Chrm5 cholinergic receptor, muscarinic 5 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Chrm5provided by MGI
Official Full Name: cholinergic receptor, muscarinic 5provided by MGI
Primary source: MGI:MGI:109248
See related: Ensembl:ENSMUSG00000074939 AllianceGenome:MGI:109248
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: m5; M5R; AChR-M5
Summary: Predicted to enable G protein-coupled acetylcholine receptor activity and G protein-coupled serotonin receptor activity. Acts upstream of or within dopamine transport and transmission of nerve impulse. Predicted to be located in postsynaptic membrane. Predicted to be integral component of membrane. Predicted to be active in dendrite and synapse. Predicted to be integral component of plasma membrane. Is expressed in central nervous system and retina. Orthologous to human CHRM5 (cholinergic receptor muscarinic 5). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Low expression observed in reference dataset See more
Orthologs: human all
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Genomic context

Location:: 2 E3; 2 57.02 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (112306055..112365487, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (112478733..112536361, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

This study demonistrated that activation of M5 receptors in the striatum induces an inhibition in dopamine release.
Title: M5 receptor activation produces opposing physiological outcomes in dopamine neurons depending on the receptor's location.
In the absence of VTA M(5) receptors, ventral tegmental area cholinergic agent inputs inhibit locomotion.
Title: M5 muscarinic receptor knockout mice show reduced morphine-induced locomotion but increased locomotion after cholinergic antagonism in the ventral tegmental area.
M2 and M5 muscarinic receptors but not M4 muscarinic receptor or D2 dopamine receptors are needed for male USV production during male-female interactions
Title: Ultrasonic vocalizations induced by sex and amphetamine in M2, M4, M5 muscarinic and D2 dopamine receptor knockout mice.
M5 mAChRs modulate of central dopamine function and the tone of cerebral blood vessels. Review.
Title: Use of M1-M5 muscarinic receptor knockout mice as novel tools to delineate the physiological roles of the muscarinic cholinergic system.
Results suggest that M1 and M5 muscarinic cholinergic receptors are not involved in anti-viral immunity.
Title: Analysis of CD8+ T cell-mediated anti-viral responses in mice with targeted deletions of the M1 or M5 muscarinic cholinergic receptors.
Male M5R-/- mice exhibited a significantly reduced cerebral blood flow (CBF) in the cerebral cortex, hippocampus, basal ganglia, and thalamus. Cortical and hippocampal pyramidal neurons from M5R-/- mice showed neuronal atrophy.
Title: Loss of M5 muscarinic acetylcholine receptors leads to cerebrovascular and neuronal abnormalities and cognitive deficits in mice.
acetylcholine, a potent dilator of most vascular beds, virtually lost the ability to dilate cerebral arteries and arterioles in M5 muscarinic receptor -/- mice, suggesting that endothelial M(5) receptors mediate this activity in wild-type mice--review
Title: Novel insights into M5 muscarinic acetylcholine receptor function by the use of gene targeting technology.
Mutant mice with a deletion in the third intracellular loop of the muscarinic M5 receptor gene exhibited increased drinking compared to wild-type, but feeding was not increased.
Title: Increased drinking in mutant mice with truncated M5 muscarinic receptor genes.
Deletion attenuates morphine reinforcement and withdrawal but not morphine analgesia.
Title: Deletion of the M5 muscarinic acetylcholine receptor attenuates morphine reinforcement and withdrawal but not morphine analgesia.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (5) 1 citation
Endonuclease-mediated (2)

General gene information

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Markers

PMC61174P1 (e-PCR)
- UniSTS:273413

Chrm5 (e-PCR), detects polymorphism
- UniSTS:545912

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables G protein-coupled acetylcholine receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables G protein-coupled acetylcholine receptor activity	ISO Inferred from Sequence Orthology more info
enables G protein-coupled receptor activity	IEA Inferred from Electronic Annotation more info
enables G protein-coupled serotonin receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables extracellularly glycine-gated chloride channel activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger	IBA Inferred from Biological aspect of Ancestor more info
involved_in adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in chemical synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within dopamine transport	IDA Inferred from Direct Assay more info	PubMed
involved_in gastric acid secretion	IEA Inferred from Electronic Annotation more info
involved_in regulation of phosphatidylinositol dephosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within transmission of nerve impulse	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in dendrite	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic membrane	IEA Inferred from Electronic Annotation more info
is_active_in synapse	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: muscarinic acetylcholine receptor M5

Names: muscarinic acetylcholine receptor 5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_205783.3 → NP_991352.2 muscarinic acetylcholine receptor M5

See identical proteins and their annotated locations for NP_991352.2

Status: VALIDATED

Source sequence(s)

AL691423, AL731840

Consensus CDS

CCDS16556.1

UniProtKB/Swiss-Prot

A2AHU3, Q0VBI9, Q920H4

Related

ENSMUSP00000097185.3, ENSMUST00000099589.4

Conserved Domains (3) summary

cd15300
Location:31 → 225

7tmA_mAChR_M5; muscarinic acetylcholine receptor subtype M5, member of the class A family of seven-transmembrane G protein-coupled receptors

cd14964
Location:438 → 463

7tm_GPCRs; TM helix 6 [structural motif]

cl28897
Location:435 → 514

7tm_GPCRs; seven-transmembrane G protein-coupled receptor superfamily