Spi1 Spi-1 proto-oncogene [Rattus norvegicus (Norway rat)] - Gene

Summary

Symbol: Spi1provided by RGD
Full Name: Spi-1 proto-oncogeneprovided by RGD
Primary source: RGD:1359607
See related: Ensembl:ENSRNOG00000012172 AllianceGenome:RGD:1359607
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Pu.1; Sfpi1
Summary: Enables DNA-binding transcription factor activity and sequence-specific DNA binding activity. Involved in cellular response to ethanol; negative regulation of transcription, DNA-templated; and positive regulation of transcription, DNA-templated. Located in nucleus. Used to study alcohol use disorder. Biomarker of alcohol use disorder. Orthologous to human SPI1 (Spi-1 proto-oncogene). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Spleen (RPKM 127.3), Lung (RPKM 63.9) and 8 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 3q24

Exon count:: 5

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (97529509..97548204)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (77059744..77093730)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (79918127..79937708)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PU.1 interaction with p50 promotes microglial-mediated inflammation in secondary spinal cord injury in SCI rats.
Title: PU.1 interaction with p50 promotes microglial-mediated inflammation in secondary spinal cord injury in SCI rats.
Salmonella pathogenicity island 1 knockdown confers protection against myocardial fibrosis and inflammation in uremic cardiomyopathy via down-regulation of S100 Calcium Binding Protein A8/A9 transcription.
Title: Salmonella pathogenicity island 1 knockdown confers protection against myocardial fibrosis and inflammation in uremic cardiomyopathy via down-regulation of S100 Calcium Binding Protein A8/A9 transcription.
Four upregulated transcription factors Pax6, Tp73, Cebpd, and Spi1, appeared as potent regulators of chronic post-traumatic brain injury (TBI) gene expression. They regulate molecular networks contributing to post-injury secondary damage, including apoptosis and inflammation, strengthening the feasibility of therapeutically targeting these molecular networks even after the acute post-TBI period.
Title: Transcription factors Tp73, Cebpd, Pax6, and Spi1 rather than DNA methylation regulate chronic transcriptomics changes after experimental traumatic brain injury.
Nrf2 regulates the expression and activity of PU.1 and that antioxidant response and immune activation are coordinately regulated within the AM.
Title: Nrf2 regulates PU.1 expression and activity in the alveolar macrophage.
PU.1-silenced semi-mature dendritic cells induced partial immune tolerance both in vitro and in vivo, which could be used as a new strategy to promote intestinal transplantation tolerance.
Title: PU.1-silenced dendritic cells prolong allograft survival in rats receiving intestinal transplantation.
interaction of mutants with IRF-8
Title: Analysis of PU.1/ICSBP (IRF-8) complex formation with various PU.1 mutants: molecular cloning of rat Icsbp (Irf-8) cDNA.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC186377P2 (e-PCR)
- UniSTS:271703

RH135185 (e-PCR)
- UniSTS:218462

Sfpi1 (e-PCR), detects polymorphism
- UniSTS:547306

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables DNA binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription repressor activity	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription repressor activity	ISO Inferred from Sequence Orthology more info
enables DNA-binding transcription repressor activity, RNA polymerase II-specific	ISO Inferred from Sequence Orthology more info
enables NFAT protein binding	ISO Inferred from Sequence Orthology more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables RNA polymerase II-specific DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables STAT family protein binding	IEA Inferred from Electronic Annotation more info
enables STAT family protein binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables cis-regulatory region sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables histone deacetylase binding	ISO Inferred from Sequence Orthology more info
enables molecular adaptor activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein sequestering activity	ISO Inferred from Sequence Orthology more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables sequence-specific DNA binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	ISO Inferred from Sequence Orthology more info
enables transcription cis-regulatory region binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in TRAIL-activated apoptotic signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_positive_effect TRAIL-activated apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within anatomical structure regression	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within apoptotic process involved in blood vessel morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to ethanol	IEP Inferred from Expression Pattern more info	PubMed
involved_in defense response to tumor cell	IEA Inferred from Electronic Annotation more info
involved_in defense response to tumor cell	ISO Inferred from Sequence Orthology more info
involved_in endothelial to hematopoietic transition	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within erythrocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in follicular B cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in germinal center B cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within granulocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in immature B cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in interleukin-6-mediated signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in interleukin-6-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in lipopolysaccharide-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lymphocyte differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within lymphoid progenitor cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within macrophage differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within myeloid dendritic cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in myeloid leukocyte differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within myeloid leukocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in myeloid leukocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of MHC class II biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of adipose tissue development	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neutrophil degranulation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of non-canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of non-canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein localization to chromatin	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in oncogene-induced cell senescence	ISO Inferred from Sequence Orthology more info
involved_in osteoclast differentiation	IEP Inferred from Expression Pattern more info	PubMed
involved_in pericyte cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of B cell differentiation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of B cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of B cell differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of DNA-templated transcription	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of antifungal innate immune response	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of miRNA transcription	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of miRNA transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of microglial cell mediated cytotoxicity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of myeloid dendritic cell chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of p38MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in pro-T cell differentiation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in regulation of erythrocyte differentiation	IEA Inferred from Electronic Annotation more info
involved_in regulation of erythrocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in regulation of myeloid progenitor cell differentiation	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in response to ethanol	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within somatic stem cell population maintenance	ISO Inferred from Sequence Orthology more info
involved_in transcription initiation-coupled chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in transcription initiation-coupled chromatin remodeling	ISO Inferred from Sequence Orthology more info
involved_in transcription initiation-coupled chromatin remodeling	ISS Inferred from Sequence or Structural Similarity more info
involved_in transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within vasculature development	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of chromatin	IEA Inferred from Electronic Annotation more info
part_of chromatin	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
part_of transcription regulator complex	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: transcription factor PU.1

Names: SFFV proviral integration 1; hematopoietic transcription factor PU.1; spleen focus forming virus (SFFV) proviral integration oncogene spi1; spleen focus forming virus proviral integration oncogene spi1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001005892.2 → NP_001005892.1 transcription factor PU.1

See identical proteins and their annotated locations for NP_001005892.1

Status: PROVISIONAL

Source sequence(s)

BC091185

UniProtKB/Swiss-Prot

Q5BK69, Q6BDS1

UniProtKB/TrEMBL

A0A8L2UIY0, A6HNA6

Related

ENSRNOP00000016306.5, ENSRNOT00000016306.7

Conserved Domains (1) summary

pfam00178
Location:172 → 253

Ets; Ets-domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086021.1 Reference GRCr8

Range

97529509..97548204

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006234532.5 → XP_006234594.1 transcription factor PU.1 isoform X1

UniProtKB/TrEMBL

A0A8I5ZJK5, A0A8L2UIY0

Related

ENSRNOP00000077751.1

Conserved Domains (1) summary

pfam00178
Location:173 → 254

Ets; Ets-domain