Relation Results

Summary

Name APC-c
Primary ID SIGNOR-C150
Links CPX-1860
Type complex
Formed by ANAPC2, CDC27, ANAPC1, CDC23, CDC16, ANAPC7, ANAPC10, ANAPC4, ANAPC13, ANAPC5, CDC26, ANAPC11
Relations 52

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Type: Score: Layout: SPV 
0.8730.2050.20.70.3950.2810.2660.3170.5540.9190.90.2620.8770.3420.310.8290.20.4370.20.8710.8470.250.4860.290.920.2940.30.8460.3290.9130.8810.8360.4220.9170.20.3750.6080.540.6570.7860.2490.2590.2910.8880.860.421CDC20APC-cPNNPFKFB3Mitotic_checkpointCDH1KIF2CANLNPPM1DPTTG1CDC16ANAPC4CLSPNANAPC1REV1PHF8ANAPC13IRS2CCNFPIWIL1ANAPC2FZR1TK1CDC6KIF4AANAPC5USP37KIF18ACDC26SKILCDC23ANAPC7AURKAANAPC10CDR2FBXW5CCNB1CCNA2UBE2SMCCMOAP1KIFC1DTYMKCDC27ANAPC11PSMD4

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Relations
Regulator
Mechanism
target
score ℹ
+ CDC20up-regulates activity img/direct-activation.png binding APC-c 0.873
Identifier Residue Sequence Organism Cell Line
SIGNOR-272896 Homo sapiens HEK-293 Cell
pmid sentence
 Here, we show that human REV1 undergoes proteosomal degradation mediated by the E3 ubiquitin ligase known as anaphase-promoting complex (APC). REV1 associates with APC. Overexpression of APC coactivator CDH1 or CDC20 promotes polyubiquitination and proteosomal degradation of REV1.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252014
pmid sentence
In addition to E2 enzymes, APC/C activity is also strictly dependent on one of several co-activator proteins that associate with APC/C during specific periods of the cell cycle. The best studied of these are Cdc20 and Cdh1
Identifier Residue Sequence Organism Cell Line
SIGNOR-272880 Homo sapiens HEK-293 Cell
pmid sentence
 We showed that PHF8 interacts with the CDC20-containing APC (APC(cdc20)) primarily during mitosis. we demonstrate that mutations of the LXPKXLF motif abrogate polyubiquitylation of PHF8 by the APC. APC substrates are typically cell cycle regulators, and consistent with this, the loss of PHF8 leads to prolonged G2 phase and defective mitosis.
Publications: 3 Organism: Homo Sapiens,
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination PNN 0.205
Identifier Residue Sequence Organism Cell Line
SIGNOR-272201 Homo sapiens Pancreatic Ductal Adenocarcinoma Cell
pmid sentence
Apo-PIWIL1 functions as a co-activator for APC/C ubiquitin ligase. Here, we show that in the absence of piRNAs, human PIWIL1 in PDAC functions as an oncoprotein by activating the anaphase promoting complex/cyclosome (APC/C) E3 complex, which then targets a critical cell adhesion-related protein, Pinin, to enhance PDAC metastasis.
Publications: 1 Organism: Homo Sapiens
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination PFKFB3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-271435 Homo sapiens SH-SY5Y Cell
pmid sentence
We have recently discovered that the glycolysis-promoting enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, isoform 3 (PFKFB3), is degraded by the E3 ubiquitin ligase APC/C-Cdh1, which also degrades cell-cycle proteins.
Publications: 1 Organism: Homo Sapiens
+ APC-cup-regulates img/indirect-activation.png Mitotic_checkpoint 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-265978 Homo sapiens
pmid sentence
We then found that the joint action of TRIP13 and p31comet also promotes MCC disassembly, releases APC/C from checkpoint inhibition, and inactivates the mitotic checkpoint.
