Thbs1 thrombospondin 1 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Thbs1provided by MGI
Official Full Name: thrombospondin 1provided by MGI
Primary source: MGI:MGI:98737
See related: Ensembl:ENSMUSG00000040152 AllianceGenome:MGI:98737
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: TSP1; TSP-1; tbsp1; Thbs-1
Summary: The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein. This protein is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1. This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
Expression: Broad expression in ovary adult (RPKM 32.7), limb E14.5 (RPKM 32.4) and 21 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 E5; 2 59.34 cM

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (117942357..117957614)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (118111876..118127133)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Thrombospondin-1 promotes mechanical stress-mediated ligamentum flavum hypertrophy through the TGFbeta1/Smad3 signaling pathway.
Title: Thrombospondin-1 promotes mechanical stress-mediated ligamentum flavum hypertrophy through the TGFβ1/Smad3 signaling pathway.
Thrombospondin 1 enhances systemic inflammation and disease severity in acute-on-chronic liver failure.
Title: Thrombospondin 1 enhances systemic inflammation and disease severity in acute-on-chronic liver failure.
Scleral remodeling during myopia development in mice eyes: a potential role of thrombospondin-1.
Title: Scleral remodeling during myopia development in mice eyes: a potential role of thrombospondin-1.
Thrombospondin 1 and Reelin act through Vldlr to regulate cardiac growth and repair.
Title: Thrombospondin 1 and Reelin act through Vldlr to regulate cardiac growth and repair.
Splenocytes with fucosylation deficiency promote T cell proliferation and differentiation through thrombospondin-1 downregulation.
Title: Splenocytes with fucosylation deficiency promote T cell proliferation and differentiation through thrombospondin-1 downregulation.
LRRK2 Gly2019Ser Mutation Promotes ER Stress via Interacting with THBS1/TGF-beta1 in Parkinson's Disease.
Title: LRRK2 Gly2019Ser Mutation Promotes ER Stress via Interacting with THBS1/TGF-β1 in Parkinson's Disease.
CD47 Activation by Thrombospondin-1 in Lymphatic Endothelial Cells Suppresses Lymphangiogenesis and Promotes Atherosclerosis.
Title: CD47 Activation by Thrombospondin-1 in Lymphatic Endothelial Cells Suppresses Lymphangiogenesis and Promotes Atherosclerosis.
O-fucosylation of thrombospondin type I repeats is dispensable for trafficking thrombospondin 1 to platelet secretory granules.
Title: O-fucosylation of thrombospondin type I repeats is dispensable for trafficking thrombospondin 1 to platelet secretory granules.
Thrombospondin 1 missense alleles induce extracellular matrix protein aggregation and TM dysfunction in congenital glaucoma.
Title: Thrombospondin 1 missense alleles induce extracellular matrix protein aggregation and TM dysfunction in congenital glaucoma.
Hypoxia Inhibits Subretinal Inflammation Resolution Thrombospondin-1 Dependently.
Title: Hypoxia Inhibits Subretinal Inflammation Resolution Thrombospondin-1 Dependently.

Submit: New GeneRIF Correction See all GeneRIFs (163)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

