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RFA2 Rfa2p [ Saccharomyces cerevisiae S288C ]

Gene ID: 855404, updated on 13-Apr-2024

Summary

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Gene symbol
RFA2
Gene description
Rfa2p
Primary source
SGD:S000005256
Locus tag
YNL312W
See related
AllianceGenome:SGD:S000005256
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
BUF1; RPA2; RPA32
Summary
Enables double-stranded DNA binding activity; sequence-specific DNA binding activity; and single-stranded DNA binding activity. Involved in several processes, including DNA metabolic process; DNA unwinding involved in DNA replication; and protein ubiquitination. Located in chromosome, telomeric region and condensed nuclear chromosome. Part of DNA replication factor A complex. Orthologous to several human genes including RPA2 (replication protein A2). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
chromosome: XIV
Exon count:
2
Sequence:
Chromosome: XIV; NC_001146.8 (48287..49216)

Chromosome XIV - NC_001146.8Genomic Context describing neighboring genes Neighboring gene Dal82p Neighboring gene tetratricopeptide repeat-containing protein EMW1 Neighboring gene putative SCF ubiquitin ligase complex subunit SKP2 Neighboring gene Zim17p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Monitoring Replication Protein A (RPA) dynamics in homologous recombination through site-specific incorporation of non-canonical amino acids. Pokhrel N, et al. Nucleic Acids Res, 2017 Sep 19. PMID 28934470, Free PMC Article
  2. Genome-wide screen identifies pathways that govern GAA/TTC repeat fragility and expansions in dividing and nondividing yeast cells. Zhang Y, et al. Mol Cell, 2012 Oct 26. PMID 22959270, Free PMC Article
  3. Essential functions of the 32 kDa subunit of yeast replication protein A. Dickson AM, et al. Nucleic Acids Res, 2009 Apr. PMID 19244309, Free PMC Article
  4. Mechanistic insight into the Cdc28-related protein kinase Ime2 through analysis of replication protein A phosphorylation. Clifford DM, et al. Cell Cycle, 2005 Dec. PMID 16294044
  5. Evidence of meiotic crossover control in Saccharomyces cerevisiae through Mec1-mediated phosphorylation of replication protein A. Bartrand AJ, et al. Genetics, 2006 Jan. PMID 16118184, Free PMC Article

See all (138) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. forms part of Replication Protein A (RPA) heterotrimer; dynamic DNA-binding domains promote the cooperative assembly of multiple RPAs on long ssDNA
    Title: A structural and dynamic model for the assembly of Replication Protein A on single-stranded DNA.
  2. Post-translational modification of the Rfa2 N-terminus is not required for cells to deal with DNA damage; however, it might influence whether cells proceed into M-phase in the continued presence of unrepaired DNA lesions.
    Title: The DNA damage response and checkpoint adaptation in Saccharomyces cerevisiae: distinct roles for the replication protein A2 (Rfa2) N-terminus.
  3. Results show that DNA damage response at eroded telomeres is characterized by the recruitment of Cdc13, replication protein A, DNA damage checkpoint proteins and the DNA repair protein Rad52 into a single focus.
    Title: The DNA damage response at eroded telomeres and tethering to the nuclear pore complex.
  4. Results suggests that RPA2 is necessary for structural integrity and may also be needed for intra- and/or inter-protein interactions.
    Title: Essential functions of the 32 kDa subunit of yeast replication protein A.
  5. Rfa2 serine 27 (S27) is required for Ime2-dependent Rfa2 phosphorylation in vivo.
    Title: Mechanistic insight into the Cdc28-related protein kinase Ime2 through analysis of replication protein A phosphorylation.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables double-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables single-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables single-stranded DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables telomeric DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in DNA recombination IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA topological change IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA unwinding involved in DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in cellular nitrogen compound biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in double-strand break repair via homologous recombination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in double-strand break repair via homologous recombination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in establishment of protein localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in heteroduplex formation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in macromolecule biosynthetic process IEA
Inferred from Electronic Annotation
more info
  
involved_in mitotic recombination IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in nucleic acid metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in nucleotide-excision repair IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in nucleotide-excision repair IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in nucleotide-excision repair IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein ubiquitination IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in reciprocal meiotic recombination IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in telomere maintenance via recombination IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in telomere maintenance via telomerase IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in telomere maintenance via telomere lengthening IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of DNA replication factor A complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of DNA replication factor A complex IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in chromosome, telomeric region IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in chromosome, telomeric region IMP
Inferred from Mutant Phenotype
more info
 PubMed 
located_in condensed nuclear chromosome IDA
Inferred from Direct Assay
more info
 PubMed 
located_in intracellular organelle IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
is_active_in site of double-strand break IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
Rfa2p
NP_014087.1
  • Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; in concert with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication s

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001146.8 Reference assembly

    Range
    48287..49216
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001183150.1 → NP_014087.1  TPA: Rfa2p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_014087.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W0N3, P26754, P38905
    UniProtKB/TrEMBL
    A6ZSG8, B3LPE6, C7GWQ9, C8ZGL2, G2WLF7, N1NZC3
    Conserved Domains (1) summary
    COG5235
    Location:1 → 273
    RFA2; Single-stranded DNA-binding replication protein A (RPA), medium (30 kD) subunit [DNA replication, recombination, and repair]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P26754.1

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