including AKT and PDK1.
Identifier Residue Sequence Organism Cell Line
SIGNOR-236490 Homo sapiens
pmid sentence
Akt is a serine-threonine protein kinase that plays important roles in cell growth, proliferation and apoptosis. It is activated after binding to phosphatidylinositol phosphates pips) with phosphate groups at positions 3,4 and 3,4,5 on the inositol ring.
Publications: 2 Organism: Homo Sapiens
Tissue: Prostate Gland Cancer Cell
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, KIT in AML, miRNA in AML, AMPK Signaling, B-cell activation, EGFR Signaling, ErbB receptors in cancer, FAP: Insulin-mediated adipogenesis, FLT3-ITD signaling, Glioblastoma Multiforme, Hepatocellular Tumor, Insulin Signaling, mTOR in cancer, Malignant Melanoma, MTOR Signaling, Non-small-cell lung cancer (NSCLC), Prostate Cancer, Pancreatic ductal adenocarcinoma (PDA), PI3K/AKT Signaling, RTKs in cancer, Thyroid cancer, T cell activation, VEGF Signaling
+ AKTup-regulates img/direct-activation.png phosphorylation IKK-complex 0.645
Identifier Residue Sequence Organism Cell Line
SIGNOR-244281 Homo sapiens Thymoma Cell
pmid sentence
Although there are likely to be multiple levels of crosstalk between the pi3k-akt and nf-kb pathways, one mechanism has been attributed to direct phosphorylation of the amino acid residue t23 on ikb kinase alfa (ikkalfa) by akt, thereby leading to activation of this kinase upstream of nf-kb akt mediates ikkalpha phosphorylation at threonine 23 akt transiently associates in vivo with ikk and induces ikk activation. Akt mediates ikkalfa phosphorylation at threonine 23.Akt phosphorylates ikkalpha on t23, and this phosphorylation event is a prerequisite for the phosphorylation of p65 at s534 by ikkalpha and beta
Publications: 1 Organism: Homo Sapiens
Pathways:B-cell activation, COVID-19 Causal Network, EBV infection, FLT3-ITD signaling, Inflammosome Activation, Inhibition of Apoptosis, Pancreatic ductal adenocarcinoma (PDA), SARS-CoV INFLAMMATORY RESPONSE, T cell activation
+ AKTup-regulates img/indirect-activation.png PRKACA 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-145113 Homo sapiens Neuron
pmid sentence
Indicating that akt positively regulates shh signaling by controlling pka-mediated gli inactivation.
Publications: 1 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network, WNT/FLT3
+ PPP2CBdown-regulates activity img/direct_inhibition.png dephosphorylation AKT 0.488
Identifier Residue Sequence Organism Cell Line
SIGNOR-248614 Homo sapiens
pmid sentence
Overexpression of BTBD10 increased phosphorylation levels of Akts at both Thr(308) and Ser(473) while the reduction of the endogenous BTBD10 level resulted in a decrease in the phosphorylation levels of Akts. In vitro analysis indicated that BTBD10 bound to protein phosphatase 2A (PP2A) and inhibited dephosphorylation of Akts by PP2A.
Publications: 1 Organism: Homo Sapiens
+ PHLPP2down-regulates activity img/direct_inhibition.png dephosphorylation AKT 0.764
Identifier Residue Sequence Organism Cell Line
SIGNOR-248732 Homo sapiens Chronic Myeloid Leukemia Cell
pmid sentence
The Abl kinase inhibitors and depletion of Bcr-Abl induced the expression of PHLPP1 and PHLPP2, which dephosphorylated Ser-473 on Akt1, -2, and -3, resulting in inhibited proliferation of CML cells.|Thus, Bcr-Abl represses the expression of PHLPP1 and PHLPP2 and continuously activates Akt1, -2, and -3 via phosphorylation on Ser-473, resulting in the proliferation of CML cells.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/indirect-activation.png Survival 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-254952 Homo sapiens Mast Cell
pmid sentence
c-Kit promotes survival via PI3-kinase-dependent activation of Akt and phosphorylation of Bad, a pro-apoptotic molecule, at S136 in vivo.
Identifier Residue Sequence Organism Cell Line
SIGNOR-251550 Homo sapiens
pmid sentence
Akt promotes both cell growth and cell survival by inactivating its downstream substrates including GSK3, BAD, FOXO and TSC2.
