Dbh dopamine beta-hydroxylase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Dbhprovided by RGD
Official Full Name: dopamine beta-hydroxylaseprovided by RGD
Primary source: RGD:2489
See related: Ensembl:ENSRNOG00000006641 AllianceGenome:RGD:2489
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: DOPBHY
Summary: Enables copper ion binding activity and dopamine beta-monooxygenase activity. Involved in several processes, including cellular biogenic amine metabolic process; cellular response to manganese ion; and cellular response to nicotine. Located in several cellular components, including axon; dendrite; and neuronal cell body. Biomarker of brain ischemia; hypertension; mental depression; and portal hypertension. Human ortholog(s) of this gene implicated in several diseases, including alcohol use disorder; attention deficit hyperactivity disorder; dopamine beta-hydroxylase deficiency; hypertension; and migraine with aura. Orthologous to human DBH (dopamine beta-hydroxylase). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward (RPKM 642.7) See more
Orthologs: human mouse all
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Genomic context

Location:: 3p12

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (30886313..30903313)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (10488260..10505245)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (5709236..5731895)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Study revealed age-related changes in tyrosine hydroxylase and DBH immunoreactivities in the carotid body glomus cells of spontaneously hypertensive (SHR/Izm) and Wistar Kyoto (WKY/Izm) rats at 4, 8, 12, and 16 weeks of age using immunohistochemistry. DBH age-dependently increased in the glomus cells of SHR/Izm in contrast to WKY/Izm.
Title: Age-related changes in immunoreactivity for dopamine β-hydroxylase in carotid body glomus cells in spontaneously hypertensive rats.
tyrosine hydroxylase and dopamine beta-hydroxylase-was evaluated in the organ of Zuckerkandl of rats in the critical period of morphogenesis
Title: Gene expression and content of enzymes of noradrenaline synthesis in the rat organ of Zuckerkandl at the critical period of morphogenesis.
genetically determined decreases in adrenal expression and activity of DBH do not represent primary determinants of increased blood pressure in the SHR model
Title: Effects of transgenic expression of dopamine beta hydroxylase (Dbh) gene on blood pressure in spontaneously hypertensive rats.
With social isolation, no changes were observed in protein levels of DBH and NET in the right hippocampus, whereas expression of this norepinephrine synthetizing enzyme and transporter were elevated in the left hippocampus.
Title: Hippocampal asymmetry in expression of catecholamine synthesizing enzyme and transporters in socially isolated rats.
Data suggest that the number of glomus cells with strong DBH immunoreactivity is increased in carotid bodies in spontaneously hypertensive rats compared with control rats; DBH is mainly observed in nerve fibers around blood vessels in control rats.
Title: Increased total volume and dopamine β-hydroxylase immunoreactivity of carotid body in spontaneously hypertensive rats.
Transcripts encoding catecholamine biosynthetic enzyme dopamine-beta-hydroxylase are unaffected by acute insulin treatment but are diminished by serial insulin dosing in hypoglycemic rats.
Title: Quantitative RT-PCR and immunoblot analyses reveal acclimated A2 noradrenergic neuron substrate fuel transporter, glucokinase, phospho-AMPK, and dopamine-β-hydroxylase responses to hypoglycemia.
Stress did not alter dopamine beta-hydroxylase expression in heart ventricles.
Title: Regulation of catecholamine-synthesising enzymes and beta-adrenoceptors gene expression in ventricles of stressed rats.
The present study demonstrates an upregulatory effect of Phox2a and Phox2b on the expression of dopamine beta-hydroxylase and norepinephrine transporter in noradrenergic neurons of rat brains
Title: Transcription factor Phox2 upregulates expression of norepinephrine transporter and dopamine β-hydroxylase in adult rat brains.
findings indicate two different mechanisms whereby DBH transcription is regulated by E(2) in the presence of ERalpha.
Title: Varied mechanisms of oestradiol-mediated regulation of dopamine β-hydroxylase transcription.
Psychosocial stress produced a significant increase of both TH mRNA and DBH mRNA levels in stellate ganglia.
Title: Psychosocial stress-related changes in gene expression of norepinephrine biosynthetic enzymes in stellate ganglia of adult rats.

Submit: New GeneRIF Correction See all GeneRIFs (17)

