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Nt5c2 5'-nucleotidase, cytosolic II [ Mus musculus (house mouse) ]

Gene ID: 76952, updated on 21-Apr-2024

Summary

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Official Symbol
Nt5c2provided by MGI
Official Full Name
5'-nucleotidase, cytosolic IIprovided by MGI
Primary source
MGI:MGI:2178563
See related
Ensembl:ENSMUSG00000025041 AllianceGenome:MGI:2178563
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Gmp; CnII; Nt5b; Pnt5; cN-II; Gm9751; 2010002I23Rik
Summary
Enables GMP 5'-nucleotidase activity. Involved in GMP catabolic process to guanine; allantoin metabolic process; and dGMP catabolic process. Located in cytosol. Is expressed in several structures, including alimentary system; integumental system; nervous system; respiratory system; and sensory organ. Human ortholog(s) of this gene implicated in hereditary spastic paraplegia 45. Orthologous to human NT5C2 (5'-nucleotidase, cytosolic II). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in whole brain E14.5 (RPKM 17.2), CNS E14 (RPKM 14.7) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
19 C3; 19 38.97 cM
Exon count:
27
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (46873829..47003613, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (46885390..47015174, complement)

Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E8753 Neighboring gene arsenite methyltransferase Neighboring gene cyclin M2 Neighboring gene STARR-seq mESC enhancer starr_46325 Neighboring gene STARR-seq mESC enhancer starr_46328 Neighboring gene CapStarr-seq enhancer MGSCv37_chr19:46962419-46962620 Neighboring gene DBF4 homolog pseudogene Neighboring gene STARR-positive B cell enhancer ABC_E11036 Neighboring gene CapStarr-seq enhancer MGSCv37_chr19:47044836-47045019 Neighboring gene STARR-seq mESC enhancer starr_46332 Neighboring gene STARR-seq mESC enhancer starr_46336 Neighboring gene lysophosphatidic acid receptor 1 pseudogene Neighboring gene STARR-positive B cell enhancer ABC_E11037 Neighboring gene STARR-seq mESC enhancer starr_46338 Neighboring gene internexin neuronal intermediate filament protein, alpha Neighboring gene polycomb group ring finger 6 Neighboring gene predicted gene, 23140

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Deoxyribonucleotide phosphatase from rat liver and cultured mouse fibroblasts. Magnusson G. Eur J Biochem, 1971 May 28. PMID 5560407
  2. Clonal evolution mechanisms in NT5C2 mutant-relapsed acute lymphoblastic leukaemia. Tzoneva G, et al. Nature, 2018 Jan 25. PMID 29342136, Free PMC Article
  3. Effects of genetic deletion of soluble 5'-nucleotidases NT5C1A and NT5C2 on AMPK activation and nucleotide levels in contracting mouse skeletal muscles. Kviklyte S, et al. Am J Physiol Endocrinol Metab, 2017 Jul 1. PMID 28325731
  4. Bovine cytosolic IMP/GMP-specific 5'-nucleotidase: cloning and expression of active enzyme in Escherichia coli. Allegrini S, et al. Biochem J, 1997 Dec 1. PMID 9371705, Free PMC Article
  5. Cloning of a mouse cytosolic 5'-nucleotidase-I identifies a new gene related to human autoimmune infertility-related protein. Sala-Newby GB, et al. Biochim Biophys Acta, 2001 Oct 31. PMID 11690631

See all (31) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The results on muscles from the NT5C knockout mice contradict previous findings where AMP levels and AMPK activity were shown to be modulated by NT5C enzymes.
    Title: Effects of genetic deletion of soluble 5'-nucleotidases NT5C1A and NT5C2 on AMPK activation and nucleotide levels in contracting mouse skeletal muscles.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (2)  1 citation
  • Endonuclease-mediated (3) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables 5'-nucleotidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 5'-nucleotidase activity ISO
Inferred from Sequence Orthology
more info
 PubMed 
enables ATP binding ISO
Inferred from Sequence Orthology
more info
  
