Pkm pyruvate kinase M1/2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pkmprovided by RGD
Official Full Name: pyruvate kinase M1/2provided by RGD
Primary source: RGD:3337
See related: Ensembl:ENSRNOG00000011329 AllianceGenome:RGD:3337
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PK; Pk3; Pkm2; PKM12
Summary: Enables several functions, including ADP binding activity; pyruvate kinase activity; and thyroid hormone binding activity. Involved in several processes, including ATP biosynthetic process; animal organ regeneration; and pyruvate metabolic process. Part of pyruvate kinase complex. Orthologous to human PKM (pyruvate kinase M1/2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Muscle (RPKM 9449.2), Heart (RPKM 2392.1) and 6 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 8q24

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	8	NC_086026.1 (68949731..68975394)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	8	NC_051343.1 (60057629..60079600)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	8	NC_005107.4 (64480963..64502957)

Chromosome 8 - NC_086026.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nuclear translocation of metabolic enzyme PKM2 participates in high glucose-promoted HCC metastasis by strengthening immunosuppressive environment.
Title: Nuclear translocation of metabolic enzyme PKM2 participates in high glucose-promoted HCC metastasis by strengthening immunosuppressive environment.
Silencing PKM2 Attenuates Brain Injury Induced by Status Epilepticus by Inhibiting the AKT/mTOR Pathway and the NLRP3 Inflammasome.
Title: Silencing PKM2 Attenuates Brain Injury Induced by Status Epilepticus by Inhibiting the AKT/mTOR Pathway and the NLRP3 Inflammasome.
Inhibition of Pyruvate kinase M2 (PKM2) by shikonin attenuates isoproterenol-induced acute myocardial infarction via reduction in inflammation, hypoxia, apoptosis, and fibrosis.
Title: Inhibition of Pyruvate kinase M2 (PKM2) by shikonin attenuates isoproterenol-induced acute myocardial infarction via reduction in inflammation, hypoxia, apoptosis, and fibrosis.
M-type pyruvate kinase 2 (PKM2) tetramerization alleviates the progression of right ventricle failure by regulating oxidative stress and mitochondrial dynamics.
Title: M-type pyruvate kinase 2 (PKM2) tetramerization alleviates the progression of right ventricle failure by regulating oxidative stress and mitochondrial dynamics.
Pyruvate Kinase M2: A New Biomarker for the Early Detection of Diabetes-Induced Nephropathy.
Title: Pyruvate Kinase M2: A New Biomarker for the Early Detection of Diabetes-Induced Nephropathy.
Shikonin improves pulmonary vascular remodeling in monocrotalineinduced pulmonary arterial hypertension via regulation of PKM2.
Title: Shikonin improves pulmonary vascular remodeling in monocrotaline‑induced pulmonary arterial hypertension via regulation of PKM2.
Downregulation of miR-122-5p Activates Glycolysis via PKM2 in Kupffer Cells of Rat and Mouse Models of Non-Alcoholic Steatohepatitis.
Title: Downregulation of miR-122-5p Activates Glycolysis via PKM2 in Kupffer Cells of Rat and Mouse Models of Non-Alcoholic Steatohepatitis.
Platelet-Derived TGF (Transforming Growth Factor)-beta1 Enhances the Aerobic Glycolysis of Pulmonary Arterial Smooth Muscle Cells by PKM2 (Pyruvate Kinase Muscle Isoform 2) Upregulation.
Title: Platelet-Derived TGF (Transforming Growth Factor)-β1 Enhances the Aerobic Glycolysis of Pulmonary Arterial Smooth Muscle Cells by PKM2 (Pyruvate Kinase Muscle Isoform 2) Upregulation.
Bile acids attenuate PKM2 pathway activation in proinflammatory microglia.
Title: Bile acids attenuate PKM2 pathway activation in proinflammatory microglia.
Hyperpolarized pyruvate to measure the influence of PKM2 activation on glucose metabolism in the healthy kidney.
Title: Hyperpolarized pyruvate to measure the influence of PKM2 activation on glucose metabolism in the healthy kidney.