Publications: 2 Organism: Homo Sapiens
Pathways:ErbB receptors in cancer, Glioblastoma Multiforme, Hepatocellular Tumor, Inhibition of Apoptosis, Luminal Breast Cancer, Malignant Melanoma, Non-small-cell lung cancer (NSCLC), Prostate Cancer, Pancreatic ductal adenocarcinoma (PDA), PI3K/AKT Signaling, Rhabdomyosarcoma, RTKs in cancer, Thyroid cancer
+ AKTup-regulates img/indirect-activation.png MEF2D 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244303 Homo sapiens Myoblast
pmid sentence
Two candidates that may function as mediators of pi3-k in the phosphorylation of mef2 proteins are pkb and big map kinase 1.
Publications: 1 Organism: Homo Sapiens
Tissue: Muscle, Skeletal Muscle
+ INPPL1down-regulates img/indirect_inhibition.png AKT 0.636
Identifier Residue Sequence Organism Cell Line
SIGNOR-244406 Homo sapiens B-lymphocyte
pmid sentence
Taken together, the data presented here demonstrate that ship inhibits akt primarily through regulation of akt membrane localization.
Publications: 1 Organism: Homo Sapiens
+ TNFup-regulates img/indirect-activation.png AKT 0.493
Identifier Residue Sequence Organism Cell Line
SIGNOR-244458 Homo sapiens
pmid sentence
Tumor necrosis factor (tnf) inhibited insulin-promoted tyrosine phosphorylation of irs-1 and activated the akt/protein kinase b serine-threonine kinase, a downstream target for phosphatidylinositol 3-kinase
Publications: 1 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network, EBV infection, Inflammosome Activation, Inhibition of Apoptosis, MTOR Signaling, SARS-COV APOPTOSIS, SARS-CoV INFLAMMATORY RESPONSE, Thyroid Hormone Metabolism
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation PRKAA1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-252740 in vitro
pmid sentence
It is proposed that the effect of insulin to antagonize AMP-activated protein kinase activation involves a hierarchical mechanism whereby Ser 485/Ser 491 phosphorylation by protein kinase B reduces subsequent phosphorylation of Thr 172 by LKB1 and the resulting activation of AMP-activated protein kinase.
Publications: 1 Organism: In Vitro
+ PIP3up-regulates img/direct-activation.png relocalization AKT 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-254982 Rattus norvegicus
pmid sentence
Activated hyperphosphorylated Akt-1 bound to Ptd Ins(3,4,5)P3 -containing vesicles in a similar manner to the inactive dephosphorylated enzyme
Publications: 1 Organism: Rattus Norvegicus
Pathways:Acute Myeloid Leukemia, BCR-ABL in AML, FLT3 in AML, KIT in AML, miRNA in AML, AMPK Signaling, B-cell activation, EGFR Signaling, ErbB receptors in cancer, FAP: Insulin-mediated adipogenesis, FLT3-ITD signaling, Glioblastoma Multiforme, Hepatocellular Tumor, Insulin Signaling, mTOR in cancer, Malignant Melanoma, MTOR Signaling, Non-small-cell lung cancer (NSCLC), Prostate Cancer, Pancreatic ductal adenocarcinoma (PDA), PI3K/AKT Signaling, RTKs in cancer, Thyroid cancer, T cell activation, VEGF Signaling
+ MIPOL1down-regulates activity img/indirect_inhibition.png AKT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-261135 Homo sapiens
pmid sentence
In our results, we also showed that re-expression of MIPOL1 is associated with suppression phosphorylation of AKT and p65, a subunit of NF-ҡB. This suggests that MIPOL1 may inhibit invasion by suppression of phosphorylation of AKT and p65 to further inhibit the expression of MMP-9
Publications: 1 Organism: Homo Sapiens
+ PDCD1down-regulates activity img/indirect_inhibition.png AKT 0.35
Identifier Residue Sequence Organism Cell Line
SIGNOR-275408 Homo sapiens T-lymphocyte
pmid sentence
Akt phosphorylation in cells stimulated by CD3/CD28/CTLA-4 or CD3/CD28/PD-1 aAPCs did not have detectable phosphorylated Akt at any time point, indicating that CTLA-4 and PD-1 signaling blocked rather than delayed Akt activation.
Publications: 1 Organism: Homo Sapiens
+ TTC3down-regulates quantity by destabilization img/direct_inhibition.png ubiquitination AKT 0.404
Identifier Residue Sequence Organism Cell Line
SIGNOR-252459 Mus musculus NIH-3T3 Cell
pmid sentence
TTC3 is an Akt-specific E3 ligase that binds to phosphorylated Akt and facilitates its ubiquitination and degradation within the nucleus
Publications: 1 Organism: Mus Musculus
+ Mdown-regulates activity img/indirect_inhibition.png AKT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-260200 Homo sapiens HEK-293FT Cell
pmid sentence
Consistent with our previous result, we detected a reduced phosphorylated PKB/Akt level and diminished PKB/Akt activity in mammalian cells expressing M-protein.