Publications: 1 Organism: Homo Sapiens
+ CDH1up-regulates activity img/direct-activation.png binding APC-c 0.395
Identifier Residue Sequence Organism Cell Line
SIGNOR-274058
pmid sentence
In early mitosis, APC/C is activated through binding to Cdc20, and in late M, Cdc20 is replaced by Cdh1, the second activator of APC/C.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination KIF2C 0.281
Identifier Residue Sequence Organism Cell Line
SIGNOR-266111 in vitro
pmid sentence
Biochemical studies on the kinesins confirmed KIFC1, KIF18A, KIF2C, and KIF4A as APC/C substrates. Furthermore, we showed that the APC/CCDH1-dependent degradation of KIFC1 regulates the bipolar spindle formation and proper cell division. Our in vitro degradation assays showed a time-dependent degradation for four of the five potential substrates tested: KIF18A, KIF2C, KIFC1 and KIF4A were readily degraded in vitro, however remained stable in the presence of either APC/C inhibitor (Fig​(Fig4A4A and Supplementary Fig S3A).
Identifier Residue Sequence Organism Cell Line
SIGNOR-276864 Homo sapiens HeLa Cell
pmid sentence
Our studies suggest new mechanisms by which Plk1 regulates MCAK: the degradation of MCAK is controlled by Plk1 phosphorylation on S621, whereas its activity is modulated by Plk1 phosphorylation on S632/S633 in mitosis.We have recently shown that S621 in MCAK is the major phosphorylation site of Plk1, which is responsible for regulating MCAK's degradation by promoting the association of MCAK with APC/CCdc20.  In the present study, we have addressed another two residues phosphorylated by Plk1, namely S632/S633 in the C-terminus of MCAK. Our data suggest that Plk1 phosphorylates S632/S633 and regulates its catalytic activity in mitosis. This phosphorylation is required for proper spindle assembly during early phases of mitosis.
Publications: 2 Organism: In Vitro, Homo Sapiens
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination ANLN 0.266
Identifier Residue Sequence Organism Cell Line
SIGNOR-272655 Homo sapiens HeLa Cell
pmid sentence
 Ubiquitination of anillin required a destruction-box and was mediated by Cdh1, an activator of APC/C. Overexpression of Cdh1 reduced the levels of anillin, whereas inactivation of APC/C(Cdh1) increased the half-life of anillin.
Publications: 1 Organism: Homo Sapiens
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination PPM1D 0.317
Identifier Residue Sequence Organism Cell Line
SIGNOR-275492
pmid sentence
Phosphorylation of multiple residues in the catalytic domain of PPM1D during mitosis, including Ser40 by Cyclin-dependent kinase 1 (CDK1), leads to ubiquitination of PPM1D and subsequent proteasomal degradation by Adenomatous polyposis coli (APC) and cell-division cycle protein 20 (CDC20)
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination PTTG1 0.554
Identifier Residue Sequence Organism Cell Line
SIGNOR-265049
pmid sentence
Complexed with the activator proteins CDC20 or CDH1 (Fang et al., 1998, Visintin et al., 1997), the APC/C recognizes, ubiquitinates, and targets for proteasomal degradation a multitude of cell cycle regulators containing KEN or D box degrons, including securin, cyclin A, and cyclin B.
Publications: 1
+ CDC16form complex img/form-complex.png binding APC-c 0.919
Identifier Residue Sequence Organism Cell Line
SIGNOR-252006
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ ANAPC4form complex img/form-complex.png binding APC-c 0.9
Identifier Residue Sequence Organism Cell Line
SIGNOR-252004
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination CLSPN 0.262
Identifier Residue Sequence Organism Cell Line
SIGNOR-274056
pmid sentence
Claspin Is Degraded in G0 and G1 via the APC/CCdh1 Ubiquitin Ligase
Publications: 1
+ ANAPC1form complex img/form-complex.png binding APC-c 0.877
Identifier Residue Sequence Organism Cell Line
SIGNOR-252001
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination REV1 0.342
Identifier Residue Sequence Organism Cell Line
SIGNOR-272895 Homo sapiens HEK-293 Cell
pmid sentence
 Here, we show that human REV1 undergoes proteosomal degradation mediated by the E3 ubiquitin ligase known as anaphase-promoting complex (APC). REV1 associates with APC. Overexpression of APC coactivator CDH1 or CDC20 promotes polyubiquitination and proteosomal degradation of REV1.
