UBI4 ubiquitin [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: UBI4
Gene description: ubiquitin
Primary source: SGD:S000003962
Locus tag: YLL039C
See related: AllianceGenome:SGD:S000003962
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: SCD2; UB14
Summary: Enables protein tag. Involved in protein ubiquitination. Colocalizes with peroxisome. Orthologous to several human genes including UBC (ubiquitin C). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (64062..65207, complement)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Systematic profiling of dominant ubiquitin variants reveals key functional nodes contributing to evolutionary selection.
Title: Systematic profiling of dominant ubiquitin variants reveals key functional nodes contributing to evolutionary selection.
A novel recognition site for polyubiquitin and ubiquitin-like signals in an unexpected region of proteasomal subunit Rpn1.
Title: A novel recognition site for polyubiquitin and ubiquitin-like signals in an unexpected region of proteasomal subunit Rpn1.
Programmed ubiquitin acetylation using genetic code expansion reveals altered ubiquitination patterns.
Title: Programmed ubiquitin acetylation using genetic code expansion reveals altered ubiquitination patterns.
These results indicate that ubiquitination, a modification well known to target membrane proteins to the lysosome or vacuole for degradation, can also function as recycling signal to sort a SNARE into COPI vesicles in a non-degradative pathway.
Title: COPI mediates recycling of an exocytic SNARE by recognition of a ubiquitin sorting signal.
ubi4 repeats modulate proteostasis and stress survival
Title: Variable repeats in the eukaryotic polyubiquitin gene ubi4 modulate proteostasis and stress survival.
Both Ssa1 and Ssa2 are essential for the ubiquitin-dependent degradation of short-lived proteins and the requirements of Ssa proteins and the co-chaperones widely vary depending on the conformations and folding status of the substrates.
Title: The requirements of yeast Hsp70 of SSA family for the ubiquitin-dependent degradation of short-lived and abnormal proteins.
Zap1 controls UBI4 by inducing transcription from an intragenic promoter, and the resulting truncated mRNA encodes only two of the five ubiquitin repeats.
Title: Activation of the Yeast UBI4 Polyubiquitin Gene by Zap1 Transcription Factor via an Intragenic Promoter Is Critical for Zinc-deficient Growth.
study provided evidence that S65 phosphorylation of ubiquitin is a biologically important modification that influences nearly every step of the in vivo ubiquitin life cycle
Title: Phosphorylation of ubiquitin at Ser65 affects its polymerization, targets, and proteome-wide turnover.
Expression of UbQ2N-E64G in stress hypersensitive SUB60 cells decreased growth rate and prolonged half-life of substrates of UFD pathway, besides failure of complementation under heat and antibiotic stresses, so Gln2 and Glu64 have been conserved.
Title: Q2N and E64G double mutation of ubiquitin confers a stress sensitive phenotype on Saccharomyces cerevisiae.
Ubiquitin signals proteolysis-independent stripping of transcription factors.
Title: Ubiquitin signals proteolysis-independent stripping of transcription factors.

Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables protein tag activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein tag activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in modification-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ubiquitin

NP_013061.1

Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via ubiquitin-26S proteasome system; essential for cellular stress response; encoded as polyubiquitin precursor comprised of 5 head-to-tail repeats; number of repeats is evolutionarily unstable within and between yeast species; protein abundance increases in response to DNA replication stress; implicated as modulator of replicative lifespan and meiotic recombination

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

64062..65207 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181859.1 → NP_013061.1 TPA: ubiquitin [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013061.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXW7, P0CG63

UniProtKB/TrEMBL

A0A8H4BZ78, A7A0L9, B3LTD4, C8ZCT5, G2WIF8

Conserved Domains (1) summary

cd01803
Location:77 → 152

Ubl_ubiquitin; ubiquitin-like (Ubl) domain found in ubiquitin