Publications: 1 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network, SARS-COV APOPTOSIS, SARS-CoV INFLAMMATORY RESPONSE
+ INSup-regulates img/indirect-activation.png AKT 0.744
Identifier Residue Sequence Organism Cell Line
SIGNOR-97392 Mus musculus
pmid sentence
The forkhead transcription factor foxo1 is regulated by insulin via akt-dependent phosphorylation and nuclear exclusion.
Publications: 1 Organism: Mus Musculus
Pathways:AMPK Signaling, FAP: Insulin-mediated adipogenesis, Insulin Signaling, Luminal Breast Cancer, Leptin Signaling, mTOR in cancer, MTOR Signaling
+ PF-04691502down-regulates img/direct_inhibition.png chemical inhibition AKT 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-205977 Homo sapiens
pmid sentence
Publications: 1 Organism: Homo Sapiens
+ PP2CA_R1A_R2A down-regulates activity img/direct_inhibition.png dephosphorylation AKT 0.671
Identifier Residue Sequence Organism Cell Line
SIGNOR-243530 Mus musculus NIH-3T3 Cell
pmid sentence
Regulation of phosphorylation of Thr-308 of Akt, cell proliferation, and survival by the B55alpha regulatory subunit targeting of the protein phosphatase 2A holoenzyme to Akt.|Phosphorylation of Akt at regulatory residues Thr-308 and Ser-473 leads to its full activation. The protein phosphatase 2A (PP2A) has long been known to negatively regulate Akt activity. The PP2A holoenzyme consists of the structural subunit (A), catalytic subunit (C), and a variable regulatory subunit (B).
Publications: 1 Organism: Mus Musculus
+ TCL1Aup-regulates img/direct-activation.png binding AKT 0.823
Identifier Residue Sequence Organism Cell Line
SIGNOR-244449 Homo sapiens T-lymphocyte, Leukemia Cell
pmid sentence
Full-length tcl1 and its isoforms bind to akt / in in vitro kinase assays using gsk-3_ as a substrate, we found that the presence of any of the tcl1 family proteins (tcl1, mtcp1, or tcl1b) as gst fusion proteins significantly enhanced akt-induced gsk-3_ phosphorylation
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/indirect-activation.png Proliferation 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-257606 Homo sapiens
pmid sentence
Activation of PDGFRα stimulates proliferation of PDGFRα(+) cells through PI3K-Akt and MEK2-MAPK signaling pathways, and aberrant accumulation of PDGFRα(+) cells was conspicuous in muscles of patients with both genetic and non-genetic muscle diseases.
Identifier Residue Sequence Organism Cell Line
SIGNOR-254353 Mus musculus
pmid sentence
Protein kinase B (PKB/Akt) is an important modulator of insulin signaling, cell proliferation, and survival. Using small interfering RNA duplexes in nontransformed mammalian cells, we show that only Akt1 is essential for cell proliferation
Publications: 2 Organism: Homo Sapiens, Mus Musculus
Pathways:Acute Myeloid Leukemia, ASXL1 in AML, BCR-ABL in AML, FLT3 in AML, KIT in AML, miRNA in AML, AML_TRIPLETS, AMPK Signaling, B-cell activation, COVID-19 Causal Network, EGFR Signaling, EBV infection, ErbB receptors in cancer, FLT3-ITD in AML, FLT3-ITD signaling, Glioblastoma Multiforme, Hepatocellular Tumor, Insulin Signaling, Integrin Signaling, Luminal Breast Cancer, Leptin Signaling, mTOR in cancer, Malignant Melanoma, Triple mutant AML, NPM1_new, Non-small-cell lung cancer (NSCLC), Prostate Cancer, Pancreatic ductal adenocarcinoma (PDA), PI3K/AKT Signaling, Rhabdomyosarcoma, RTKs in cancer, Thyroid cancer, VEGF Signaling, WNT/FLT3
+ PDPK2Pup-regulates img/direct-activation.png phosphorylation AKT 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-129965 Homo sapiens
pmid sentence
Akt to the plasma membrane where it is phosphorylated and activated by phosphoinositide-dependent kinase (pdk) 1 and pdk2.
Publications: 1 Organism: Homo Sapiens
+ AKTup-regulates img/direct-activation.png phosphorylation DLX5 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-173976 Homo sapiens
pmid sentence
Akt, a member of the ser-ine/threonine-specific protein kinase, was found to phosphorylate osx and dlx5. akt interacts with and phosphorylates dlx5. In addition, we provide evidences that akt kinase activity is important for akt to enhance the protein stability and transcriptional activity of dlx5.
