Relation Results

Summary

Name AMPK
Primary ID SIGNOR-C15
Links CPX-5633
Type complex
Formed by PRKAA2, PRKAB1, PRKAB2, PRKAG2, PRKAG3, PRKAA1, PRKAG1
Relations 204
Pathways Acute Myeloid Leukemia, IDH-TET in AML, AMPK Signaling, Autophagy, Citric acid cycle, Circadian clock, FAP: Glucocorticoids, FAP: GCR-cAMP crosstalk, FLT3-ITD signaling, Glycolysis and Gluconeogenesis, Leptin Signaling, Macropinocytosis , MTOR Signaling, Myofiber: IL6 and atrophy, NPM1_new, YAP/TAZ regulation
Inhibitors glucose; ATP

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Type: Score: Layout: SPV 
0.3010.20.2660.250.20.3210.550.5160.4220.20.2810.20.4240.2430.2440.310.2440.2570.4180.3570.3170.3020.20.350.2560.3110.20.2850.2610.470.20.20.2870.2520.20.20.2740.2660.3220.4040.3970.3980.20.2590.3790.3920.20.8230.2440.4840.20.4070.2950.20.2910.2860.2750.2920.5020.3980.20.2510.20.20.2580.4790.3970.5420.20.3780.3030.20.3230.20.20.20.20.2540.20.470.7370.4490.3180.410.3060.80.70.8660.8230.7960.20.80.5960.4640.8550.2660.7090.2880.7560.3880.3090.4110.3370.20.3550.70.2750.2750.2480.2840.590.8240.2740.80.410.20.80.384AMPKGLI1KPNA2NOS3HIPK2SLC12A1GAPDHMTORTSC2TP53MFFHDAC7CTBP1CRTC2PRPS2PRPS1HAT1PAK2MAPTTBC1D1EPM2AHDAC5RAF1CHKACRY1PDHA1HNF4APIKFYVETXNIPRBBP7ULK1VASPCCNYPPP1R3CCTHNR2C2BAIAP2CDC27CSNK1ESREBF1EEF2KFOXO3FOXOARMC10PPP1R12CPFKFB3PFKFB2ZNF692PRKAA1PLD1PPARGC1ASLC9A3MLXIPLKCNA5MCUKLC2RRN3ACSS2DNMT1RPTORCFTRFHATG9ASLC12A2AMOTL1NEDD4LGYS1GYS2ACACARB1LIPEYAP1TET2SCN5AYBX2GBF1SIRT7PAQR3ALDH2GbetaPRKAG2mTORC1WDR45ULK1/Atg13/Fip200ULK2glucoseGlycolysisPRKAB1PRMT6ATPSTK11TSCPRKAB2RAC1PRKAG3CRTC1PRKAG1PRKACAINSRTSC1FOXO4CAMK2BFOXO1StarvationHDAC4NAMPTIL6MAPK14CAMKK2PRKAA2CPS1ADPTMIGD2acadesineLEPR

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▶Modifications Tables

Relations
Regulator
Mechanism
target
score ℹ
+ AMPKdown-regulates quantity by destabilization img/direct_inhibition.png phosphorylation GLI1 0.301
Identifier Residue Sequence Organism Cell Line
SIGNOR-253542 Ser102 LQTVIRTsPSSLVAF
pmid sentence
Activation of AMPK reduces GLI1 protein levels and stability, thus blocking Sonic-hedgehog-induced transcriptional activity. AMPK phosphorylates GLI1 at serines 102 and 408 and threonine 1074. Mutation of these three sites into alanine prevents phosphorylation by AMPK. This leads to increased GLI1 protein stability, transcriptional activity, and oncogenic potency.
Identifier Residue Sequence Organism Cell Line
SIGNOR-253541 Ser408 GPLPRAPsISTVEPK
pmid sentence
Activation of AMPK reduces GLI1 protein levels and stability, thus blocking Sonic-hedgehog-induced transcriptional activity. AMPK phosphorylates GLI1 at serines 102 and 408 and threonine 1074. Mutation of these three sites into alanine prevents phosphorylation by AMPK. This leads to increased GLI1 protein stability, transcriptional activity, and oncogenic potency.
Identifier Residue Sequence Organism Cell Line
SIGNOR-253540 Ser408 GPLPRAPsISTVEPK
pmid sentence
Indeed we show that AMPK phosphorylates Gli1 at the unique residue Ser408, which is conserved only in primates but not in other species. Once phosphorylated, Gli1 is targeted for proteasomal degradation.
Identifier Residue Sequence Organism Cell Line
SIGNOR-253543 Thr1074 QRGSSGHtPPPSGPP
pmid sentence
Activation of AMPK reduces GLI1 protein levels and stability, thus blocking Sonic-hedgehog-induced transcriptional activity. AMPK phosphorylates GLI1 at serines 102 and 408 and threonine 1074. Mutation of these three sites into alanine prevents phosphorylation by AMPK. This leads to increased GLI1 protein stability, transcriptional activity, and oncogenic potency.
Publications: 4
+ AMPKup-regulates img/direct-activation.png phosphorylation KPNA2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-216449 Ser105 QAARKLLsREKQPPI Homo sapiens
pmid sentence
Ampk phosphorylated importin alpha1 on ser(105). Accordingly, expression of importin alpha1 proteins bearing k22r or s105a mutations failed to mediate the nuclear import of hur in intact cells. Our results point to importin alpha1 as a critical downstream target of ampk and key mediator of ampk-triggered hur nuclear import.
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates img/direct-activation.png phosphorylation NOS3 0.266
Identifier Residue Sequence Organism Cell Line
SIGNOR-216445 Ser1177 TSRIRTQsFSLQERQ Homo sapiens
pmid sentence
Recently many investigators have shown that protein phosphorylation of enos by several serine/threonine kinases is a critical control step for no production by endothelial cells. Phosphorylation by amp kinase, akt (or protein kinase b), or protein kinase a on serine 1179 (bovine) or serine 1177 (human) of enos leads to enhanced activity of the enzyme and, thus, augmented production of no.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216627 Ser1177 TSRIRTQsFSLQERQ Homo sapiens HeLa Cell
pmid sentence
The central finding of this report is that rosiglitazone rapidly stimulates no production and enos ser-1177 phosphorylation in an ampk-dependent manner
Publications: 2 Organism: Homo Sapiens
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation HIPK2 0.25
Identifier Residue Sequence Organism Cell Line
SIGNOR-275483 Ser121 LMRRSTVsLLDTYQK
pmid sentence
AMPKalpha2-mediated inhibition of WIP1 phosphorylation by HIPK2|Site-directed mutagenesis of Thr112 and Ser114 in the N terminus, and Thr1107 in the C terminus markedly reduced HIPK2 phosphorylation by AMPKalpha2 in vitro
Identifier Residue Sequence Organism Cell Line
SIGNOR-275484 Thr1116 AALGSTGtVAHLVAS
pmid sentence
AMPKalpha2-mediated inhibition of WIP1 phosphorylation by HIPK2|Site-directed mutagenesis of Thr112 and Ser114 in the N terminus, and Thr1107 in the C terminus markedly reduced HIPK2 phosphorylation by AMPKalpha2 in vitro
Identifier Residue Sequence Organism Cell Line
SIGNOR-275482 Thr119 HNLMRRStVSLLDTY
pmid sentence
AMPKalpha2-mediated inhibition of WIP1 phosphorylation by HIPK2|Site-directed mutagenesis of Thr112 and Ser114 in the N terminus, and Thr1107 in the C terminus markedly reduced HIPK2 phosphorylation by AMPKalpha2 in vitro
Publications: 3
+ AMPKup-regulates activity img/direct-activation.png phosphorylation SLC12A1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-263103 Ser122 YRNTGSIsGPKVNRP Mus musculus MMDD1 Cell
pmid sentence
In the present study, we demonstrate that the metabolic sensing kinase AMPK (AMP-activated protein kinase) phosphorylates NKCC2 on Ser126 in vitro. Activation of AMPK in the MMDD1 (mouse macula densa-derived 1) cell line resulted in an increase in Ser126 phosphorylation in situ, suggesting that AMPK may phosphorylate NKCC2 in vivo. The functional significance of Ser126 phosphorylation was examined by mutating the serine residue to an alanine residue resulting in a marked reduction in co-transporter activity when exogenously expressed in Xenopus laevis oocytes under isotonic conditions.
Publications: 1 Organism: Mus Musculus
+ AMPKup-regulates activity img/direct-activation.png phosphorylation GAPDH 0.321
Identifier Residue Sequence Organism Cell Line
SIGNOR-267578 Ser122 GAKRVIIsAPSADAP Mus musculus
pmid sentence
Under glucose starvation, but not amino acid starvation, cytoplasmic GAPDH is phosphorylated on Ser122 by activated AMPK. This causes GAPDH to redistribute into the nucleus. Inside the nucleus, GAPDH interacts directly with Sirt1, displacing Sirt1's repressor and causing Sirt1 to become activated. 
Publications: 1 Organism: Mus Musculus
Pathways:Glycolysis and Gluconeogenesis
+ AMPKup-regulates activity img/direct-activation.png phosphorylation MTOR 0.55
Identifier Residue Sequence Organism Cell Line
SIGNOR-262535 Ser1261 PMKKLHVsTINLQKA Mus musculus MEF Cell
pmid sentence
AMPK directly activates mTORC2 to promote cell survival during acute energetic stress. AMPK associates with and phosphorylates mTOR within mTORC2., these data indicate that AMPK phosphorylates mTOR on Ser1261 within mTORC2, an event that correlates with increased mTORC2 autophosphorylation and downstream signaling.
Publications: 1 Organism: Mus Musculus
Pathways:Acute Myeloid Leukemia, IDH-TET in AML, FLT3-ITD signaling, Leptin Signaling, MTOR Signaling
+ AMPKup-regulates img/direct-activation.png phosphorylation TSC2 0.516
Identifier Residue Sequence Organism Cell Line
SIGNOR-216438 Ser1387 QPLSKSSsSPELQTL Homo sapiens
pmid sentence
We have observed that ampk directly phosphorylates tsc2, and the ampk-dependent phosphorylation of tsc2 is critical for the coordination between cell growth and cellular energy levels. Phosphorylation of tsc2 by ampk is required for translation regulation and cell size control in response to energy deprivation.
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates img/direct-activation.png phosphorylation TP53 0.422
Identifier Residue Sequence Organism Cell Line
SIGNOR-216475 Ser15 PSVEPPLsQETFSDL Homo sapiens
pmid sentence
Ampk activation induces phosphorylation of p53 on serine 15, and this phosphorylation is required to initiate ampk-dependent cell-cycle arrest
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, IDH-TET in AML, FLT3-ITD signaling, Glycolysis and Gluconeogenesis, NPM1_new
+ AMPKup-regulates activity img/direct-activation.png phosphorylation MFF 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-245948 Ser155 GRLKRERsMSENAVR Homo sapiens U2-OS Cell
pmid sentence
A screen for substrates of AMPK identified mitochondrial fission factor (MFF), a mitochondrial outer-membrane receptor for DRP1, the cytoplasmic guanosine triphosphatase that catalyzes mitochondrial fission.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249655 Ser172 GQLVRNDsLWHRSDS Homo sapiens U2-OS Cell
pmid sentence
A screen for substrates of AMPK identified mitochondrial fission factor (MFF), a mitochondrial outer-membrane receptor for DRP1, the cytoplasmic guanosine triphosphatase that catalyzes mitochondrial fission.
Publications: 2 Organism: Homo Sapiens
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation HDAC7 0.281
Identifier Residue Sequence Organism Cell Line
SIGNOR-216554 Ser155 FPLRKTVsEPNLKLR Homo sapiens
pmid sentence
Another recently described set of transcriptional regulators targeted by ampk and its related family members across a range of eukaryotes are the class iia family of histone deacetylases (hdacs).
