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Ldhb lactate dehydrogenase B [ Rattus norvegicus (Norway rat) ]

Gene ID: 24534, updated on 5-Mar-2024

Summary

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Official Symbol
Ldhbprovided by RGD
Official Full Name
lactate dehydrogenase Bprovided by RGD
Primary source
RGD:2997
See related
Ensembl:ENSRNOG00000013000 AllianceGenome:RGD:2997
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
Ldh2
Summary
This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 9. [provided by RefSeq, Feb 2016]
Expression
Biased expression in Heart (RPKM 12336.6), Kidney (RPKM 2578.0) and 3 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Ldhb in Genome Data Viewer
Location:
4q44
Exon count:
9
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 4 NC_086022.1 (177159389..177177408, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 4 NC_051339.1 (175428382..175446403, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 4 NC_005103.4 (176701980..176719999, complement)

Chromosome 4 - NC_086022.1Genomic Context describing neighboring genes Neighboring gene spexin hormone Neighboring gene glycogen synthase 2 Neighboring gene potassium inwardly-rectifying channel, subfamily J, member 8 Neighboring gene ATP binding cassette subfamily C member 9

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. MicroRNA-375-3p inhibitor suppresses angiotensin II-induced cardiomyocyte hypertrophy by promoting lactate dehydrogenase B expression. Feng H, et al. J Cell Physiol, 2019 Aug. PMID 30618075
  2. Lactate dehydrogenase regulation in aged skeletal muscle: Regulation by anabolic steroids and functional overload. Washington TA, et al. Exp Gerontol, 2014 Sep. PMID 24835193
  3. Circadian rhythm of metabolic oscillation in suprachiasmatic nucleus depends on the mitochondrial oxidation state, reflected by cytochrome C oxidase and lactate dehydrogenase. Isobe Y, et al. J Neurosci Res, 2011 Jun. PMID 21416482
  4. Purification and radioimmunoassay of rat lactate dehydrogenase A and B subunits. Beebee TJ, et al. Biochem J, 1982 Aug 1. PMID 7138505, Free PMC Article
  5. Mammalian oocytes: X chromosome activity. Epstein CJ. Science, 1969 Mar 7. PMID 5764873

See all (36) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. miR-375-3p inhibitor suppresses Ang II-induced cardiomyocyte hypertrophy by promoting LDHB expression.
    Title: MicroRNA-375-3p inhibitor suppresses angiotensin II-induced cardiomyocyte hypertrophy by promoting lactate dehydrogenase B expression.
  2. The readthrough-extended lactate dehydrogenase subunit LDHBx can also co-import LDHA, the other LDH subunit, into peroxisomes.
    Title: Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.
  3. aging alters aspects of skeletal muscle isoform shift induced by overload and anabolic steroid administration
    Title: Lactate dehydrogenase regulation in aged skeletal muscle: Regulation by anabolic steroids and functional overload.
  4. LDH and its coding mRNA were higher during the dark period.
    Title: Circadian rhythm of metabolic oscillation in suprachiasmatic nucleus depends on the mitochondrial oxidation state, reflected by cytochrome C oxidase and lactate dehydrogenase.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables L-lactate dehydrogenase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables L-lactate dehydrogenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables L-lactate dehydrogenase activity ISO
Inferred from Sequence Orthology
more info
  
enables NAD binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables kinase binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables lactate dehydrogenase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in NAD metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in lactate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in lactate metabolic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in lactate metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in pyruvate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in pyruvate metabolic process ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
is_active_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in membrane raft ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial inner membrane ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrial inner membrane ISS
Inferred from Sequence or Structural Similarity
more info
  
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of oxidoreductase complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
L-lactate dehydrogenase B chain
Names
LDH heart subunit
LDH-B
LDH-H
Lactate dehydrogenease B
NP_001303262.1
NP_001303263.1
NP_036727.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001316333.1 → NP_001303262.1  L-lactate dehydrogenase B chain isoform Ldhbx

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (Ldhbx), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'.
    Source sequence(s)
    BU759234, FM054345, U07181
    UniProtKB/Swiss-Prot
    P42123
    Conserved Domains (2) summary
    cd05293
    Location:20 → 330
    LDH_1; A subgroup of L-lactate dehydrogenases
    PLN02602
    Location:4 → 332
    PLN02602; lactate dehydrogenase

  2. NM_001316334.1 → NP_001303263.1  L-lactate dehydrogenase B chain isoform Ldhb

    Status: REVIEWED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (Ldhb).
    Source sequence(s)
    BU759234, CK355620, FM054345, U07181
    UniProtKB/Swiss-Prot
    P42123
    UniProtKB/TrEMBL
    A6IMU4
    Related
    ENSRNOP00000017965.4, ENSRNOT00000017965.8
    Conserved Domains (1) summary
    cd05293
    Location:20 → 330
    LDH_1; A subgroup of L-lactate dehydrogenases

  3. NM_012595.2 → NP_036727.1  L-lactate dehydrogenase B chain isoform Ldhb

    See identical proteins and their annotated locations for NP_036727.1

    Status: REVIEWED

    Description
    Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (Ldhb), which is localized in the cytosol. Variants 1 and 2 encode the same isoform.
    Source sequence(s)
    BU759234, FM054345, U07181
    UniProtKB/Swiss-Prot
    P42123
    UniProtKB/TrEMBL
    A6IMU4
    Conserved Domains (1) summary
    cd05293
    Location:20 → 330
    LDH_1; A subgroup of L-lactate dehydrogenases

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086022.1 Reference GRCr8

    Range
    177159389..177177408 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P42123.2 GenPept UniProtKB/Swiss-Prot:P42123

Gene LinkOut

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