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bru1 bruno 1 [ Drosophila melanogaster (fruit fly) ]

Gene ID: 34648, updated on 10-May-2024

Summary

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Official Symbol
bru1provided by FlyBase
Official Full Name
bruno 1provided by FlyBase
Primary source
FLYBASE:FBgn0000114
Locus tag
Dmel_CG31762
See related
AllianceGenome:FB:FBgn0000114
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
aret; Aret; ARET; aret/bruno1; arrest/Bruno; BcDNA:GM15173; bru; Bru; bru-1; Bru1; bruno; Bruno; CG31762; CG6319; Dm Bru; Dmel\CG31762; ms(2)01284; NEST:bs01g04; NEST:bs34h11
Summary
Enables mRNA 3'-UTR binding activity. Involved in several processes, including female germ-line stem cell population maintenance; positive regulation of exit from mitosis; and regulation of gene expression. Located in P granule; nuclear body; and nuclear membrane. Is expressed in several structures, including embryonic/larval fat body; flight muscle cell; germline cell; gonad; and hindgut. Human ortholog(s) of this gene implicated in developmental and epileptic encephalopathy. Orthologous to human CELF1 (CUGBP Elav-like family member 1) and CELF2 (CUGBP Elav-like family member 2). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs
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Genomic context

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Location:
33D2-33E1; 2-46 cM
Exon count:
20
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 2L NT_033779.5 (12175004..12313438)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 2L NT_033779.4 (12175004..12313438)

Chromosome 2L - NT_033779.5Genomic Context describing neighboring genes Neighboring gene long non-coding RNA:CR43356 Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene atilla Neighboring gene crooked Neighboring gene uncharacterized protein Neighboring gene long non-coding RNA:CR45282 Neighboring gene long non-coding RNA:CR45283

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression. Reveal B, et al. RNA Biol, 2011 Nov-Dec. PMID 21955496, Free PMC Article
  2. Bruno protein contains an expanded RNA recognition motif. Lyon AM, et al. Biochemistry, 2009 Dec 29. PMID 19919093, Free PMC Article
  3. From factors to mechanisms: translation and translational control in eukaryotes. Preiss T, et al. Curr Opin Genet Dev, 1999 Oct. PMID 10508691
  4. Characterization of an invertebrate nicotinic acetylcholine receptor gene: the ard gene of Drosophila melanogaster. Sawruk E, et al. FEBS Lett, 1988 Aug 1. PMID 3136037
  5. Knock down analysis reveals critical phases for specific oskar noncoding RNA functions during Drosophila oogenesis. Kenny A, et al. G3 (Bethesda), 2021 Dec 8. PMID 34586387, Free PMC Article

See all (202) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Results identify a novel regulatory circuit in drosophila, with Bru restricting the activity of osk, and osk in turn restricting the activity of Bru.
    Title: oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction.
  2. Here we describe an in vivo assay system to monitor the spatial pattern of Bru-dependent repression, separate from the full complexity of osk regulation.
    Title: Region-specific activation of oskar mRNA translation by inhibition of Bruno-mediated repression.
  3. Aret potently promotes flight muscle splicing patterns when ectopically expressed in Drosophila jump muscles.
    Title: Arrest is a regulator of fiber-specific alternative splicing in the indirect flight muscles of Drosophila.
  4. A role for Bruno in Enok-controlled GSC maintenance
    Title: The Drosophila putative histone acetyltransferase Enok maintains female germline stem cells through regulating Bruno and the niche.
  5. Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression.(
    Title: Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression.
  6. Bruno RNA recognition motif contains four antiparallel beta-strands and two alpha-helices.
    Title: Bruno protein contains an expanded RNA recognition motif.
  7. Repression by Bruno during oogenesis is required to restrict Gcl expression in the early embryo.
    Title: Bruno negatively regulates germ cell-less expression in a BRE-independent manner.
  8. Bruno shuttles between the nucleus and cytoplasm, and may first bind oskar mRNA in the nucleus.
    Title: Two distinct domains of Bruno bind specifically to the oskar mRNA.
  9. Ectopic expression of bruno prolongs oskar repression and blocks posterior body patterning.
    Title: A late phase of Oskar accumulation is crucial for posterior patterning of the Drosophila embryo, and is blocked by ectopic expression of Bruno.
  10. we show that Bruno binds specifically to the gurken 3'UTR and the dorso-ventral defects caused by Bruno over-expression are due to a reduction of Gurken levels in the oocyte, Bruno plays similar roles in translational regulation of gurken and oskar.
    Title: Bruno regulates gurken during Drosophila oogenesis.
Submit: New GeneRIF Correction See all GeneRIFs (14)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

