CDC7 serine/threonine protein kinase CDC7 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: CDC7
Gene description: serine/threonine protein kinase CDC7
Primary source: SGD:S000002175
Locus tag: YDL017W
See related: AllianceGenome:SGD:S000002175
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: LSD6; SAS1
Summary: Enables protein serine/threonine kinase activity. Involved in several processes, including DNA-dependent DNA replication; protein phosphorylation; and regulation of cell cycle process. Located in chromosome, centromeric region and nucleus. Part of Dbf4-dependent protein kinase complex. Colocalizes with chromatin. Orthologous to human CDC7 (cell division cycle 7). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (424209..425732)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer.
Title: The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer.
Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase.
Title: Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase.
Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast.
Title: Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast.
Cdc7p regulates replication-coupled histone modification via a CAC1-dependent mechanism involving H4 K16ac deposition.
Title: Modulation of Gene Silencing by Cdc7p via H4 K16 Acetylation and Phosphorylation of Chromatin Assembly Factor CAF-1 in Saccharomyces cerevisiae.
we confirmed that this release indeed accompanies the dissociation of acetylated Smc3 as well as Rec8 from meiotic chromosomes during late prophase-I. This release requires, in addition to PLK, the cohesin regulator, Wapl (Rad61/Wpl1 in yeast), and Dbf4-dependent Cdc7 kinase (DDK)
Title: Meiosis-specific prophase-like pathway controls cleavage-independent release of cohesin by Wapl phosphorylation.
main conclusion from our studies is that Cdc7 kinase is found at many locations in the genome, but is enriched at functional origins of replication
Title: O Cdc7 kinase where art thou?
The authors propose that the timing of Eco1 phosphorylation, and potentially that of other DDK substrates, is determined in part by sequestration of DDK at unfired replication origins during S phase.
Title: Firing of Replication Origins Frees Dbf4-Cdc7 to Target Eco1 for Destruction.
Cdc7 is loosely bound to chromatin during G1. At the G1/S transition, Cdc7 binding to chromatin is increased and stabilized, preferentially at sites that may become replication origins.
Title: Localization of Cdc7 Protein Kinase During DNA Replication in Saccharomyces cerevisiae.
Data show that Dbf4-dependent kinase (DDK)-phosphoryled MCM DNA helicase complex subunit MCM2 preferentially interacts with DNA replication initiation factor CDC45
Title: Conserved mechanism for coordinating replication fork helicase assembly with phosphorylation of the helicase.
Study provides evidence that a key prerequisite for double-strand breaks formation-phosphorylation of Mer2 by Dbf4-dependent Cdc7 kinase, DDK-is linked to replication via association of the kinase with replisome components.
Title: Temporospatial coordination of meiotic DNA replication and recombination via DDK recruitment to replisomes.

Submit: New GeneRIF Correction See all GeneRIFs (29)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in mitotic DNA replication checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic DNA replication preinitiation complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in mitotic DNA replication preinitiation complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of exit from mitosis	IPI Inferred from Physical Interaction more info	PubMed
involved_in peptidyl-serine phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of meiosis I	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of meiotic DNA double-strand break formation involved in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein localization to kinetochore	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of spindle attachment to meiosis I kinetochore	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of spindle attachment to meiosis I kinetochore	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in premeiotic DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein-containing complex localization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein-containing complex localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of Dbf4-dependent protein kinase complex	IDA Inferred from Direct Assay more info	PubMed
part_of Dbf4-dependent protein kinase complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of Dbf4-dependent protein kinase complex	IPI Inferred from Physical Interaction more info	PubMed
part_of chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: serine/threonine protein kinase CDC7

NP_010267.3

EC 2.7.11.1

S/T kinase and catalytic subunit of DDK (Dbf4-dependent kinase); required for origin firing and replication initiation; phosphorylates MCM subunits and Cdc45 to drive assembly of the pre-IC and the CMG helicase; maintains Cse4p at kinetochores through phosphorylation to regulate chromosome segregation, regulates pre-meiotic replication, meiotic DSB formation, monopolar attachment of homologs during MI through monopolin recruitment and middle meiotic gene expression; HsCDC7 and DBF4 complement

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

424209..425732

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180076.3 → NP_010267.3 TPA: serine/threonine protein kinase CDC7 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010267.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRX3, P06243

UniProtKB/TrEMBL

A0A8H8UMQ7, B5VFP7, N1PAB0

Conserved Domains (2) summary

cd14019
Location:31 → 348

STKc_Cdc7; Catalytic domain of the Serine/Threonine Kinase, Cell Division Cycle 7 kinase

cl21453
Location:443 → 476

PKc_like; Protein Kinases, catalytic domain