Sphk2 sphingosine kinase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Sphk2provided by MGI
Official Full Name: sphingosine kinase 2provided by MGI
Primary source: MGI:MGI:1861380
See related: Ensembl:ENSMUSG00000057342 AllianceGenome:MGI:1861380
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Sk2; Spk2
Summary: This gene encodes a kinase that phosphorylates sphingosine into sphingosine-1-phosphate, which is involved in cell differentiation, motility, and apoptosis. The encoded protein plays a role in maintaining cellular levels of sphingosine-1-phosphate. The gene product also enhances apoptosis in different cell types and suppresses cellular proliferation. In mast cells, the encoded protein is necessary for influx of calcium, protein kinase C activation, and cytokine production and degranulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2010]
Expression: Ubiquitous expression in duodenum adult (RPKM 72.1), adrenal adult (RPKM 42.9) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 7 B3; 7 29.45 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (45358887..45367426, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (45709463..45718002, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deletion of sphingosine kinase 2 attenuates cigarette smoke-mediated chronic obstructive pulmonary disease-like symptoms by reducing lung inflammation.
Title: Deletion of sphingosine kinase 2 attenuates cigarette smoke-mediated chronic obstructive pulmonary disease-like symptoms by reducing lung inflammation.
Sphingosine Kinase 2 in Stromal Fibroblasts Creates a Hospitable Tumor Microenvironment in Breast Cancer.
Title: Sphingosine Kinase 2 in Stromal Fibroblasts Creates a Hospitable Tumor Microenvironment in Breast Cancer.
Loss of sphingosine kinase 2 protects against cisplatin-induced kidney injury.
Title: Loss of sphingosine kinase 2 protects against cisplatin-induced kidney injury.
Sphk1 and Sphk2 Differentially Regulate Erythropoietin Synthesis in Mouse Renal Interstitial Fibroblast-like Cells.
Title: Sphk1 and Sphk2 Differentially Regulate Erythropoietin Synthesis in Mouse Renal Interstitial Fibroblast-like Cells.
Ventricular SK2 upregulation following angiotensin II challenge: Modulation by p21-activated kinase-1.
Title: Ventricular SK2 upregulation following angiotensin II challenge: Modulation by p21-activated kinase-1.
Sphk2 deletion is involved in structural abnormalities and Th17 response but does not aggravate colon inflammation induced by sub-chronic stress.
Title: Sphk2 deletion is involved in structural abnormalities and Th17 response but does not aggravate colon inflammation induced by sub-chronic stress.
Loss of sphingosine kinase 2 enhances Wilm's tumor suppressor gene 1 and nephrin expression in podocytes and protects from streptozotocin-induced podocytopathy and albuminuria in mice.
Title: Loss of sphingosine kinase 2 enhances Wilm's tumor suppressor gene 1 and nephrin expression in podocytes and protects from streptozotocin-induced podocytopathy and albuminuria in mice.
Validation of highly selective sphingosine kinase 2 inhibitors SLM6031434 and HWG-35D as effective anti-fibrotic treatment options in a mouse model of tubulointerstitial fibrosis.
Title: Validation of highly selective sphingosine kinase 2 inhibitors SLM6031434 and HWG-35D as effective anti-fibrotic treatment options in a mouse model of tubulointerstitial fibrosis.
An Unanticipated Role for Sphingosine Kinase-2 in Bone and in the Anabolic Effect of Parathyroid Hormone.
Title: An Unanticipated Role for Sphingosine Kinase-2 in Bone and in the Anabolic Effect of Parathyroid Hormone.
SPHK2-Generated S1P in CD11b(+) Macrophages Blocks STING to Suppress the Inflammatory Function of Alveolar Macrophages.
Title: SPHK2-Generated S1P in CD11b(+) Macrophages Blocks STING to Suppress the Inflammatory Function of Alveolar Macrophages.

