Prkca protein kinase C, alpha [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Prkcaprovided by RGD
Official Full Name: protein kinase C, alphaprovided by RGD
Primary source: RGD:3395
See related: Ensembl:ENSRNOG00000003491 AllianceGenome:RGD:3395
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Pkca
Summary: Enables calcium-dependent protein kinase C activity. Involved in several processes, including positive regulation of ERK1 and ERK2 cascade; protein phosphorylation; and regulation of vesicle-mediated transport. Located in several cellular components, including membrane raft; mitochondrion; and perinuclear region of cytoplasm. Part of protein-containing complex. Used to study hypertension. Biomarker of brain ischemia; congestive heart failure; and ischemia. Human ortholog(s) of this gene implicated in dilated cardiomyopathy; high grade glioma (multiple); large cell carcinoma; and reproductive organ cancer (multiple). Orthologous to human PRKCA (protein kinase C alpha). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Muscle (RPKM 146.0), Brain (RPKM 140.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 10q32.1

Exon count:: 18

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	10	NC_086028.1 (93388991..93787617, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	10	NC_051345.1 (92889390..93288013, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	10	NC_005109.4 (96186509..96585168, complement)

Chromosome 10 - NC_086028.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Distinct protein kinase C isoforms drive the cell cycle re-entry of two separate populations of neonatal rat ventricular cardiomyocytes.
Title: Distinct protein kinase C isoforms drive the cell cycle re-entry of two separate populations of neonatal rat ventricular cardiomyocytes.
Spatiotemporal distribution of PKCalpha, Cdc42, and Rac1 before directed cell migration.
Title: Spatiotemporal distribution of PKCα, Cdc42, and Rac1 before directed cell migration.
miR-709 inhibits GHRP6 induced GH synthesis by targeting PRKCA in pituitary.
Title: miR-709 inhibits GHRP6 induced GH synthesis by targeting PRKCA in pituitary.
Cross-Talk of Toll-Like Receptor 5 and Mu-Opioid Receptor Attenuates Chronic Constriction Injury-Induced Mechanical Hyperalgesia through a Protein Kinase C Alpha-Dependent Signaling.
Title: Cross-Talk of Toll-Like Receptor 5 and Mu-Opioid Receptor Attenuates Chronic Constriction Injury-Induced Mechanical Hyperalgesia through a Protein Kinase C Alpha-Dependent Signaling.
Upregulation of miR-92a-2-5p potentially contribute to anorectal malformations by inhibiting proliferation and enhancing apoptosis via PRKCA/beta-catenin.
Title: Upregulation of miR-92a-2-5p potentially contribute to anorectal malformations by inhibiting proliferation and enhancing apoptosis via PRKCA/β-catenin.
PKCalpha mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism.
Title: PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism.
MicroRNA-219a-5p-mediated inhibition of CaMKIIgamma facilitates vestibular compensation in acute vertigo by promoting protein kinase C expression.
Title: MicroRNA-219a-5p-mediated inhibition of CaMKIIγ facilitates vestibular compensation in acute vertigo by promoting protein kinase C expression.
Cardiac contractile dysfunction and protein kinase C-mediated myofilament phosphorylation in disease and aging.
Title: Cardiac contractile dysfunction and protein kinase C-mediated myofilament phosphorylation in disease and aging.
we demonstrate that ULK1 is phosphorylated at Ser317/555/777 and Raptor is also phosphorylated by phospho-PKCalpha. Phospho-ULK1 (p-ULK1) at these sites are all essential for PEDF-induced mitophagy and reduce the release of mitochondrial ROS and DNA.
Title: PKCα replaces AMPK to regulate mitophagy: Another PEDF role on ischaemic cardioprotection.
Structural Basis of Protein Kinase Calpha Regulation by the C-Terminal Tail
Title: Structural Basis of Protein Kinase Cα Regulation by the C-Terminal Tail.

Submit: New GeneRIF Correction See all GeneRIFs (183)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH94850 (e-PCR)
- UniSTS:120096