Identifier Residue Sequence Organism Cell Line
SIGNOR-216558 Ser358 WPLSRTRsEPLPPSA Homo sapiens
pmid sentence
Another recently described set of transcriptional regulators targeted by ampk and its related family members across a range of eukaryotes are the class iia family of histone deacetylases (hdacs).
Publications: 2 Organism: Homo Sapiens
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation CTBP1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-216604 Ser158 REGTRVQsVEQIREV Homo sapiens
pmid sentence
We found that an activated amp-activated protein kinase (ampk) phosphorylates ctbp1 on ser-158 upon metabolic stresses. Moreover, ampk-mediated phosphorylation of ctbp1 (s158) attenuates the repressive function of ctbp1
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation CRTC2 0.424
Identifier Residue Sequence Organism Cell Line
SIGNOR-216541 Ser170 PSALNRTsSDSALHT Homo sapiens
pmid sentence
Collectively, these findings suggest ampk suppresses glucose production through two transcriptional effects:reduced expression of creb targets via crtc inactivation and reduced expression of foxo target genes via class iia hdac inactivation
Identifier Residue Sequence Organism Cell Line
SIGNOR-216576 Ser171 SALNRTSsDSALHTS Mus musculus Hepatocyte
pmid sentence
Phosphorylation on the ser171 residue of crtc2 by ampk and ampk-related kinases, including the salt-inducible kinases (siks), is critical for determining the activity, cellular localization, and degradation of crtc2
Publications: 2 Organism: Homo Sapiens, Mus Musculus
Pathways:Glycolysis and Gluconeogenesis
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation PRPS2 0.243
Identifier Residue Sequence Organism Cell Line
SIGNOR-265732 Ser180 GGAKRVTsIADRLNV Homo sapiens LK-87 Cell
pmid sentence
We demonstrate here that glucose deprivation or hypoxia results in the AMPK-mediated phosphorylation of phosphoribosyl pyrophosphate synthetase 1 (PRPS1) S180 and PRPS2 S183, leading to conversion of PRPS hexamers to monomers and thereby inhibiting PRPS1/2 activity, nucleotide synthesis, and nicotinamide adenine dinucleotide (NAD) production.
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation PRPS1 0.244
Identifier Residue Sequence Organism Cell Line
SIGNOR-265730 Ser180 GGAKRVTsIADRLNV Homo sapiens LK-87 Cell
pmid sentence
We demonstrate here that glucose deprivation or hypoxia results in the AMPK-mediated phosphorylation of phosphoribosyl pyrophosphate synthetase 1 (PRPS1) S180 and PRPS2 S183, leading to conversion of PRPS hexamers to monomers and thereby inhibiting PRPS1/2 activity, nucleotide synthesis, and nicotinamide adenine dinucleotide (NAD) production.
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation HAT1 0.31
Identifier Residue Sequence Organism Cell Line
SIGNOR-264787 Ser190 MWFIETAsFIDVDDE in vitro
pmid sentence
Together, these results indicate that AMPK phosphorylated DNMT1-Ser730, RBBP7-Ser314, and HAT1-Ser190|AMPK increased HAT1 activity through phosphorylation of HAT1-Ser190 and RBBP7-Ser314
Publications: 1 Organism: In Vitro
+ AMPK img/unknown.png phosphorylation PAK2 0.244
Identifier Residue Sequence Organism Cell Line
SIGNOR-216612 Ser20 APPVRMSsTIFSTGG Homo sapiens
pmid sentence
Together, these results indicate that ampk phosphorylates endogenous ppp1r12c at s452 and pak2 at s20 in human cells.
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation MAPT 0.257
Identifier Residue Sequence Organism Cell Line
SIGNOR-273926 Ser214 GGKERPGsKEEVDED in vitro
pmid sentence
AMPK phosphorylation inhibits tau binding of microtubules. In order to study further the phosphorylation of tau by AMPK, we compared phosphorylation of tau by MARK4 or AMPK using a panel of phospho-tau antibodies (Figure 2A). Five phosphorylation sites common to both kinases were identified (Thr231, Ser262, Ser356, Ser396 and Ser422). In addition, AMPK, but not MARK4, was capable of phosphorylating Ser214 (Figure 2A).
Identifier Residue Sequence Organism Cell Line
SIGNOR-273930 Ser396 DDKKAKTsTRSSAKT in vitro
pmid sentence
AMPK phosphorylation inhibits tau binding of microtubules. In order to study further the phosphorylation of tau by AMPK, we compared phosphorylation of tau by MARK4 or AMPK using a panel of phospho-tau antibodies (Figure 2A). Five phosphorylation sites common to both kinases were identified (Thr231, Ser262, Ser356, Ser396 and Ser422). In addition, AMPK, but not MARK4, was capable of phosphorylating Ser214 (Figure 2A).
Identifier Residue Sequence Organism Cell Line
SIGNOR-273931 Ser420 KHPTPGSsDPLIQPS in vitro
pmid sentence
AMPK phosphorylation inhibits tau binding of microtubules. In order to study further the phosphorylation of tau by AMPK, we compared phosphorylation of tau by MARK4 or AMPK using a panel of phospho-tau antibodies (Figure 2A). Five phosphorylation sites common to both kinases were identified (Thr231, Ser262, Ser356, Ser396 and Ser422). In addition, AMPK, but not MARK4, was capable of phosphorylating Ser214 (Figure 2A).
Publications: 3 Organism: In Vitro
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation TBC1D1 0.418
Identifier Residue Sequence Organism Cell Line
SIGNOR-216631 Ser237 RPMRKSFsQPGLRSL Homo sapiens
pmid sentence
In rat l6 myotubes, endogenous tbc1d1 is strongly phosphorylated on ser237 and binds to 14-3-3s in response to the ampk activators aicar
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation EPM2A 0.357
Identifier Residue Sequence Organism Cell Line
SIGNOR-277830 Ser25 PELLVVGsRPELGRW Homo sapiens HEK-293 Cell
pmid sentence
We demonstrate that laforin is a phosphoprotein, as indicated by two-dimensional electrophoresis, and we identify Ser(25) as the residue involved in this modification. We also show that Ser(25) is phosphorylated both in vitro and in vivo by AMPK. Lastly, we demonstrate that this residue plays a critical role for both the phosphatase activity and the ability of laforin to interact with itself and with previously established binding partners.
Identifier Residue Sequence Organism Cell Line
SIGNOR-276338 Ser25 PELLVVGsRPELGRW Homo sapiens HEK-293 Cell
pmid sentence
In the present study, we demonstrate that laforin is a phosphoprotein, as indicated by two-dimensional electrophoresis, and we identify Ser(25) as the residue involved in this modification. We also show that Ser(25) is phosphorylated both in vitro and in vivo by AMPK. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-271730 in vitro
pmid sentence
Here, we provide evidence indicating that the formation of the laforin–malin complex is positively regulated by AMPK. We show that laforin, but not malin, can interact physically with the catalytic subunit of AMPK and that purified AMPK phosphorylates GST::laforin in vitro.
Publications: 3 Organism: Homo Sapiens, In Vitro
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation HDAC5 0.317
Identifier Residue Sequence Organism Cell Line
SIGNOR-216596 Ser259 FPLRKTAsEPNLKVR Homo sapiens
pmid sentence
Another recently described set of transcriptional regulators targeted by ampk and its related family members across a range of eukaryotes are the class iia family of histone deacetylases (hdacs)
Identifier Residue Sequence Organism Cell Line
SIGNOR-216550 Ser498 RPLSRTQsSPLPQSP Homo sapiens
pmid sentence
Another recently described set of transcriptional regulators targeted by ampk and its related family members across a range of eukaryotes are the class iia family of histone deacetylases (hdacs)
Publications: 2 Organism: Homo Sapiens
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation RAF1 0.302
Identifier Residue Sequence Organism Cell Line
SIGNOR-216523 Ser259 SQRQRSTsTPNVHMV Homo sapiens
pmid sentence
Mutation of serine 259 increased the basal raf-1 activity and rendered it largely resistant to inhibition by pka.
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation MAPT (isoform 5) 0.257
Identifier Residue Sequence Organism Cell Line
SIGNOR-273928 Ser262 NVKSKIGsTENLKHQ in vitro
pmid sentence
AMPK phosphorylation inhibits tau binding of microtubules. In order to study further the phosphorylation of tau by AMPK, we compared phosphorylation of tau by MARK4 or AMPK using a panel of phospho-tau antibodies (Figure 2A). Five phosphorylation sites common to both kinases were identified (Thr231, Ser262, Ser356, Ser396 and Ser422). In addition, AMPK, but not MARK4, was capable of phosphorylating Ser214 (Figure 2A).
Identifier Residue Sequence Organism Cell Line
SIGNOR-273927 Thr231 KKVAVVRtPPKSPSS in vitro
pmid sentence
AMPK phosphorylation inhibits tau binding of microtubules. In order to study further the phosphorylation of tau by AMPK, we compared phosphorylation of tau by MARK4 or AMPK using a panel of phospho-tau antibodies (Figure 2A). Five phosphorylation sites common to both kinases were identified (Thr231, Ser262, Ser356, Ser396 and Ser422). In addition, AMPK, but not MARK4, was capable of phosphorylating Ser214 (Figure 2A).
Publications: 2 Organism: In Vitro
+ AMPKup-regulates activity img/direct-activation.png phosphorylation CHKA 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-267647 Ser279 KKLHKLLsYNLPLEL Homo sapiens Glioblastoma Cell
pmid sentence
Glucose deprivation induces the binding of choline kinase α2 (CHKα2) to lipid droplets, followed by a continuous PTMs to promote lipolysis of lipid droplets, which are in turn mediated by AMPK-dependent CHKα2 Serine 279 phosphorylation and KAT5-dependent CHKα2 Lysine 247 acetylation.
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates quantity by destabilization img/direct_inhibition.png phosphorylation CRY1 0.35
Identifier Residue Sequence Organism Cell Line
SIGNOR-268047 Ser280 YKKVKKNsSPPLSLY Homo sapiens
pmid sentence
We demonstrated that the nutrient-responsive adenosine monophosphate-activated protein kinase (AMPK) phosphorylates and destabilizes the clock component cryptochrome 1 (CRY1). In mouse livers, AMPK activity and nuclear localization were rhythmic and inversely correlated with CRY1 nuclear protein abundance. Stimulation of AMPK destabilized cryptochromes and altered circadian rhythms, and mice in which the AMPK pathway was genetically disrupted showed alterations in peripheral clocks. Phosphorylation of S71 or S280 by AMPK destabilizes CRY1
Identifier Residue Sequence Organism Cell Line
SIGNOR-268046 Ser71 ANLRKLNsRLFVIRG Homo sapiens
pmid sentence
We demonstrated that the nutrient-responsive adenosine monophosphate-activated protein kinase (AMPK) phosphorylates and destabilizes the clock component cryptochrome 1 (CRY1). In mouse livers, AMPK activity and nuclear localization were rhythmic and inversely correlated with CRY1 nuclear protein abundance. Stimulation of AMPK destabilized cryptochromes and altered circadian rhythms, and mice in which the AMPK pathway was genetically disrupted showed alterations in peripheral clocks. Phosphorylation of S71 or S280 by AMPK destabilizes CRY1
Publications: 2 Organism: Homo Sapiens
Pathways:Circadian clock
+ AMPKup-regulates activity img/direct-activation.png phosphorylation PDHA1 0.256
Identifier Residue Sequence Organism Cell Line
SIGNOR-276835 Ser295 RYHGHSMsDPGVSYR in vitro
pmid sentence
In vitro kinase assay revealed that PDHA could be readily phosphorylated by active AMPK complex in a dose-dependent manner (Figure 6C). 