Identifier Residue Sequence Organism Cell Line
SIGNOR-272894 Homo sapiens HEK-293 Cell
pmid sentence
 Here, we show that human REV1 undergoes proteosomal degradation mediated by the E3 ubiquitin ligase known as anaphase-promoting complex (APC). REV1 associates with APC. Overexpression of APC coactivator CDH1 or CDC20 promotes polyubiquitination and proteosomal degradation of REV1.
Publications: 2 Organism: Homo Sapiens
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination PHF8 0.31
Identifier Residue Sequence Organism Cell Line
SIGNOR-272881 Homo sapiens HEK-293 Cell
pmid sentence
 We showed that PHF8 interacts with the CDC20-containing APC (APC(cdc20)) primarily during mitosis. we demonstrate that mutations of the LXPKXLF motif abrogate polyubiquitylation of PHF8 by the APC. APC substrates are typically cell cycle regulators, and consistent with this, the loss of PHF8 leads to prolonged G2 phase and defective mitosis.
Publications: 1 Organism: Homo Sapiens
+ ANAPC13form complex img/form-complex.png binding APC-c 0.829
Identifier Residue Sequence Organism Cell Line
SIGNOR-252012
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination IRS2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272196 Homo sapiens HeLa Cell
pmid sentence
We conducted an unbiased proteomic screen to uncover novel substrates of the Anaphase Promoting Complex/Cyclosome (APC/C), a ubiquitin ligase that controls the abundance of key cell cycle regulators. We found that IRS2 levels are regulated by APC/C activity and that IRS2 is a direct APC/C target in G1 Consistent with the APC/C's role in degrading cell cycle regulators. Consistent with this observation, we found that APC/C inhibition decreased the polyubiquitylation of HA-tagged IRS2 in HeLa cells treated with MG132 (Fig. 2G).
Publications: 1 Organism: Homo Sapiens
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination CCNF 0.437
Identifier Residue Sequence Organism Cell Line
SIGNOR-266362 Homo sapiens
pmid sentence
We show that cyclin F, a cell-cycle-regulated substrate receptor (F-box protein) for the SCF, is targeted for degradation by APC/C. Furthermore, we establish that Cdh1 is itself a substrate of SCF(cyclin F). Cyclin F loss impairs Cdh1 degradation and delays S-phase entry, and this delay is reversed by simultaneous removal of Cdh1.
Publications: 1 Organism: Homo Sapiens
+ PIWIL1up-regulates img/direct-activation.png binding APC-c 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-272202 Homo sapiens Pancreatic Ductal Adenocarcinoma Cell
pmid sentence
PIWIL1 leads to the constitutive activation of APC/C in PDAC cells. Here, we show that in the absence of piRNAs, human PIWIL1 in PDAC functions as an oncoprotein by activating the anaphase promoting complex/cyclosome (APC/C) E3 complex, which then targets a critical cell adhesion-related protein, Pinin, to enhance PDAC metastasis. Collectively, these results demonstrate that Pinin is an authentic substrate of APC/CPIWIL1 in PDAC cells.
Publications: 1 Organism: Homo Sapiens
+ ANAPC2form complex img/form-complex.png binding APC-c 0.871
Identifier Residue Sequence Organism Cell Line
SIGNOR-252002
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ FZR1up-regulates activity img/direct-activation.png binding APC-c 0.847
Identifier Residue Sequence Organism Cell Line
SIGNOR-252015
pmid sentence
In addition to E2 enzymes, APC/C activity is also strictly dependent on one of several co-activator proteins that associate with APC/C during specific periods of the cell cycle. The best studied of these are Cdc20 and Cdh1
Identifier Residue Sequence Organism Cell Line
SIGNOR-272897 Homo sapiens HEK-293 Cell
pmid sentence
 Here, we show that human REV1 undergoes proteosomal degradation mediated by the E3 ubiquitin ligase known as anaphase-promoting complex (APC). REV1 associates with APC. Overexpression of APC coactivator CDH1 or CDC20 promotes polyubiquitination and proteosomal degradation of REV1.
