Nr0b1 nuclear receptor subfamily 0, group B, member 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nr0b1provided by RGD
Official Full Name: nuclear receptor subfamily 0, group B, member 1provided by RGD
Primary source: RGD:62028
See related: Ensembl:ENSRNOG00000003765 AllianceGenome:RGD:62028
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Ahch; DAX-1
Summary: Enables double-stranded DNA binding activity and sequence-specific DNA binding activity. Involved in regulation of transcription, DNA-templated; response to peptide hormone; and spermatogenesis. Located in cytoplasm and nucleus. Human ortholog(s) of this gene implicated in 46,XY sex reversal 2 and X-linked adrenal hypoplasia congenita. Orthologous to human NR0B1 (nuclear receptor subfamily 0 group B member 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Adrenal (RPKM 151.7) and Testes (RPKM 71.1) See more
Orthologs: human mouse all
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Genomic context

Location:: Xq21

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	X	NC_086039.1 (54707658..54711786)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	X	NC_051356.1 (50756886..50761014)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	X	NC_005120.4 (54734385..54738513)

Chromosome X - NC_086039.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Heat stress upregulates aromatases expression through nuclear DAX-1 deficiency in R2C Leydig tumor cells.
Title: Heat stress upregulates aromatases expression through nuclear DAX-1 deficiency in R2C Leydig tumor cells.
Hepatocyte-Specific Deficiency of DAX-1 Protects Mice from Acetaminophen-Induced Hepatotoxicity by Activating NRF2 Signaling.
Title: Hepatocyte-Specific Deficiency of DAX-1 Protects Mice from Acetaminophen-Induced Hepatotoxicity by Activating NRF2 Signaling.
In conclusion, the presented data revealed that the pituitary Nr5a1/Ctnnb1/Nr0b1 system is controlled by endogenous Gnrh network activity, and hypothalamic-derived regulatory inputs can affect the processes responsible for gene transcription, translation, and protein intracellular translocation.
Title: Gonadotropin-releasing hormone and kisseptin-10 regulate nuclear receptor subfamily 5 group a member 1/catenin beta 1/ nuclear receptor subfamily 0 group B member 1 activity in female rat anterior pituitary gland.
High fat diet aggravates BDE47-inhibited testosterone production through hyperinsulinemia, and the accumulation of testicular BDE47 that induces the up-regulation of DAX-1 and the subsequent down-regulation of steroidogenic proteins.
Title: High-fat diet aggravates 2,2',4,4'-tetrabromodiphenyl ether-inhibited testosterone production via DAX-1 in Leydig cells in rats.
In vivo blockade of testicular alpha1-adrenergic receptors changed the stress-induced decrease of Nur77 and increase of Arr19 and Dax1 in Leydig cells.
Title: Intratesticular alpha1-adrenergic receptors mediate stress-disturbed transcription of steroidogenic stimulator NUR77 as well as steroidogenic repressors DAX1 and ARR19 in Leydig cells of adult rats.
These results suggest that the low level of DAX-1 may enhance the transcriptional activity of SF-1 and expression of genes related to glucocorticoid synthesis, which are targeted by SF-1, in adrenals from SHR.
Title: Characteristic induction of steroidogenic factor 1 (SF-1) and DAX-1 and enhanced expression of glucocorticoid synthesis-related genes in adrenals from spontaneously hypertensive rats.
DAX-1 acts as a novel corepressor of liver X receptor alpha and inhibits hepatic lipogenesis
Title: Orphan nuclear receptor DAX-1 acts as a novel corepressor of liver X receptor alpha and inhibits hepatic lipogenesis.
Inhibin A is capable of feedback antagonizing FSH action on GCs by reducing FSHR expression at ovarian level via a short feedback loop. Transcriptional factor receptors, such as SF-1, AR and DAX-1 were involved in this regulation.
Title: Inhibin A inhibits follicle-stimulating hormone (FSH) action by suppressing its receptor expression in cultured rat granulosa cells.
Dexamethasone increases expression of DAX-1, which results in increased amounts of complexed DAX-1-SF-1, in the absence of any change in the expression of SF-1.
Title: In vivo inhibition of steroidogenic acute regulatory protein expression by dexamethasone parallels induction of the negative transcription factor DAX-1.
Nr0b1 negatively regulates steroid synthesis through the inhibition of StAR expression and indicate that the absence of Nr0b1 in R2C cells is, at least in part, responsible for the constitutive steroidogenesis and StAR expression observed.
Title: Regulation of steroidogenesis and steroidogenic acute regulatory protein in R2C cells by DAX-1 (dosage-sensitive sex reversal, adrenal hypoplasia congenita, critical region on the X chromosome, gene-1).

