Sh2b2 SH2B adaptor protein 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Sh2b2provided by MGI
Official Full Name: SH2B adaptor protein 2provided by MGI
Primary source: MGI:MGI:1345171
See related: Ensembl:ENSMUSG00000005057 AllianceGenome:MGI:1345171
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Aps
Summary: Enables transmembrane receptor protein tyrosine kinase adaptor activity. Involved in intracellular signal transduction. Acts upstream of or within several processes, including B-1 B cell homeostasis; brown fat cell differentiation; and cell surface receptor signaling pathway. Located in actin filament; ruffle; and stress fiber. Is expressed in dorsal aorta. Orthologous to human SH2B2 (SH2B adaptor protein 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in mammary gland adult (RPKM 32.6), adrenal adult (RPKM 26.0) and 14 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 G2; 5 75.93 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (136247003..136275446, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (136218147..136246595, complement)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Deletion of liver SH2B1 in SH2B2 null mice attenuated very low-density lipoprotein (VLDL) secretion.
Title: Hepatic SH2B1 and SH2B2 regulate liver lipid metabolism and VLDL secretion in mice.
SH2B2beta is an endogenous inhibitor of SH2B1 and/or SH2B2alpha, negatively regulating insulin signaling and/or JAK2-mediated cellular responses.
Title: Identification of SH2B2beta as an inhibitor for SH2B1- and SH2B2alpha-promoted Janus kinase-2 activation and insulin signaling.
The association between SHIP2 and the adaptor protein Aps has an impact on biochemical properties of both partners.
Title: The association between the SH2-containing inositol polyphosphate 5-Phosphatase 2 (SHIP2) and the adaptor protein APS has an impact on biochemical properties of both partners.
APS facilitates c-Cbl tyrosine phosphorylation and GLUT4 translocation in response to insulin in 3T3-L1 adipocytes
Title: APS facilitates c-Cbl tyrosine phosphorylation and GLUT4 translocation in response to insulin in 3T3-L1 adipocytes.
These results identify Enigma as a novel APS-binding protein and suggest that the APS/Enigma complex plays a critical role in actin cytoskeleton organisation.
Title: The interaction between the adaptor protein APS and Enigma is involved in actin organisation.
APS might have a novel regulatory role in actin reorganization and control of B-1 cell compartment size.
Title: Increased numbers of B-1 cells and enhanced responses against TI-2 antigen in mice lacking APS, an adaptor molecule containing PH and SH2 domains.
Lnk, SH2-B, and APS have roles in mast cell development, growth, and functions
Title: Roles of a conserved family of adaptor proteins, Lnk, SH2-B, and APS, for mast cell development, growth, and functions: APS-deficiency causes augmented degranulation and reduced actin assembly.
Data show that the adapter protein APS preferentially associates with phosphorylated Tyr-568 and Tyr-936 of the receptor for stem-cell factor (c-Kit).
Title: The adapter protein APS associates with the multifunctional docking sites Tyr-568 and Tyr-936 in c-Kit.
APS enhanced ligand-dependent multi-ubiquitination of the insulin receptor (IR) in CHO cells.
Title: APS-mediated ubiquitination of the insulin receptor enhances its internalization, but does not induce its degradation.
Knockdown of the mouse APS gene abolished the insulin-stimulated phosphorylation of c-Cbl.
Title: Primary and essential role of the adaptor protein APS for recruitment of both c-Cbl and its associated protein CAP in insulin signaling.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

fb76e04.y1 (e-PCR)
- UniSTS:191301

Aps (e-PCR), detects polymorphism
- UniSTS:279420

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables SH2 domain binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables signaling adaptor activity	ISO Inferred from Sequence Orthology more info
enables transmembrane receptor protein tyrosine kinase adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables transmembrane receptor protein tyrosine kinase adaptor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within B cell receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in B cell receptor signaling pathway	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within B-1 B cell homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within actin cytoskeleton organization	IPI Inferred from Physical Interaction more info	PubMed
involved_in antigen receptor-mediated signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within brown fat cell differentiation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cytokine-mediated signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of establishment of localization in cell	ISA Inferred from Sequence Alignment more info	PubMed
acts_upstream_of_or_within glucose homeostasis	ISA Inferred from Sequence Alignment more info	PubMed
acts_upstream_of glucose import	ISA Inferred from Sequence Alignment more info	PubMed
acts_upstream_of_or_within insulin receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular signal transduction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of glucose import	ISA Inferred from Sequence Alignment more info	PubMed
involved_in nervous system development	ISO Inferred from Sequence Orthology more info
involved_in regulation of Ras protein signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of immune response	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in actin filament	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISS Inferred from Sequence or Structural Similarity more info	PubMed
located_in ruffle	IDA Inferred from Direct Assay more info	PubMed
located_in stress fiber	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: SH2B adapter protein 2

