ilk integrin-linked kinase [Danio rerio (zebrafish)] - Gene

Summary

Official Symbol: ilkprovided by ZNC
Official Full Name: integrin-linked kinaseprovided by ZNC
Primary source: ZFIN:ZDB-GENE-040426-1435
See related: Ensembl:ENSDARG00000056964 AllianceGenome:ZFIN:ZDB-GENE-040426-1435
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Danio rerio
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes; Danionidae; Danioninae; Danio
Also known as: zgc:65842
Summary: Enables protein kinase activity. Acts upstream of or within several processes, including circulatory system development; muscle attachment; and tendon development. Located in Z disc and sarcolemma. Is expressed in brain; cardiovascular system; muscle; myoblast; and somite. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to human ILK (integrin linked kinase). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: human mouse all
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Genomic context

Location:: chromosome: 10

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
106	current	GRCz11 (GCF_000002035.6)	10	NC_007121.7 (24725259..24759539, complement)
105	previous assembly	GRCz10 (GCF_000002035.5)	10	NC_007121.6 (24755807..24790087, complement)

Chromosome 10 - NC_007121.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
Description: Sequencing the Zebrafish transcriptome from a range of tissues and developmental stages
BioProject: PRJEB1986
Analysis date: Fri Dec 8 19:48:10 2017

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Using the ILK deficient zebrafish heart failure mutant main squeeze (msq), which shows reduced PKB phosphorylation and thereby impaired cardiac contractile force, we identified here, in an automated small compound screen, the protein phosphatase inhibitors calyculin A and okadaic acid significantly restoring myocardial contractile function by reconstituting PKB phosphorylation in msq ILK-deficient zebrafish embryos.
Title: Therapeutic Chemical Screen Identifies Phosphatase Inhibitors to Reconstitute PKB Phosphorylation and Cardiac Contractility in ILK-Deficient Zebrafish.
Data show that integrin-linked kinase is an essential component downstream of laminin and integrin alpha7, providing strengthening of skeletal muscle fibre adhesion with the extracellular matrix.
Title: Zebrafish integrin-linked kinase is required in skeletal muscles for strengthening the integrin-ECM adhesion complex.
The lost-contact mutant on chromosome 10 encodes a nonsense mutation (Y319X) associated with defective cardiomyocytes & endothelial cells that leads to severe myocardial dysfunction. There is epistatic regulation between laminin-alpha4, integrin, & Ilk.
Title: Laminin-alpha4 and integrin-linked kinase mutations cause human cardiomyopathy via simultaneous defects in cardiomyocytes and endothelial cells.
Heart failure in recessive embryonic lethal zebrafish mutant main squeeze (msq) mutants is due to a mutation in the integrin-linked kinase (ilk) gene.
Title: Integrin-linked kinase, a novel component of the cardiac mechanical stretch sensor, controls contractility in the zebrafish heart.

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General gene information

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Markers

fc69a01.y1 (e-PCR)
- UniSTS:194269

fj86f09.x1 (e-PCR)
- UniSTS:200159

Gene Ontology Provided by ZFIN

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within blood vessel development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cardiac muscle cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within cardiac ventricle formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell-matrix adhesion	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cell-matrix adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endothelial cell development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within endothelial cell development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart contraction	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within heart contraction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within heart development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in integrin-mediated signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within integrin-mediated signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within muscle attachment	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within muscle attachment	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within protein phosphorylation	IC Inferred by Curator more info	PubMed
acts_upstream_of_or_within regulation of heart contraction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in substrate adhesion-dependent cell spreading	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within tendon development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within tendon development	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in Z disc	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in focal adhesion	IBA Inferred from Biological aspect of Ancestor more info
located_in lamellipodium	IEA Inferred from Electronic Annotation more info
located_in sarcolemma	IDA Inferred from Direct Assay more info	PubMed
colocalizes_with stress fiber	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: integrin-linked protein kinase

Names: loc; lost-contact; main squeeze; msq; zilk

NP_956865.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_200571.1 → NP_956865.1 integrin-linked protein kinase

See identical proteins and their annotated locations for NP_956865.1

Status: PROVISIONAL

Source sequence(s)

BC056593

UniProtKB/TrEMBL

A0A8M1PBF3, Q6PHD6

Related

ENSDARP00000073983.2, ENSDART00000079528.3

Conserved Domains (5) summary

cd00204
Location:28 → 140

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd14057
Location:197 → 449

PK_ILK; Pseudokinase domain of Integrin Linked Kinase

pfam07714
Location:193 → 446

Pkinase_Tyr; Protein tyrosine kinase

pfam12796
Location:38 → 130

Ank_2; Ankyrin repeats (3 copies)

sd00045
Location:33 → 64

ANK; ANK repeat [structural motif]