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General gene information

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Markers

UniSTS:464683 (e-PCR)
- UniSTS:464683

Nr0b1 (e-PCR), detects polymorphism
- UniSTS:464683

Nr0b1 (e-PCR), detects polymorphism
- UniSTS:257080

Nr0b1 (e-PCR), detects polymorphism
- UniSTS:525926

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables AF-2 domain binding	ISO Inferred from Sequence Orthology more info
enables DNA binding	TAS Traceable Author Statement more info	PubMed
enables DNA hairpin binding	IEA Inferred from Electronic Annotation more info
enables DNA hairpin binding	ISO Inferred from Sequence Orthology more info
enables DNA hairpin binding	ISS Inferred from Sequence or Structural Similarity more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	ISO Inferred from Sequence Orthology more info
enables RNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables double-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables nuclear receptor binding	IEA Inferred from Electronic Annotation more info
enables nuclear receptor binding	ISO Inferred from Sequence Orthology more info
enables protein domain specific binding	IEA Inferred from Electronic Annotation more info
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein homodimerization activity	IEA Inferred from Electronic Annotation more info
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables sequence-specific DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
NOT enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables transcription corepressor activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription corepressor activity	IEA Inferred from Electronic Annotation more info
enables transcription corepressor activity	ISO Inferred from Sequence Orthology more info
enables transcription factor binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in Leydig cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within Leydig cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in Sertoli cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within Sertoli cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in adrenal gland development	IBA Inferred from Biological aspect of Ancestor more info
involved_in adrenal gland development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within adrenal gland development	ISO Inferred from Sequence Orthology more info
involved_in adrenal gland development	ISO Inferred from Sequence Orthology more info
involved_in adrenal gland development	ISS Inferred from Sequence or Structural Similarity more info
involved_in endodermal cell differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within endodermal cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in gonad development	ISO Inferred from Sequence Orthology more info
involved_in gonad development	ISS Inferred from Sequence or Structural Similarity more info
involved_in male gonad development	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within male gonad development	ISO Inferred from Sequence Orthology more info
involved_in male gonad development	ISO Inferred from Sequence Orthology more info
involved_in male sex determination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within male sex determination	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-binding transcription factor activity	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-templated transcription	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of intracellular steroid hormone receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of intracellular steroid hormone receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of steroid biosynthetic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of negative regulation of steroid biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of negative regulation of steroid biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in protein localization	IEA Inferred from Electronic Annotation more info
involved_in protein localization	ISO Inferred from Sequence Orthology more info
involved_in regulation of steroid biosynthetic process	TAS Traceable Author Statement more info	PubMed
involved_in response to benzoic acid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to immobilization stress	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to immobilization stress	ISO Inferred from Sequence Orthology more info
involved_in response to organic substance	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to peptide hormone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to retinoic acid	HEP more info	PubMed
involved_in response to retinoic acid	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within sex determination	ISO Inferred from Sequence Orthology more info
involved_in spermatogenesis	IBA Inferred from Biological aspect of Ancestor more info
involved_in spermatogenesis	IEA Inferred from Electronic Annotation more info
involved_in spermatogenesis	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within spermatogenesis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in centriolar satellite	IEA Inferred from Electronic Annotation more info
located_in centriolar satellite	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in nuclear speck	IEA Inferred from Electronic Annotation more info
located_in nuclear speck	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
located_in ribosome	IEA Inferred from Electronic Annotation more info
located_in ribosome	ISO Inferred from Sequence Orthology more info
located_in ribosome	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: nuclear receptor subfamily 0 group B member 1

Names: adrenal hypoplasia, congenital homolog; nuclear receptor DAX-1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053317.2 → NP_445769.2 nuclear receptor subfamily 0 group B member 1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000021

UniProtKB/Swiss-Prot

P70503, Q63152

UniProtKB/TrEMBL

A6IPW7, G3V6H3

Related

ENSRNOP00000005023.2, ENSRNOT00000005023.5

Conserved Domains (2) summary

cd07350
Location:233 → 466

NR_LBD_Dax1; The ligand binding domain of DAX1 protein, a nuclear receptor lacking DNA binding domain

pfam14046
Location:1 → 47

NR_Repeat; Nuclear receptor repeat