Identifier Residue Sequence Organism Cell Line
SIGNOR-277895 Ser295 RYHGHSMsDPGVSYR in vitro
pmid sentence
AMPK localizes in the mitochondrial matrix and phosphorylates the catalytic alpha subunit of PDHc (PDHA) on two residues S295 and S314, which activates the enzymatic activity of PDHc and alleviates an inhibitory phosphorylation by PDHKs, respectively. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-276836 Ser314 IQEVRSKsDPIMLLK in vitro
pmid sentence
In vitro kinase assay revealed that PDHA could be readily phosphorylated by active AMPK complex in a dose-dependent manner (Figure 6C). 
Identifier Residue Sequence Organism Cell Line
SIGNOR-277894 Ser314 IQEVRSKsDPIMLLK in vitro
pmid sentence
AMPK localizes in the mitochondrial matrix and phosphorylates the catalytic alpha subunit of PDHc (PDHA) on two residues S295 and S314, which activates the enzymatic activity of PDHc and alleviates an inhibitory phosphorylation by PDHKs, respectively. 
Publications: 4 Organism: In Vitro
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation HNF4A 0.311
Identifier Residue Sequence Organism Cell Line
SIGNOR-216511 Ser303 DPDAKGLsDPGKIKR Homo sapiens
pmid sentence
Here we demonstrate that ampk directly phosphorylates hnf4 and represses its transcriptional activity. Ampk-mediated phosphorylation of hnf4 on serine 304 had a 2-fold effect
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation HNF4A 0.311
Identifier Residue Sequence Organism Cell Line
SIGNOR-263106 Ser303 DPDAKGLsDPGKIKR in vitro
pmid sentence
Here we demonstrate that AMPK directly phosphorylates HNF4alpha and represses its transcriptional activity. AMPK-mediated phosphorylation of HNF4alpha on serine 304 had a 2-fold effect, reducing the ability of the transcription factor to form homodimers and bind DNA and increasing its degradation rate in vivo.
Publications: 1 Organism: In Vitro
+ AMPKup-regulates activity img/direct-activation.png phosphorylation PIKFYVE 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-263031 Ser307 PARNRSAsITNLSLD in vitro
pmid sentence
AMPK phosphorylated PIKfyve at Ser307 both in vitro and in intact cells. We propose that PIKfyve activity is required for the stimulation of skeletal muscle glucose uptake by contraction/AMPK activation. PIKfyve is a new AMPK substrate whose phosphorylation at Ser307 could promote PIKfyve translocation to endosomes for PtdIns(3,5)P2 synthesis to facilitate GLUT4 (glucose transporter 4) translocation.
Publications: 1 Organism: In Vitro
+ AMPKdown-regulates quantity by destabilization img/direct_inhibition.png phosphorylation TXNIP 0.285
Identifier Residue Sequence Organism Cell Line
SIGNOR-276489 Ser308 GLSSRTSsMASRTSS Rattus norvegicus Hepatocyte
pmid sentence
AMPK phosphorylation of TXNIP on S308 accelerates its degradation
Publications: 1 Organism: Rattus Norvegicus
+ AMPKup-regulates activity img/direct-activation.png phosphorylation RBBP7 0.261
Identifier Residue Sequence Organism Cell Line
SIGNOR-264789 Ser314 LKLHTFEsHKDEIFQ in vitro
pmid sentence
AMPK increased HAT1 activity through phosphorylation of HAT1-Ser190 and RBBP7-Ser314| interaction between RBBP7 and HAT1 is required for acetyltransferase activity
Publications: 1 Organism: In Vitro
+ AMPKup-regulates img/direct-activation.png phosphorylation ULK1 0.47
Identifier Residue Sequence Organism Cell Line
SIGNOR-216453 Ser317 SHLASPPsLGEMQQL Homo sapiens
pmid sentence
In a screen for conserved substrates of ampk, we identified ulk1 and ulk2, mammalian orthologs of the yeast protein kinase atg1, which is required for autophagy.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216491 Ser317 SHLASPPsLGEMQQL Homo sapiens
pmid sentence
In a screen for conserved substrates of ampk, we identified ulk1 and ulk2, mammalian orthologs of the yeast protein kinase atg1, which is required for autophagy.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216495 Ser556 GLGCRLHsAPNLSDL Homo sapiens
pmid sentence
In a screen for conserved substrates of ampk, we identified ulk1 and ulk2, mammalian orthologs of the yeast protein kinase atg1, which is required for autophagy.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216457 Ser556 GLGCRLHsAPNLSDL Homo sapiens
pmid sentence
In a screen for conserved substrates of ampk, we identified ulk1 and ulk2, mammalian orthologs of the yeast protein kinase atg1, which is required for autophagy.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216461 Ser638 FDFPKTPsSQNLLAL Homo sapiens
pmid sentence
In a screen for conserved substrates of ampk, we identified ulk1 and ulk2, mammalian orthologs of the yeast protein kinase atg1, which is required for autophagy.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216499 Ser638 FDFPKTPsSQNLLAL Homo sapiens
pmid sentence
In a screen for conserved substrates of ampk, we identified ulk1 and ulk2, mammalian orthologs of the yeast protein kinase atg1, which is required for autophagy.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216464 Homo sapiens
pmid sentence
Ampk and ulk1 interact and that the latter is phosphorylated by ampk. This phosphorylation leads to the direct activation of ulk1 by ampk bypassing mtor-inhibition.
Publications: 7 Organism: Homo Sapiens
Pathways:MTOR Signaling
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation VASP 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-216568 Ser322 TTLPRMKsSSSVTTS Homo sapiens
pmid sentence
Here we show that phosphorylation of vasp by ampk occurs at a novel site, serine 322, and that phosphorylation at this site alters actin filament binding. We also show that inhibition of ampk activity results in the accumulation of vasp at cell-cell adhesions and a concomitant increase in cell-cell adhesion.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216515 Thr278 LARRRKAtQVGEKTP Homo sapiens
pmid sentence
Pharmacological ampk inhibitors and activators and ampk mutants revealed that the kinase specifically targets residue thr-278 but not ser-157 or ser-239. Quantitative fluorescence-activated cell sorter analysis and serum response factor transcriptional reporter assays, which quantify the cellular f-/g-actin equilibrium, indicated that ampk-mediated vasp phosphorylation impaired actin stress fiber formation and altered cell morphology.
Publications: 2 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation CCNY 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-273001 Ser326 SARKRSAsADNLTLP Homo sapiens
pmid sentence
Our in vitro and cellular analyses supported the mass spectrometry data that implicated serine 326 (S326) as the phospho-acceptor site on CCNY by AMPK. |Mechanistically the S326 phosphorylation by AMPK promotes the interaction of CCNY with CDK16, which in turn autophosphorylates S336, which serves as a marker for active CCNY-CDK16
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates quantity by destabilization img/direct_inhibition.png phosphorylation PPP1R3C 0.287
Identifier Residue Sequence Organism Cell Line
SIGNOR-273739 Ser33 MRLCLAHsPPVKSFL Cricetulus griseus CHO Cell
pmid sentence
AMPK induces the phosphorylation of residues Ser-8 and Ser-268 in R5/PTG. We now demonstrate that phosphorylation of R5/PTG at Ser-8 by AMPK accelerates its laforin/malin-dependent ubiquitination and subsequent proteasomal degradation, which results in a decrease of its glycogenic activity. Thus, our results define a novel role of AMPK in glycogen homeostasis.
Identifier Residue Sequence Organism Cell Line
SIGNOR-276237 Ser33 MRLCLAHsPPVKSFL Homo sapiens HEK-293 Cell
pmid sentence
We have recently described that the activity of R5/PTG is down-regulated by the laforin-malin complex, composed of a dual specificity phosphatase (laforin) and an E3-ubiquitin ligase (malin). We now demonstrate that phosphorylation of R5/PTG at Ser-8 by AMPK accelerates its laforin/malin-dependent ubiquitination and subsequent proteasomal degradation, which results in a decrease of its glycogenic activity. 
Publications: 2 Organism: Cricetulus Griseus, Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation CTH 0.252
Identifier Residue Sequence Organism Cell Line
SIGNOR-277808 Ser346 ESLGGFEsLAELPAI Homo sapiens HEK-293 Cell
pmid sentence
 Here we define an important mechanism of increased persulfide and polysulfide production involving cystathionine gamma lyase (CSE) phosphorylation at serine 346 and threonine 355 in a substrate specific manner, under acute hypoxic conditions. Hypoxic phosphorylation of CSE occurs in an AMP kinase dependent manner increasing enzyme activity involving unique inter- and intramolecular interactions within the tetramer. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-277794 Ser346 ESLGGFEsLAELPAI Homo sapiens HEK-293 Cell
pmid sentence
 Here we define an important mechanism of increased persulfide and polysulfide production involving cystathionine gamma lyase (CSE) phosphorylation at serine 346 and threonine 355 in a substrate specific manner, under acute hypoxic conditions. Hypoxic phosphorylation of CSE occurs in an AMP kinase dependent manner increasing enzyme activity involving unique inter- and intramolecular interactions within the tetramer. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-277793 Thr355 AELPAIMtHASVLKN Homo sapiens HEK-293 Cell
pmid sentence
 Here we define an important mechanism of increased persulfide and polysulfide production involving cystathionine gamma lyase (CSE) phosphorylation at serine 346 and threonine 355 in a substrate specific manner, under acute hypoxic conditions. Hypoxic phosphorylation of CSE occurs in an AMP kinase dependent manner increasing enzyme activity involving unique inter- and intramolecular interactions within the tetramer. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-277807 Thr355 AELPAIMtHASVLKN Homo sapiens HEK-293 Cell
pmid sentence
 Here we define an important mechanism of increased persulfide and polysulfide production involving cystathionine gamma lyase (CSE) phosphorylation at serine 346 and threonine 355 in a substrate specific manner, under acute hypoxic conditions. Hypoxic phosphorylation of CSE occurs in an AMP kinase dependent manner increasing enzyme activity involving unique inter- and intramolecular interactions within the tetramer. 
Publications: 4 Organism: Homo Sapiens
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation NR2C2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-216537 Ser351 HVISRDQsTPIIEVE Homo sapiens
pmid sentence
Tr4 transactivation is inhibited via phosphorylation bymetformin-induced amp-activated protein kinase (ampk) at the amino acid serine 351, which results in the suppression of scd1 gene expression
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation MAPT (isoform 4) 0.257
Identifier Residue Sequence Organism Cell Line
SIGNOR-273929 Ser352 PRHLSNVsSTGSIDM in vitro
pmid sentence
AMPK phosphorylation inhibits tau binding of microtubules. In order to study further the phosphorylation of tau by AMPK, we compared phosphorylation of tau by MARK4 or AMPK using a panel of phospho-tau antibodies (Figure 2A). Five phosphorylation sites common to both kinases were identified (Thr231, Ser262, Ser356, Ser396 and Ser422). In addition, AMPK, but not MARK4, was capable of phosphorylating Ser214 (Figure 2A).
