Pdpk1 3-phosphoinositide dependent protein kinase-1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Pdpk1provided by RGD
Full Name: 3-phosphoinositide dependent protein kinase-1provided by RGD
Primary source: RGD:620307
See related: Ensembl:ENSRNOG00000006136 AllianceGenome:RGD:620307
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables 3-phosphoinositide-dependent protein kinase activity; insulin receptor binding activity; and protein kinase binding activity. Involved in cellular response to brain-derived neurotrophic factor stimulus; focal adhesion assembly; and negative regulation of neuron apoptotic process. Located in cytosol; perikaryon; and plasma membrane. Human ortholog(s) of this gene implicated in prostate cancer. Orthologous to several human genes including PDPK1 (3-phosphoinositide dependent protein kinase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Thymus (RPKM 176.6), Brain (RPKM 142.6) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q12

Exon count:: 17

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (13610000..13687226, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (13105435..13182664, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (13446373..13525248, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PDK1 upregulates PINK1-mediated pulmonary endothelial cell mitophagy during hypoxia-induced pulmonary vascular remodeling.
Title: PDK1 upregulates PINK1-mediated pulmonary endothelial cell mitophagy during hypoxia-induced pulmonary vascular remodeling.
A PDK-1 allosteric agonist neutralizes insulin signaling derangements and beta-amyloid toxicity in neuronal cells and in vitro.
Title: A PDK-1 allosteric agonist neutralizes insulin signaling derangements and beta-amyloid toxicity in neuronal cells and in vitro.
M1 and M2 mAChRs activate PDK1 and regulate PKC betaI and epsilon and the exocytotic apparatus at the NMJ.
Title: M(1) and M(2) mAChRs activate PDK1 and regulate PKC βI and ε and the exocytotic apparatus at the NMJ.
Long noncoding Mirt2 reduces apoptosis to alleviate myocardial infarction through regulation of the miR-764/PDK1 axis.
Title: Long noncoding Mirt2 reduces apoptosis to alleviate myocardial infarction through regulation of the miR-764/PDK1 axis.
STRAP reduces endoplasmic reticulum stress and apoptosis in cardiomyocytes and attenuates myocardial ischemia-reperfusion injury by activating PI3K/PDK1/Akt signaling pathway.
Title: STRAP reduces endoplasmic reticulum stress and apoptosis in cardiomyocytes and attenuates myocardial ischemia-reperfusion injury by activating PI3K/PDK1/Akt signaling pathway.
PI3K, PDK1, and PKCzeta have roles independent of insulin, in inducing glucose uptake
Title: Neuregulin signaling on glucose transport in muscle cells.
Thrombin activation of PI3K/PDK1/Akt signaling promotes cyclin D1 upregulation and retinal pigment epithelium cell proliferation.
Title: Thrombin activation of PI3K/PDK1/Akt signaling promotes cyclin D1 upregulation and RPE cell proliferation.
PDK1 has a protective role in the injured ischemic myocardium via overexpression of the cell survival pathway in cardiomyocyte
Title: Cardioprotective effects of 3-phosphoinositide-dependent protein kinase-1 on hypoxic injury in cultured neonatal rat cardiomyocytes and myocardium in a rat myocardial infarct model.
PDK-1 plays a permissive role in alpha(1B)-adrenoceptor desensitization and phosphorylation
Title: Roles of phosphoinositide-dependent kinase-1 in α1B-adrenoceptor phosphorylation and desensitization.
Amelioration of vascular endothelium dysfunction by insulin may be due to stimulation of phosphoinositide dependent kinase and Akt pathways.
Title: Beneficial effect of insulin in hyperhomocysteinemia and diabetes mellitus-induced vascular endothelium dysfunction: role of phosphoinositide dependent kinase and protein kinase B.

Submit: New GeneRIF Correction See all GeneRIFs (16)

