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CIAO2B cytosolic iron-sulfur assembly component 2B [ Homo sapiens (human) ]

Gene ID: 51647, updated on 11-Apr-2024

Summary

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Official Symbol
CIAO2Bprovided by HGNC
Official Full Name
cytosolic iron-sulfur assembly component 2Bprovided by HGNC
Primary source
HGNC:HGNC:24261
See related
Ensembl:ENSG00000166595 MIM:614778; AllianceGenome:HGNC:24261
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
CIA2B; MIP18; FAM96B; CGI-128
Summary
Involved in chromosome segregation; iron-sulfur cluster assembly; and protein maturation by iron-sulfur cluster transfer. Located in cytosol; nucleoplasm; and spindle. Part of CIA complex and MMXD complex. [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in kidney (RPKM 31.6), heart (RPKM 26.9) and 25 other tissues See more
Orthologs
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Genomic context

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Location:
16q22.1
Exon count:
4
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 16 NC_000016.10 (66932065..66934402, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 16 NC_060940.1 (72726365..72728702, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 16 NC_000016.9 (66965968..66968305, complement)

Chromosome 16 - NC_000016.10Genomic Context describing neighboring genes Neighboring gene H3K4me1 hESC enhancer GRCh37_chr16:66945849-66946350 Neighboring gene cadherin 16 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr16:66955545-66956068 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr16:66956069-66956592 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 7571 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 7572 Neighboring gene RRAD, Ras related glycolysis inhibitor and calcium channel regulator Neighboring gene P300/CBP strongly-dependent group 1 enhancer GRCh37_chr16:66961058-66962257 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 10945 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 10946 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr16:66969029-66969641 Neighboring gene carboxylesterase 2 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr16:66974461-66974960 Neighboring gene MED14-independent group 3 enhancer GRCh37_chr16:66975020-66976219 Neighboring gene uncharacterized LOC107984881 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 7573 Neighboring gene MPRA-validated peak2620 silencer Neighboring gene MPRA-validated peak2621 silencer

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Fam96b recruits brain-type creatine kinase to fuel mitotic spindle formation. Zhang XH, et al. Biochim Biophys Acta Mol Cell Res, 2023 Feb. PMID 36503010
  2. FAM96B inhibits the senescence of dental pulp stem cells. Liang H, et al. Cell Biol Int, 2020 May. PMID 32039527
  3. Identification of FAM96B as a novel selenoprotein W binding partner in the brain. Chen P, et al. Biochem Biophys Res Commun, 2019 Apr 23. PMID 30876693
  4. Identification of FAM96B as a novel prelamin A binding partner. Xiong XD, et al. Biochem Biophys Res Commun, 2013 Oct 11. PMID 24041693
  5. Interference of E2-2-mediated effect in endothelial cells by FAM96B through its limited expression of E2-2. Yang W, et al. Cancer Sci, 2011 Oct. PMID 21722264

