Asah2 N-acylsphingosine amidohydrolase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Asah2provided by MGI
Official Full Name: N-acylsphingosine amidohydrolase 2provided by MGI
Primary source: MGI:MGI:1859310
See related: Ensembl:ENSMUSG00000024887 AllianceGenome:MGI:1859310
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables N-acylsphingosine amidohydrolase activity. Involved in lipid digestion and sphingolipid metabolic process. Located in caveola; extracellular space; and mitochondrion. Is integral component of plasma membrane. Orthologous to several human genes including ASAH2B (N-acylsphingosine amidohydrolase 2B). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in small intestine adult (RPKM 19.0), large intestine adult (RPKM 15.0) and 18 other tissues See more
Orthologs: human all
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Genomic context

Location:: 19 C1; 19 26.58 cM

Exon count:: 24

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	19	NC_000085.7 (31962046..32080540, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	19	NC_000085.6 (31984646..32103140, complement)

Chromosome 19 - NC_000085.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Neutral ceramidase regulates breast cancer progression by metabolic programming of TREM2-associated macrophages.
Title: Neutral ceramidase regulates breast cancer progression by metabolic programming of TREM2-associated macrophages.
Neutral ceramidase-dependent regulation of macrophage metabolism directs intestinal immune homeostasis and controls enteric infection.
Title: Neutral ceramidase-dependent regulation of macrophage metabolism directs intestinal immune homeostasis and controls enteric infection.
Asah2 Represses the p53-Hmox1 Axis to Protect Myeloid-Derived Suppressor Cells from Ferroptosis.
Title: Asah2 Represses the p53-Hmox1 Axis to Protect Myeloid-Derived Suppressor Cells from Ferroptosis.
These results suggest that neutral ceramidase is a mediator of necroptosis and might be a novel therapeutic target for protection from ischaemic injury.
Title: Loss of neutral ceramidase protects cells from nutrient- and energy -deprivation-induced cell death.
Knocking down NCDase reduced sphingosine accumulation in mitochondria and preserved COX activity after the brain injury.
Title: Essential roles of neutral ceramidase and sphingosine in mitochondrial dysfunction due to traumatic brain injury.
Neutral ceramidase deficiency increases inflammation in a mouse model of inflammatory bowel disease.
Title: Loss of neutral ceramidase increases inflammation in a mouse model of inflammatory bowel disease.
Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA.
Title: Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA.
the predicted HNF-4alpha binding site identified in the Asah2 promoter is functional
Title: Identification of a functional hepatocyte nuclear factor 4 binding site in the neutral ceramidase promoter.
This enzyme is decreased in the small intestine in CFTR transgenic mice with a deletion in CFTR>
Title: Expression of intestinal and lung alkaline sphingomyelinase and neutral ceramidase in cystic fibrosis f508del transgenic mice.
Neutral ceramidase secreted by endothelial cells is released in part associated with caveolin-1.
Title: Neutral ceramidase secreted by endothelial cells is released in part associated with caveolin-1.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (2)

General gene information

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Markers

AI585898 (e-PCR)
- UniSTS:166146

ASAH2 (e-PCR)
- UniSTS:509968

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables N-acylsphingosine amidohydrolase activity	IBA Inferred from Biological aspect of Ancestor more info
enables N-acylsphingosine amidohydrolase activity	IDA Inferred from Direct Assay more info	PubMed
enables N-acylsphingosine amidohydrolase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables N-acylsphingosine amidohydrolase activity	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables ceramidase activity	IEA Inferred from Electronic Annotation more info
enables dihydroceramidase activity	ISO Inferred from Sequence Orthology more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phytoceramidase activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables zinc ion binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in ceramide biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ceramide biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in ceramide catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ceramide catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ceramide catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ceramide catabolic process	ISO Inferred from Sequence Orthology more info
involved_in lipid digestion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in long-chain fatty acid biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to organic substance	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sphingolipid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in sphingosine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in sphingosine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in sphingosine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sphingosine biosynthetic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info
located_in caveola	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular exosome	ISO Inferred from Sequence Orthology more info
is_active_in extracellular region	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with membrane raft	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: neutral ceramidase

Names: N-CDase; NCDase; acylsphingosine deacylase 2; neutral/alkaline ceramidase

NP_061300.1

EC 3.5.1.23

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_018830.1 → NP_061300.1 neutral ceramidase

See identical proteins and their annotated locations for NP_061300.1

Status: PROVISIONAL

Source sequence(s)

AB037181

Consensus CDS

CCDS29749.1

UniProtKB/Swiss-Prot

Q3UWP9, Q8BNP0, Q8BQN7, Q8R236, Q9JHE3

UniProtKB/TrEMBL

B7ZN55, B9EHG7

Related

ENSMUSP00000157744.2, ENSMUST00000236504.2

Conserved Domains (3) summary

PTZ00487
Location:76 → 754

PTZ00487; ceramidase; Provisional

pfam04734
Location:78 → 584

Ceramidase_alk; Neutral/alkaline non-lysosomal ceramidase, N-terminal

pfam17048
Location:586 → 753

Ceramidse_alk_C; Neutral/alkaline non-lysosomal ceramidase, C-terminal