Publications: 1 Organism: In Vitro
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation BAIAP2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-216608 Ser366 KTLPRSSsMAAGLER Homo sapiens
pmid sentence
Using this approach for ppp1r12c, baiap2, and cdc27, we found that mutation of a single serine to alanine (s452, s366, and s379 respectively) resulted in almost a complete loss of ampk phosphorylation in these proteins. Termination of irsp53 function is suggested to occur following cdc42 dissociation, kinase phosphorylation of t340 and t360, and subsequent 14-3-3 binding, which competes for sh3 partners, thus allowing filopodial retraction
Identifier Residue Sequence Organism Cell Line
SIGNOR-216572 Ser366 KTLPRSSsMAAGLER Homo sapiens
pmid sentence
Using this approach for ppp1r12c, baiap2, and cdc27, we found that mutation of a single serine to alanine (s452, s366, and s379 respectively) resulted in almost a complete loss of ampk phosphorylation in these proteins. Termination of irsp53 function is suggested to occur following cdc42 dissociation, kinase phosphorylation of t340 and t360, and subsequent 14-3-3 binding, which competes for sh3 partners, thus allowing filopodial retraction
Publications: 2 Organism: Homo Sapiens
+ AMPK img/unknown.png phosphorylation CDC27 0.274
Identifier Residue Sequence Organism Cell Line
SIGNOR-216434 Ser379 NALPRRSsRLFTSDS Homo sapiens
pmid sentence
Using this approach for ppp1r12c, baiap2, and cdc27, we found that mutation of a single serine to alanine (s452, s366, and s379, respectively) resulted in an almost complete loss of ampk phosphorylation in these proteins
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation CSNK1E 0.266
Identifier Residue Sequence Organism Cell Line
SIGNOR-276064 Ser389 RGAPANVsSSDLTGR in vitro
pmid sentence
AMPK enhances mPer2 degradation and CKIɛ activity by phosphorylating Ser-389 of CKIɛ. One of the regulators of the period length is casein kinase Iepsilon (CKIepsilon), which by phosphorylating and inducing the degradation of the circadian clock component, mPer2, shortens the period length. AMPK phosphorylates Ser-389 of CKIepsilon, resulting in increased CKIepsilon activity and degradation of mPer2. 
Publications: 1 Organism: In Vitro
Pathways:Circadian clock
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation SREBF1 0.322
Identifier Residue Sequence Organism Cell Line
SIGNOR-216533 Ser396 TAVHKSKsLKDLVSA Homo sapiens
pmid sentence
Here we demonstrate that ampk interacts with and directly phosphorylates sterol regulatory element binding proteins (srebp-1c and -2). Ser372
Identifier Residue Sequence Organism Cell Line
SIGNOR-216564 Homo sapiens
pmid sentence
Ampk was recently found to phosphorylate a conserved serine near the cleavage site within srebp1, suppressing its activation
Publications: 2 Organism: Homo Sapiens
Pathways:Circadian clock, MTOR Signaling
+ AMPKup-regulates img/direct-activation.png phosphorylation EEF2K 0.404
Identifier Residue Sequence Organism Cell Line
SIGNOR-216503 Ser398 DSLPSSPsSATPHSQ Homo sapiens
pmid sentence
In response to genotoxic stress, eef2k was activated by ampk (adenosine monophosphate-activated protein kinase)-mediated phosphorylation on serine 398. Activated eef2k phosphorylated eef2 and induced a temporary ribosomal slowdown at the stage of elongation
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation FOXO3 0.397
Identifier Residue Sequence Organism Cell Line
SIGNOR-274095 Ser399 DNITLPPsQPSPTGG in vitro
pmid sentence
Here, we find that AMPK directly regulates mammalian FOXO3, a member of the FOXO family of Forkhead transcription factors known to promote resistance to oxidative stress, tumor suppression, and longevity. We show that AMPK phosphorylates human FOXO3 at six previously unidentified regulatory sites.Phosphorylation by AMPK leads to the activation of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.Taken together, these results indicate that AMPK phosphorylates at least six residues of FOXO3 in vitro (Thr179, Ser399, Ser413, Ser555, Ser588, and Ser626).
Identifier Residue Sequence Organism Cell Line
SIGNOR-249668 Ser399 DNITLPPsQPSPTGG Homo sapiens HEK-293 Cell
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-255756 Ser413 GLMQRSSsFPYTTKG Mus musculus
pmid sentence
When AMPK is stimulated, pre-existing FOXO3 becomes reverted toward an active form.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249678 Ser413 GLMQRSSsFPYTTKG Homo sapiens
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-274098 Ser413 GLMQRSSsFPYTTKG in vitro
pmid sentence
Here, we find that AMPK directly regulates mammalian FOXO3, a member of the FOXO family of Forkhead transcription factors known to promote resistance to oxidative stress, tumor suppression, and longevity. We show that AMPK phosphorylates human FOXO3 at six previously unidentified regulatory sites.Phosphorylation by AMPK leads to the activation of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.Taken together, these results indicate that AMPK phosphorylates at least six residues of FOXO3 in vitro (Thr179, Ser399, Ser413, Ser555, Ser588, and Ser626).
Identifier Residue Sequence Organism Cell Line
SIGNOR-274093 Ser555 RALSNSVsNMGLSES in vitro
pmid sentence
Here, we find that AMPK directly regulates mammalian FOXO3, a member of the FOXO family of Forkhead transcription factors known to promote resistance to oxidative stress, tumor suppression, and longevity. We show that AMPK phosphorylates human FOXO3 at six previously unidentified regulatory sites.Phosphorylation by AMPK leads to the activation of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.Taken together, these results indicate that AMPK phosphorylates at least six residues of FOXO3 in vitro (Thr179, Ser399, Ser413, Ser555, Ser588, and Ser626).
Identifier Residue Sequence Organism Cell Line
SIGNOR-249682 Ser555 RALSNSVsNMGLSES Homo sapiens
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-249685 Ser588 QTLSDSLsGSSLYST Homo sapiens
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-274096 Ser588 QTLSDSLsGSSLYST in vitro
pmid sentence
Here, we find that AMPK directly regulates mammalian FOXO3, a member of the FOXO family of Forkhead transcription factors known to promote resistance to oxidative stress, tumor suppression, and longevity. We show that AMPK phosphorylates human FOXO3 at six previously unidentified regulatory sites.Phosphorylation by AMPK leads to the activation of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.Taken together, these results indicate that AMPK phosphorylates at least six residues of FOXO3 in vitro (Thr179, Ser399, Ser413, Ser555, Ser588, and Ser626).
Identifier Residue Sequence Organism Cell Line
SIGNOR-249688 Ser626 SLECDMEsIIRSELM Homo sapiens
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-274097 Ser626 SLECDMEsIIRSELM in vitro
pmid sentence
Here, we find that AMPK directly regulates mammalian FOXO3, a member of the FOXO family of Forkhead transcription factors known to promote resistance to oxidative stress, tumor suppression, and longevity. We show that AMPK phosphorylates human FOXO3 at six previously unidentified regulatory sites.Phosphorylation by AMPK leads to the activation of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.Taken together, these results indicate that AMPK phosphorylates at least six residues of FOXO3 in vitro (Thr179, Ser399, Ser413, Ser555, Ser588, and Ser626).
Identifier Residue Sequence Organism Cell Line
SIGNOR-274094 Thr179 SSPDKRLtLSQIYEW in vitro
pmid sentence
Here, we find that AMPK directly regulates mammalian FOXO3, a member of the FOXO family of Forkhead transcription factors known to promote resistance to oxidative stress, tumor suppression, and longevity. We show that AMPK phosphorylates human FOXO3 at six previously unidentified regulatory sites.Phosphorylation by AMPK leads to the activation of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.Taken together, these results indicate that AMPK phosphorylates at least six residues of FOXO3 in vitro (Thr179, Ser399, Ser413, Ser555, Ser588, and Ser626).
Identifier Residue Sequence Organism Cell Line
SIGNOR-238813 Thr179 SSPDKRLtLSQIYEW Homo sapiens
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216481 Homo sapiens
pmid sentence
We have recently found that AMPK phosphorylates human FOXO3 in mammalian cells at novel regulatory sites that are distinct from the AKT sites
Publications: 14 Organism: In Vitro, Homo Sapiens, Mus Musculus
Pathways:AMPK Signaling
+ AMPKup-regulates activity img/direct-activation.png phosphorylation FOXO 0.398
Identifier Residue Sequence Organism Cell Line
SIGNOR-252880 Ser399 DNITLPPsQPSPTGG Homo sapiens HEK-293 Cell
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252881 Ser413 GLMQRSSsFPYTTKG Homo sapiens HEK-293 Cell
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252882 Ser555 RALSNSVsNMGLSES Homo sapiens HEK-293 Cell
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252883 Ser588 QTLSDSLsGSSLYST Homo sapiens HEK-293 Cell
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252884 Ser626 SLECDMEsIIRSELM Homo sapiens HEK-293 Cell
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252885 Thr179 SSPDKRLtLSQIYEW Homo sapiens HEK-293 Cell
pmid sentence
Phosphorylation by AMPK leads to the activtion of FOXO3 transcriptional activity without affecting FOXO3 subcellular localization.
Identifier Residue Sequence Organism Cell Line
SIGNOR-252889 Homo sapiens
pmid sentence
The energy sensor amp-activated protein kinase (ampk) has been shown to directly phosphorylate foxo factors at six regulatory sites that are distinct from the akt.
Publications: 7 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, IDH-TET in AML, FLT3-ITD signaling, NPM1_new
+ AMPKup-regulates activity img/direct-activation.png phosphorylation ARMC10 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-277792 Ser45 LGIRSSKsAGALEEG Homo sapiens HEK-293A Cell
pmid sentence
Further analysis using an AMPK consensus phosphorylation motif indicated that 32 of these sites are likely direct AMPK phosphorylation sites. We validated one uncharacterized protein, ARMC10, and demonstrated that the S45 site of ARMC10 can be phosphorylated by AMPK both in vitro and in vivo. Function assay of ARMC10 and ARMC10 phosphorylation at S45.
Identifier Residue Sequence Organism Cell Line
SIGNOR-277806 Ser45 LGIRSSKsAGALEEG Homo sapiens HEK-293A Cell
pmid sentence
Further analysis using an AMPK consensus phosphorylation motif indicated that 32 of these sites are likely direct AMPK phosphorylation sites. We validated one uncharacterized protein, ARMC10, and demonstrated that the S45 site of ARMC10 can be phosphorylated by AMPK both in vitro and in vivo. Function assay of ARMC10 and ARMC10 phosphorylation at S45.
Identifier Residue Sequence Organism Cell Line
SIGNOR-266413 Ser45 LGIRSSKsAGALEEG Homo sapiens
pmid sentence
We validated one uncharacterized protein, ARMC10, and demonstrated that the S45 site of ARMC10 can be phosphorylated by AMPK both in vitro and in vivo. Moreover, ARMC10 overexpression was sufficient to promote mitochondrial fission, whereas ARMC10 knockout prevented AMPK-mediated mitochondrial fission. These results demonstrate that ARMC10 is an effector of AMPK that participates in dynamic regulation of mitochondrial fission and fusion.
Publications: 3 Organism: Homo Sapiens
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation PPP1R12C 0.259
Identifier Residue Sequence Organism Cell Line
SIGNOR-216600 Ser452 AGLQRSAsSSWLEGT Homo sapiens
pmid sentence
Ampk-induced phosphorylation is necessary for ppp1r12c interaction with 14-3-3 and phosphorylation of myosin regulatory light chain. Both ampk activity and ppp1r12c phosphorylation are increased in mitotic cells and are important for mitosis completion. The interaction between ppp1r12c and 14-3-3_ may inactivate the ppp1r12c-containing phosphatase complex in vivo.
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates img/direct-activation.png phosphorylation PFKFB3 0.379
Identifier Residue Sequence Organism Cell Line
SIGNOR-216639 Ser461 NPLMRRNsVTPLASP Homo sapiens Monocyte
pmid sentence
Ipfk-2 was phosphorylated on the homologous serine (ser-461) and activated by ampk in vitro.
Publications: 1 Organism: Homo Sapiens
Tissue: Kidney
Pathways:Glycolysis and Gluconeogenesis
+ AMPKup-regulates activity img/direct-activation.png phosphorylation PFKFB2 0.392
Identifier Residue Sequence Organism Cell Line
SIGNOR-260011 Ser466 PVRMRRNsFTPLSSSN Rattus norvegicus
pmid sentence
AMPK phosphorylates and activates heart PFK-2 in vitro and in intact cells. AMPK-mediated PFK-2 activation is likely to be involved in the stimulation of heart glycolysis during ischaemia.