mobile_element

  • Loc: 12197314-12197348 mobile_element_type = transposon:INE-1{}2179
  • Loc: 12262559-12262626 mobile_element_type = transposon:H{}2183
  • Loc: 12285973-12286003 mobile_element_type = transposon:INE-1{}2181

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables RNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables mRNA 3'-UTR binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables mRNA 3'-UTR binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables mRNA 3'-UTR binding TAS
Traceable Author Statement
more info
 PubMed 
enables mRNA binding ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
enables mRNA binding NAS
Non-traceable Author Statement
more info
 PubMed 
enables mRNA binding TAS
Traceable Author Statement
more info
 PubMed 
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in female germ-line stem cell population maintenance IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in germ cell development IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mRNA splice site recognition IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in negative regulation of oskar mRNA translation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of oskar mRNA translation NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in negative regulation of oskar mRNA translation TAS
Traceable Author Statement
more info
 PubMed 
involved_in negative regulation of translation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in oogenesis HMP PubMed 
involved_in oogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of exit from mitosis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of stem cell differentiation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of alternative mRNA splicing, via spliceosome HMP PubMed 
involved_in regulation of alternative mRNA splicing, via spliceosome IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of alternative mRNA splicing, via spliceosome IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of myofibril size IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in sarcomere organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in P granule IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nuclear body IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nuclear membrane IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IC
Inferred by Curator
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IGI
Inferred from Genetic Interaction
more info
 PubMed 
part_of ribonucleoprotein complex IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
bruno 1
Names
CG31762-PA
CG31762-PB
CG31762-PD
CG31762-PE
CG31762-PF
CG31762-PG
CG31762-PH
CG31762-PI
CG31762-PJ
CG31762-PK
CG31762-PL
arrest
bru1-PA
bru1-PB
bru1-PD
bru1-PE
bru1-PF
bru1-PG
bru1-PH
bru1-PI
bru1-PJ
bru1-PK
bru1-PL
bruno

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_033779.5 Reference assembly

    Range
    12175004..12313438
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001273478.1 → NP_001260407.1  bruno 1, isoform K [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001260407.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    M9PDB0, O02374
    Conserved Domains (4) summary
    cd12631
    Location:119 → 200
    RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
    cd12640
    Location:485 → 563
    RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
    TIGR01661
    Location:122 → 571
    ELAV_HUD_SF; ELAV/HuD family splicing factor
    cl17169
    Location:206 → 287
    RRM_SF; RNA recognition motif (RRM) superfamily

  2. NM_001273477.1 → NP_001260406.1  bruno 1, isoform J [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001260406.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    M9PDB0, O02374
    Conserved Domains (4) summary
    cd12631
    Location:119 → 200
    RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
    cd12640
    Location:485 → 563
    RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
    TIGR01661
    Location:122 → 571
    ELAV_HUD_SF; ELAV/HuD family splicing factor
    cl17169
    Location:206 → 287
    RRM_SF; RNA recognition motif (RRM) superfamily

  3. NM_165002.2 → NP_723737.1  bruno 1, isoform B [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_723737.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    C8VV87, Q8IP90
    Related
    FBpp0079926
    Conserved Domains (4) summary
    cd12631
    Location:356 → 437
    RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
    cd12636
    Location:443 → 524
    RRM2_Bruno_like; RNA recognition motif 2 in Drosophila melanogaster Bruno protein and similar proteins
    cd12640
    Location:722 → 800
    RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
    TIGR01661
    Location:359 → 808
    ELAV_HUD_SF; ELAV/HuD family splicing factor

  4. NM_001414076.1 → NP_001400994.1  bruno 1, isoform L [Drosophila melanogaster]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A9F2H0X2