See all (69) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Fam96b recruits brain-type creatine kinase to fuel mitotic spindle formation.
    Title: Fam96b recruits brain-type creatine kinase to fuel mitotic spindle formation.
  2. FAM96B inhibits the senescence of dental pulp stem cells.
    Title: FAM96B inhibits the senescence of dental pulp stem cells.
  3. Structural insights into Fe-S protein biogenesis by the CIA targeting complex.
    Title: Structural insights into Fe-S protein biogenesis by the CIA targeting complex.
  4. We performed a yeast two-hybrid screen on a human fetal brain cDNA library and identified FAM96B as a novel binding partner of SelW. FRET analyses confirmed the interaction between SelW' and FAM96B.
    Title: Identification of FAM96B as a novel selenoprotein W binding partner in the brain.
  5. Data suggest that CIA2B and MMS19 physically interact with C-terminus of viperin/RSAD2; CIAO1 appears to function as primary viperin-interacting protein; CIA2A binds to N-terminus of viperin in CIAO1-, CIA2B-, and MMS19-independent fashion. (CIA2B = metallochaperone CIA2B/FAM96B; MMS19 = transcription factor MMS19; CIAO1 = cytosolic iron-sulfur assembly component 1; CIA2A = metallochaperone CIA2A/Fam96a)
    Title: Cellular requirements for iron-sulfur cluster insertion into the antiviral radical SAM protein viperin.
  6. CIA2B-CIA1-MMS19 and CIA2A-CIA1 assist different branches of Fe/S protein assembly and intimately link this process to cellular iron regulation via IRP1 Fe/S cluster maturation and IRP2 stabilization.
    Title: Human CIA2A-FAM96A and CIA2B-FAM96B integrate iron homeostasis and maturation of different subsets of cytosolic-nuclear iron-sulfur proteins.
  7. Co-localization experiments by fluorescent confocal microscopy revealed that FAM96B colocalized with prelamin A in HEK-293 cells.
    Title: Identification of FAM96B as a novel prelamin A binding partner.
  8. The mammalian proteins MMS19, MIP18, and ANT2 are involved in cytoplasmic iron-sulfur cluster protein assembly.
    Title: The mammalian proteins MMS19, MIP18, and ANT2 are involved in cytoplasmic iron-sulfur cluster protein assembly.
  9. these findings suggest that FAM96B acts as a regulator of E2-2 through the control of its protein expression.
    Title: Interference of E2-2-mediated effect in endothelial cells by FAM96B through its limited expression of E2-2.
Submit: New GeneRIF Correction

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Replication interactions

Interaction Pubs
HIV-1 NL4-3 replication requires FAM96B as replication is inhibited when FAM96B is deleted through CRISPR/Cas9 genome editing PubMed

Protein interactions

Protein Gene Interaction Pubs
integrase gag-pol HIV-1 Tat is identified to have a physical interaction with family with sequence similarity 96, member B (FAM96B) in human HEK293 and/or Jurkat cell lines by using affinity tagging and purification mass spectrometry analyses PubMed

Go to the HIV-1, Human Interaction Database

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in chromosome segregation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in iron-sulfur cluster assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein maturation by [4Fe-4S] cluster transfer IEA
Inferred from Electronic Annotation
more info
  
involved_in protein maturation by iron-sulfur cluster transfer IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein maturation by iron-sulfur cluster transfer IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
part_of CIA complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of CIA complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of MMXD complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IDA
Inferred from Direct Assay
more info
  
located_in midbody IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in spindle IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
cytosolic iron-sulfur assembly component 2B
Names
MSS19-interacting protein of 18 kDa
family with sequence similarity 96 member B
mitotic spindle-associated MMXD complex subunit MIP18

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_016062.4 → NP_057146.1  cytosolic iron-sulfur assembly component 2B

    See identical proteins and their annotated locations for NP_057146.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the shortest transcript but encodes the supported protein.
    Source sequence(s)
    AF151886, DA270099
    Consensus CDS
    CCDS45506.1
    UniProtKB/Swiss-Prot
    Q9Y3D0
    Related
    ENSP00000387471.2, ENST00000422424.7
    Conserved Domains (1) summary
    cl00941
    Location:23 → 161
    FeS_assembly_P; Iron-sulfur cluster assembly protein

RNA

  1. NR_024525.3 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) contains an additional segment in the 5' region, compared to variant 1. This variant is represented as non-coding because use of the expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AF151886, DA270099, DR155511
    Related
    ENST00000563490.1

  2. NR_046109.2 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) uses alternate splice sites in the 5' region, compared to variant 1. This variant is represented as non-coding because use of the expected translational start codon, as used in variant 1, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).
    Source sequence(s)
    AF151886, BI603322, DA270099
    Related
    ENST00000562362.5

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000016.10 Reference GRCh38.p14 Primary Assembly

    Range
    66932065..66934402 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060940.1 Alternate T2T-CHM13v2.0

    Range
    72726365..72728702 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9Y3D0.1 GenPept UniProtKB/Swiss-Prot:Q9Y3D0

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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