Identifier Residue Sequence Organism Cell Line
SIGNOR-244228 Homo sapiens
pmid sentence
Akt, a member of the ser-ine/threonine-specific protein kinase, was found to phosphorylate osx and dlx5. akt interacts with and phosphorylates dlx5. In addition, we provide evidences that akt kinase activity is important for akt to enhance the protein stability and transcriptional activity of dlx5.
Publications: 2 Organism: Homo Sapiens
+ AKTdown-regulates img/indirect_inhibition.png FAS 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-123239 Homo sapiens
pmid sentence
Akt may serve to stimulate certain proteins (e.g., Ikk) involved in the prevention of apoptosis such as nf-kb as well as repress other proteins normally involved in the induction of apoptosis such as the forkhead transcription factors (fkhr, now know as foxo3), creb, glycogen synthetase-3 kinase-beta (gsk-3beta), fas, caspase-9 and cell cycle inhibitors such as p27
Publications: 1 Organism: Homo Sapiens
Pathways:COVID-19 Causal Network, SARS-COV APOPTOSIS
+ Amyloid_fibril_formationdown-regulates img/indirect_inhibition.png AKT 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-255493
pmid sentence
Excessive accumulation of Aβ protein in the AD brain may lead to a decrease in the levels of phosphatidylinositol-3 kinase (PI3K) and the serine/threonine protein kinase B (Akt) activity.
Publications: 1
+ PIK3C3up-regulates img/indirect-activation.png AKT 0.445
Identifier Residue Sequence Organism Cell Line
SIGNOR-75376 Homo sapiens
pmid sentence
Pkb is activated through recruitment to cellular membranes by pi3k lipid products and phosphorylation by 3h-phosphoinositide-dependent kinase-1 (pdk1)
Publications: 1 Organism: Homo Sapiens
+ TRIB3down-regulates activity img/direct_inhibition.png binding AKT 0.604
Identifier Residue Sequence Organism Cell Line
SIGNOR-237850 Homo sapiens HEK-293 Cell
pmid sentence
TRB3 expression is induced in liver under fasting conditions, and TRB3 disrupts insulin signaling by binding directly to Akt and blocking activation of the kinase.
Publications: 1 Organism: Homo Sapiens
Tissue: Liver
+ PI3Kup-regulates activity img/direct-activation.png phosphorylation AKT 0.784
Identifier Residue Sequence Organism Cell Line
SIGNOR-254950 Homo sapiens
pmid sentence
C-Kit promotes survival via PI3-kinase-dependent activation of Akt and phosphorylation of Bad, a pro-apoptotic molecule, at S136 in vivo.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, ASXL1 in AML, BCR-ABL in AML, FLT3 in AML, KIT in AML, B-cell activation, COVID-19 Causal Network, EBV infection, ErbB receptors in cancer, FAP: Insulin-mediated adipogenesis, FLT3-ITD in AML, FLT3-ITD signaling, Hepatocellular Tumor, Inflammosome Activation, Integrin Signaling, Leptin Signaling, mTOR in cancer, Non-small-cell lung cancer (NSCLC), Pancreatic ductal adenocarcinoma (PDA), PI3K/AKT Signaling, RTKs in cancer, SARS-CoV INFLAMMATORY RESPONSE, T cell activation, Thyroid Hormone Metabolism, VEGF Signaling, WNT/FLT3
+ ITLN1up-regulates activity img/indirect-activation.png AKT 0.318
Identifier Residue Sequence Organism Cell Line
SIGNOR-272499 Homo sapiens
pmid sentence
ITLN1 increases Akt phosphorylation in adipocytes, osteoblasts, and mesenchymal cells. |n mesenchymal stem cells, ITLN1 also leads to Akt-mediated proliferation, resistance to oxidative stress, and secretion of proangiogenic factors
Publications: 1 Organism: Homo Sapiens
+ MSTNdown-regulates activity img/indirect_inhibition.png AKT 0.503
Identifier Residue Sequence Organism Cell Line
SIGNOR-255339 Homo sapiens
pmid sentence
In the current study, it was demonstrated that myostatin inhibits activation of Akt, in both myoblasts and myotubes.
