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ILF2 interleukin enhancer binding factor 2 [ Homo sapiens (human) ]

Gene ID: 3608, updated on 7-Apr-2024

Summary

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Official Symbol
ILF2provided by HGNC
Official Full Name
interleukin enhancer binding factor 2provided by HGNC
Primary source
HGNC:HGNC:6037
See related
Ensembl:ENSG00000143621 MIM:603181; AllianceGenome:HGNC:6037
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Homo sapiens
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as
NF45; PRO3063
Summary
The protein encoded by this gene is a transcription factor required for T-cell expression of the interleukin 2 gene. It also binds RNA and is an essential component for encapsidation and protein priming of hepatitis B viral polymerase. The encoded 45 kDa protein (NF45, ILF2) forms a complex with the 90 kDa interleukin enhancer-binding factor 3 (NF90, ILF3), and this complex has been shown to affect the redistribution of nuclear mRNA to the cytoplasm, to repair DNA breaks by nonhomologous end joining, and to negatively regulate the microRNA processing pathway. Knockdown of NF45 or NF90 protein retards cell growth, possibly by inhibition of mRNA stabilization. Alternative splicing results in multiple transcript variants. Related pseudogenes have been found on chromosomes 3 and 14. [provided by RefSeq, Dec 2014]
Expression
Ubiquitous expression in testis (RPKM 65.5), bone marrow (RPKM 58.7) and 25 other tissues See more
Orthologs
mouse all
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Genomic context

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Location:
1q21.3
Exon count:
14
Annotation release Status Assembly Chr Location
RS_2023_10 current GRCh38.p14 (GCF_000001405.40) 1 NC_000001.11 (153661788..153670993, complement)
RS_2023_10 current T2T-CHM13v2.0 (GCF_009914755.1) 1 NC_060925.1 (152799053..152808258, complement)
105.20220307 previous assembly GRCh37.p13 (GCF_000001405.25) 1 NC_000001.10 (153634264..153643469, complement)

Chromosome 1 - NC_000001.11Genomic Context describing neighboring genes Neighboring gene S100 calcium binding protein A13 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:153601055-153601878 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1750 Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1342 Neighboring gene S100 calcium binding protein A1 Neighboring gene chromatin target of PRMT1 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1751 Neighboring gene SNAP associated protein Neighboring gene ATAC-STARR-seq lymphoblastoid silent region 1343 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1752 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1753 Neighboring gene ATAC-STARR-seq lymphoblastoid active region 1754 Neighboring gene tRNA-iMet (anticodon CAT) 1-1 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:153650401-153651382 Neighboring gene H3K27ac-H3K4me1 hESC enhancer GRCh37_chr1:153651383-153652363 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:153656981-153657482 Neighboring gene H3K4me1 hESC enhancer GRCh37_chr1:153657483-153657982 Neighboring gene CDK7 strongly-dependent group 2 enhancer GRCh37_chr1:153659386-153660585 Neighboring gene natriuretic peptide receptor 1 Neighboring gene microRNA 8083

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: HPA RNA-seq normal tissues
  • Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
  • BioProject: PRJEB4337
  • Publication: PMID 24309898
  • Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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Related articles in PubMed

  1. Interleukin Enhancer Binding Factor 2 Regulates Cell Viability and Apoptosis of Human Brain Vascular Smooth Muscle Cells. Wei L, et al. J Mol Neurosci, 2021 Feb. PMID 32748330
  2. Circular RNA 406961 interacts with ILF2 to regulate PM(2.5)-induced inflammatory responses in human bronchial epithelial cells via activation of STAT3/JNK pathways. Jia Y, et al. Environ Int, 2020 Aug. PMID 32388272
  3. Enterovirus 71 Represses Interleukin Enhancer-Binding Factor 2 Production and Nucleus Translocation to Antagonize ILF2 Antiviral Effects. Jin J, et al. Viruses, 2019 Dec 23. PMID 31878072, Free PMC Article
  4. Cellular Interleukin Enhancer-Binding Factor 2, ILF2, Inhibits Japanese Encephalitis Virus Replication In Vitro. Cui X, et al. Viruses, 2019 Jun 17. PMID 31212927, Free PMC Article
  5. Expression and Clinical Significance of ILF2 in Gastric Cancer. Yin ZH, et al. Dis Markers, 2017. PMID 28831206, Free PMC Article