Names: SH2 and PH domain-containing adapter protein APS; adapter protein with pleckstrin homology and Src homology 2 domains; adaptor protein with pleckstrin homology and src homology 2 domains; insulin receptor substrate 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001302938.2 → NP_001289867.1 SH2B adapter protein 2 isoform 1

See identical proteins and their annotated locations for NP_001289867.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) uses an alternate splice site in the 5' UTR compared to variant 1. Variants 1, 2, 7 and 8 encode the same isoform (1).

Source sequence(s)

AC083890

Consensus CDS

CCDS39327.1

UniProtKB/Swiss-Prot

O88936, Q6PG00, Q9JID9

Related

ENSMUSP00000142398.2, ENSMUST00000196397.5

Conserved Domains (3) summary

cd10411
Location:401 → 496

SH2_SH2B2; Src homology 2 (SH2) domain found in SH2B adapter proteins (SH2B1, SH2B2, SH2B3)

cd01231
Location:186 → 301

PH_SH2B_family; SH2B adapter protein 1, 2, and 3 Pleckstrin homology (PH) domain

pfam08916
Location:17 → 73

Phe_ZIP; Phenylalanine zipper
NM_001302939.2 → NP_001289868.1 SH2B adapter protein 2 isoform 2

See identical proteins and their annotated locations for NP_001289868.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1.

Source sequence(s)

AC083890

Consensus CDS

CCDS80431.1

UniProtKB/TrEMBL

A0A0G2JED4

Related

ENSMUSP00000142728.2, ENSMUST00000196447.2

Conserved Domains (3) summary

cd01231
Location:186 → 301

PH_SH2B_family; SH2B adapter protein 1, 2, and 3 Pleckstrin homology (PH) domain

pfam00169
Location:204 → 295

PH; PH domain

pfam08916
Location:17 → 73

Phe_ZIP; Phenylalanine zipper
NM_001425467.1 → NP_001412396.1 SH2B adapter protein 2 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (4) uses an alternate splice site in the coding region, compared to variant 1. It encodes isoform 3 which is one amino acid shorter, compared to isoform 1.

Source sequence(s)

AC083890
NM_001425468.1 → NP_001412397.1 SH2B adapter protein 2 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (5) uses an alternate splice site in the 5' UTR and an alternate splice site in the coding region, compared to variant 1. It encodes isoform 3 which is one amino acid shorter, compared to isoform 1.

Source sequence(s)

AC083890
NM_001425469.1 → NP_001412398.1 SH2B adapter protein 2 isoform 3

Status: VALIDATED

Description

Transcript Variant: This variant (6) has an alternate exon in the 5' UTR and an alternate splice site in the coding region, compared to variant 1. It encodes isoform 3 which is one amino acid shorter, compared to isoform 1.

Source sequence(s)

AC083890
NM_001425470.1 → NP_001412399.1 SH2B adapter protein 2 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (7) has an alternate exon in the 5' UTR, compared to variant 1. Variants 1, 2, 7 and 8 encode the same isoform (1).

Source sequence(s)

AC083890

UniProtKB/Swiss-Prot

O88936, Q6PG00, Q9JID9
NM_001425471.1 → NP_001412400.1 SH2B adapter protein 2 isoform 1

Status: VALIDATED

Description

Transcript Variant: This variant (8) has an alternate exon in the 5' UTR, compared to variant 1. Variants 1, 2, 7 and 8 encode the same isoform (1).

Source sequence(s)

AC083890

UniProtKB/Swiss-Prot

O88936, Q6PG00, Q9JID9
NM_018825.5 → NP_061295.2 SH2B adapter protein 2 isoform 1

See identical proteins and their annotated locations for NP_061295.2

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1, 2, 7 and 8 encode the same isoform (1).

Source sequence(s)

AC083890

Consensus CDS

CCDS39327.1

UniProtKB/Swiss-Prot

O88936, Q6PG00, Q9JID9

Related

ENSMUSP00000005188.10, ENSMUST00000005188.14

Conserved Domains (3) summary

cd10411
Location:401 → 496

SH2_SH2B2; Src homology 2 (SH2) domain found in SH2B adapter proteins (SH2B1, SH2B2, SH2B3)

cd01231
Location:186 → 301

PH_SH2B_family; SH2B adapter protein 1, 2, and 3 Pleckstrin homology (PH) domain

pfam08916
Location:17 → 73

Phe_ZIP; Phenylalanine zipper