General gene information

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Markers

RH135117 (e-PCR)
- UniSTS:218394

Dbh (e-PCR), detects polymorphism
- UniSTS:142834

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables L-ascorbic acid binding	IEA Inferred from Electronic Annotation more info
enables copper ion binding	IBA Inferred from Biological aspect of Ancestor more info
enables copper ion binding	IEA Inferred from Electronic Annotation more info
enables copper ion binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables copper ion binding	ISO Inferred from Sequence Orthology more info
enables copper ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables dopamine beta-monooxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables dopamine beta-monooxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables dopamine beta-monooxygenase activity	IEA Inferred from Electronic Annotation more info
enables dopamine beta-monooxygenase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables dopamine beta-monooxygenase activity	ISO Inferred from Sequence Orthology more info
enables dopamine beta-monooxygenase activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within associative learning	ISO Inferred from Sequence Orthology more info
involved_in behavioral response to ethanol	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within behavioral response to ethanol	ISO Inferred from Sequence Orthology more info
involved_in blood vessel remodeling	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within blood vessel remodeling	ISO Inferred from Sequence Orthology more info
involved_in bone development	IEP Inferred from Expression Pattern more info	PubMed
involved_in catecholamine metabolic process	IC Inferred by Curator more info	PubMed
involved_in cellular response to manganese ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to nicotine	IEP Inferred from Expression Pattern more info	PubMed
involved_in dopamine catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in dopamine catabolic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within dopamine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in dopamine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in dopamine catabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in fear response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within fear response	ISO Inferred from Sequence Orthology more info
involved_in glucose homeostasis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glucose homeostasis	ISO Inferred from Sequence Orthology more info
involved_in homoiothermy	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within homoiothermy	ISO Inferred from Sequence Orthology more info
involved_in leukocyte mediated immunity	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within leukocyte mediated immunity	ISO Inferred from Sequence Orthology more info
involved_in leukocyte migration	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within leukocyte migration	ISO Inferred from Sequence Orthology more info
involved_in locomotory behavior	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within locomotory behavior	ISO Inferred from Sequence Orthology more info
involved_in locomotory behavior	ISO Inferred from Sequence Orthology more info
involved_in maternal behavior	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within maternal behavior	ISO Inferred from Sequence Orthology more info
involved_in memory	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within memory	ISO Inferred from Sequence Orthology more info
involved_in norepinephrine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in norepinephrine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in norepinephrine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within norepinephrine biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in norepinephrine biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in norepinephrine biosynthetic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in norepinephrine biosynthetic process	TAS Traceable Author Statement more info	PubMed
involved_in octopamine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in octopamine metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cold-induced thermogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cold-induced thermogenesis	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in positive regulation of vasoconstriction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within positive regulation of vasoconstriction	ISO Inferred from Sequence Orthology more info
involved_in regulation of extrinsic apoptotic signaling pathway	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of extrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in regulation of vascular associated smooth muscle cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of vascular associated smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in regulation of vascular endothelial cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of vascular endothelial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in response to amphetamine	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to amphetamine	ISO Inferred from Sequence Orthology more info
involved_in response to copper ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to estradiol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to ethanol	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to immobilization stress	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to iron ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to isolation stress	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to organic cyclic compound	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to ozone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to pain	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to pain	ISO Inferred from Sequence Orthology more info
involved_in response to peptide hormone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in social behavior	IEP Inferred from Expression Pattern more info	PubMed
involved_in vasoconstriction	IEA Inferred from Electronic Annotation more info
acts_upstream_of vasoconstriction	ISO Inferred from Sequence Orthology more info
involved_in visual learning	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within visual learning	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in apical part of cell	IDA Inferred from Direct Assay more info	PubMed
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in centriolar satellite	IEA Inferred from Electronic Annotation more info
located_in centriolar satellite	ISO Inferred from Sequence Orthology more info
located_in chromaffin granule lumen	IEA Inferred from Electronic Annotation more info
located_in chromaffin granule membrane	IEA Inferred from Electronic Annotation more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IEA Inferred from Electronic Annotation more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in extracellular space	ISS Inferred from Sequence or Structural Similarity more info
located_in intracellular membrane-bounded organelle	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in secretory granule lumen	ISO Inferred from Sequence Orthology more info
located_in secretory granule lumen	ISS Inferred from Sequence or Structural Similarity more info
is_active_in secretory granule membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in secretory granule membrane	ISO Inferred from Sequence Orthology more info
located_in secretory granule membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed
located_in transport vesicle membrane	IEA Inferred from Electronic Annotation more info
located_in varicosity	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: dopamine beta-hydroxylase

Names: Dopamine beta hydroxylase (dopamine beta-monooxygenase); dopamine beta-monooxygenase

NP_037290.3

EC 1.14.17.1

XP_063139234.1

EC 1.14.17.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_013158.3 → NP_037290.3 dopamine beta-hydroxylase precursor

Status: VALIDATED

Source sequence(s)

JAXUCZ010000003

UniProtKB/Swiss-Prot

Q05754

UniProtKB/TrEMBL

A6JTK7, G3V9S4

Related

ENSRNOP00000057915.1, ENSRNOT00000061201.4

Conserved Domains (3) summary

pfam01082
Location:217 → 340

Cu2_monooxygen; Copper type II ascorbate-dependent monooxygenase, N-terminal domain

pfam03351
Location:60 → 175

DOMON; DOMON domain

pfam03712
Location:363 → 519

Cu2_monoox_C; Copper type II ascorbate-dependent monooxygenase, C-terminal domain