See all (279) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. NUSAP1 Binds ILF2 to Modulate R-Loop Accumulation and DNA Damage in Prostate Cancer.
    Title: NUSAP1 Binds ILF2 to Modulate R-Loop Accumulation and DNA Damage in Prostate Cancer.
  2. Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2).
    Title: Serine hydroxymethyltransferase 2 (SHMT2) potentiates the aggressive process of oral squamous cell carcinoma by binding to interleukin enhancer-binding factor 2 (ILF2).
  3. ILF2 enhances the DNA cytosine deaminase activity of tumor mutator APOBEC3B in multiple myeloma cells.
    Title: ILF2 enhances the DNA cytosine deaminase activity of tumor mutator APOBEC3B in multiple myeloma cells.
  4. Interleukin Enhancer Binding Factor 2 Regulates Cell Viability and Apoptosis of Human Brain Vascular Smooth Muscle Cells.
    Title: Interleukin Enhancer Binding Factor 2 Regulates Cell Viability and Apoptosis of Human Brain Vascular Smooth Muscle Cells.
  5. CRNDE-h transcript/miR-136-5p axis regulates interleukin enhancer binding factor 2 expression to promote hepatocellular carcinoma cell proliferation.
    Title: CRNDE-h transcript/miR-136-5p axis regulates interleukin enhancer binding factor 2 expression to promote hepatocellular carcinoma cell proliferation.
  6. Deletion of interleukin enhancer binding factor 2 (ILF2) resulted in defective biliary development and bile flow blockage.
    Title: Deletion of interleukin enhancer binding factor 2 (ILF2) resulted in defective biliary development and bile flow blockage.
  7. NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA Decay.
    Title: NF45 and NF90 Regulate Mitotic Gene Expression by Competing with Staufen-Mediated mRNA Decay.
  8. Cereblon Promotes the Ubiquitination and Proteasomal Degradation of Interleukin Enhancer-Binding Factor 2.
    Title: Cereblon Promotes the Ubiquitination and Proteasomal Degradation of Interleukin Enhancer-Binding Factor 2.
  9. Circular RNA 406961 interacts with ILF2 to regulate PM2.5-induced inflammatory responses in human bronchial epithelial cells via activation of STAT3/JNK pathways.
    Title: Circular RNA 406961 interacts with ILF2 to regulate PM(2.5)-induced inflammatory responses in human bronchial epithelial cells via activation of STAT3/JNK pathways.
  10. Enterovirus 71 Represses Interleukin Enhancer-Binding Factor 2 Production and Nucleus Translocation to Antagonize ILF2 Antiviral Effects.
    Title: Enterovirus 71 Represses Interleukin Enhancer-Binding Factor 2 Production and Nucleus Translocation to Antagonize ILF2 Antiviral Effects.
Submit: New GeneRIF Correction See all GeneRIFs (39)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

EBI GWAS Catalog

Description
The CCND1 c.870G>A polymorphism is a risk factor for t(11;14)(q13;q32) multiple myeloma.
EBI GWAS Catalog