Publications: 1 Organism: Homo Sapiens
+ PP1down-regulates activity img/direct_inhibition.png dephosphorylation AKT 0.452
Identifier Residue Sequence Organism Cell Line
SIGNOR-264659 Homo sapiens
pmid sentence
Here, we identify PP1 as a serine/threonine phosphatase that associates with and dephosphorylates AKT in breast cancer cells|The heat shock protein 90 inhibitor geldanamycin and the ErbB inhibitor ZD1839 promote rapid PP1 phosphatase-dependent inactivation of AKT in ErbB2 overexpressing breast cancer cells
Publications: 1 Organism: Homo Sapiens
+ ID1up-regulates img/direct-activation.png binding AKT 0.34
Identifier Residue Sequence Organism Cell Line
SIGNOR-255942 Homo sapiens Kasumi-1 Cell
pmid sentence
We have determined that Id1 physically interacts with AKT1, through its C-terminal region, and promotes AKT1 phosphorylation;
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, FLT3 in AML, miRNA in AML
+ MAPK14up-regulates activity img/indirect-activation.png AKT 0.631
Identifier Residue Sequence Organism Cell Line
SIGNOR-244465 Homo sapiens
pmid sentence
We show [] that the kinase activity and s473 phosphorylation of akt induced by lpa and s1p requires both mitogen-activated protein (map) kinase kinase (mek) and p38 map kinase. [] among different stimuli tested, platelet-derived growth factor stimulates s473 phosphorylation of akt in a mek- and p38-dependent manner. However, epidermal growth factor, thrombin, and endothelin-1?stimulated Akt s473 phosphorylation require p38 but not mek.
Publications: 1 Organism: Homo Sapiens
Pathways:FLT3-ITD signaling
+ ATMdown-regulates activity img/indirect_inhibition.png AKT 0.459
Identifier Residue Sequence Organism Cell Line
SIGNOR-244392 Homo sapiens
pmid sentence
The decreased atm expression suggests that atm is involved in the development of insulin resistance through down-regulation of akt activity.
Publications: 1 Organism: Homo Sapiens
Tissue: Muscle
Pathways:FLT3-ITD signaling, Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition
+ AKTup-regulates activity img/direct-activation.png phosphorylation ELK1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-259029 Homo sapiens
pmid sentence
Both mitogen-activated protein kinase (MAPK)/extracellular-signal-regulated kinases (ERK) 1/2 and phosphatidylinositide-3-OH kinase (PI3K)/Akt pathways regulate activation of E-twenty-six (ETS)-like transcription factor 1 (Elk-1) in U138 glioblastoma cells. The phosphatidylinositide-3-OH kinase (PI3K)/Akt pathway was also involved in the Elk-1 activation. Activation of the Elk-1 led to an increased survival and a proliferative response with the EGF stimulation in the U138 glioblastoma cells.
Publications: 1 Organism: Homo Sapiens
Pathways:EGFR Signaling, Glioblastoma Multiforme, Integrin Signaling
+ PI3Kup-regulates activity img/indirect-activation.png AKT 0.784
Identifier Residue Sequence Organism Cell Line
SIGNOR-252703 Homo sapiens
pmid sentence
However, here we show through phosphoprotein profiling and functional genomic studies that many PIK3CA mutant cancer cell lines and human breast tumors exhibit only minimal AKT activation and a diminished reliance on AKT for anchorage-independent growth
Identifier Residue Sequence Organism Cell Line
SIGNOR-252715 Homo sapiens
pmid sentence
PKB induction requires phosphorylation of two critical residues, threonine 308 in the activation loop and serine 473 near the carboxyl terminus. Membrane localization of PKB was found to be a primary determinant of serine 473 phosphorylation. PI3K activity was equally important for promoting phosphorylation of serine 473,
Publications: 2 Organism: Homo Sapiens
Tissue: Breast Cancer Cell
Pathways:Acute Myeloid Leukemia, ASXL1 in AML, BCR-ABL in AML, FLT3 in AML, KIT in AML, B-cell activation, COVID-19 Causal Network, EBV infection, ErbB receptors in cancer, FAP: Insulin-mediated adipogenesis, FLT3-ITD in AML, FLT3-ITD signaling, Hepatocellular Tumor, Inflammosome Activation, Integrin Signaling, Leptin Signaling, mTOR in cancer, Non-small-cell lung cancer (NSCLC), Pancreatic ductal adenocarcinoma (PDA), PI3K/AKT Signaling, RTKs in cancer, SARS-CoV INFLAMMATORY RESPONSE, T cell activation, Thyroid Hormone Metabolism, VEGF Signaling, WNT/FLT3
+ TCL1Bup-regulates img/direct-activation.png binding AKT 0.662
Identifier Residue Sequence Organism Cell Line
SIGNOR-244452 Homo sapiens T-lymphocyte, Leukemia Cell
pmid sentence
In vivo, tcl1 forms trimers, which associate with akt. Tcl1 facilitates the oligomerization and activation of akt. Our data show that tcl1 is a novel akt kinase coactivator, which promotes akt-induced cell survival and proliferation.
