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PolA1 DNA polymerase alpha subunit 1 [ Drosophila melanogaster (fruit fly) ]

Gene ID: 42553, updated on 27-Mar-2024

Summary

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Official Symbol
PolA1provided by FlyBase
Official Full Name
DNA polymerase alpha subunit 1provided by FlyBase
Primary source
FLYBASE:FBgn0259113
Locus tag
Dmel_CG6349
See related
AllianceGenome:FB:FBgn0259113
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
183 kDa pol alpha; AAB24152; CG6349; Ctf4-polalpha; Dmel\CG6349; Dmpol alpha; DmPolalpha; DNA pol alpha; DNA Pol-alpha180; DNA polalpha; DNA Polalpha-180; DNA polymerase alpha 180 kD; DNA-Polalpha; DNApol; DNApol-alpha; DNApol-alpha180; DNApola; DNApolalpha; DNApolalpha180; dpolalpha; dpolalphap180; E(mus304); Pol; pol alpha; Pol alpha; Pol-alpha180; POLA; polalpha; Polalpha; Polalpha 180KDa; POlalpha 180kDa; Polalpha180; polalphap180
Summary
Enables 3'-5'-exodeoxyribonuclease activity and DNA-directed DNA polymerase activity. Involved in DNA replication proofreading. Located in nucleus. Part of alpha DNA polymerase:primase complex. Is expressed in follicle cell; nurse cell; organism; and ovary. Human ortholog(s) of this gene implicated in Van Esch-O'Driscoll syndrome and X-linked reticulate pigmentary disorder. Orthologous to human POLA1 (DNA polymerase alpha 1, catalytic subunit). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:
93F2-93F2; 3-73 cM
Exon count:
6
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3R NT_033777.3 (21666562..21671750, complement)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3R NT_033777.2 (17492284..17497472, complement)

Chromosome 3R - NT_033777.3Genomic Context describing neighboring genes Neighboring gene E2F transcription factor 1 Neighboring gene long non-coding RNA:CR46057 Neighboring gene archease Neighboring gene Juvenile hormone binding protein 16 Neighboring gene uncharacterized protein Neighboring gene long non-coding RNA:CR44096

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Identification of the specific labile sites in the 180 kDa catalytic polypeptide of the Drosophila melanogaster DNA polymerase alpha-primase complex. Kuroda K, et al. J Biochem, 1993 Feb. PMID 8468316
  2. A DNA polymerase alpha-associated 56 kDa protein kinase. Peck VM, et al. Biochem Biophys Res Commun, 1993 Jan 29. PMID 8427578
  3. Mapping an initiation region of DNA replication at a single-copy chromosomal locus in Drosophila melanogaster cells by two-dimensional gel methods and PCR-mediated nascent-strand analysis: multiple replication origins in a broad zone. Shinomiya T, et al. Mol Cell Biol, 1994 Nov. PMID 7935453, Free PMC Article
  4. Immunological comparison of purified DNA polymerase alpha from embryos of Drosophila melanogaster with forms of the enzyme present in vivo. Sauer B, et al. J Biol Chem, 1982 Oct 25. PMID 6811594
  5. Purification and properties of an accessory protein for DNA polymerase alpha/primase. Goulian M, et al. J Biol Chem, 1990 Aug 5. PMID 2165497

See all (120) citations in PubMed

GeneRIFs: Gene References Into Functions

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables 3'-5'-DNA exonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA exonuclease activity, producing 5'-phosphomonoesters IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA replication origin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA-directed DNA polymerase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA-directed DNA polymerase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA-directed DNA polymerase activity IEA
Inferred from Electronic Annotation
more info
  
enables chromatin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables single-stranded DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication proofreading IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA-templated DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in lagging strand elongation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in leading strand elongation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mitotic DNA replication initiation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in mitotic DNA replication initiation IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of alpha DNA polymerase:primase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of alpha DNA polymerase:primase complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
DNA polymerase alpha subunit 1
Names
180 kDa subunit
180 kDa subunit of DNA polymerase alpha
180 kDa subunit of polymerase-primase
182-kDa polymerase subunit
CG6349-PA
DNA pol 180
DNA polymerase 180K
DNA polymerase alpha
DNA polymerase alpha 148,000 alpha subunit
DNA polymerase alpha 180 kD
DNA polymerase alpha 180-kDa subunit
DNA polymerase alpha 180kD
DNA polymerase alpha 180kD subunit
DNA polymerase alpha 182,000 alpha subunit
DNA polymerase-alpha p180 subunit
DNA polymerase-primase 180 kda subunit
DNA-polymerase alpha
DNApol-alpha180
PolA1-PA
enhancer of mus304
NP_536736.2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_033777.3 Reference assembly

    Range
    21666562..21671750 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_080488.3 → NP_536736.2  DNA polymerase alpha subunit 1 [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_536736.2

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    O77034, P26019, Q8T992, Q9VD90
    Related
    FBpp0083514
    Conserved Domains (5) summary
    cd05776
    Location:567 → 789
    DNA_polB_alpha_exo; inactive DEDDy 3'-5' exonuclease domain of eukaryotic DNA polymerase alpha, a family-B DNA polymerase
    cd05532
    Location:852 → 1254
    POLBc_alpha; DNA polymerase type-B alpha subfamily catalytic domain. Three DNA-dependent DNA polymerases type B (alpha, delta, and epsilon) have been identified as essential for nuclear DNA replication in eukaryotes. DNA polymerase (Pol) alpha is almost exclusively ...
    TIGR00592
    Location:52 → 1237
    pol2; DNA polymerase (pol2)
    pfam08996
    Location:1286 → 1459
    zf-DNA_Pol; DNA Polymerase alpha zinc finger
    pfam12254
    Location:24 → 83
    DNA_pol_alpha_N; DNA polymerase alpha subunit p180 N terminal
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P26019.2 P26019

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