Submit: New GeneRIF Correction See all GeneRIFs (30)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH94821 (e-PCR)
- UniSTS:120067

Pkm2 (e-PCR), detects polymorphism
- UniSTS:507144

Pkm2 (e-PCR), detects polymorphism
- UniSTS:516648

Pkm2 (e-PCR), detects polymorphism
- UniSTS:516649

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ADP binding	IDA Inferred from Direct Assay more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
NOT enables histone H3T11 kinase activity	ISO Inferred from Sequence Orthology more info
enables histone H3T11 kinase activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	IDA Inferred from Direct Assay more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables mRNA binding	ISO Inferred from Sequence Orthology more info
enables mRNA binding	ISS Inferred from Sequence or Structural Similarity more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables potassium ion binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein dimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables protein tyrosine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein tyrosine kinase activity	ISO Inferred from Sequence Orthology more info
enables pyruvate kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables pyruvate kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables pyruvate kinase activity	IEA Inferred from Electronic Annotation more info
enables pyruvate kinase activity	ISO Inferred from Sequence Orthology more info
enables thyroid hormone binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription coactivator activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in animal organ regeneration	IDA Inferred from Direct Assay more info	PubMed
involved_in canonical glycolysis	ISO Inferred from Sequence Orthology more info
involved_in cellular response to insulin stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in glucose metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycolytic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glycolytic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glycolytic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within glycolytic process	ISO Inferred from Sequence Orthology more info
involved_in glycolytic process	ISO Inferred from Sequence Orthology more info
involved_in liver development	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cytoplasmic translation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cytoplasmic translation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of sprouting angiogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in programmed cell death	ISO Inferred from Sequence Orthology more info
involved_in protein homotetramerization	IDA Inferred from Direct Assay more info	PubMed
involved_in protein tetramerization	IDA Inferred from Direct Assay more info	PubMed
involved_in pyruvate biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in pyruvate metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in response to gravity	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to hypoxia	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to insulin	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to muscle inactivity	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to nutrient	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to organic substance	IEP Inferred from Expression Pattern more info	PubMed
involved_in skeletal muscle tissue regeneration	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cilium	ISO Inferred from Sequence Orthology more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in photoreceptor inner segment	ISO Inferred from Sequence Orthology more info
part_of pyruvate kinase complex	IDA Inferred from Direct Assay more info	PubMed
located_in rough endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in rough endoplasmic reticulum	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: pyruvate kinase PKM

Names: M2 pyruvate kinase; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; pyruvate kinase, muscle; threonine-protein kinase PKM2; tyrosine-protein kinase PKM2

NP_445749.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_006243250.3

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_006243252.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_063121023.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_063121024.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_063121025.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_063121026.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_063121027.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_063121028.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_063121030.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_063121031.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_063121032.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_063121033.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

XP_063121034.1

EC 2.7.1.40

EC 2.7.10.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_053297.2 → NP_445749.1 pyruvate kinase PKM

See identical proteins and their annotated locations for NP_445749.1

Status: PROVISIONAL

Source sequence(s)

X15800

UniProtKB/Swiss-Prot

P11980, P11981

UniProtKB/TrEMBL

A0A8L2Q7B9, A6J531

Conserved Domains (1) summary

cd00288
Location:42 → 530

Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086026.1 Reference GRCr8

Range

68949731..68975394

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006243188.4 → XP_006243250.3 pyruvate kinase PKM isoform X7
XM_063264954.1 → XP_063121024.1 pyruvate kinase PKM isoform X2
XM_063264958.1 → XP_063121028.1 pyruvate kinase PKM isoform X6
XM_063264953.1 → XP_063121023.1 pyruvate kinase PKM isoform X1
XM_063264957.1 → XP_063121027.1 pyruvate kinase PKM isoform X5
XM_063264961.1 → XP_063121031.1 pyruvate kinase PKM isoform X10

UniProtKB/Swiss-Prot

P11980, P11981

UniProtKB/TrEMBL

A6J531
XM_006243190.4 → XP_006243252.1 pyruvate kinase PKM isoform X8

UniProtKB/TrEMBL

A0A8I6ABE3, A0A8L2Q7B9

Related

ENSRNOP00000089941.1

Conserved Domains (2) summary

cd00288
Location:102 → 590

Pyruvate_Kinase; Pyruvate kinase (PK): Large allosteric enzyme that regulates glycolysis through binding of the substrate, phosphoenolpyruvate, and one or more allosteric effectors. Like other allosteric enzymes, PK has a high substrate affinity R state and a low ...

TIGR01064
Location:103 → 587

pyruv_kin; pyruvate kinase
XM_063264960.1 → XP_063121030.1 pyruvate kinase PKM isoform X9

UniProtKB/TrEMBL

A0A8I6G5T6
XM_063264962.1 → XP_063121032.1 pyruvate kinase PKM isoform X10

UniProtKB/Swiss-Prot

P11980, P11981

UniProtKB/TrEMBL

A6J531
XM_063264963.1 → XP_063121033.1 pyruvate kinase PKM isoform X11

UniProtKB/TrEMBL

A6J532
XM_063264956.1 → XP_063121026.1 pyruvate kinase PKM isoform X4
XM_063264955.1 → XP_063121025.1 pyruvate kinase PKM isoform X3
XM_063264964.1 → XP_063121034.1 pyruvate kinase PKM isoform X12