RFA1 replication factor A subunit protein RFA1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: RFA1
Gene description: replication factor A subunit protein RFA1
Primary source: SGD:S000000065
Locus tag: YAR007C
See related: AllianceGenome:SGD:S000000065
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: BUF2; FUN3; RPA1; RPA70
Summary: Enables double-stranded DNA binding activity; sequence-specific DNA binding activity; and single-stranded DNA binding activity. Involved in several processes, including DNA metabolic process; DNA unwinding involved in DNA replication; and protein ubiquitination. Located in chromosome, telomeric region; condensed nuclear chromosome; and cytosol. Part of DNA replication factor A complex. Orthologous to human RPA1 (replication protein A1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: I

Exon count:: 1

Sequence:: Chromosome: I; NC_001133.9 (156754..158619, complement)

Chromosome I - NC_001133.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001133.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Single molecule microscopy reveals key physical features of repair foci in living cells.
Title: Single molecule microscopy reveals key physical features of repair foci in living cells.
DNA asymmetry promotes SUMO modification of the single-stranded DNA-binding protein RPA.
Title: DNA asymmetry promotes SUMO modification of the single-stranded DNA-binding protein RPA.
RPA-mediated recruitment of Bre1 couples histone H2B ubiquitination to DNA replication and repair.
Title: RPA-mediated recruitment of Bre1 couples histone H2B ubiquitination to DNA replication and repair.
Complementary roles of Pif1 helicase and single stranded DNA binding proteins RPA and DNA polymerase subunits in stimulating DNA replication through G-quadruplexes have been demonstrated.
Title: Complementary roles of Pif1 helicase and single stranded DNA binding proteins in stimulating DNA replication through G-quadruplexes.
These results support a model in which Rtt105 functions as an RPA chaperone that escorts RPA to the nucleus and facilitates its loading onto ssDNA at replication forks.
Title: Rtt105 functions as a chaperone for replication protein A to preserve genome stability.
dynamic rearrangements of DNA-binding domains during coordinated RPA binding regulated by phosphorylation at S178
Title: A structural and dynamic model for the assembly of Replication Protein A on single-stranded DNA.
Overexpression of Rad51 was also found to suppress the rfa1 BIR defects. We suggest that Rad51 binding to the ssDNA formed by excessive end resection and during D-loop migration can partially compensate for dysfunctional RPA.
Title: RPA Stabilization of Single-Stranded DNA Is Critical for Break-Induced Replication.
RPA-dependent recruitment of Mec1-Ddc2 is crucial for maintaining its homodimeric state at ssDNA and that Ddc2's recruitment domain and CCD are important for Mec1-dependent survival of UV-light-induced DNA damage.
Title: Structural Basis of Mec1-Ddc2-RPA Assembly and Activation on Single-Stranded DNA at Sites of Damage.
Force regulated dynamics of RPA1 on a DNA fork during DNA replication has been reported.
Title: Force regulated dynamics of RPA on a DNA fork.
secondary structures within single-stranded DNA are potent instigators of genome instability, and RPA and Mre11-Sae2 play important roles in preventing their formation and propagation, respectively.
Title: Mre11-Sae2 and RPA Collaborate to Prevent Palindromic Gene Amplification.

Submit: New GeneRIF Correction See all GeneRIFs (29)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	HDA more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA topological change	IDA Inferred from Direct Assay more info	PubMed
involved_in DNA unwinding involved in DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular nitrogen compound biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via homologous recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in establishment of protein localization	IPI Inferred from Physical Interaction more info	PubMed
involved_in heteroduplex formation	IDA Inferred from Direct Assay more info	PubMed
involved_in macromolecule biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in mitotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nucleic acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in nucleotide-excision repair	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_positive_effect positive regulation of DNA helicase activity	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_positive_effect positive regulation of endodeoxyribonuclease activity	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_positive_effect positive regulation of endodeoxyribonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IPI Inferred from Physical Interaction more info	PubMed
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance via telomerase	IPI Inferred from Physical Interaction more info	PubMed
involved_in telomere maintenance via telomere lengthening	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of DNA replication factor A complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	IMP Inferred from Mutant Phenotype more info	PubMed
located_in condensed nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: replication factor A subunit protein RFA1

NP_009404.1

Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved ssDNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; promotes recruitment, helicase and nuclease activities of Dna2p; role in DNA catenation/decatenation pathway of chromosome disentangling; relocalizes to the cytosol in response to hypoxia