Identifier Residue Sequence Organism Cell Line
SIGNOR-209947 Ser466 PVRMRRNsFTPLSSS Homo sapiens
pmid sentence
The decreased glycogen synthesis rates upon acute AMPK activation are generally coupled to an increase in the glycolytic flux, thanks to the activation of 6-phosphofructo-2-kinase (PFK-2) through direct phosphorylation on Ser466 [35]. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate, a potent stimulator of glycolysis. Therefore, activation of AMPK rapidly mobilizes glucose into ATP-generating processes.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216623 Ser466 PVRMRRNsFTPLSSS Homo sapiens
pmid sentence
Heart 6-phosphofructo-2-kinase activation by insulin results from ser-466 and ser-483 phosphorylation and requires 3-phosphoinositide-dependent kinase-1, but not protein kinase b.
Publications: 3 Organism: Rattus Norvegicus, Homo Sapiens
Tissue: Heart
Pathways:AMPK Signaling
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation ZNF692 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-216519 Ser470 VAAHRSKsHPALLLA Homo sapiens
pmid sentence
Arebp is phosphorylated at ser(470) by ampk. Phosphorylation reduces the dna-binding activity of arebp.
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation PRKAA1 0.823
Identifier Residue Sequence Organism Cell Line
SIGNOR-256113 Ser486 DDEITEAKsGTATPQRS in vitro
pmid sentence
We show that AMPK α-Ser485/491 can be a site for autophosphorylation, which may play a role in limiting AMPK activation in response to energy depletion or other regulators
Publications: 1 Organism: In Vitro
+ AMPKup-regulates img/direct-activation.png phosphorylation PLD1 0.244
Identifier Residue Sequence Organism Cell Line
SIGNOR-216643 Ser505 GSVKRVTsGPSLGSL Homo sapiens
pmid sentence
Ampk-wild type (wt) stimulates pld activity, while ampk-dominant negative (dn) inhibits it. Ampk regulates pld1 activity through phosphorylation of the ser-505 and this phosphorylation is increased by the presence of amp.
Publications: 1 Organism: Homo Sapiens
Tissue: Muscle, Skeletal Muscle
+ AMPKup-regulates img/direct-activation.png phosphorylation PPARGC1A 0.484
Identifier Residue Sequence Organism Cell Line
SIGNOR-216647 Ser539 SLFNVSPsCSSFNSP Homo sapiens
pmid sentence
Ampk phosphorylates pgc-1alpha directly both in vitro and in cells. These direct phosphorylations of the pgc-1alpha protein at threonine-177 and serine-538.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216651 Thr178 NHNHRIRtNPAIVKT Homo sapiens
pmid sentence
Ampk phosphorylates pgc-1alpha directly both in vitro and in cells. These direct phosphorylations of the pgc-1alpha protein at threonine-177 and serine-538.
Publications: 2 Organism: Homo Sapiens
Tissue: Muscle, Skeletal Muscle
Pathways:AMPK Signaling, Circadian clock, MTOR Signaling
+ AMPKup-regulates activity img/direct-activation.png phosphorylation PPARGC1A 0.484
Identifier Residue Sequence Organism Cell Line
SIGNOR-209940 Ser539 SLFNVSPsCSSFNSP Homo sapiens
pmid sentence
AMPK can directly phosphorylate PGC-1a at Thr177 and Ser538 in in vitro assays PGC-1a phosphorylation might not directly affect its intrinsic coactivation activity, but, rather, release it from its repressor protein p160myb [79] and/or allow deacetylation and subsequent activation by SIRT1
Identifier Residue Sequence Organism Cell Line
SIGNOR-228646 Ser539 SLFNVSPsCSSFNSP Mus musculus
pmid sentence
AMPK phosphorylates PGC-1alpha directly both in vitro and in cells. These direct phosphorylations of the PGC-1alpha protein at threonine-177 and serine-538 are required for the PGC-1alpha-dependent induction of the PGC-1alpha promoter
Identifier Residue Sequence Organism Cell Line
SIGNOR-209936 Thr178 NHNHRIRtNPAIVKT Homo sapiens
pmid sentence
AMPK can directly phosphorylate PGC-1a at Thr177 and Ser538 in in vitro assays PGC-1a phosphorylation might not directly affect its intrinsic coactivation activity, but, rather, release it from its repressor protein p160myb [79] and/or allow deacetylation and subsequent activation by SIRT1
Identifier Residue Sequence Organism Cell Line
SIGNOR-228642 Thr178 NHNHRIRtNPAIVKT Mus musculus
pmid sentence
AMPK phosphorylates PGC-1alpha directly both in vitro and in cells. These direct phosphorylations of the PGC-1alpha protein at threonine-177 and serine-538 are required for the PGC-1alpha-dependent induction of the PGC-1alpha promoter
Publications: 4 Organism: Homo Sapiens, Mus Musculus
Tissue: Skeletal Muscle
Pathways:AMPK Signaling, Circadian clock, MTOR Signaling
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation SLC9A3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-277849 Ser555 AEGERRGsLAFIRSP Homo sapiens CACO-2 Cell
pmid sentence
AMPK activation by 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) phosphorylated NHE3 at S555. S555 is the primary site of phosphorylation by protein kinase A (PKA), but AMPK phosphorylated S555 independently of PKA. We conclude that AMPK activation inhibits NHE3 activity and NHE3 inhibition is associated with phosphorylation of NHE3 at S555 and S563.
Identifier Residue Sequence Organism Cell Line
SIGNOR-277848 Ser563 LAFIRSPsTDNVVNV Homo sapiens CACO-2 Cell
pmid sentence
AMPK activation by 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR) phosphorylated NHE3 at S555. S555 is the primary site of phosphorylation by protein kinase A (PKA), but AMPK phosphorylated S555 independently of PKA. We conclude that AMPK activation inhibits NHE3 activity and NHE3 inhibition is associated with phosphorylation of NHE3 at S555 and S563.
Publications: 2 Organism: Homo Sapiens
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation MLXIPL 0.407
Identifier Residue Sequence Organism Cell Line
SIGNOR-216526 Ser556 LLRSPGsPQETVPE Rattus norvegicus
pmid sentence
These results strongly suggested that the fatty acid inhibition of glucose-induced l-PK transcription resulted from AMPK phosphorylation of ChREBP at Ser568, which inactivated the DNA binding activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216561 Rattus norvegicus
pmid sentence
AMPK has also been suggested to phosphorylate the glucose-sensitive transcription factor ChREBP89 which dictates expression of an overlapping lipogenic gene signature with Srebp1
Publications: 2 Organism: Rattus Norvegicus
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation KCNA5 0.295
Identifier Residue Sequence Organism Cell Line
SIGNOR-273736 Ser559 VQRKVSGsRGSFCKA Homo sapiens HEK-293 Cell
pmid sentence
Thus, AMPK directly phosphorylates the α subunit of KV1.5 at Ser592 and, to a lesser extent, at Ser559. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-273735 Ser592 KCNVKAKsNVDLRRS Homo sapiens HEK-293 Cell
pmid sentence
Thus, AMPK directly phosphorylates the α subunit of KV1.5 at Ser592 and, to a lesser extent, at Ser559. 
Publications: 2 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation MCU 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-275548 Ser57 TVHQRIAsWQNLGAV in vitro
pmid sentence
Cellular ATP levels drop during early mitosis, and the mitochondrial Ca2+ transients boost mitochondrial respiration to restore energy homeostasis. This is achieved through mitosis-specific MCU phosphorylation and activation by the mitochondrial translocation of energy sensor AMP-activated protein kinase (AMPK). |In vitro kinase assays showed that AMPK immunoprecipitated from cells as well as recombinant AMPK phosphorylated MCU at Ser57
Publications: 1 Organism: In Vitro
+ AMPKup-regulates img/direct-activation.png phosphorylation KLC2 0.291
Identifier Residue Sequence Organism Cell Line
SIGNOR-216468 Ser582 PRMKRASsLNFLNKS Homo sapiens
pmid sentence
Consistent with phosphorylation of both ser545 and ser582 of klc2 contributing to its 14-3-3 binding, a ser545ala mutant of klc2 could be phosphorylated in vitro by ampk on ser582
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates img/direct-activation.png phosphorylation RAF1 0.302
Identifier Residue Sequence Organism Cell Line
SIGNOR-216616 Ser621 PKINRSAsEPSLHRA Homo sapiens
pmid sentence
Ampk also phosphorylated full-length, kinase-defective raf-1 (k375m) to generate two [32p]phosphopeptides, one co-migrating with synthetic tryptic peptide containing phospho-ser621 and the other with phospho-ser259
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation RRN3 0.286
Identifier Residue Sequence Organism Cell Line
SIGNOR-216592 Ser635 DTHFRSPsSSVGSPP Homo sapiens
pmid sentence
We show that ampk down-regulates rrna synthesis under glucose restriction by phosphorylating the rna polymerase i (pol i)-associated transcription factor tif-ia at a single serine residue (ser-635).
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation ACSS2 0.275
Identifier Residue Sequence Organism Cell Line
SIGNOR-271823 Ser659 PGLPKTRsGKIMRRV
pmid sentence
This translocation is mediated by AMP-activated protein kinase (AMPK)-dependent ACSS2 Ser659 phosphorylation and subsequent exposure of the nuclear localization signal of ACSS2 to KPNA1/importin α5 for binding. In the nucleus, ACSS2 forms a complex with TFEB (transcription factor EB) and utilizes the acetate generated from histone deacetylation to locally produce acetyl-CoA for histone acetylation in the promoter regions of TFEB target genes.
Publications: 1
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation CRY1 0.35
Identifier Residue Sequence Organism Cell Line
SIGNOR-216546 Ser71 ANLRKLNsRLFVIRG Homo sapiens
pmid sentence
Ampk was shown to regulate the stability of the core clock component cry1 though phosphorylation of cry1 ser71, which stimulates the direct binding of the fbox protein fbxl3 to cry1, targeting it for ubiquitin-mediated degradation
Publications: 1 Organism: Homo Sapiens
Pathways:Circadian clock
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation DNMT1 0.292
Identifier Residue Sequence Organism Cell Line
SIGNOR-264788 Ser714 DNIPEMPsPKKMHQG in vitro
pmid sentence
Together, these results indicate that AMPK phosphorylated DNMT1-Ser730, RBBP7-Ser314, and HAT1-Ser190|AMPK decreased DNMT1 activity
Publications: 1 Organism: In Vitro
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation RPTOR 0.502
Identifier Residue Sequence Organism Cell Line
SIGNOR-263046 Ser722 PRLRSVSsYGNIRAV Mus musculus
pmid sentence
These results suggest that AMPK activation can induce phosphorylation of both serine 722 and serine 792.|Raptor phosphorylation is required for inhibition of mTORC1 by AMPK
Identifier Residue Sequence Organism Cell Line
SIGNOR-263044 Ser792 LTQSAPAsPTNKGVH Mus musculus
pmid sentence
These results suggest that AMPK activation can induce phosphorylation of both serine 722 and serine 792.|Raptor phosphorylation is required for inhibition of mTORC1 by AMPK
Publications: 2 Organism: Mus Musculus
Pathways:MTOR Signaling
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation CFTR 0.398
Identifier Residue Sequence Organism Cell Line
SIGNOR-250350 Ser737 EPLERRLsLVPDSEQ Homo sapiens
pmid sentence
AMPK phosphorylates CFTR¬†in vitro¬†at two essential serines (Ser737and Ser768) in the R domain, formerly identified as "inhibitory" PKA sites.|Interestingly two of these sites, namely Ser737 and Ser768, have been identified as “inhibitory” R domain sites, i.e. when mutated to alanines they augment the open probability of CFTR relative to wild type|Our present results suggest that it might be AMPK rather than PKA that is phosphorylating Ser737 and Ser768 under baseline conditions
Identifier Residue Sequence Organism Cell Line
SIGNOR-72708 Ser768 LQARRRQsVLNLMTH Homo sapiens
pmid sentence
AMPK phosphorylates CFTR¬†in vitro¬†at two essential serines (Ser737and Ser768) in the R domain, formerly identified as "inhibitory" PKA sites.|Interestingly two of these sites, namely Ser737 and Ser768, have been identified as “inhibitory” R domain sites, i.e. when mutated to alanines they augment the open probability of CFTR relative to wild type|Our present results suggest that it might be AMPK rather than PKA that is phosphorylating Ser737 and Ser768 under baseline conditions
Publications: 2 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation FH 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-266313 Ser75 YGAQTVRsTMNFKIG in vitro
pmid sentence
Glucose deficiency induces AMPK activation, which phosphorylates FH at Ser75 and promotes its binding to ATF2 and the enrichment of the FH–ATF2 complex on the promoter regions of ATF2-targeted genes.