Publications: 1 Organism: Homo Sapiens
+ PPP2CBdown-regulates img/direct_inhibition.png dephosphorylation AKT 0.488
Identifier Residue Sequence Organism Cell Line
SIGNOR-42050 Homo sapiens
pmid sentence
These results confirm that the activity changes observed are achieved by a reversible phosphorylation mechanism, and also argue that pp2a may negatively regulate rac-pk activity in vivo. Dephosphorylation of the activated rac-pk in itro by pp2ac resulted in an 87% reduction of kinase activity
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation GSK3B/Axin/APC 0.545
Identifier Residue Sequence Organism Cell Line
SIGNOR-227952 Homo sapiens
pmid sentence
We show that PGE2 stimulates colon cancer cell growth through its heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptor, EP2, by a signaling route that involves the activation of phosphoinositide 3-kinase and the protein kinase Akt by free G protein betagamma subunits and the direct association of the G protein alphas subunit with the regulator of G protein signaling (RGS) domain of axin. This leads to the inactivation and release of glycogen synthase kinase 3beta from its complex with axin, thereby relieving the inhibitory phosphorylation of beta-catenin and activating its signaling pathway.
Publications: 1 Organism: Homo Sapiens
Pathways:Hepatocellular Tumor
+ PTPN1down-regulates img/direct_inhibition.png dephosphorylation AKT 0.732
Identifier Residue Sequence Organism Cell Line
SIGNOR-132959 Homo sapiens
pmid sentence
Whereas insulin-induced phosphatidylinositol 3-kinase/akt signaling was prolonged in both tcptp-/- and ptp1b-/- immortalized mouse embryo fibroblasts (mefs), mitogen-activated protein kinase erk1/2 signaling was elevated only in ptp1b- mefs
Publications: 1 Organism: Homo Sapiens
Pathways:Insulin Signaling
+ FHITdown-regulates img/indirect_inhibition.png AKT 0.38
Identifier Residue Sequence Organism Cell Line
SIGNOR-143700 Homo sapiens Lung Cancer Cell
pmid sentence
Fhit inhibited activity of akt, a key effector in the phosphatidylinositol 3-oh kinase (pi3k) pathway;loss of endogenous fhit expression caused increased akt activity in vitro and in vivo, and overexpression of constitutively active akt inhibited fhit-induced apoptosis
Publications: 1 Organism: Homo Sapiens
Pathways:Pancreatic ductal adenocarcinoma (PDA)
+ PHLPP1down-regulates activity img/direct_inhibition.png dephosphorylation AKT 0.751
Identifier Residue Sequence Organism Cell Line
SIGNOR-248331 Homo sapiens Chronic Myeloid Leukemia Cell
pmid sentence
The Abl kinase inhibitors and depletion of Bcr-Abl induced the expression of PHLPP1 and PHLPP2, which dephosphorylated Ser-473 on Akt1, -2, and -3, resulting in inhibited proliferation of CML cells.|Thus, Bcr-Abl represses the expression of PHLPP1 and PHLPP2 and continuously activates Akt1, -2, and -3 via phosphorylation on Ser-473, resulting in the proliferation of CML cells.
Publications: 1 Organism: Homo Sapiens
+ PIK3CAup-regulates activity img/indirect-activation.png AKT 0.81
Identifier Residue Sequence Organism Cell Line
SIGNOR-236436 Homo sapiens
pmid sentence
However, here we show through phosphoprotein profiling and functional genomic studies that many PIK3CA mutant cancer cell lines and human breast tumors exhibit only minimal AKT activation and a diminished reliance on AKT for anchorage-independent growth
Publications: 1 Organism: Homo Sapiens
Tissue: Breast Cancer Cell
Pathways:AMPK Signaling, EGFR Signaling, Glioblastoma Multiforme, Luminal Breast Cancer, mTOR in cancer, Malignant Melanoma, MTOR Signaling, Prostate Cancer, Rhabdomyosarcoma
+ PIK3C3up-regulates img/direct-activation.png relocalization AKT 0.445
Identifier Residue Sequence Organism Cell Line
SIGNOR-75370 Homo sapiens
pmid sentence
One of the best characterized targets of pi3k lipid products is the protein kinase akt or protein kinase b (pkb). In quiescent cells, pkb resides in the cytosol in a low-activity conformation. Upon cellular stimulation, pkb is activated through recruitment to cellular membranes by pi3k lipid products and phosphorylation by 3h-phosphoinositide-dependent kinase-1 (pdk1).