General gene information

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Markers

D10Rat258 (e-PCR), detects polymorphism
- UniSTS:226367

D10Arb29 (e-PCR)
- UniSTS:239688

BF417221 (e-PCR)
- UniSTS:249672

BF397060 (e-PCR)
- UniSTS:246098

Pdpk1 (e-PCR), detects polymorphism
- UniSTS:532427

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 3-phosphoinositide-dependent protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables 3-phosphoinositide-dependent protein kinase activity	IEA Inferred from Electronic Annotation more info
enables 3-phosphoinositide-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info
enables 3-phosphoinositide-dependent protein kinase activity	TAS Traceable Author Statement more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables insulin receptor binding	IDA Inferred from Direct Assay more info	PubMed
enables phospholipase activator activity	IEA Inferred from Electronic Annotation more info
enables phospholipase activator activity	ISO Inferred from Sequence Orthology more info
enables phospholipase binding	IEA Inferred from Electronic Annotation more info
enables phospholipase binding	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	IDA Inferred from Direct Assay more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in calcium-mediated signaling	IEA Inferred from Electronic Annotation more info
involved_in calcium-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in cell migration	IEA Inferred from Electronic Annotation more info
involved_in cell migration	ISO Inferred from Sequence Orthology more info
involved_in cellular response to brain-derived neurotrophic factor stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to epidermal growth factor stimulus	IEA Inferred from Electronic Annotation more info
involved_in cellular response to epidermal growth factor stimulus	ISO Inferred from Sequence Orthology more info
involved_in cellular response to insulin stimulus	ISO Inferred from Sequence Orthology more info
involved_in epidermal growth factor receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in epidermal growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in extrinsic apoptotic signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in extrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in focal adhesion assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hyperosmotic response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within hyperosmotic response	ISO Inferred from Sequence Orthology more info
involved_in insulin receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in insulin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in insulin-like growth factor receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in insulin-like growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cardiac muscle cell apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cardiac muscle cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of endothelial cell apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of endothelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of toll-like receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of toll-like receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transforming growth factor beta receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-serine phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of blood vessel endothelial cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of blood vessel endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein localization to plasma membrane	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of protein localization to plasma membrane	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of release of sequestered calcium ion into cytosol	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of release of sequestered calcium ion into cytosol	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of sprouting angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of vascular endothelial cell proliferation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of vascular endothelial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in regulation of canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
involved_in regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in regulation of endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in regulation of mast cell degranulation	IEA Inferred from Electronic Annotation more info
involved_in regulation of mast cell degranulation	ISO Inferred from Sequence Orthology more info
involved_in type B pancreatic cell development	IEA Inferred from Electronic Annotation more info
involved_in type B pancreatic cell development	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cell projection	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in focal adhesion	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in perikaryon	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	TAS Traceable Author Statement more info
located_in postsynaptic density	IEA Inferred from Electronic Annotation more info
located_in postsynaptic density	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: 3-phosphoinositide-dependent protein kinase 1

Names: pkB kinase; protein kinase B kinase

NP_112343.2

EC 2.7.11.1

XP_006246098.1

EC 2.7.11.1

XP_038942864.1

EC 2.7.11.1

XP_038942865.1

EC 2.7.11.1

XP_063126017.1

EC 2.7.11.1

XP_063126018.1

EC 2.7.11.1

XP_063126019.1

EC 2.7.11.1

XP_063126020.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031081.2 → NP_112343.2 3-phosphoinositide-dependent protein kinase 1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000010

UniProtKB/Swiss-Prot

G3V9W3, O55173

UniProtKB/TrEMBL

A0A8I5ZS05

Related

ENSRNOP00000061683.1, ENSRNOT00000067660.2

Conserved Domains (2) summary

cd01262
Location:444 → 550

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cd05581
Location:83 → 345

STKc_PDK1; Catalytic domain of the Serine/Threonine Kinase, Phosphoinositide-dependent kinase 1

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

13610000..13687226 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039086936.2 → XP_038942864.1 3-phosphoinositide-dependent protein kinase 1 isoform X1

UniProtKB/TrEMBL

A0A8I5ZS05

Conserved Domains (2) summary

cd01262
Location:417 → 523

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cd05581
Location:56 → 318

STKc_PDK1; Catalytic domain of the Serine/Threonine Kinase, Phosphoinositide-dependent kinase 1
XM_006246036.4 → XP_006246098.1 3-phosphoinositide-dependent protein kinase 1 isoform X1

UniProtKB/TrEMBL

A0A8I5ZS05

Conserved Domains (2) summary

cd01262
Location:417 → 523

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cd05581
Location:56 → 318

STKc_PDK1; Catalytic domain of the Serine/Threonine Kinase, Phosphoinositide-dependent kinase 1
XM_063269948.1 → XP_063126018.1 3-phosphoinositide-dependent protein kinase 1 isoform X2
XM_063269947.1 → XP_063126017.1 3-phosphoinositide-dependent protein kinase 1 isoform X1
XM_039086937.2 → XP_038942865.1 3-phosphoinositide-dependent protein kinase 1 isoform X5

Conserved Domains (2) summary

cd01262
Location:225 → 331

PH_PDK1; 3-Phosphoinositide dependent protein kinase 1 (PDK1) pleckstrin homology (PH) domain

cl21453
Location:1 → 126

PKc_like; Protein Kinases, catalytic domain
XM_063269950.1 → XP_063126020.1 3-phosphoinositide-dependent protein kinase 1 isoform X4
XM_063269949.1 → XP_063126019.1 3-phosphoinositide-dependent protein kinase 1 isoform X3