Identifier Residue Sequence Organism Cell Line
SIGNOR-272611 Chlorocebus aethiops COS Cell
pmid sentence
We previously showed that human Aurora-A is turned over through the anaphase promoting complex/cyclosome (APC/C)–ubiquitin–proteasome pathway. The association of two distinct WD40 repeat proteins known as Cdc20 and Cdh1, respectively, sequentially activates the APC/C. The present study shows that Aurora-A degradation is dependent on hCdh1 in vivo, not on hCdc20, and that Aurora-A is targeted for proteolysis through distinct structural features of the destruction box, the KEN box motifs and its kinase activity.
Publications: 3 Organism: , Homo Sapiens, Chlorocebus Aethiops
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination TK1 0.25
Identifier Residue Sequence Organism Cell Line
SIGNOR-272946 in vitro
pmid sentence
We show that hTK1 is degraded via a ubiquitin-proteasome pathway in mammalian cells and that anaphase-promoting complex/cyclosome (APC/C) activator Cdh1 is not only a necessary but also a rate-limiting factor for mitotic degradation of hTK1. By in vitro ubiquitinylation assays, we demonstrated that hTK1 is targeted for degradation by the APC/C-Cdh1 ubiquitin ligase dependent on this KEN box motif.
Publications: 1 Organism: In Vitro
+ APC-cup-regulates activity img/direct-activation.png binding CDC6 0.486
Identifier Residue Sequence Organism Cell Line
SIGNOR-271386 Homo sapiens HeLa Cell
pmid sentence
Furthermore, APC, in association with CDH1, ubiquitinates CDC6 in vitro, and both APC and CDH1 are required and limiting for CDC6 proteolysis in vivo.
Publications: 1 Organism: Homo Sapiens
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination KIF4A 0.29
Identifier Residue Sequence Organism Cell Line
SIGNOR-266112 in vitro
pmid sentence
Biochemical studies on the kinesins confirmed KIFC1, KIF18A, KIF2C, and KIF4A as APC/C substrates. Furthermore, we showed that the APC/CCDH1-dependent degradation of KIFC1 regulates the bipolar spindle formation and proper cell division. Our in vitro degradation assays showed a time-dependent degradation for four of the five potential substrates tested: KIF18A, KIF2C, KIFC1 and KIF4A were readily degraded in vitro, however remained stable in the presence of either APC/C inhibitor (Fig​(Fig4A4A and Supplementary Fig S3A).
Publications: 1 Organism: In Vitro
+ ANAPC5form complex img/form-complex.png binding APC-c 0.92
Identifier Residue Sequence Organism Cell Line
SIGNOR-252005
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination USP37 0.294
Identifier Residue Sequence Organism Cell Line
SIGNOR-265048 Homo sapiens HEK-293 Cell
pmid sentence
USP37 is ubiquitinated by APCCDH1 in late mitosis and early G1 phase||Inactive USP37 now becomes a substrate for APCCDH1, undergoing K11-linked polyubiquitination and proteasomal degradation. Elimination of USP37 ensures that it does not antagonize APC/C substrate degradation during mitosis.
Publications: 1 Organism: Homo Sapiens
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination KIF18A 0.3
Identifier Residue Sequence Organism Cell Line
SIGNOR-266110 in vitro
pmid sentence
Biochemical studies on the kinesins confirmed KIFC1, KIF18A, KIF2C, and KIF4A as APC/C substrates. Furthermore, we showed that the APC/CCDH1-dependent degradation of KIFC1 regulates the bipolar spindle formation and proper cell division. Our in vitro degradation assays showed a time-dependent degradation for four of the five potential substrates tested: KIF18A, KIF2C, KIFC1 and KIF4A were readily degraded in vitro, however remained stable in the presence of either APC/C inhibitor (Fig​(Fig4A4A and Supplementary Fig S3A).
Publications: 1 Organism: In Vitro
+ CDC26form complex img/form-complex.png binding APC-c 0.846
Identifier Residue Sequence Organism Cell Line
SIGNOR-252011
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination SKIL 0.329
Identifier Residue Sequence Organism Cell Line
SIGNOR-272622 Homo sapiens HEK-293 Cell
pmid sentence
 We demonstrate that the anaphase-promoting complex (APC) is a ubiquitin ligase required for the destruction of SnoN and that the APC pathway is regulated by TGF-beta. The destruction box of SnoN is required for its degradation in response to TGF-beta signaling. Furthermore, the APC activator CDH1 and Smad3 synergistically regulate SnoN degradation. Under these circumstances, CDH1 forms a quaternary complex with SnoN, Smad3, and APC. 