Publications: 1 Organism: Homo Sapiens
+ GFsup-regulates activity img/indirect-activation.png AKT 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-245411 Homo sapiens
pmid sentence
Akt normally resides in the cytosol under serum-starved conditions, but translocates to the plasma membrane where it is subsequently phosphorylated and activated in response to growth factor treatment. Phosphorylation of Akt at Thr308 by phosphoinositide-dependent kinase-1 (PDK1) and at Ser473 by mammalian target of rapamycin (mTOR) complex 2 (mTORC2) is required for full Akt activation
Publications: 1 Organism: Homo Sapiens
Pathways:Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, RTKs in cancer
+ AKTdown-regulates activity img/indirect_inhibition.png MAPK14 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244288 Homo sapiens Breast Cancer Cell
pmid sentence
Our data indicate that akt2 inhibits cisplatin-induced jnk/p38 and bax activation through phosphorylation of ask1
Publications: 1 Organism: Homo Sapiens
Pathways:FLT3-ITD signaling
+ sirolimusup-regulates img/indirect-activation.png AKT 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-144156 Homo sapiens
pmid sentence
We now show that mtor inhibition induces insulin receptor substrate-1 expression and abrogates feedback the pathway, resulting in akt activation both in cancer cell lines and in patient tumors treated with the rapamycin derivative, rad001.
Publications: 1 Organism: Homo Sapiens
Pathways:FLT3-ITD signaling
+ AKTdown-regulates activity img/indirect_inhibition.png INSIG2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-256212 Mus musculus
pmid sentence
MTORC1 activation is not sufficient to stimulate hepatic SREBP1c in the absence of Akt signaling, revealing the existence of an additional downstream pathway also required for this induction. We provide evidence that this mTORC1-independent pathway involves Akt-mediated suppression of Insig2a, a liver-specific transcript encoding the SREBP1c inhibitor INSIG2.
Publications: 1 Organism: Mus Musculus
Tissue: Liver
+ AKTdown-regulates activity img/direct_inhibition.png binding CDKN1A 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-244187 Homo sapiens Myoblast
pmid sentence
More importantly, the consequences of phosphorylation of either Thr145 or Ser146 are distinct. When p21 is phosphorylated on Thr145, it localizes to the nucleus and results in the disruption of the association between proliferating cell nuclear antigen and p21. Furthermore, phosphorylation of Thr145 promotes stabilization of p21
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, FLT3-ITD in AML, FLT3-ITD signaling, Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, Rhabdomyosarcoma
+ GSK690693down-regulates img/direct_inhibition.png chemical inhibition AKT 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-193000 Homo sapiens
pmid sentence
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates img/direct_inhibition.png phosphorylation TBC1D4 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-99300 Homo sapiens
pmid sentence
Recently, we identified a 160-kda protein in adipocytes, designated as160, that is phosphorylated by the insulin-activated kinase akt
Publications: 1 Organism: Homo Sapiens
Tissue: Muscle
+ AKTdown-regulates img/direct_inhibition.png binding CDKN1B 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-200875 Homo sapiens
pmid sentence
Furthermore, akt promotes cell cycle progression through downregulation of the cyclin dependent kinase inhibitor p27kip1.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, Integrin Signaling
+ AKTdown-regulates img/direct_inhibition.png binding PHB2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-234441 Mus musculus
pmid sentence
Akt binds prohibitin 2 and relieves its repression of myod and muscle differentiation
Publications: 1 Organism: Mus Musculus
+ AKTdown-regulates activity img/direct_inhibition.png phosphorylation TSC 0.777
Identifier Residue Sequence Organism Cell Line
SIGNOR-251526 Mus musculus 3T3-L1 Cell
pmid sentence
In examining the requirements for different Akt-mediated phosphorylation sites on TSC2, we find that only TSC2 mutants lacking all five previously identified Akt sites fully block insulin-stimulated mTORC1 signaling in reconstituted Tsc2 null cells, and this mutant also inhibits adipogenesis
Publications: 1 Organism: Mus Musculus
Pathways:AMPK Signaling, Insulin Signaling, Luminal Breast Cancer, mTOR in cancer, MTOR Signaling
+ PPP2R2Adown-regulates activity img/direct_inhibition.png binding AKT 0.474
Identifier Residue Sequence Organism Cell Line
SIGNOR-243526 Mus musculus NIH-3T3 Cell
pmid sentence
Regulation of phosphorylation of Thr-308 of Akt, cell proliferation, and survival by the B55alpha regulatory subunit targeting of the protein phosphatase 2A holoenzyme to Akt.|Phosphorylation of Akt at regulatory residues Thr-308 and Ser-473 leads to its full activation. The protein phosphatase 2A (PP2A) has long been known to negatively regulate Akt activity. The PP2A holoenzyme consists of the structural |Here we report the identification of the specific B regulatory subunit that targets the PP2A holoenzyme to Akt. We found endogenous association of PP2A AB55C holoenzymes with Akt by co-immunoprecipitation analyses in pro-lymphoid FL5.12 cells.subunit (A), catalytic subunit (C), and a variable regulatory subunit (B).
