Zp3 zona pellucida glycoprotein 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Zp3provided by MGI
Official Full Name: zona pellucida glycoprotein 3provided by MGI
Primary source: MGI:MGI:99215
See related: Ensembl:ENSMUSG00000004948 AllianceGenome:MGI:99215
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Zp-3
Summary: Enables receptor ligand activity. A structural constituent of egg coat. Involved in several processes, including oogenesis; positive regulation of acrosome reaction; and regulation of gene expression. Acts upstream of or within binding activity of sperm to zona pellucida. Located in collagen-containing extracellular matrix and plasma membrane. Is expressed in ovary and testis. Orthologous to several human genes including ZP3 (zona pellucida glycoprotein 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward ovary adult (RPKM 68.2) See more
Orthologs: human all
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Genomic context

Location:: 5 G2; 5 75.62 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (136008959..136017478)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (135980105..135988624)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy.
Title: Transglutaminase 2 crosslinks zona pellucida glycoprotein 3 to prevent polyspermy.
Novel expression of zona pellucida 3 protein in normal testis; potential functional implications.
Title: Novel expression of zona pellucida 3 protein in normal testis; potential functional implications.
ZP3 regulates Akt phosphorylation, lamin binding to the nuclear membrane via Aipl1, and organization of the actin cytoskeleton via Diaph2.
Title: ZP3 is Required for Germinal Vesicle Breakdown in Mouse Oocyte Meiosis.
Studies indicate that eggs from mZP2-/- and mZP3-/- females lack a zona pellucida (ZP), and the mice are completely infertile due to a severely reduced number of ovulated eggs in their oviducts.
Title: Influence of the zona pellucida of the mouse egg on folliculogenesis and fertility.
Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner.
Title: Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner.
Cytoplasmic tails are necessary to prevent intracellular oligomerization while ensuring incorporation of processed ZP2 and ZP3 into the zona pellucida.
Title: ZP2 and ZP3 cytoplasmic tails prevent premature interactions and ensure incorporation into the zona pellucida.
2.3 angstrom (A) resolution structure of the ZP-N fragment of mouse primary sperm receptor ZP3
Title: Crystal structure of the ZP-N domain of ZP3 reveals the core fold of animal egg coats.
The carboxyl terminal of mZP3 polypeptide backbone interacts with sperm and such interaction plays a significant role in sperm-zona binding, ultimately successful fertilization.
Title: Polypeptide backbone derived from carboxyl terminal of mouse ZP3 inhibits sperm-zona binding.
suggestion of a general mechanism for assembly of ZP domain proteins
Title: A duplicated motif controls assembly of zona pellucida domain proteins.
human and mouse ZP3 proteins are quite similar, and alternative explanations of taxon-specific sperm binding warrant exploration
Title: Mass spectrometry analysis of recombinant human ZP3 expressed in glycosylation-deficient CHO cells.

Submit: New GeneRIF Correction See all GeneRIFs (22)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (2)

General gene information

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Markers

PMC140591P1 (e-PCR)
- UniSTS:270944

Zp3 (e-PCR), detects polymorphism
- UniSTS:525729

Zp3 (e-PCR), detects polymorphism
- UniSTS:144845

Zp3 (e-PCR), detects polymorphism
- UniSTS:144846

NoName (e-PCR)
- UniSTS:489177

X14376 (e-PCR)
- UniSTS:159459

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables acrosin binding	IBA Inferred from Biological aspect of Ancestor more info
enables acrosin binding	ISO Inferred from Sequence Orthology more info
enables carbohydrate binding	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables receptor ligand activity	IDA Inferred from Direct Assay more info	PubMed
enables receptor ligand activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables structural constituent of egg coat	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in binding of sperm to zona pellucida	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within binding of sperm to zona pellucida	IDA Inferred from Direct Assay more info	PubMed
involved_in binding of sperm to zona pellucida	IGI Inferred from Genetic Interaction more info	PubMed
involved_in binding of sperm to zona pellucida	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within binding of sperm to zona pellucida	IPI Inferred from Physical Interaction more info	PubMed
involved_in binding of sperm to zona pellucida	ISO Inferred from Sequence Orthology more info
involved_in blastocyst formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in egg coat formation	IBA Inferred from Biological aspect of Ancestor more info
involved_in egg coat formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in humoral immune response mediated by circulating immunoglobulin	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of binding of sperm to zona pellucida	ISO Inferred from Sequence Orthology more info
involved_in oocyte development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of T cell proliferation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of acrosomal vesicle exocytosis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of acrosome reaction	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of acrosome reaction	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of acrosome reaction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of antral ovarian follicle growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of humoral immune response	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of inflammatory response	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of interleukin-4 production	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of leukocyte migration	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of ovarian follicle development	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of ovarian follicle development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of type II interferon production	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of type IV hypersensitivity	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of signaling receptor activity	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of signaling receptor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in collagen-containing extracellular matrix	IDA Inferred from Direct Assay more info	PubMed
located_in collagen-containing extracellular matrix	ISO Inferred from Sequence Orthology more info
located_in egg coat	ISO Inferred from Sequence Orthology more info
is_active_in extracellular matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular space	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: zona pellucida sperm-binding protein 3

Names: sperm receptor; zona pellucida glycoprotein ZP3; zona pellucida protein C

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.