MCM6 MCM DNA helicase complex subunit MCM6 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: MCM6
Gene description: MCM DNA helicase complex subunit MCM6
Primary source: SGD:S000003169
Locus tag: YGL201C
See related: AllianceGenome:SGD:S000003169
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA replication origin binding activity and single-stranded DNA helicase activity. Contributes to 3'-5' DNA helicase activity and four-way junction helicase activity. Involved in DNA-dependent DNA replication and double-strand break repair via break-induced replication. Located in cytoplasm and nucleus. Part of MCM complex; MCM core complex; and nuclear lumen. Orthologous to human MCM6 (minichromosome maintenance complex component 6). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (117854..120907, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Sld3 binds specifically to DDK-phosphorylated peptides from two MCM subunits (Mcm4, 6) and then recruits Cdc45.
Title: Phosphopeptide binding by Sld3 links Dbf4-dependent kinase to MCM replicative helicase activation.
Mcm10 directly binds the Minichromosome Maintenance Complex via Mcm2 and the Mcm6 C terminus.
Title: Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex.
findings suggest a function for Cdt1p in promoting the assembly of the MCM complex and maintaining its integrity by interacting with Mcm6p
Title: Cdt1p, through its interaction with Mcm6p, is required for the formation, nuclear accumulation and chromatin loading of the MCM complex.
The minimal functional unit of Mcm4/6/7 is a hexamer.
Title: The effects of oligomerization on Saccharomyces cerevisiae Mcm4/6/7 function.
The Mcm ATPase active sites that flank Mcm2/5 (i.e. Mcm5/3 and 6/2) modulate the biochemical activities of the putative Mcm2/5 gate.
Title: The Saccharomyces cerevisiae Mcm6/2 and Mcm5/3 ATPase active sites contribute to the function of the putative Mcm2-7 'gate'.
DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA.
Title: Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.
An Mcm helicase acts as a checkpoint sensor for methyl methanesulfonate-induced DNA damage through direct binding to the replication checkpoint mediator Mrc1.
Title: The direct binding of Mrc1, a checkpoint mediator, to Mcm6, a replication helicase, is essential for the replication checkpoint against methyl methanesulfonate-induced stress.
The Mcm4/Mcm7 and Mcm4/Mcm6/Mcm7 assemblies can open to load onto circular DNA to initiate unwinding.
Title: Mcm subunits can assemble into two different active unwinding complexes.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to 3'-5' DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA helicase activity	IEA Inferred from Electronic Annotation more info
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA replication origin binding	IEA Inferred from Electronic Annotation more info
contributes_to four-way junction helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
contributes_to single-stranded 3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
contributes_to single-stranded DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA strand elongation involved in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA unwinding involved in DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA unwinding involved in DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA unwinding involved in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via break-induced replication	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IEA Inferred from Electronic Annotation more info
involved_in premeiotic DNA replication	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of CMG complex	IDA Inferred from Direct Assay more info	PubMed
part_of CMG complex	IEA Inferred from Electronic Annotation more info
part_of DNA replication preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
part_of MCM complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MCM complex	IDA Inferred from Direct Assay more info	PubMed
part_of MCM complex	IEA Inferred from Electronic Annotation more info
part_of MCM core complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of nuclear pre-replicative complex	IDA Inferred from Direct Assay more info	PubMed
part_of nuclear pre-replicative complex	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed
part_of replication fork protection complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: MCM DNA helicase complex subunit MCM6

NP_011314.2

EC 3.6.4.12

Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

117854..120907 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181066.1 → NP_011314.2 TPA: MCM DNA helicase complex subunit MCM6 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011314.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTV3, P53091, Q870M9

UniProtKB/TrEMBL

A0A8H4C1R7, C7GWZ4

Conserved Domains (3) summary

smart00350
Location:272 → 836

MCM; minichromosome maintenance proteins

pfam14551
Location:112 → 205

MCM_N; MCM N-terminal domain

pfam18263
Location:900 → 1007

MCM6_C; MCM6 C-terminal winged-helix domain