Submit: New GeneRIF Correction See all GeneRIFs (65)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (3) 1 citation
Targeted (7) 1 citation
Endonuclease-mediated (4) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH141779 (e-PCR)
- UniSTS:223772

Sphk2 (e-PCR), detects polymorphism
- UniSTS:496532

C76851 (e-PCR)
- UniSTS:159642

RH129117 (e-PCR)
- UniSTS:212425

Sphk2 (e-PCR), detects polymorphism
- UniSTS:527564

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables D-erythro-sphingosine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables D-erythro-sphingosine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables D-erythro-sphingosine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables D-erythro-sphingosine kinase activity	ISO Inferred from Sequence Orthology more info
enables histone binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sphinganine kinase activity	ISO Inferred from Sequence Orthology more info
enables sphingosine-1-phosphate receptor activity	ISO Inferred from Sequence Orthology more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within blood vessel development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within brain development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to phorbol 13-acetate 12-myristate	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of cell growth	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of calcium ion import	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of cell population proliferation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of ceramide biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of ceramide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cytokine production involved in immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of interleukin-13 production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of interleukin-6 production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mast cell activation involved in immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of mast cell degranulation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein kinase C signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of tumor necrosis factor production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of ATP biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of canonical NF-kappaB signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cytochrome-c oxidase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cytochrome-c oxidase activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of reactive oxygen species biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sphinganine-1-phosphate biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in sphinganine-1-phosphate biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in sphinganine-1-phosphate biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in sphingosine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in sphingosine biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sphingosine metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sphingosine metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription initiation-coupled chromatin remodeling	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
is_active_in intracellular membrane-bounded organelle	IBA Inferred from Biological aspect of Ancestor more info
located_in lysosome	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
part_of nucleosome	ISO Inferred from Sequence Orthology more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: sphingosine kinase 2

NP_001166032.1

EC 2.7.1.91

NP_064395.2

EC 2.7.1.91

NP_975009.1

EC 2.7.1.91

XP_006541075.1

EC 2.7.1.91

XP_006541076.1

EC 2.7.1.91

XP_036009169.1

EC 2.7.1.91

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001172561.1 → NP_001166032.1 sphingosine kinase 2

See identical proteins and their annotated locations for NP_001166032.1

Status: REVIEWED

Description

Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AC167242

Consensus CDS

CCDS21261.1

UniProtKB/Swiss-Prot

Q91VA9, Q9DBH6, Q9JIA7

UniProtKB/TrEMBL

Q3U912, Q58E38

Related

ENSMUSP00000147391.2, ENSMUST00000210060.2

Conserved Domains (1) summary

PLN02958
Location:142 → 608

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
NM_020011.5 → NP_064395.2 sphingosine kinase 2

See identical proteins and their annotated locations for NP_064395.2

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein.

Source sequence(s)

AC167242, AK004951

Consensus CDS

CCDS21261.1

UniProtKB/Swiss-Prot

Q91VA9, Q9DBH6, Q9JIA7

UniProtKB/TrEMBL

Q3U912, Q58E38

Related

ENSMUSP00000072615.5, ENSMUST00000072836.7

Conserved Domains (1) summary

PLN02958
Location:142 → 608

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
NM_203280.3 → NP_975009.1 sphingosine kinase 2

See identical proteins and their annotated locations for NP_975009.1

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the same protein as variants 2 and 3.

Source sequence(s)

AC167242, AK151990, BY256088

Consensus CDS

CCDS21261.1

UniProtKB/Swiss-Prot

Q91VA9, Q9DBH6, Q9JIA7

UniProtKB/TrEMBL

Q3U912, Q58E38

Related

ENSMUSP00000103366.3, ENSMUST00000107737.11

Conserved Domains (1) summary

PLN02958
Location:142 → 608

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

45358887..45367426 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006541013.3 → XP_006541076.1 sphingosine kinase 2 isoform X1

See identical proteins and their annotated locations for XP_006541076.1

UniProtKB/Swiss-Prot

Q91VA9, Q9DBH6, Q9JIA7

UniProtKB/TrEMBL

Q3U912, Q58E38

Conserved Domains (1) summary

PLN02958
Location:142 → 608

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
XM_006541012.5 → XP_006541075.1 sphingosine kinase 2 isoform X1

See identical proteins and their annotated locations for XP_006541075.1

UniProtKB/Swiss-Prot

Q91VA9, Q9DBH6, Q9JIA7

UniProtKB/TrEMBL

Q3U912, Q58E38

Conserved Domains (1) summary

PLN02958
Location:142 → 608

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase
XM_036153276.1 → XP_036009169.1 sphingosine kinase 2 isoform X1

UniProtKB/Swiss-Prot

Q91VA9, Q9DBH6, Q9JIA7

UniProtKB/TrEMBL

Q3U912, Q58E38

Conserved Domains (1) summary

PLN02958
Location:142 → 608

PLN02958; diacylglycerol kinase/D-erythro-sphingosine kinase