enables GMP 5'-nucleotidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables GMP 5'-nucleotidase activity ISO
Inferred from Sequence Orthology
more info
 PubMed 
enables IMP 5'-nucleotidase activity ISO
Inferred from Sequence Orthology
more info
 PubMed 
enables XMP 5'-nucleosidase activity IEA
Inferred from Electronic Annotation
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleoside phosphotransferase activity ISO
Inferred from Sequence Orthology
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables ubiquitin protein ligase activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in GMP catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in GMP catabolic process to guanine IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in GMP metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in IMP catabolic process ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in IMP metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in IMP metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in adenosine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in adenosine metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in allantoin metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in allantoin metabolic process ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in amide catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in amide catabolic process ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in dGMP catabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in dGMP metabolic process ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of defense response to virus by host ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within nucleotide metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in protein K48-linked ubiquitination ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
is_active_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytosol ISO
Inferred from Sequence Orthology
more info
 PubMed 
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
cytosolic purine 5'-nucleotidase
Names
cytosolic 5'-nucleotidase II
cytosolic nucleoside phosphotransferase 5'N
NP_001157835.1
NP_001157837.1
NP_001363995.1
NP_001363997.1
NP_001363998.1
NP_001363999.1
NP_084086.3
XP_030106990.1
XP_030106991.1
XP_030106997.1
XP_030107000.1
XP_036017617.1
XP_036017618.1
XP_036017619.1
XP_036017620.1
XP_036017621.1
XP_036017622.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001164363.2 → NP_001157835.1  cytosolic purine 5'-nucleotidase isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1).
    Source sequence(s)
    AC108814, AC122442
    Consensus CDS
    CCDS50462.1
    UniProtKB/TrEMBL
    E9Q9M1
    Related
    ENSMUSP00000130898.3, ENSMUST00000172239.3
    Conserved Domains (1) summary
    pfam05761
    Location:69 → 514
    5_nucleotid; 5' nucleotidase family

  2. NM_001164365.2 → NP_001157837.1  cytosolic purine 5'-nucleotidase isoform 2

    See identical proteins and their annotated locations for NP_001157837.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is shorter than isoform 1.
    Source sequence(s)
    AC108814, AC122442
    Consensus CDS
    CCDS50461.1
    UniProtKB/TrEMBL
    A0A494B9X3
    Related
    ENSMUSP00000157802.2, ENSMUST00000235234.2
    Conserved Domains (1) summary
    pfam05761
    Location:68 → 513
    5_nucleotid; 5' nucleotidase family

  3. NM_001377066.1 → NP_001363995.1  cytosolic purine 5'-nucleotidase isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5), as well as variants 3, 6, and 7, encodes isoform 3.
    Source sequence(s)
    AC108814, AC122442
    UniProtKB/Swiss-Prot
    Q3V1L4, Q6P223, Q8BZ43, Q9D8G6
    Conserved Domains (1) summary
    pfam05761
    Location:43 → 488
    5_nucleotid; 5' nucleotidase family

  4. NM_001377068.1 → NP_001363997.1  cytosolic purine 5'-nucleotidase isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (6), as well as variants 3, 5, and 7, encodes isoform 3.
    Source sequence(s)
    AC108814, AC122442
    UniProtKB/Swiss-Prot
    Q3V1L4, Q6P223, Q8BZ43, Q9D8G6
    Conserved Domains (1) summary
    pfam05761
    Location:43 → 488
    5_nucleotid; 5' nucleotidase family

  5. NM_001377069.1 → NP_001363998.1  cytosolic purine 5'-nucleotidase isoform 3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (7), as well as variants 3, 5, and 6, encodes isoform 3.
    Source sequence(s)
    AC108814, AC122442
    UniProtKB/Swiss-Prot
    Q3V1L4, Q6P223, Q8BZ43, Q9D8G6
    Related
    ENSMUSP00000158280.2, ENSMUST00000236501.2
    Conserved Domains (1) summary
    pfam05761
    Location:43 → 488
    5_nucleotid; 5' nucleotidase family

  6. NM_001377070.1 → NP_001363999.1  cytosolic purine 5'-nucleotidase isoform 4

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442
    Related
    ENSMUSP00000158576.2, ENSMUST00000236727.2
    Conserved Domains (1) summary
    pfam05761
    Location:14 → 459
    5_nucleotid; 5' nucleotidase family