  5. NM_001273475.1 → NP_001260404.1  bruno 1, isoform H [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001260404.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    M9PDB0, O02374
    Conserved Domains (4) summary
    cd12631
    Location:119 → 200
    RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
    cd12640
    Location:485 → 563
    RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
    TIGR01661
    Location:122 → 571
    ELAV_HUD_SF; ELAV/HuD family splicing factor
    cl17169
    Location:206 → 287
    RRM_SF; RNA recognition motif (RRM) superfamily

  6. NM_001273476.1 → NP_001260405.1  bruno 1, isoform I [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001260405.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    M9PDB0, O02374
    Conserved Domains (4) summary
    cd12631
    Location:119 → 200
    RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
    cd12640
    Location:485 → 563
    RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
    TIGR01661
    Location:122 → 571
    ELAV_HUD_SF; ELAV/HuD family splicing factor
    cl17169
    Location:206 → 287
    RRM_SF; RNA recognition motif (RRM) superfamily

  7. NM_001273474.1 → NP_001260403.1  bruno 1, isoform G [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001260403.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    C8VV87, Q8IP90
    Conserved Domains (4) summary
    cd12631
    Location:356 → 437
    RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
    cd12636
    Location:443 → 524
    RRM2_Bruno_like; RNA recognition motif 2 in Drosophila melanogaster Bruno protein and similar proteins
    cd12640
    Location:722 → 800
    RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
    TIGR01661
    Location:359 → 808
    ELAV_HUD_SF; ELAV/HuD family splicing factor

  8. NM_165003.3 → NP_723738.2  bruno 1, isoform F [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_723738.2

    Status: REVIEWED

    UniProtKB/TrEMBL
    O02374, Q8IP89
    Conserved Domains (4) summary
    cd12631
    Location:150 → 231
    RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
    cd12640
    Location:532 → 610
    RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
    TIGR01661
    Location:153 → 618
    ELAV_HUD_SF; ELAV/HuD family splicing factor
    cl17169
    Location:237 → 318
    RRM_SF; RNA recognition motif (RRM) superfamily

  9. NM_165004.3 → NP_723739.1  bruno 1, isoform A [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_723739.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    O02374, Q960Z4
    Related
    FBpp0079927
    Conserved Domains (4) summary
    cd12631
    Location:150 → 231
    RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
    cd12640
    Location:516 → 594
    RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
    TIGR01661
    Location:153 → 602
    ELAV_HUD_SF; ELAV/HuD family splicing factor
    cl17169
    Location:237 → 318
    RRM_SF; RNA recognition motif (RRM) superfamily

  10. NM_001169492.2 → NP_001162963.1  bruno 1, isoform E [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_001162963.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    O02374, Q960Z4
    Conserved Domains (4) summary
    cd12631
    Location:150 → 231
    RRM1_CELF1_2_Bruno; RNA recognition motif 1 in CUGBP Elav-like family member CELF-1, CELF-2, Drosophila melanogaster Bruno protein and similar proteins
    cd12640
    Location:516 → 594
    RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
    TIGR01661
    Location:153 → 602
    ELAV_HUD_SF; ELAV/HuD family splicing factor
    cl17169
    Location:237 → 318
    RRM_SF; RNA recognition motif (RRM) superfamily

  11. NM_165005.3 → NP_723740.1  bruno 1, isoform D [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_723740.1

    Status: REVIEWED

    UniProtKB/TrEMBL
    G7H844, Q8SYC0
    Related
    FBpp0079929
    Conserved Domains (3) summary
    cd12640
    Location:273 → 351
    RRM3_Bruno_like; RNA recognition motif 3 in Drosophila melanogaster Bruno protein and similar proteins
    TIGR01661
    Location:2 → 359
    ELAV_HUD_SF; ELAV/HuD family splicing factor
    cl17169
    Location:1 → 75
    RRM_SF; RNA recognition motif (RRM) superfamily
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
M9PDB0 M9PDB0
O02374 O02374
O18409 O18409
Q8IP89 Q8IP89
Q8IP90 Q8IP90
Q8MSG0 Q8MSG0
Q8SYC0 Q8SYC0
Q960Z4 Q960Z4

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