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braf B-Raf proto-oncogene, serine/threonine kinase [ Danio rerio (zebrafish) ]

Gene ID: 403065, updated on 11-Apr-2024

Summary

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Official Symbol
brafprovided by ZNC
Official Full Name
B-Raf proto-oncogene, serine/threonine kinaseprovided by ZNC
Primary source
ZFIN:ZDB-GENE-040805-1
See related
Ensembl:ENSDARG00000017661 AllianceGenome:ZFIN:ZDB-GENE-040805-1
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Danio rerio
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as
sierrabraf
Summary
Predicted to enable MAP kinase kinase kinase activity. Predicted to be involved in MAPK cascade. Predicted to act upstream of or within intracellular signal transduction and protein phosphorylation. Predicted to be located in membrane. Predicted to be integral component of membrane. Predicted to be active in plasma membrane. Human ortholog(s) of this gene implicated in several diseases, including Noonan syndrome 7; carcinoma (multiple); cardiofaciocutaneous syndrome (multiple); central nervous system benign neoplasm (multiple); and melanoma (multiple). Orthologous to human BRAF (B-Raf proto-oncogene, serine/threonine kinase). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs
human mouse all
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Genomic context

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Location:
chromosome: 4
Exon count:
20
Annotation release Status Assembly Chr Location
106 current GRCz11 (GCF_000002035.6) 4 NC_007115.7 (12072421..12102123, complement)
105 previous assembly GRCz10 (GCF_000002035.5) 4 NC_007115.6 (12073572..12103187, complement)

Chromosome 4 - NC_007115.7Genomic Context describing neighboring genes Neighboring gene si:dkey-222f8.3 Neighboring gene uncharacterized LOC101883576 Neighboring gene troponin T2c, cardiac Neighboring gene uncharacterized LOC108183387 Neighboring gene mitochondrial ribosomal protein S33 Neighboring gene transmembrane protein 178Bb

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
  • Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
  • BioProject: PRJEB1986
  • Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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Related articles in PubMed

  1. BRAF mutations are sufficient to promote nevi formation and cooperate with p53 in the genesis of melanoma. Patton EE, et al. Curr Biol, 2005 Feb 8. PMID 15694309
  2. Synergistic melanoma cell death mediated by inhibition of both MCL1 and BCL2 in high-risk tumors driven by NF1/PTEN loss. He S, et al. Oncogene, 2021 Sep. PMID 34331013, Free PMC Article
  3. B-raf alternative splicing is dispensable for development but required for learning and memory associated with the hippocampus in the adult mouse. Valluet A, et al. PLoS One, 2010 Dec 22. PMID 21203559, Free PMC Article
  4. Angiogenic efficacy of simplified 2-herb formula (NF3) in zebrafish embryos in vivo and rat aortic ring in vitro. Tse HY, et al. J Ethnopharmacol, 2012 Jan 31. PMID 22138660
  5. Oncogenic BRAF disrupts thyroid morphogenesis and function via twist expression. Anelli V, et al. Elife, 2017 Mar 28. PMID 28350298, Free PMC Article

See all (18) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Gene expression studies nominated TWIST2 as a key effector downstream of BRAF.
    Title: Oncogenic BRAF disrupts thyroid morphogenesis and function via twist expression.
  2. BRAF alternative splicing is differentially regulated in vertebrates. Exon 9b is present in all vertebrates, including Danio rerio, but exon 8b is present only in eutherians.
    Title: B-raf alternative splicing is dispensable for development but required for learning and memory associated with the hippocampus in the adult mouse.
  3. BRAF activation is sufficient for f-nevus formation, and is among the primary events in melanoma development.
    Title: BRAF mutations are sufficient to promote nevi formation and cooperate with p53 in the genesis of melanoma.
Submit: New GeneRIF Correction

Pathways from PubChem

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General gene information

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Homology

Gene Ontology Provided by ZFIN

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables MAP kinase kinase kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in MAPK cascade IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in animal organ development IEA
Inferred from Electronic Annotation
more info
  
involved_in cell development IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within signal transduction IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
serine/threonine-protein kinase B-raf
Names
serine/threonine protein kinase BRAF
v-raf murine sarcoma viral oncogene homolog B1
NP_001311445.1
NP_991307.3
XP_021330649.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001324516.1 → NP_001311445.1  serine/threonine-protein kinase B-raf isoform 1

    Status: VALIDATED

    Source sequence(s)
    BX005210
    UniProtKB/TrEMBL
    A0A8M1P7T0, Q1LYG2
    Related
    ENSDARP00000048390.8, ENSDART00000048391.10
    Conserved Domains (4) summary
    cd01816
    Location:166 → 237
    Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases
    cd14062
    Location:513 → 765
    STKc_Raf; Catalytic domain of the Serine/Threonine Kinases, Raf (Rapidly Accelerated Fibrosarcoma) kinases
    pfam00130
    Location:245 → 290
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    pfam07714
    Location:507 → 764
    Pkinase_Tyr; Protein tyrosine kinase

  2. NM_205744.4 → NP_991307.3  serine/threonine-protein kinase B-raf isoform 2

    Status: VALIDATED

    Source sequence(s)
    BC162198, BX005210
    UniProtKB/TrEMBL
    A0A8M1PHA9, B3DFX5
    Related
    ENSDARP00000016253.10, ENSDART00000023894.11
    Conserved Domains (4) summary
    cd01816
    Location:166 → 237
    Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases
    cd14151
    Location:458 → 731
    STKc_B-Raf; Catalytic domain of the Serine/Threonine Kinase, B-Raf (Rapidly Accelerated Fibrosarcoma) kinase
    pfam00130
    Location:245 → 290
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    pfam07714
    Location:467 → 724
    Pkinase_Tyr; Protein tyrosine kinase

RefSeqs of Annotated Genomes: Danio rerio Annotation Release 106 details...Open this link in a new tab

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCz11 Primary Assembly

Genomic

  1. NC_007115.7 Reference GRCz11 Primary Assembly

    Range
    12072421..12102123 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_021474974.1 → XP_021330649.1  serine/threonine-protein kinase B-raf isoform X1

    UniProtKB/TrEMBL
    A0A8M9Q0C0
    Related
    ENSDART00000132650.2
    Conserved Domains (3) summary
    cd01816
    Location:166 → 237
    Raf_RBD; Ubiquitin domain of Raf serine/threonine kinases
    pfam00130
    Location:245 → 290
    C1_1; Phorbol esters/diacylglycerol binding domain (C1 domain)
    cl21453
    Location:458 → 575
    PKc_like; Protein Kinases, catalytic domain
Nucleotide Protein Strain
Heading Accession and Version

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