Publications: 1 Organism: In Vitro
Pathways:Citric acid cycle
+ AMPKup-regulates activity img/direct-activation.png phosphorylation ATG9A 0.251
Identifier Residue Sequence Organism Cell Line
SIGNOR-266365 Ser761 QSIPRSAsYPCAAPR Homo sapiens
pmid sentence
Our data suggest that the localization of mammalian Atg9A to autophagosomes requires phosphorylation on the C terminus of Atg9A at S761, which creates a 14-3-3ζ docking site. Under basal conditions, this phosphorylation is maintained at a low level and is dependent on both ULK1 and AMPK.
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation SLC12A2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-276630 Ser77 RPLGPTPsQSRFQVD in vitro
pmid sentence
 AMPK was found to directly phosphorylate a recombinant human-NKCC1 N-terminal fragment (1-293) with the phosphorylated site identified as S77. Mutation of Serine 77 to Alanine partially prevented the inhibitory effect of A-769662 on NKCC1 activity. In conclusion, AMPK can act to reduce NKCC1-mediated transport. 
Publications: 1 Organism: In Vitro
+ AMPKup-regulates quantity by stabilization img/direct-activation.png phosphorylation AMOTL1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-263105 Ser793 SSLRPARsVPSIAAA Homo sapiens HEK-293 Cell
pmid sentence
we show that AMPK directly phosphorylates S793 of AMOTL1. AMPK activation stabilizes and increases AMOTL1 steady-state protein levels, contributing to YAP inhibition.
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation NEDD4L 0.258
Identifier Residue Sequence Organism Cell Line
SIGNOR-277858 Ser795 VDLKPNGsEIMVTNE in vitro
pmid sentence
Expression of wild type Nedd4-2 or of Nedd4-2S795A lacking an AMPK phosphorylation consensus sequence downregulated Kir2.1 currents. The effect of wild type Nedd4-2 but not of Nedd4-2S795A was significantly augmented by additional coexpression of AMPK. 
Identifier Residue Sequence Organism Cell Line
SIGNOR-276326 Ser795 VDLKPNGsEIMVTNE in vitro
pmid sentence
The AMP-activated protein kinase (AMPK) down-regulates the inward rectifier K+ channel Kir2.1. Expression of wild type Nedd4-2 or of Nedd4-2S795A lacking an AMPK phosphorylation consensus sequence downregulated Kir2.1 currents. The effect of wild type Nedd4-2 but not of Nedd4-2S795A was significantly augmented by additional coexpression of AMPK.
Identifier Residue Sequence Organism Cell Line
SIGNOR-276324 Ser795 VDLKPNGsEIMVTNE in vitro
pmid sentence
The AMP-activated protein kinase (AMPK) down-regulates the inward rectifier K+ channel Kir2.1. Expression of wild type Nedd4-2 or of Nedd4-2S795A lacking an AMPK phosphorylation consensus sequence downregulated Kir2.1 currents. The effect of wild type Nedd4-2 but not of Nedd4-2S795A was significantly augmented by additional coexpression of AMPK.
Publications: 3 Organism: In Vitro
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation GYS1 0.479
Identifier Residue Sequence Organism Cell Line
SIGNOR-263102 Ser8 MPLNRTLsMSSLPGL in vitro
pmid sentence
Recombinant muscle GYS1 (glycogen synthase 1) and recombinant liver GYS2 were phosphorylated by recombinant AMPK (AMP-activated protein kinase) in a time-dependent manner and to a similar stoichiometry. The phosphorylation site in GYS2 was identified as Ser7, which lies in a favourable consensus for phosphorylation by AMPK. Phosphorylation of GYS1 or GYS2 by AMPK led to enzyme inactivation by decreasing the affinity for both UDP-Glc (UDP-glucose) [assayed in the absence of Glc-6-P (glucose-6-phosphate)] and Glc-6-P (assayed at low UDP-Glc concentrations).
Publications: 1 Organism: In Vitro
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation GYS2 0.397
Identifier Residue Sequence Organism Cell Line
SIGNOR-263101 Ser8 MLRGRSLsVTSLGGL in vitro
pmid sentence
Recombinant muscle GYS1 (glycogen synthase 1) and recombinant liver GYS2 were phosphorylated by recombinant AMPK (AMP-activated protein kinase) in a time-dependent manner and to a similar stoichiometry. The phosphorylation site in GYS2 was identified as Ser7, which lies in a favourable consensus for phosphorylation by AMPK. Phosphorylation of GYS1 or GYS2 by AMPK led to enzyme inactivation by decreasing the affinity for both UDP-Glc (UDP-glucose) [assayed in the absence of Glc-6-P (glucose-6-phosphate)] and Glc-6-P (assayed at low UDP-Glc concentrations).
Publications: 1 Organism: In Vitro
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation ACACA 0.542
Identifier Residue Sequence Organism Cell Line
SIGNOR-267475 Ser80 LHIRSSMsGLHLVKQ Homo sapiens
pmid sentence
Human ACC1 is inactivated by phosphorylation at Ser80, Ser1201 and Ser1216 by AMP-activated protein kinase (AMPK)
Publications: 1 Organism: Homo Sapiens
Pathways:Leptin Signaling
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation ACACA 0.542
Identifier Residue Sequence Organism Cell Line
SIGNOR-216655 Ser80 LHIRSSMsGLHLVKQ Homo sapiens
pmid sentence
Significant negative linear correlations between phospho-acc and acc activity were observed in all models (p < 0.01). The decline in acc activity was related to the decrease in pcr and the rise in amp. A relationship between phospho-ampk (threonine 172) and activity of ampk immunoprecipitated with anti-alpha(2) subunit antibody preparation was also observed.
Publications: 1 Organism: Homo Sapiens
Tissue: Muscle, Skeletal Muscle
Pathways:Leptin Signaling
+ AMPK img/unknown.png phosphorylation RB1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-216635 Ser811 IYISPLKsPYKISEG Homo sapiens
pmid sentence
Amp-activated protein kinase phosphorylates retinoblastoma protein. Rb phosphorylation sites, ser804 (ser811 in human), resembled the ampk consensus phosphorylation site.
Publications: 1 Organism: Homo Sapiens
Pathways:FLT3-ITD signaling
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation LIPE 0.378
Identifier Residue Sequence Organism Cell Line
SIGNOR-216507 Ser855 EPMRRSVsEAALAQP Homo sapiens
pmid sentence
Phosphorylation of bovine hormone-sensitive lipase by the amp-activated protein kinase.
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation YAP1 0.303
Identifier Residue Sequence Organism Cell Line
SIGNOR-277638 Ser94 RLRKLPDsFFKPPEP Homo sapiens HEK-293T Cell
pmid sentence
Moreover, AMPK directly phosphorylates YAP Ser 94, a residue essential for the interaction with TEAD, thus disrupting the YAP-TEAD interaction.
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates quantity by stabilization img/direct-activation.png phosphorylation TET2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-256135 Ser99 GGIKRTVsEPSLSGLL Homo sapiens Peripheral Blood Mononuclear Cell
pmid sentence
We identify the tumour suppressor TET2 as a substrate of the AMP-activated kinase (AMPK), which phosphorylates TET2 at serine 99, thereby stabilizing the tumour suppressor. Increased glucose levels impede AMPK-mediated phosphorylation at serine 99, which results in the destabilization of TET2 followed by dysregulation of both 5-hydroxymethylcytosine (5hmC) and the tumour suppressive function of TET2 in vitro and in vivo
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, IDH-TET in AML, NPM1_new
+ AMPKup-regulates activity img/direct-activation.png phosphorylation SCN5A 0.323
Identifier Residue Sequence Organism Cell Line
SIGNOR-275769 Thr101 IVLNKGKtIFRFSAT Homo sapiens Neuron
pmid sentence
Among the sites identified, only six were previously suggested to be the targets for specific kinases using in silico and/or in vitro analyses: S36 and S525 were attributed to the regulation by PKA; S484 and S664 were assigned to the serum- and glucocorticoid-inducible kinase 3 (SGK3); and S516 and S571 were ascribed to CaMKII (reviewed in Marionneau and Abriel, 2015). In marked contrast, several previously described phosphorylation sites were not detected in the present study, including the PKA-dependent S528, the CaMKII-associated T594, the PKC-dependent S1506, the adenosine monophosphate–activated protein kinase (AMPK)–dependent T101 (Liu et al., 2019), and the six Fyn-dependent tyrosines (Ahern et al., 2005; Iqbal et al., 2018).|The simplest interpretation of these findings is that these three phosphorylation clusters, at positions S457-S460, S483-T486, and S664-S671, are likely involved in regulating the basal and/or gating properties of native cardiac NaV1.5 channels. Conversely, the other phosphorylation sites, with lower stoichiometries, may play spatially or temporally distinct roles in the physiological or more pathophysiological regulation of channel expression or gating. | Remarkably, this MS analysis also revealed that the vast majority of identified phosphorylation sites (at least 26) are clustered, suggesting concomitant phosphorylation and roles in regulating channel expression and/or function. Unexpectedly, however, except for S664, S667, and S671, no apparent effects of phosphomimetic or phosphosilent mutations were observed on heterologously expressed (in HEK-293 cells) NaV1.5
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates quantity by stabilization img/direct-activation.png phosphorylation YBX2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-277868 Thr115 GFINRNDtKEDVFVH Mus musculus Brown Adipocyte
pmid sentence
In this context, YBX2 is a dual substrate for both AMPK and Akt2. The phosphorylation at Thr115 by AMPK or at Ser137 by Akt2 facilitates YBX2 accumulation in brown adipocytes by decreasing ubiquitination-mediated degradation. 
Publications: 1 Organism: Mus Musculus
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation GBF1 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-216588 Thr1337 GKIHRSAtDADVVNS Homo sapiens
pmid sentence
These results indicate that gbf1 is a novel ampk substrate and that the ampk-mediated phosphorylation of gbf1 at thr(1337) has a critical role, presumably by attenuating the function of gbf1, in the disassembly of the golgi apparatus induced under stress conditions that lower the intracellular atp concentration.
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates quantity by destabilization img/direct_inhibition.png phosphorylation SIRT7 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-275865 Thr153 TLTHMSItRLHEQKL
pmid sentence
Here, the authors show that energy stress induces an AMPK-dependent phosphorylation of Sirt7, which promotes its ubiquitin-independent degradation by REGγ, resulting in the down-regulation of rRNA transcription and cell survival.|These results strongly suggest that the phosphorylation status of SirT7 at T153 plays a crucial role in determining its subcellular distribution, degradation and binding to REGγ.
Publications: 1
+ AMPKup-regulates activity img/direct-activation.png phosphorylation PAQR3 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-273737 Thr32 PRGIRLYtYEQIPGS Homo sapiens HEK-293 Cell
pmid sentence
Firstly, PAQR3 functions as a scaffold protein that facilitates the formation of ATG14L- but not UVRAG-linked VPS34 complex, leading to elevated capacity of PI(3)P generation ahead of starvation signals. Secondly, AMPK phosphorylates PAQR3 at threonine 32 and switches on PI(3)P production to initiate autophagosome formation swiftly after glucose starvation. 