Publications: 1 Organism: Mus Musculus
+ PIK3CAup-regulates activity img/direct-activation.png phosphorylation AKT 0.81
Identifier Residue Sequence Organism Cell Line
SIGNOR-244429 Homo sapiens
pmid sentence
PKB induction requires phosphorylation of two critical residues, threonine 308 in the activation loop and serine 473 near the carboxyl terminus. Membrane localization of PKB was found to be a primary determinant of serine 473 phosphorylation. PI3K activity was equally important for promoting phosphorylation of serine 473,
Publications: 1 Organism: Homo Sapiens
Pathways:AMPK Signaling, EGFR Signaling, Glioblastoma Multiforme, Luminal Breast Cancer, mTOR in cancer, Malignant Melanoma, MTOR Signaling, Prostate Cancer, Rhabdomyosarcoma
+ NKX3-1down-regulates activity img/direct_inhibition.png AKT 0.503
Identifier Residue Sequence Organism Cell Line
SIGNOR-251552 Homo sapiens
pmid sentence
NKX3.1 negatively regulates AKT activity in an AR-dependent manner
Publications: 1 Organism: Homo Sapiens
Pathways:Prostate Cancer
+ CASP3down-regulates activity img/direct_inhibition.png cleavage AKT 0.66
Identifier Residue Sequence Organism Cell Line
SIGNOR-72677 in vitro
pmid sentence
P53 can inhibit the survival function of integrins by inducing the caspase-dependent cleavage and inactivation of the serine/threonine kinase akt/pkb;the involvement of caspase 3 in akt/pkb regulation was indicated by the ability of z-devd-fmk, a caspase 3 inhibitor, to block the alpha6beta4-associated reduction in akt/pkb levels in vivo, and by the ability of recombinant caspase 3 to promote the cleavage of akt/pkb in vitro
Publications: 1 Organism: In Vitro
Pathways:COVID-19 Causal Network, Inhibition of Apoptosis, SARS-COV APOPTOSIS
+ ACP1down-regulates activity img/direct_inhibition.png dephosphorylation AKT 0.327
Identifier Residue Sequence Organism Cell Line
SIGNOR-248458 Mus musculus
pmid sentence
Reduction in the levels of both LMW-PTP isoforms in vitro and in vivo increased tyrosine phosphorylation of IR and AktSer473 and increased IRS-1- and IRS-2-associated PI3-K activities in both liver and fat.|Activated PI3-K stimulates Akt (or protein kinase B) that in turn phosphorylates and inactivates glycogen synthase kinase-3
Publications: 1 Organism: Mus Musculus
+ AKTdown-regulates img/direct_inhibition.png phosphorylation GSK3B 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-255488
pmid sentence
AKT phosphorylates and inhibits GSK3 in addition to many other substrates including TSC2, FOXO proteins, TBC1D4.
Publications: 1
Pathways:Acute Myeloid Leukemia, AML_TRIPLETS, FLT3-ITD signaling, Cell cycle: G1/S phase transition, Cell cycle: G2/M phase transition, Insulin Signaling, MTOR Signaling, Triple mutant AML, PI3K/AKT Signaling, WNT/FLT3
+ AKTdown-regulates img/direct_inhibition.png phosphorylation FOXO6 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-66032 Homo sapiens
pmid sentence
The phosphorylation of the two remaining akt-dependent sites inhibits foxo6 transcriptional activity
Publications: 1 Organism: Homo Sapiens
+ AKTdown-regulates quantity by repression img/indirect_inhibition.png INSIG2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-256211 Mus musculus
pmid sentence
MTORC1 activation is not sufficient to stimulate hepatic SREBP1c in the absence of Akt signaling, revealing the existence of an additional downstream pathway also required for this induction. We provide evidence that this mTORC1-independent pathway involves Akt-mediated suppression of Insig2a, a liver-specific transcript encoding the SREBP1c inhibitor INSIG2.
Publications: 1 Organism: Mus Musculus
+ IGF1Rup-regulates activity img/indirect-activation.png AKT 0.397
Identifier Residue Sequence Organism Cell Line
SIGNOR-244403 Mus musculus C2C12 Cell
pmid sentence
we show that IGF-1 unexpectedly acts via Akt to antagonize calcineurin signalling during myotube hypertrophy.
Publications: 1 Organism: Mus Musculus
Pathways:EBV infection, Hepatocellular Tumor, Non-small-cell lung cancer (NSCLC), Rhabdomyosarcoma
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