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

Genotypes

HIV-1 interactions

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Protein interactions

Protein Gene Interaction Pubs
Envelope surface glycoprotein gp120 env Tandem affinity purification and mass spectrometry analysis identify interleukin enhancer binding factor 2 (ILF2, NF45), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Gag-Pol gag-pol Tandem affinity purification and mass spectrometry analysis identify interleukin enhancer binding factor 2 (ILF2, NF45), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Nef nef Tandem affinity purification and mass spectrometry analysis identify interleukin enhancer binding factor 2 (ILF2, NF45), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Pr55(Gag) gag Tandem affinity purification and mass spectrometry analysis identify interleukin enhancer binding factor 2 (ILF2, NF45), HIV-1 Gag, Gag/Pol, gp120, and Nef incorporated into staufen1 RNP complexes isolated from HIV-1-expressing cells PubMed
Rev rev HIV-1 Rev interacting protein, interleukin enhancer binding factor 2 (ILF2), is identified by the in-vitro binding experiments involving cytosolic or nuclear extracts from HeLa cells. The interaction of Rev with ILF2 is increased by RRE PubMed
Tat tat Interaction of HIV-1 Tat with ILF2 in T-cells is identified by a proteomic strategy based on affinity chromatography coupled with mass spectrometry PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC8391

Gene Ontology Provided by GOA

Function Evidence Code Pubs
enables DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA binding HDA PubMed 
enables double-stranded RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables double-stranded RNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in positive regulation of DNA-templated transcription IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of DNA-templated transcription IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IDA
Inferred from Direct Assay
more info
  
located_in cytosol TAS
Traceable Author Statement
more info
  
located_in extracellular region HDA PubMed 
located_in extracellular region TAS
Traceable Author Statement
more info
  
located_in ficolin-1-rich granule lumen TAS
Traceable Author Statement
more info
  
located_in membrane HDA PubMed 
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
part_of ribonucleoprotein complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in specific granule lumen TAS
Traceable Author Statement
more info
  
located_in tertiary granule lumen TAS
Traceable Author Statement
more info
  

General protein information

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Preferred Names
interleukin enhancer-binding factor 2
Names
interleukin enhancer binding factor 2, 45kD
interleukin enhancer binding factor 2, 45kDa
nuclear factor of activated T-cells, 45-kDa

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001267809.2 → NP_001254738.1  interleukin enhancer-binding factor 2 isoform 2

    See identical proteins and their annotated locations for NP_001254738.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in its 5' UTR and uses an in-frame downstream start codon, compared to variant 1. The encoded isoform (2) is shorter at the N-terminus, compared to isoform 1.
    Source sequence(s)
    AK223326, AK302214, BC000382, BX450311, DC412988
    Consensus CDS
    CCDS72919.1
    UniProtKB/TrEMBL
    B4DY09, F4ZW63, Q53FG3
    Related
    ENSP00000482909.1, ENST00000615950.4
    Conserved Domains (1) summary
    smart00572
    Location:60 → 300
    DZF; domain in DSRM or ZnF_C2H2 domain containing proteins

  2. NM_004515.4 → NP_004506.2  interleukin enhancer-binding factor 2 isoform 1

    See identical proteins and their annotated locations for NP_004506.2

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes the longer isoform (1).
    Source sequence(s)
    AK223326, BC000382, BX450311, DC412988
    Consensus CDS
    CCDS1050.1
    UniProtKB/Swiss-Prot
    A6NDB0, B2R8G7, Q12905, Q5SR10, Q5SR11, Q7L7R3, Q9BWD4, Q9P1N0
    UniProtKB/TrEMBL
    F4ZW62, F4ZW63, Q53FG3
    Related
    ENSP00000355011.4, ENST00000361891.9
    Conserved Domains (1) summary
    smart00572
    Location:98 → 338
    DZF; domain in DSRM or ZnF_C2H2 domain containing proteins

RefSeqs of Annotated Genomes: GCF_000001405.40-RS_2023_10

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCh38.p14 Primary Assembly

Genomic

  1. NC_000001.11 Reference GRCh38.p14 Primary Assembly

    Range
    153661788..153670993 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

Alternate T2T-CHM13v2.0

Genomic

  1. NC_060925.1 Alternate T2T-CHM13v2.0

    Range
    152799053..152808258 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q12905.2 GenPept UniProtKB/Swiss-Prot:Q12905

Gene LinkOut

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