PSMB4 proteasome 20S subunit beta 4 [Homo sapiens (human)] - Gene

Summary

Official Symbol: PSMB4provided by HGNC
Official Full Name: proteasome 20S subunit beta 4provided by HGNC
Primary source: HGNC:HGNC:9541
See related: Ensembl:ENSG00000159377 MIM:602177; AllianceGenome:HGNC:9541
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: HN3; HsN3; PRAAS3; PROS26; PROS-26
Summary: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in placenta (RPKM 102.8), lymph node (RPKM 94.8) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 1q21.3

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2023_10	current	GRCh38.p14 (GCF_000001405.40)	1	NC_000001.11 (151399573..151401937)
RS_2023_10	current	T2T-CHM13v2.0 (GCF_009914755.1)	1	NC_060925.1 (150523282..150525646)
105.20220307	previous assembly	GRCh37.p13 (GCF_000001405.25)	1	NC_000001.10 (151372049..151374413)

Chromosome 1 - NC_000001.11 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Bassoon inhibits proteasome activity via interaction with PSMB4.
Title: Bassoon inhibits proteasome activity via interaction with PSMB4.
FoxM1 transcriptionally activates the PSMB4 expression level in cervical cancer cells.
Title: A novel FoxM1-PSMB4 axis contributes to proliferation and progression of cervical cancer.
High PSMB4 expression is associated with breast cancer.
Title: PSMB4 overexpression enhances the cell growth and viability of breast cancer cells leading to a poor prognosis.
PSMB4 knockdown decreased the expression levels of MMP2, MMP9 and cathepsin B and decreased proliferation, migration and invasion abilities in human GBM cells.
Title: Interference with PSMB4 Expression Exerts an Anti-Tumor Effect by Decreasing the Invasion and Proliferation of Human Glioblastoma Cells.
We further identified miR-148b as a negative regulator of proteasome beta-4 subunit. Enforced expression of miR-148b resulted in vitro growth inhibition of melanoma cells, whereas this inhibition was further abolished by enforced expression of proteasome beta-4 subunit
Title: Proteasome beta-4 subunit contributes to the development of melanoma and is regulated by miR-148b.
Our findings implied that PSMB4 is involved in the progression of EOC (epithelial ovarian cancer) and could serve as potential therapeutical target of EOC. These data suggested that PSMB4 may promote cell proliferation via the NF-kappaB-target gene in EOC (epithelial ovarian cancer)
Title: PSMB4 expression associates with epithelial ovarian cancer growth and poor prognosis.
Taken together, our results demonstrated that PSMB4 regulated MM cell growth in part by activating NF-kappaB-miR-21 signaling, which may represent promising targets for novel specific therapies.
Title: PSMB4 promotes multiple myeloma cell growth by activating NF-κB-miR-21 signaling.
Data indicate that recruitment of proteasomes into mutant huntingtin (mHTT) protein initiated inclusion bodies (IBs) was observed when the PSMB4-GFP was co-expressed with mHTT.
Title: Dynamic recruitment of active proteasomes into polyglutamine initiated inclusion bodies.
proteasome subunit beta type 4 (PSMB4), the beta7 subunit of the 20S core complex, was identified as a direct binding partner of CRBN.
Title: Cereblon inhibits proteasome activity by binding to the 20S core proteasome subunit beta type 4.
While 26 proteasome dysfunction is observed in Parkinson's disease (PD), diverse mutations in the parkin gene are linked to early-onset autosomal-recessive forms of familial PD.
Title: Parkin directly modulates 26S proteasome activity.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Associated conditions

Description	Tests
Proteasome-associated autoinflammatory syndrome 3 MedGen: C4747850 OMIM: 617591 GeneReviews: Not available	Compare labs

EBI GWAS Catalog

Description
Genome-wide association study identifies loci affecting blood copper, selenium and zinc. EBI GWAS Catalog EBI GWAS CatalogPubMed