Publications: 1 Organism: Homo Sapiens
+ CDC23form complex img/form-complex.png binding APC-c 0.913
Identifier Residue Sequence Organism Cell Line
SIGNOR-252008
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination APC-c 0.881
Identifier Residue Sequence Organism Cell Line
SIGNOR-272725 Homo sapiens HEK-293 Cell
pmid sentence
Cdc20 is a co-activator of the anaphase-promoting complex/cyclosome (APC/C complex), which recruits substrates at particular phases of the cell cycle and mediates their degradation. Overexpression of Cdc20 resulted in decreased levels of both endogenous Sp100 protein and overexpressed Sp100 mRNA in HEK 293 cells. Our results suggested that sp100 is a novel substrate of Cdc20 and it is degraded by the ubiquitination pathway. The intact D-box of Sp100 was necessary for this process.
Publications: 1 Organism: Homo Sapiens
+ ANAPC7form complex img/form-complex.png binding APC-c 0.836
Identifier Residue Sequence Organism Cell Line
SIGNOR-252007
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination AURKA 0.422
Identifier Residue Sequence Organism Cell Line
SIGNOR-272612 Chlorocebus aethiops COS Cell
pmid sentence
We previously showed that human Aurora-A is turned over through the anaphase promoting complex/cyclosome (APC/C)–ubiquitin–proteasome pathway. The association of two distinct WD40 repeat proteins known as Cdc20 and Cdh1, respectively, sequentially activates the APC/C. The present study shows that Aurora-A degradation is dependent on hCdh1 in vivo, not on hCdc20, and that Aurora-A is targeted for proteolysis through distinct structural features of the destruction box, the KEN box motifs and its kinase activity.
Publications: 1 Organism: Chlorocebus Aethiops
+ ANAPC10form complex img/form-complex.png binding APC-c 0.917
Identifier Residue Sequence Organism Cell Line
SIGNOR-252009
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination CDR2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-252024
pmid sentence
Here we find that cdr2 is cell cycle regulated in tumor cells with protein levels peaking in mitosis. As cells exit mitosis, cdr2 is ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) and rapidly degraded by the proteasome. Previously we showed that cdr2 binds to the oncogene c-myc, and here we extend this observation to show that cdr2 and c-myc interact to synergistically regulate c-myc-dependent transcription during passage through mitosis.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination FBXW5 0.375
Identifier Residue Sequence Organism Cell Line
SIGNOR-275477
pmid sentence
We show that FBXW5 levels are controlled by the anaphase-promoting (APC/C) complex, which targets FBXW5 for degradation during mitosis and G1, thereby helping to reset the centrosome duplication machinery.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination CCNB1 0.608
Identifier Residue Sequence Organism Cell Line
SIGNOR-265051
pmid sentence
Complexed with the activator proteins CDC20 or CDH1 (Fang et al., 1998, Visintin et al., 1997), the APC/C recognizes, ubiquitinates, and targets for proteasomal degradation a multitude of cell cycle regulators containing KEN or D box degrons, including securin, cyclin A, and cyclin B.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination CCNA2 0.54
Identifier Residue Sequence Organism Cell Line
SIGNOR-265050
pmid sentence
Complexed with the activator proteins CDC20 or CDH1 (Fang et al., 1998, Visintin et al., 1997), the APC/C recognizes, ubiquitinates, and targets for proteasomal degradation a multitude of cell cycle regulators containing KEN or D box degrons, including securin, cyclin A, and cyclin B.
Publications: 1
+ UBE2Sup-regulates activity img/direct-activation.png binding APC-c 0.657
Identifier Residue Sequence Organism Cell Line
SIGNOR-265080 Homo sapiens HeLa Cell
pmid sentence
Here, we identify the highly conserved Ube2S as a regulator of human and Drosophila APC/C. Ube2S functions as a K11-specific chain elongating E2 of APC/C, which depends on chain initiation by UbcH10. Together, UbcH10 and Ube2S are required for the degradation of all APC/C substrates tested so far, spindle formation, and progression of cells through mitosis.