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation ALDH2 0.254
Identifier Residue Sequence Organism Cell Line
SIGNOR-271862 Thr356 GNPFDSKtEQGPQVD Mus musculus Macrophage
pmid sentence
Further studies demonstrate that in the absence of LDLR, AMPK phosphorylates ALDH2 at threonine 356 and enables its nuclear translocation. Nuclear ALDH2 interacts with HDAC3 and represses transcription of a lysosomal proton pump protein ATP6V0E2, critical for maintaining lysosomal function, autophagy, and degradation of oxidized low-density lipid protein.
Publications: 1 Organism: Mus Musculus
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation NOS3 0.266
Identifier Residue Sequence Organism Cell Line
SIGNOR-251618 Thr495 TGITRKKtFKEVANA Homo sapiens Vascular Endothelium
pmid sentence
The phosphorylation of both S617 and S635 have also been shown to promote increased eNOS-derived NO release (Michell et al., 2002). The phosphorylaiton of S617 can be induced by PKA or Akt activity, and may serve to sensitize eNOS to calmodulin binding and modulate the phosphorylation of other eNOS sites
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates img/direct-activation.png phosphorylation FOXO 0.398
Identifier Residue Sequence Organism Cell Line
SIGNOR-252886 Homo sapiens
pmid sentence
The energy sensor amp-activated protein kinase (ampk) has been shown to directly phosphorylate foxo factors at six regulatory sites that are distinct from the akt phosphorylation sites, resulting in foxo activation
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, IDH-TET in AML, FLT3-ITD signaling, NPM1_new
+ AMPKup-regulates img/direct-activation.png phosphorylation Gbeta 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-216471 Homo sapiens
pmid sentence
Ampk recruitment and h2b ser36 phosphorylation colocalized within genes activated by ampk-dependent pathways, both in promoters and in transcribed regions.
Publications: 1 Organism: Homo Sapiens
+ ULK1down-regulates img/direct_inhibition.png phosphorylation AMPK 0.47
Identifier Residue Sequence Organism Cell Line
SIGNOR-217484 Homo sapiens
pmid sentence
Ulk1/2 in turn phosphorylates all three subunits of ampk and thereby negatively regulates its activity.
Publications: 1 Organism: Homo Sapiens
Pathways:MTOR Signaling
+ PRKAG2form complex img/form-complex.png binding AMPK 0.737
Identifier Residue Sequence Organism Cell Line
SIGNOR-139176 Homo sapiens
pmid sentence
Gamma non-catalytic subunit mediates binding to amp, adp and atp, leading to activate or inhibit ampk: amp-binding results in allosteric activation of alpha catalytic subunit (prkaa1 or prkaa2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits.
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates activity img/direct_inhibition.png phosphorylation mTORC1 0.449
Identifier Residue Sequence Organism Cell Line
SIGNOR-216430 Homo sapiens HEK-293 Cell
pmid sentence
The phosphorylation of raptor by ampk is required for the inhibition of mtorc1 and cell-cycle arrest induced by energy stress.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216422 Homo sapiens
pmid sentence
A recent study revealed that ampk can inhibit mtorc1 independently of tsc2 by phosphorylating raptor at ser863.
Identifier Residue Sequence Organism Cell Line
SIGNOR-209862 Homo sapiens
pmid sentence
AMPK inhibits mTORC1 through two means: first, through phosphorylation of TSC2 to activate its GAP (GTPase-activating protein) activity that converts Rheb into an inactive GDP-bound state, thus switching off mitogenic stimulation of mTORC1 [31], and, secondly, through phosphorylation of raptor at Ser722 and Ser792, which leads to 14-3-3 protein binding and mTORC1 inhibition
Identifier Residue Sequence Organism Cell Line
SIGNOR-216418 Mus musculus MEF Cell
pmid sentence
AMP-activated protein kinase (AMPK), which is activated by LKB1/Strad/Mo25 upon high AMP levels, stimulates autophagy by inhibiting mTORC1.
Publications: 4 Organism: Homo Sapiens, Mus Musculus
Pathways:AMPK Signaling, Autophagy
+ AMPKup-regulates activity img/direct-activation.png phosphorylation WDR45 0.318
Identifier Residue Sequence Organism Cell Line
SIGNOR-268480 Homo sapiens U2-OS Cell
pmid sentence
WIPI4 is stimulated by AMPK, NUAK2 and BRSK2. This finding is supported by the results of our kinome screening, which identified AMPK and the AMKP-related kinases NUAK2 and BRSK2, all of which function downstream of LKB1 (ref. 69) and stimulate the localization of WIPI4 to nascent autophagosomes.
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation ULK1/Atg13/Fip200 0.41
Identifier Residue Sequence Organism Cell Line
SIGNOR-209913 Homo sapiens
pmid sentence
Under energy deprivation, AMPK positively regulates ULK1 to induce autophagy, with various studies revealing that AMPK binds to and phosphorylates ULK1
Publications: 1 Organism: Homo Sapiens
Pathways:AMPK Signaling, Autophagy
+ ULK2down-regulates img/direct_inhibition.png phosphorylation AMPK 0.306
Identifier Residue Sequence Organism Cell Line
SIGNOR-217487 Homo sapiens
pmid sentence
We could prove that ulk1-mediated phosphorylation of ampk reduced its level of phosphorylation at t172 of the _-subunit and hence interferes with its catalytic activity. I
Publications: 1 Organism: Homo Sapiens
+ glucosedown-regulates activity img/indirect_inhibition.png AMPK 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-256137 Mus musculus Myoblast
pmid sentence
Glucose restriction (GR) impaired differentiation of skeletal myoblasts and was associated with activation of the AMP-activated protein kinase (AMPK).
Publications: 1 Organism: Mus Musculus
Pathways:Acute Myeloid Leukemia, IDH-TET in AML
+ AMPKup-regulates img/indirect-activation.png Glycolysis 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-209929 Homo sapiens
pmid sentence
The decreased glycogen synthesis rates upon acute AMPK activation are generally coupled to an increase in the glycolytic flux, thanks to the activation of 6-phosphofructo-2-kinase (PFK-2) through direct phosphorylation on Ser466 [35]. PFK-2 catalyzes the synthesis of fructose 2,6-bisphosphate, a potent stimulator of glycolysis. Therefore, activation of AMPK rapidly mobilizes glucose into ATP-generating processes.
Publications: 1 Organism: Homo Sapiens
Pathways:AMPK Signaling
+ PRKAB1form complex img/form-complex.png binding AMPK 0.866
Identifier Residue Sequence Organism Cell Line
SIGNOR-139164 Homo sapiens
pmid sentence
Gamma non-catalytic subunit mediates binding to amp, adp and atp, leading to activate or inhibit ampk: amp-binding results in allosteric activation of alpha catalytic subunit (prkaa1 or prkaa2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits.
Publications: 1 Organism: Homo Sapiens
+ PRKAA1form complex img/form-complex.png binding AMPK 0.823
Identifier Residue Sequence Organism Cell Line
SIGNOR-139158 Homo sapiens Adipocyte
pmid sentence
Gamma non-catalytic subunit mediates binding to amp, adp and atp, leading to activate or inhibit ampk: amp-binding results in allosteric activation of alpha catalytic subunit (prkaa1 or prkaa2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits.
Publications: 1 Organism: Homo Sapiens
Tissue: Skeletal Muscle, Brain, Heart, Liver
+ glucosedown-regulates activity img/direct_inhibition.png chemical inhibition AMPK 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-277765 Homo sapiens
pmid sentence
Glucose deprivation, activates the glucose level–sensing kinase, AMPK, which in turn influences Rac1-dependent macropinocytosis. In this context macropinosomes take up necrotic cell debris as a rich nutrient source to fuel tumor cell growth
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, IDH-TET in AML
+ AMPKup-regulates activity img/direct-activation.png phosphorylation AMPK 0.796
Identifier Residue Sequence Organism Cell Line
SIGNOR-216411 Homo sapiens HEK-293 Cell
pmid sentence
Mutation of serine 108 to alanine, an autophosphorylation site within the glycogen binding domain of the beta1 subunit, almost completely abolishes activation of ampk by a-769662 in cells and in vitro, while only partially reducing activation by ampk
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, IDH-TET in AML, AMPK Signaling, Autophagy, Citric acid cycle, Circadian clock, FLT3-ITD signaling, Glycolysis and Gluconeogenesis, Leptin Signaling, MTOR Signaling, NPM1_new
+ AMPKdown-regulates activity img/direct_inhibition.png binding PRMT6 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-275889
pmid sentence
AMPK induces R388 hypomethylation by disrupting the association between PRMT6 and SIRT7.|Protein arginine methyltransferase 6 (PRMT6) directly interacts with and methylates SIRT7
Publications: 1
+ ATPdown-regulates img/direct_inhibition.png chemical inhibition AMPK 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-217481 Homo sapiens
pmid sentence
Atp promotes dephosphorylation of catalytic subunit, rendering the ampk enzyme inactive
Publications: 1 Organism: Homo Sapiens
+ STK11up-regulates img/direct-activation.png phosphorylation AMPK 0.596
Identifier Residue Sequence Organism Cell Line
SIGNOR-217469 in vitro
pmid sentence
We demonstrated that lkb1 phosphorylates ampk on the activation loop threonine (thr172) within the catalytic subunit and activates ampk in vitro. Here, we have investigated whether lkb1 corresponds to the major ampkk activity present in cell extracts. Ampkk purified from rat liver corresponds to lkb1, and blocking lkb1 activity in cells abolishes ampk activation in response to different stimuli
Identifier Residue Sequence Organism Cell Line
SIGNOR-217472 Homo sapiens
pmid sentence
We demonstrated that lkb1 phosphorylates ampk on the activation loop threonine (thr172) within the catalytic subunit and activates ampk in vitro. Here, we have investigated whether lkb1 corresponds to the major ampkk activity present in cell extracts. Ampkk purified from rat liver corresponds to lkb1, and blocking lkb1 activity in cells abolishes ampk activation in response to different stimuli
Publications: 2 Organism: In Vitro, Homo Sapiens
Pathways:AMPK Signaling, FLT3-ITD signaling
+ AMPKup-regulates activity img/direct-activation.png phosphorylation TSC 0.464
Identifier Residue Sequence Organism Cell Line
SIGNOR-217749 Mus musculus MEF Cell
pmid sentence
GSK3 inhibits the mTOR pathway by phosphorylating TSC2 in a manner dependent on AMPK-priming phosphorylation
Publications: 1 Organism: Mus Musculus
Pathways:AMPK Signaling, MTOR Signaling
+ PRKAB2form complex img/form-complex.png binding AMPK 0.855
Identifier Residue Sequence Organism Cell Line
SIGNOR-139167 Homo sapiens
pmid sentence
Gamma non-catalytic subunit mediates binding to amp, adp and atp, leading to activate or inhibit ampk: amp-binding results in allosteric activation of alpha catalytic subunit (prkaa1 or prkaa2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits.
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates activity img/indirect-activation.png RAC1 0.266
Identifier Residue Sequence Organism Cell Line
SIGNOR-277766 Homo sapiens Pancreatic Cancer Cell
pmid sentence
The discovery that AMPK activation is a general requirement for macropinosome formation in cancer cells dramatically extends our understanding of the regulation of this process. By increasing RAC1-GTP levels, AMPK activation stimulates macropinosome formation (Fig. 2I–K; Supplementary Fig. S5). 
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia
+ PRKAG3form complex img/form-complex.png binding AMPK 0.709
Identifier Residue Sequence Organism Cell Line
SIGNOR-139179 Homo sapiens
pmid sentence
Gamma non-catalytic subunit mediates binding to amp, adp and atp, leading to activate or inhibit ampk: amp-binding results in allosteric activation of alpha catalytic subunit (prkaa1 or prkaa2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits.