M87276 (e-PCR)
- UniSTS:124996

PMC152639P1 (e-PCR)
- UniSTS:271234

RH129576 (e-PCR)
- UniSTS:212877

Thbs1 (e-PCR), detects polymorphism
- UniSTS:547472

D2Mit62 (e-PCR), detects polymorphism
- UniSTS:129457

Thbs1 (e-PCR), detects polymorphism
- UniSTS:516557

Thbs1 (e-PCR), detects polymorphism
- UniSTS:532691

Thbs1 (e-PCR), detects polymorphism
- UniSTS:144618

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables collagen V binding	ISO Inferred from Sequence Orthology more info
enables endopeptidase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables extracellular matrix binding	IDA Inferred from Direct Assay more info	PubMed
enables extracellular matrix structural constituent	RCA inferred from Reviewed Computational Analysis more info	PubMed
enables fibrinogen binding	ISO Inferred from Sequence Orthology more info
enables fibroblast growth factor binding	ISO Inferred from Sequence Orthology more info
enables fibronectin binding	ISO Inferred from Sequence Orthology more info
enables heparin binding	ISO Inferred from Sequence Orthology more info	PubMed
enables integrin binding	ISO Inferred from Sequence Orthology more info
enables laminin binding	ISO Inferred from Sequence Orthology more info
enables low-density lipoprotein particle binding	ISO Inferred from Sequence Orthology more info
enables phosphatidylserine binding	ISO Inferred from Sequence Orthology more info
enables protease binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	ISO Inferred from Sequence Orthology more info
enables transforming growth factor beta binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within angiogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in behavioral response to pain	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within blood vessel morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in cell adhesion	IEA Inferred from Electronic Annotation more info
involved_in cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to nitric oxide	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endocardial cushion development	IGI Inferred from Genetic Interaction more info	PubMed
involved_in engulfment of apoptotic cell	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within growth plate cartilage development	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within growth plate cartilage development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within inflammatory response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in inflammatory response	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within monocyte aggregation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of angiogenesis	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within negative regulation of angiogenesis	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of angiogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info	PubMed
involved_in negative regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of blood vessel endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of blood vessel endothelial cell proliferation involved in sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cGMP-mediated signaling	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell migration involved in sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of cell-matrix adhesion	ISO Inferred from Sequence Orthology more info	PubMed
involved_in negative regulation of cell-matrix adhesion	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of cysteine-type endopeptidase activity involved in apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of dendritic cell antigen processing and presentation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of endothelial cell chemotaxis	ISO Inferred from Sequence Orthology more info	PubMed
involved_in negative regulation of endothelial cell migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of endothelial cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of endothelial cell proliferation	ISO Inferred from Sequence Orthology more info	PubMed
involved_in negative regulation of endothelial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of fibrinolysis	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of fibroblast growth factor receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of interleukin-12 production	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of long-chain fatty acid import across plasma membrane	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of nitric oxide mediated signal transduction	ISO Inferred from Sequence Orthology more info	PubMed
involved_in negative regulation of plasminogen activation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of sprouting angiogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_negative_effect nitric oxide-cGMP-mediated signaling	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within outflow tract morphogenesis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in peptide cross-linking	ISO Inferred from Sequence Orthology more info
acts_upstream_of positive regulation of MAP kinase activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of angiogenesis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of blood coagulation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of blood vessel endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of cell-substrate adhesion	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of chemotaxis	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endothelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endothelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of extrinsic apoptotic signaling pathway via death domain receptors	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of fibroblast migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of macrophage activation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of macrophage chemotaxis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of reactive oxygen species metabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of smooth muscle cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transforming growth factor beta production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transforming growth factor beta receptor signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transforming growth factor beta receptor signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of tumor necrosis factor production	ISO Inferred from Sequence Orthology more info
involved_in response to calcium ion	ISO Inferred from Sequence Orthology more info
involved_in response to endoplasmic reticulum stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to glucose	ISO Inferred from Sequence Orthology more info
involved_in response to magnesium ion	ISO Inferred from Sequence Orthology more info
involved_in response to mechanical stimulus	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to unfolded protein	IEA Inferred from Electronic Annotation more info
involved_in sprouting angiogenesis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cell surface	ISO Inferred from Sequence Orthology more info
located_in collagen-containing extracellular matrix	HDA more info	PubMed
is_active_in collagen-containing extracellular matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in external side of plasma membrane	ISO Inferred from Sequence Orthology more info
located_in extracellular matrix	ISO Inferred from Sequence Orthology more info
located_in extracellular region	IEA Inferred from Electronic Annotation more info
located_in extracellular space	HDA more info	PubMed
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular space	ISO Inferred from Sequence Orthology more info
part_of fibrinogen complex	ISO Inferred from Sequence Orthology more info
located_in platelet alpha granule	ISO Inferred from Sequence Orthology more info
located_in sarcoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in secretory granule	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: thrombospondin-1

Names: glycoprotein G

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001313914.1 → NP_001300843.1 thrombospondin-1 isoform 2 precursor

Status: REVIEWED

Description

Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.

Source sequence(s)

AK163092, AL845495, AL928957, BC050917

UniProtKB/Swiss-Prot

P35441

UniProtKB/TrEMBL

Q3TR40

Conserved Domains (10) summary

smart00209
Location:438 → 490

TSP1; Thrombospondin type 1 repeats

sd00001
Location:845 → 882

TSP3; TSP3 repeat_long [structural motif]

sd00002
Location:787 → 821

TSP3; TSP3 repeat_long [structural motif]

sd00003
Location:691 → 726

TSP3_1C; TSP3 repeat_1C [structural motif]

pfam00090
Location:383 → 428

TSP_1; Thrombospondin type 1 domain

pfam00093
Location:318 → 372

VWC; von Willebrand factor type C domain

pfam02412
Location:846 → 882

TSP_3; Thrombospondin type 3 repeat

pfam05735
Location:972 → 1169

TSP_C; Thrombospondin C-terminal region

pfam12947
Location:650 → 689

EGF_3; EGF domain

cl22861
Location:26 → 221

LamG; Laminin G domain; Laminin G-like domains are usually Ca++ mediated receptors that can have binding sites for steroids, beta1 integrins, heparin, sulfatides, fibulin-1, and alpha-dystroglycans. Proteins that contain LamG domains serve a variety of ...
NM_011580.4 → NP_035710.2 thrombospondin-1 isoform 1 precursor

See identical proteins and their annotated locations for NP_035710.2

Status: REVIEWED

Description

Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).

Source sequence(s)

AK163092, AL845495, AL928957, BC050917

Consensus CDS

CCDS38200.1

UniProtKB/TrEMBL

Q80YQ1, Q8CGB2

Related

ENSMUSP00000044903.9, ENSMUST00000039559.9

Conserved Domains (10) summary

smart00209
Location:439 → 491

TSP1; Thrombospondin type 1 repeats

sd00001
Location:846 → 883

TSP3; TSP3 repeat_long [structural motif]

sd00002
Location:788 → 822

TSP3; TSP3 repeat_long [structural motif]

sd00003
Location:692 → 727

TSP3_1C; TSP3 repeat_1C [structural motif]

pfam00090
Location:384 → 429

TSP_1; Thrombospondin type 1 domain

pfam00093
Location:319 → 373

VWC; von Willebrand factor type C domain

pfam02412
Location:847 → 883

TSP_3; Thrombospondin type 3 repeat

pfam05735
Location:973 → 1170

TSP_C; Thrombospondin C-terminal region

pfam12947
Location:651 → 690

EGF_3; EGF domain

cl22861
Location:26 → 221

LamG; Laminin G domain; Laminin G-like domains are usually Ca++ mediated receptors that can have binding sites for steroids, beta1 integrins, heparin, sulfatides, fibulin-1, and alpha-dystroglycans. Proteins that contain LamG domains serve a variety of ...