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TKL1 transketolase TKL1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 856188, updated on 11-Apr-2024

Summary

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Gene symbol
TKL1
Gene description
transketolase TKL1
Primary source
SGD:S000006278
Locus tag
YPR074C
See related
AllianceGenome:SGD:S000006278
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables transketolase activity. Involved in pentose-phosphate shunt. Predicted to be located in cytoplasm. Predicted to be active in cytosol and nucleus. Orthologous to several human genes including TKT (transketolase) and TKTL2 (transketolase like 2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: XVI
Exon count:
1
Sequence:
Chromosome: XVI; NC_001148.4 (692796..694838, complement)

Chromosome XVI - NC_001148.4Genomic Context describing neighboring genes Neighboring gene CCR4-NOT core subunit NOT5 Neighboring gene tyrosine protein phosphatase LTP1 Neighboring gene Opy2p Neighboring gene uncharacterized protein

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Complementary function of two transketolase isoforms from Moniliella megachiliensis in relation to stress response. Iwata H, et al. AMB Express, 2017 Dec. PMID 28224439, Free PMC Article
  2. Structure and functioning mechanism of transketolase. Kochetov GA, et al. Biochim Biophys Acta, 2014 Sep. PMID 24929114
  3. Halogenated pyruvate derivatives as substrates of transketolase from Saccharomyces cerevisiae. Esakova OA, et al. Biochemistry (Mosc), 2009 Nov. PMID 19916939
  4. Effects of transketolase cofactors on its conformation and stability. Esakova OA, et al. Life Sci, 2005 Nov 19. PMID 16125202
  5. Binding of the coenzyme and formation of the transketolase active center. Kochetov G, et al. IUBMB Life, 2005 Jul. PMID 16081370

See all (117) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Transketolase is the key rate-limiting enzyme of the non-oxidative branch of the pentose phosphate pathway of carbohydrate transformation. (Review)
    Title: Structure and functioning mechanism of transketolase.
  2. characterization of NQM1 and TKL2, together with TAL1 and TKL1, regarding their roles in xylose utilization and fermentation
    Title: Characterization of non-oxidative transaldolase and transketolase enzymes in the pentose phosphate pathway with regard to xylose utilization by recombinant Saccharomyces cerevisiae.
  3. Halogenated pyruvate derivatives can function as donor substrates of transketolase.
    Title: Halogenated pyruvate derivatives as substrates of transketolase from Saccharomyces cerevisiae.
  4. In the presence of Ca2+, the active sites of TK1 and TK2 exhibit negative cooperativity in binding both xylulose-5-phosphate and ribose-5-phosphate and positive cooperativity in the catalytic transformation of the substrates.
    Title: Cooperative binding of substrates to transketolase from Saccharomyces cerevisiae.
  5. Review summarizes the results of studies on the kinetics of the interaction of thiamine diphosphate with transketolase and the generation of the catalytically active form of the coenzyme within the holoenzyme and formation of the enzyme's active center.
    Title: Binding of the coenzyme and formation of the transketolase active center.
  6. transketolase is regulated by donor substrates
    Title: Donor substrate regulation of transketolase.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables transketolase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transketolase activity IEA
Inferred from Electronic Annotation
more info
  
enables transketolase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in pentose-phosphate shunt IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in pentose-phosphate shunt IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in purine nucleotide metabolic process IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in cytoplasm IC
Inferred by Curator
more info
 PubMed 
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
transketolase TKL1
NP_015399.1
  • Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001148.4 Reference assembly

    Range
    692796..694838 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001184171.1 → NP_015399.1  TPA: transketolase TKL1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_015399.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W478, P23254
    UniProtKB/TrEMBL
    A0A387JUB2, A6ZWW1, B5VTM0, C7GY00, G2WPP6, N1NXM0
    Conserved Domains (1) summary
    TIGR00232
    Location:9 → 664
    tktlase_bact; transketolase, bacterial and yeast
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P23254.4

Gene LinkOut

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