Cyp24a1 cytochrome P450, family 24, subfamily a, polypeptide 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cyp24a1provided by RGD
Official Full Name: cytochrome P450, family 24, subfamily a, polypeptide 1provided by RGD
Primary source: RGD:2462
See related: Ensembl:ENSRNOG00000013062 AllianceGenome:RGD:2462
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Cyp24; VITD12; 24-OHase; 24R-Ohase; P450-CC24
Summary: Enables 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity; 25-hydroxycholecalciferol-24-hydroxylase activity; and vitamin D 25-hydroxylase activity. Involved in vitamin D catabolic process. Predicted to be located in mitochondrion and nucleoplasm. Human ortholog(s) of this gene implicated in hypercalcemia. Orthologous to human CYP24A1 (cytochrome P450 family 24 subfamily A member 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward (RPKM 83.5) See more
Orthologs: human mouse all
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Genomic context

Location:: 3q42

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (179694647..179709083, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (159275947..159290383, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (168097484..168111920, complement)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Altered expression of the vitamin D metabolizing enzymes CYP27B1 and CYP24A1 under the context of prostate aging and pathologies.
Title: Altered expression of the vitamin D metabolizing enzymes CYP27B1 and CYP24A1 under the context of prostate aging and pathologies.
Ezh2-dependent H3K27me3 modification dynamically regulates vitamin D3-dependent epigenetic control of CYP24A1 gene expression in osteoblastic cells.
Title: Ezh2-dependent H3K27me3 modification dynamically regulates vitamin D3-dependent epigenetic control of CYP24A1 gene expression in osteoblastic cells.
Uraemic rats exhibit significant upregulation in basal CYP24 mRNA and protein expression, coupled with decreased miR-125b expression, which may have an impact on vitamin D status.
Title: Loss of miR-125b contributes to upregulation of CYP24 in uraemic rats.
liver has the capacity for an active vitamin D catabolism in different populations of liver cells, especially in sinusoidal endothelial cells, through an expression of CYP24.
Title: Expression pattern of CYP24 in liver during ageing in long-term diabetes.
Findings suggest that CYP24A1 protein plays an important role in the relationship between diabetic peripheral neuropathy and calcitriol.
Title: High glucose upregulates CYP24A1 expression which attenuates the ability of 1,25(OH)2D3 to increase NGF secretion in a rat Schwann cell line RSC96.
Human and rat CYP24A1 metabolize 20-hydroxyvitamin D3 to the same dihydroxyvitamin D3 products.
Title: Metabolism of 20-hydroxyvitamin D3 and 20,23-dihydroxyvitamin D3 by rat and human CYP24A1.
Magnesium deficiency induces downregulation of 1alpha(OH)ase and type IIa and IIc Na/Pi cotransporters, and upregulation of 24(OH)ase in the kidney.
Title: Magnesium deficiency regulates vitamin D metabolizing enzymes and type II sodium-phosphate cotransporter mRNA expression in rats.
The CYP24A1 structure provides the first template for understanding membrane insertion, substrate binding, and redox partner interaction in mitochondrial P450s.
Title: Crystal structure of CYP24A1, a mitochondrial cytochrome P450 involved in vitamin D metabolism.
When compared to benign granulose GLZ cells, the malignant HGL5 cells showed a significantly higher 24-OHase expression at the protein level
Title: Expression of 25-hydroxyvitamin D3-24-hydroxylase in benign and malignant ovarian cell lines and tissue.
The identification of a site in the CYP24 proximal promoter that is critical for 1,25-D induction of CYP24.
Title: Molecular action of 1,25-dihydroxyvitamin D3 and phorbol ester on the activation of the rat cytochrome P450C24 (CYP24) promoter: role of MAP kinase activities and identification of an important transcription factor binding site.

Submit: New GeneRIF Correction See all GeneRIFs (19)

General gene information

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Markers

PMC61082P1 (e-PCR)
- UniSTS:273409

Cyp24a1 (e-PCR), detects polymorphism
- UniSTS:516654

Cyp24 (e-PCR), detects polymorphism
- UniSTS:141449

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables 1-alpha,25-dihydroxyvitamin D3 23-hydroxylase activity	ISO Inferred from Sequence Orthology more info
enables 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity	IDA Inferred from Direct Assay more info	PubMed
enables 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity	ISO Inferred from Sequence Orthology more info
enables 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity	TAS Traceable Author Statement more info	PubMed
enables 25-hydroxycholecalciferol-23-hydroxylase activity	ISO Inferred from Sequence Orthology more info
enables 25-hydroxycholecalciferol-24-hydroxylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 25-hydroxycholecalciferol-24-hydroxylase activity	IDA Inferred from Direct Assay more info	PubMed
enables 25-hydroxycholecalciferol-24-hydroxylase activity	ISO Inferred from Sequence Orthology more info
enables 25-hydroxycholecalciferol-24-hydroxylase activity	TAS Traceable Author Statement more info	PubMed
enables heme binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables monooxygenase activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen	IEA Inferred from Electronic Annotation more info
enables vitamin D 24-hydroxylase activity	IDA Inferred from Direct Assay more info	PubMed
enables vitamin D 25-hydroxylase activity	IBA Inferred from Biological aspect of Ancestor more info
enables vitamin D 25-hydroxylase activity	IDA Inferred from Direct Assay more info	PubMed
enables vitamin D 25-hydroxylase activity	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in cellular response to vitamin D	IEP Inferred from Expression Pattern more info	PubMed
involved_in osteoblast differentiation	ISO Inferred from Sequence Orthology more info
involved_in response to vitamin D	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to vitamin D	ISO Inferred from Sequence Orthology more info
involved_in steroid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in vitamin D catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in vitamin D catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in vitamin D catabolic process	ISO Inferred from Sequence Orthology more info
involved_in vitamin D catabolic process	TAS Traceable Author Statement more info	PubMed
involved_in vitamin D metabolic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within vitamin D metabolic process	ISO Inferred from Sequence Orthology more info
involved_in vitamin D metabolic process	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial

Names: 25-hydroxyvitamin D3 24-hydroxylase; 25-hydroxyvitaminD324-hydroxylase; Cytochrom P450 (25-hydroxyvitamin D24-hydroxylase); cytochrome P450 24A1; cytochrome P450, subfamily 24; cytochrome P450-CC24; vitamin D(3) 24-hydroxylase

NP_963966.1

EC 1.14.15.16

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_201635.3 → NP_963966.1 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial precursor

See identical proteins and their annotated locations for NP_963966.1

Status: VALIDATED

Source sequence(s)

BC100059

UniProtKB/Swiss-Prot

Q09128, Q498V4, Q63685

UniProtKB/TrEMBL

A0A8I6A8M6, A6JXS6

Related

ENSRNOP00000043298.2, ENSRNOT00000046011.3

Conserved Domains (1) summary

cd20645
Location:90 → 508

CYP24A1; cytochrome P450 family 24, subfamily A, polypeptide 1, also called vitamin D(3) 24-hydroxylase