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation CRTC1 0.288
Identifier Residue Sequence Organism Cell Line
SIGNOR-216529 Homo sapiens
pmid sentence
Here we show that both ampk and calcineurin modulate longevity exclusively through post-translational modification of crtc-1, the sole c. elegans crtc. We demonstrate that crtc-1 is a direct ampk target.
Publications: 1 Organism: Homo Sapiens
+ PRKAG1form complex img/form-complex.png binding AMPK 0.756
Identifier Residue Sequence Organism Cell Line
SIGNOR-139170 Homo sapiens Adipocyte
pmid sentence
Gamma non-catalytic subunit mediates binding to amp, adp and atp, leading to activate or inhibit ampk: amp-binding results in allosteric activation of alpha catalytic subunit (prkaa1 or prkaa2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits.
Publications: 1 Organism: Homo Sapiens
Tissue: Skeletal Muscle, Brain, Heart, Liver
+ PRKACAdown-regulates activity img/direct_inhibition.png phosphorylation AMPK 0.388
Identifier Residue Sequence Organism Cell Line
SIGNOR-256111 Rattus norvegicus
pmid sentence
These agents also enhanced phosphorylation of alpha-Ser(485/491) by the cAMP-dependent protein kinase. AMPK alpha-Ser(485/491) phosphorylation was necessary but not sufficient for inhibition of AMPK activity in response to forskolin/isobutylmethylxanthine.
Publications: 1 Organism: Rattus Norvegicus
+ AMPKup-regulates img/direct-activation.png phosphorylation INSR 0.309
Identifier Residue Sequence Organism Cell Line
SIGNOR-216619 Homo sapiens
pmid sentence
Ampk phosphorylates and activates theinsulinreceptor, providing a direct link between ampk and theinsulin pathway.
Publications: 1 Organism: Homo Sapiens
Tissue: Muscle
Pathways:AMPK Signaling, Leptin Signaling, MTOR Signaling
+ AMPKdown-regulates activity img/indirect_inhibition.png MTOR 0.55
Identifier Residue Sequence Organism Cell Line
SIGNOR-260096 Homo sapiens
pmid sentence
AMPK inhibits the mTOR pathway through phosphorylation and activation of tuberous sclerosis protein 2 (TSC2) and causes direct activation of unc-51-like autophagy activating kinase 1 (ULK1) via phosphorylation of Ser555, thus promoting initiation of autophagy.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, IDH-TET in AML, FLT3-ITD signaling, Leptin Signaling, MTOR Signaling
+ AMPKup-regulates img/direct-activation.png phosphorylation TSC1 0.411
Identifier Residue Sequence Organism Cell Line
SIGNOR-216487 Homo sapiens
pmid sentence
Under energy starvation conditions, the amp-activated protein kinase (ampk) phosphorylates tsc2 and enhances its activity.
Identifier Residue Sequence Organism Cell Line
SIGNOR-216441 Homo sapiens
pmid sentence
Under energy starvation conditions, the amp-activated protein kinase (ampk) phosphorylates tsc2 and enhances its activity.
Publications: 2 Organism: Homo Sapiens
+ AMPKup-regulates img/direct-activation.png phosphorylation FOXO4 0.337
Identifier Residue Sequence Organism Cell Line
SIGNOR-216484 Homo sapiens
pmid sentence
The energy sensor amp-activated protein kinase (ampk) has been shown to directly phosphorylate foxo factors at six regulatory sites that are distinct from the akt phosphorylation sites, resulting in foxo activation
Publications: 1 Organism: Homo Sapiens
+ CAMK2Bup-regulates img/direct-activation.png phosphorylation AMPK 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-217493 Homo sapiens HeLa Cell
pmid sentence
These data indicate that the camkks function in intact cells as ampkks, predicting wider roles for these kinases in regulating ampk activity in vivo.
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates img/direct-activation.png phosphorylation FOXO1 0.355
Identifier Residue Sequence Organism Cell Line
SIGNOR-216478 Homo sapiens
pmid sentence
The energy sensor AMP-activated protein kinase (AMPK) has been shown to directly phosphorylate FoxO factors at six regulatory sites that are distinct from the Akt phosphorylation sites, resulting in FoxO activation.
Publications: 1 Organism: Homo Sapiens
Pathways:Leptin Signaling
+ Starvationup-regulates img/indirect-activation.png AMPK 0.7
Identifier Residue Sequence Organism Cell Line
SIGNOR-209796 Homo sapiens
pmid sentence
Starvation-induced autophagy is regulated by mitochondrial reactive oxygen species leading to AMPK activationSTARV
Identifier Residue Sequence Organism Cell Line
SIGNOR-209894 Homo sapiens Myotube
pmid sentence
L6 myotubes were incubated in serum-containing or serum-free medium for 3 h. Levels of phosphorylated AMPK, Akt, and ATM were greater in serum-starved cells than in control cells.
Publications: 2 Organism: Homo Sapiens
Pathways:AMPK Signaling, Autophagy
+ AMPKdown-regulates img/direct_inhibition.png phosphorylation HDAC4 0.275
Identifier Residue Sequence Organism Cell Line
SIGNOR-216658 Homo sapiens Neuron
pmid sentence
We show here that in liver, class iia hdacs (hdac4, 5, and 7) are phosphorylated and excluded from the nucleus by ampk family kinases.
Publications: 1 Organism: Homo Sapiens
Tissue: Muscle
+ AMPKup-regulates quantity img/indirect-activation.png NAMPT 0.275
Identifier Residue Sequence Organism Cell Line
SIGNOR-238824 Mus musculus
pmid sentence
Activated AMPK was required to promote GR-induced transcription of the NAD+ biosynthetic enzyme Nampt
Publications: 1 Organism: Mus Musculus
Pathways:Circadian clock
+ IL6up-regulates img/indirect-activation.png AMPK 0.248
Identifier Residue Sequence Organism Cell Line
SIGNOR-255338 Mus musculus
pmid sentence
AMPK phosphorylation was increased nearly fourfold (Fig. 2C) with the high dose of IL-6
Publications: 1 Organism: Mus Musculus
+ AMPKup-regulates activity img/indirect-activation.png MAPK14 0.284
Identifier Residue Sequence Organism Cell Line
SIGNOR-255951 Mus musculus
pmid sentence
P38 MAPK mediates the effect of AMPK on Gr induced transcriptional activity
Publications: 1 Organism: Mus Musculus
Pathways:FLT3-ITD signaling
+ CAMKK2up-regulates img/direct-activation.png phosphorylation AMPK 0.59
Identifier Residue Sequence Organism Cell Line
SIGNOR-217496 Homo sapiens HeLa Cell
pmid sentence
These data indicate that the camkks function in intact cells as ampkks, predicting wider roles for these kinases in regulating ampk activity in vivo.
Publications: 1 Organism: Homo Sapiens
+ PRKAA2form complex img/form-complex.png binding AMPK 0.824
Identifier Residue Sequence Organism Cell Line
SIGNOR-139161 Homo sapiens
pmid sentence
Gamma non-catalytic subunit mediates binding to amp, adp and atp, leading to activate or inhibit ampk: amp-binding results in allosteric activation of alpha catalytic subunit (prkaa1 or prkaa2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits.
Publications: 1 Organism: Homo Sapiens
+ AMPKdown-regulates quantity img/indirect_inhibition.png CPS1 0.274
Identifier Residue Sequence Organism Cell Line
SIGNOR-267920 Homo sapiens
pmid sentence
In KL cells, pharmacological activation of AMPK or expression of constitutively active AMPK reduced CPS1 mRNA and protein, and silencing AMPK increased CPS1 expression even in the presence of LKB1
Publications: 1 Organism: Homo Sapiens
+ ADPup-regulates img/direct-activation.png chemical activation AMPK 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-217475 Homo sapiens
pmid sentence
Amp binding to the gamma-regulatory domain promotes phosphorylation by the upstream kinase, protects the enzyme against dephosphorylation, as well as causing allosteric activation.Adp also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. Atp promotes dephosphorylation of catalytic subunit, rendering the ampk enzyme inactive.
Publications: 1 Organism: Homo Sapiens
+ ULK1/Atg13/Fip200down-regulates activity img/direct_inhibition.png phosphorylation AMPK 0.41
Identifier Residue Sequence Organism Cell Line
SIGNOR-209916 Homo sapiens
pmid sentence
Here we report that ulk1/2 in turn phosphorylates all three subunits of ampk and thereby negatively regulates its activity. Thus, we propose that ulk1 is not only involved in the induction of autophagy, but also in terminating signaling events that trigger autophagy. In our model, phosphorylation of ampk by ulk1 represents a negative feedback circuit.
Publications: 1 Organism: Homo Sapiens
Pathways:AMPK Signaling, Autophagy
+ STK11up-regulates activity img/direct-activation.png phosphorylation AMPK 0.596
Identifier Residue Sequence Organism Cell Line
SIGNOR-242602 in vitro
pmid sentence
We recently demonstrated that the LKB1 tumour suppressor kinase, in complex with the pseudokinase STRAD and the scaffolding protein MO25, phosphorylates and activates AMP_activated protein kinase (AMPK).
Publications: 1 Organism: In Vitro
Pathways:AMPK Signaling, FLT3-ITD signaling
+ TMIGD2up-regulates activity img/direct-activation.png phosphorylation AMPK 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-273665 Homo sapiens HEK-293 Cell
pmid sentence
 Upon activation by autophagy stimuli, IGPR-1 is phosphorylated at Ser220 via IKKβ. IGPR-1 acts as a pro-autophagy signaling receptor leading to activation of AMPK. 
Publications: 1 Organism: Homo Sapiens
+ acadesineup-regulates img/direct-activation.png chemical activation AMPK 0.8
Identifier Residue Sequence Organism Cell Line
SIGNOR-217478 Homo sapiens
pmid sentence
The activation of the ampk pathway by exendin-4 was induced by aicar, which was inhibited by compound c.
Publications: 1 Organism: Homo Sapiens
+ AMPKup-regulates activity img/direct-activation.png phosphorylation TET2 0.2
Identifier Residue Sequence Organism Cell Line
SIGNOR-260097 Homo sapiens U-937 Cell
pmid sentence
Inactivation of AMPK suppressed the expression of ten-eleven translocation methylcytosine dioxygenase 2 (TET2) in tumor cells. Compound C-induced AMPK suppression causes downregulation TET2 and FOXP3 expression, leading to death of parental and HQ-selected U937 cells. These results confirm the connection of AMPK with the TET2–FOXP3 axis in modulating the survival of AML cells and suggest that suppression of the AMPK–TET2–FOXP3 axis suppresses the progression of AML and HQ-induced malignant transformation of AML cells.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, IDH-TET in AML, NPM1_new
+ AMPK img/unknown.png phosphorylation AMPK 0.796
Identifier Residue Sequence Organism Cell Line
SIGNOR-216415 Homo sapiens HEK-293 Cell
pmid sentence
In contrast, the phosphorylation site mutations, ss24, 25aa and s182a, while having no effects on enzyme activity, are associated with nuclear redistribution of the subunit.
Publications: 1 Organism: Homo Sapiens
Pathways:Acute Myeloid Leukemia, IDH-TET in AML, AMPK Signaling, Autophagy, Citric acid cycle, Circadian clock, FLT3-ITD signaling, Glycolysis and Gluconeogenesis, Leptin Signaling, MTOR Signaling, NPM1_new
+ LEPRdown-regulates activity img/indirect_inhibition.png AMPK 0.384
Identifier Residue Sequence Organism Cell Line
SIGNOR-263510 Homo sapiens Pulmonary Artery Smooth Muscle Cell
pmid sentence
Leptin exerts an inhibitory effect on AMPK in the hypothalamus, thereby stimulating ACC and subsequently suppressing food intake.
Publications: 1 Organism: Homo Sapiens
Pathways:Leptin Signaling
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