RH94851 (e-PCR)
- UniSTS:120097

Prkca (e-PCR), detects polymorphism
- UniSTS:144191

Prkca (e-PCR), detects polymorphism
- UniSTS:144193

d10rat139 (e-PCR)
- UniSTS:120655

Prkca (e-PCR), detects polymorphism
- UniSTS:239206

d10rat189 (e-PCR)
- UniSTS:120689

fk85g05.x1 (e-PCR)
- UniSTS:201633

D10Rat53 (e-PCR)
- UniSTS:118194

D10Chm19 (e-PCR)
- UniSTS:518110

D10Chm12 (e-PCR)
- UniSTS:518112

RH137792 (e-PCR)
- UniSTS:219879

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables diacylglycerol-dependent serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables diacylglycerol-dependent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables enzyme binding	IEA Inferred from Electronic Annotation more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables histone H3T6 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T6 kinase activity	ISO Inferred from Sequence Orthology more info
enables integrin binding	ISO Inferred from Sequence Orthology more info
enables lipid binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables zinc ion binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
acts_upstream_of cell population proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to carbohydrate stimulus	ISO Inferred from Sequence Orthology more info
involved_in central nervous system neuron axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chondrocyte differentiation	ISO Inferred from Sequence Orthology more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in desmosome assembly	IEA Inferred from Electronic Annotation more info
involved_in desmosome assembly	ISO Inferred from Sequence Orthology more info
involved_in establishment of protein localization	TAS Traceable Author Statement more info	PubMed
acts_upstream_of_or_within induction of positive chemotaxis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within intracellular calcium ion homeostasis	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within intrinsic apoptotic signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in learning or memory	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within muscle cell cellular homeostasis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of MAPK cascade	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glial cell apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of glial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glial cell apoptotic process	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within negative regulation of glucose import	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of insulin receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of translation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neutrophil chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-serine phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of ERK1 and ERK2 cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of angiogenesis	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of angiogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of angiotensin-activated signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of angiotensin-activated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of blood vessel endothelial cell migration	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of blood vessel endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of bone resorption	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cardiac muscle hypertrophy	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell adhesion	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell adhesion	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of dense core granule biogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of dense core granule biogenesis	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endothelial cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of endothelial cell proliferation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of endothelial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endothelial cell proliferation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of inflammatory response	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of lipopolysaccharide-mediated signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of lipopolysaccharide-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of lipopolysaccharide-mediated signaling pathway	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of macrophage differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of macrophage differentiation	ISS Inferred from Sequence or Structural Similarity more info
involved_in positive regulation of mitotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of mitotic cell cycle	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mitotic cell cycle	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within positive regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of smooth muscle cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of synapse assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in post-translational protein modification	IEA Inferred from Electronic Annotation more info
involved_in post-translational protein modification	ISO Inferred from Sequence Orthology more info
involved_in presynaptic modulation of chemical synaptic transmission	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of muscle contraction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of peptidyl-tyrosine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of platelet aggregation	IEA Inferred from Electronic Annotation more info
involved_in regulation of platelet aggregation	ISO Inferred from Sequence Orthology more info
involved_in regulation of platelet aggregation	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of receptor-mediated endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of response to osmotic stress	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic vesicle exocytosis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of the force of heart contraction	ISO Inferred from Sequence Orthology more info
involved_in response to corticosterone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to estradiol	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to ethanol	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to interleukin-1	IEA Inferred from Electronic Annotation more info
involved_in response to interleukin-1	ISO Inferred from Sequence Orthology more info
involved_in response to mechanical stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to organic cyclic compound	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to peptide hormone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to reactive oxygen species	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to toxic substance	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within stem cell differentiation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of alphav-beta3 integrin-PKCalpha complex	ISO Inferred from Sequence Orthology more info
located_in apical part of cell	ISO Inferred from Sequence Orthology more info
located_in axon	ISO Inferred from Sequence Orthology more info
is_active_in calyx of Held	ISO Inferred from Sequence Orthology more info
located_in ciliary basal body	IEA Inferred from Electronic Annotation more info
is_active_in ciliary basal body	ISO Inferred from Sequence Orthology more info
located_in cone photoreceptor outer segment	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in intercalated disc	ISO Inferred from Sequence Orthology more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in mitochondrial membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in ooplasm	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in presynapse	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic cytosol	ISO Inferred from Sequence Orthology more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: protein kinase C alpha type

Names: PKC-A; PKC-alpha

NP_001386228.1

EC 2.7.11.13

XP_017452495.2

EC 2.7.11.13

XP_038941139.1

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001399299.2 → NP_001386228.1 protein kinase C alpha type

Status: VALIDATED

Source sequence(s)

JAXUCZ010000010

UniProtKB/Swiss-Prot

P05696

UniProtKB/TrEMBL

B5DFC4

Related

ENSRNOP00000004699.8, ENSRNOT00000004699.9

Conserved Domains (4) summary

cd04026
Location:159 → 289

C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and gamma

cd05615
Location:328 → 668

STKc_cPKC_alpha; Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha

cd20833
Location:35 → 92

C1_cPKC_rpt1; first protein kinase C conserved region 1 (C1 domain) found in the classical (or conventional) protein kinase C (cPKC) family

cd20836
Location:102 → 155

C1_cPKC_rpt2; second protein kinase C conserved region 1 (C1 domain) found in the classical (or conventional) protein kinase C (cPKC) family

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086028.1 Reference GRCr8

Range

93388991..93787617 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_017597006.3 → XP_017452495.2 protein kinase C alpha type isoform X2

UniProtKB/TrEMBL

A0A8I5ZMR6, F1LS98

Related

ENSRNOP00000079110.1

Conserved Domains (4) summary

cd04026
Location:159 → 289

C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and gamma

cd05615
Location:328 → 684

STKc_cPKC_alpha; Catalytic domain of the Serine/Threonine Kinase, Classical Protein Kinase C alpha

cd20833
Location:35 → 92

C1_cPKC_rpt1; first protein kinase C conserved region 1 (C1 domain) found in the classical (or conventional) protein kinase C (cPKC) family

cd20836
Location:102 → 155

C1_cPKC_rpt2; second protein kinase C conserved region 1 (C1 domain) found in the classical (or conventional) protein kinase C (cPKC) family
XM_039085211.2 → XP_038941139.1 protein kinase C alpha type isoform X1

UniProtKB/TrEMBL

F1LS98

Conserved Domains (4) summary

cd04026
Location:159 → 289

C2_PKC_alpha_gamma; C2 domain in Protein Kinase C (PKC) alpha and gamma

cd20833
Location:35 → 92

C1_cPKC_rpt1; first protein kinase C conserved region 1 (C1 domain) found in the classical (or conventional) protein kinase C (cPKC) family

cd20836
Location:102 → 155

C1_cPKC_rpt2; second protein kinase C conserved region 1 (C1 domain) found in the classical (or conventional) protein kinase C (cPKC) family

cl21453
Location:328 → 718

PKc_like; Protein Kinases, catalytic domain

Suppressed Reference Sequence(s)

The following Reference Sequences have been suppressed. Explain

NM_001105713.1: Suppressed sequence

Description

NM_001105713.1: This RefSeq was removed because currently there is insufficient support for the transcript and the protein.