  7. NM_029810.5 → NP_084086.3  cytosolic purine 5'-nucleotidase isoform 3

    See identical proteins and their annotated locations for NP_084086.3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Variants 3 and 5-7 all encode the same isoform (3).
    Source sequence(s)
    AC108814, AC122442
    Consensus CDS
    CCDS29883.1
    UniProtKB/Swiss-Prot
    Q3V1L4, Q6P223, Q8BZ43, Q9D8G6
    Related
    ENSMUSP00000129126.2, ENSMUST00000168536.9
    Conserved Domains (1) summary
    pfam05761
    Location:43 → 488
    5_nucleotid; 5' nucleotidase family

RNA

  1. NR_028353.2 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  2. NR_165058.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  3. NR_165059.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  4. NR_165060.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  5. NR_165061.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  6. NR_165062.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  7. NR_165063.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  8. NR_165064.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  9. NR_165065.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  10. NR_165066.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  11. NR_165067.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  12. NR_165068.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  13. NR_165069.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  14. NR_165070.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  15. NR_165071.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  16. NR_165072.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  17. NR_165073.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  18. NR_165074.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  19. NR_165075.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  20. NR_165076.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442

  21. NR_165253.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC108814, AC122442
    Related
    ENSMUST00000086961.15

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000085.7 Reference GRCm39 C57BL/6J

    Range
    46873829..47003613 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036161729.1 → XP_036017622.1  cytosolic purine 5'-nucleotidase isoform X3

    Conserved Domains (1) summary
    cl17687
    Location:1 → 297
    5_nucleotid; 5' nucleotidase family

  2. XM_030251137.1 → XP_030106997.1  cytosolic purine 5'-nucleotidase isoform X3

    Conserved Domains (1) summary
    cl17687
    Location:1 → 297
    5_nucleotid; 5' nucleotidase family

  3. XM_036161725.1 → XP_036017618.1  cytosolic purine 5'-nucleotidase isoform X2

    Conserved Domains (1) summary
    pfam05761
    Location:55 → 357
    5_nucleotid; 5' nucleotidase family

  4. XM_036161727.1 → XP_036017620.1  cytosolic purine 5'-nucleotidase isoform X2

    Conserved Domains (1) summary
    pfam05761
    Location:55 → 357
    5_nucleotid; 5' nucleotidase family

  5. XM_030251130.2 → XP_030106990.1  cytosolic purine 5'-nucleotidase isoform X2

    Conserved Domains (1) summary
    pfam05761
    Location:55 → 357
    5_nucleotid; 5' nucleotidase family

  6. XM_030251140.2 → XP_030107000.1  cytosolic purine 5'-nucleotidase isoform X3

    Conserved Domains (1) summary
    cl17687
    Location:1 → 297
    5_nucleotid; 5' nucleotidase family

  7. XM_036161724.1 → XP_036017617.1  cytosolic purine 5'-nucleotidase isoform X1

    UniProtKB/Swiss-Prot
    Q3V1L4, Q6P223, Q8BZ43, Q9D8G6
    Conserved Domains (1) summary
    pfam05761
    Location:43 → 488
    5_nucleotid; 5' nucleotidase family

  8. XM_030251131.1 → XP_030106991.1  cytosolic purine 5'-nucleotidase isoform X2

    Conserved Domains (1) summary
    pfam05761
    Location:55 → 357
    5_nucleotid; 5' nucleotidase family

  9. XM_036161728.1 → XP_036017621.1  cytosolic purine 5'-nucleotidase isoform X3

    Conserved Domains (1) summary
    cl17687
    Location:1 → 297
    5_nucleotid; 5' nucleotidase family

  10. XM_036161726.1 → XP_036017619.1  cytosolic purine 5'-nucleotidase isoform X2

    Conserved Domains (1) summary
    pfam05761
    Location:55 → 357
    5_nucleotid; 5' nucleotidase family
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q3V1L4.2 GenPept UniProtKB/Swiss-Prot:Q3V1L4

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