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Nef	nef	HIV-1 Nef was found to markedly downregulate intracellular levels of both a co-expressed HsN3 and the endogenous simian homologue, suggesting Nef may alter proteasome function in infected cells	PubMed
	nef	Amino acids 34-143 of HIV-1 Nef mediate its binding to amino acids 73-249 of the HsN3 proteasomal subunit	PubMed
Tat	tat	HIV-1 Tat slightly enhances the activity of the purified 26 S proteasome	PubMed
	tat	Amino acids Lys51, Arg52, and Asp67 of HIV-1 Tat represent the proteasome binding site of Tat, and Tat amino acids 37-72 are necessary for proteasomal interaction and suppression of 11 S regulator-mediated antigen presentation	PubMed
	tat	HIV-1 Tat inhibits the peptidase activity of the 20 S proteasome and interferes with the formation of the 20 S proteasome-11 S regulator complex	PubMed
	tat	HIV-1 Tat binds to the alpha2, alpha4, alpha6, alpha7, beta1, beta2, beta3, beta5, beta6, beta7, LMP7/beta5i, and MECL1/beta2i subunits of the proteasome 20 S core structure and can inhibit cellular proteasome function	PubMed
Vif	vif	HIV-1 Vif binds to the cellular cytidine deaminase APOBEC3G and targets it for degradation through an interaction with the proteasome, thereby inhibiting APOBEC3G mediated restriction of HIV-1 replication	PubMed
integrase	gag-pol	Proteasomal degradation of HIV-1 integrase in mammalian cells occurs by the N-end rule pathway	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

ECD06181 (e-PCR)
- UniSTS:287243

MARC_13789-13790:1006804989:3 (e-PCR)
- UniSTS:267555

MARC_13789-13790:1025290513:1 (e-PCR)
- UniSTS:267555

stSG627163 (e-PCR)
- UniSTS:457256

ECD05441 (e-PCR)
- UniSTS:286507

ECD10374 (e-PCR)
- UniSTS:291413

G54029 (e-PCR)
- UniSTS:109411

REN33974 (e-PCR)
- UniSTS:358774

REN33975 (e-PCR)
- UniSTS:358775

REN33976 (e-PCR)
- UniSTS:358776

REN33977 (e-PCR)
- UniSTS:358777

REN33978 (e-PCR)
- UniSTS:358778

REN33979 (e-PCR)
- UniSTS:358779

REN33980 (e-PCR)
- UniSTS:358780

REN33981 (e-PCR)
- UniSTS:358781

REN33982 (e-PCR)
- UniSTS:358782

REN33983 (e-PCR)
- UniSTS:358783

REN33984 (e-PCR)
- UniSTS:358784

REN33985 (e-PCR)
- UniSTS:358785

REN33986 (e-PCR)
- UniSTS:358786

REN33987 (e-PCR)
- UniSTS:358787

REN33988 (e-PCR)
- UniSTS:358788

REN33989 (e-PCR)
- UniSTS:358789

REN33990 (e-PCR)
- UniSTS:358790

REN33991 (e-PCR)
- UniSTS:358791

REN33992 (e-PCR)
- UniSTS:358792

REN33993 (e-PCR)
- UniSTS:358793

ECD22511 (e-PCR)
- UniSTS:303497

ECD10980 (e-PCR)
- UniSTS:292017

RH64651 (e-PCR)
- UniSTS:35030

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables lipopolysaccharide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of inflammatory response to antigenic stimulus	IEA Inferred from Electronic Annotation more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	NAS Non-traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in ciliary basal body	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	TAS Traceable Author Statement more info
located_in extracellular exosome	HDA more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	TAS Traceable Author Statement more info	PubMed
part_of proteasome complex	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome complex	NAS Non-traceable Author Statement more info	PubMed
part_of proteasome core complex	IDA Inferred from Direct Assay more info	PubMed
part_of proteasome core complex	ISS Inferred from Sequence or Structural Similarity more info
part_of proteasome core complex, beta-subunit complex	IBA Inferred from Biological aspect of Ancestor more info
part_of proteasome core complex, beta-subunit complex	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: proteasome subunit beta type-4

Names: 26 kDa prosomal protein; hsBPROS26; macropain beta chain; multicatalytic endopeptidase complex beta chain; proteasome (prosome, macropain) subunit, beta type, 4; proteasome beta chain; proteasome chain 3; proteasome subunit HsN3; proteasome subunit beta 4; proteasome subunit beta7; proteasome subunit, beta type, 4; testis tissue sperm-binding protein Li 79P

NP_002787.2

EC 3.4.25.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_002796.3 → NP_002787.2 proteasome subunit beta type-4

See identical proteins and their annotated locations for NP_002787.2

Status: REVIEWED

Source sequence(s)

AL589764

Consensus CDS

CCDS996.1

UniProtKB/Swiss-Prot

B2R9L3, P28070, P31148, Q5SZS5, Q6IBI4, Q969L6

UniProtKB/TrEMBL

A0A140VK46

Related

ENSP00000290541.6, ENST00000290541.7

Conserved Domains (1) summary

cd03760
Location:52 → 247

proteasome_beta_type_4; proteasome beta type-4 subunit. The 20S proteasome, multisubunit proteolytic complex, is the central enzyme of nonlysosomal protein degradation in both the cytosol and nucleus. It is composed of 28 subunits arranged as four homoheptameric rings that ...