Publications: 1 Organism: Homo Sapiens
+ MCCdown-regulates activity img/direct_inhibition.png binding APC-c 0.786
Identifier Residue Sequence Organism Cell Line
SIGNOR-265977 Homo sapiens HeLa Cell
pmid sentence
The mitotic (or spindle assembly) checkpoint system delays anaphase until all chromosomes are correctly attached to the mitotic spindle. When the checkpoint is active, a Mitotic Checkpoint Complex (MCC) assembles and inhibits the ubiquitin ligase Anaphase-Promoting Complex/Cyclosome (APC/C). MCC is composed of the checkpoint proteins Mad2, BubR1, and Bub3 associated with the APC/C activator Cdc20.
Publications: 1 Organism: Homo Sapiens
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination MOAP1 0.249
Identifier Residue Sequence Organism Cell Line
SIGNOR-272912 Homo sapiens HeLa Cell
pmid sentence
MOAP-1 is an APC/CCdh1 substrate. Here, we identify MOAP-1 as a novel APC/CCdh1 substrate. MOAP-1 is degraded during G1 by APC/CCdh1, and this degradation is inhibited by Trim39 acting on the APC/C.
Publications: 1 Organism: Homo Sapiens
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png ubiquitination KIFC1 0.259
Identifier Residue Sequence Organism Cell Line
SIGNOR-266109 in vitro
pmid sentence
Biochemical studies on the kinesins confirmed KIFC1, KIF18A, KIF2C, and KIF4A as APC/C substrates. Furthermore, we showed that the APC/CCDH1-dependent degradation of KIFC1 regulates the bipolar spindle formation and proper cell division. Our in vitro degradation assays showed a time-dependent degradation for four of the five potential substrates tested: KIF18A, KIF2C, KIFC1 and KIF4A were readily degraded in vitro, however remained stable in the presence of either APC/C inhibitor (Fig​(Fig4A4A and Supplementary Fig S3A).
Publications: 1 Organism: In Vitro
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination DTYMK 0.291
Identifier Residue Sequence Organism Cell Line
SIGNOR-272653 Homo sapiens HeLa Cell
pmid sentence
We demonstrate that TMPK is recognized and degraded by APC/C-Cdc20/Cdh1-mediated pathways from mitosis to the early G1 phase, whereas TK1 is targeted for degradation by APC/C-Cdh1 after mitotic exit. 
Publications: 1 Organism: Homo Sapiens
+ CDC27form complex img/form-complex.png binding APC-c 0.888
Identifier Residue Sequence Organism Cell Line
SIGNOR-252003
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ ANAPC11form complex img/form-complex.png binding APC-c 0.86
Identifier Residue Sequence Organism Cell Line
SIGNOR-252010
pmid sentence
The anaphase promoting complex/cyclosome (APC/C) is a ubiquitin ligase that has essential functions in and outside the eukaryotic cell cycle. It is the most complex molecular machine that is known to catalyse ubiquitylation reactions, and it contains more than a dozen subunits that assemble into a large 1.5-MDa complex.
Publications: 1
+ APC-cdown-regulates quantity by destabilization img/direct_inhibition.png polyubiquitination PSMD4 0.421
Identifier Residue Sequence Organism Cell Line
SIGNOR-272750 in vitro
pmid sentence
S5a/Rpn10 is a ubiquitin (Ub)-binding protein that is a subunit of the 26S proteasome but also exists free in the cytosol. It binds poly-Ub chains through its two Ub-interacting motifs (UIMs). We discovered that, unlike typical substrates of Ub ligases (E3s), S5a can be ubiquitinated by all E3s tested including multimeric and monomeric Ring finger E3s (MuRF1, Siah2, Parkin, APC, and SCF(betaTRCP1)), the U-box E3, CHIP, and HECT domain E3s (E6AP and Nedd4) when assayed with UbcH5 or related Ub-conjugating enzymes.The short half-life of S5a presumably is because of the presence of the UIM domain and reflects the ubiquitination of free S5a by many E3s.
Publications: 1 Organism: In Vitro
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