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Mylk3 myosin light chain kinase 3 [ Mus musculus (house mouse) ]

Gene ID: 213435, updated on 5-Mar-2024

Summary

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Official Symbol
Mylk3provided by MGI
Official Full Name
myosin light chain kinase 3provided by MGI
Primary source
MGI:MGI:2443063
See related
Ensembl:ENSMUSG00000031698 AllianceGenome:MGI:2443063
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
MLCK; D830007F02Rik
Summary
Enables calmodulin-dependent protein kinase activity and myosin light chain kinase activity. Involved in protein phosphorylation and sarcomere organization. Located in cytoplasm. Colocalizes with actin cytoskeleton. Is expressed in heart atrium; heart ventricle; inner ear; and liver. Orthologous to human MYLK3 (myosin light chain kinase 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in heart adult (RPKM 54.9), liver E14 (RPKM 12.4) and 2 other tissues See more
Orthologs
human all
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Genomic context

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See Mylk3 in Genome Data Viewer
Location:
8 C3; 8 41.61 cM
Exon count:
15
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 8 NC_000074.7 (86050933..86112969, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 8 NC_000074.6 (85324928..85386340, complement)

Chromosome 8 - NC_000074.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E9120 Neighboring gene origin recognition complex, subunit 6 Neighboring gene predicted gene, 22336 Neighboring gene RIKEN cDNA 4921524J17 gene Neighboring gene STARR-positive B cell enhancer ABC_E3790 Neighboring gene STARR-positive B cell enhancer ABC_E11392 Neighboring gene predicted gene, 53446

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Restoration of Cardiac Myosin Light Chain Kinase Ameliorates Systolic Dysfunction by Reducing Superrelaxed Myosin. Hitsumoto T, et al. Circulation, 2023 Jun 20. PMID 37128901, Free PMC Article
  2. Heterozygous Mylk3 Knockout Mice Partially Recapitulate Human DCM With Heterozygous MYLK3 Mutations. Tougas CL, et al. Front Physiol, 2019. PMID 31244672, Free PMC Article
  3. Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation. Song KH, et al. Sci Rep, 2017 Sep 14. PMID 28912435, Free PMC Article
  4. Heart Failure Induced by Perinatal Ablation of Cardiac Myosin Light Chain Kinase. Islam YF, et al. Front Physiol, 2016. PMID 27833563, Free PMC Article
  5. Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle. Gittings W, et al. Muscle Nerve, 2016 Aug. PMID 26802366

See all (49) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Restoration of Cardiac Myosin Light Chain Kinase Ameliorates Systolic Dysfunction by Reducing Superrelaxed Myosin.
    Title: Restoration of Cardiac Myosin Light Chain Kinase Ameliorates Systolic Dysfunction by Reducing Superrelaxed Myosin.
  2. A temporal Ca(2+) desensitization of myosin light chain kinase in phasic smooth muscles induced by CaMKKbeta/PP2A pathways.
    Title: A temporal Ca(2+) desensitization of myosin light chain kinase in phasic smooth muscles induced by CaMKKβ/PP2A pathways.
  3. Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas Nnt null C57BL/6J mice do not.
    Title: Mylk3 null C57BL/6N mice develop cardiomyopathy, whereas Nnt null C57BL/6J mice do not.
  4. Pharmacological study revealed that lamellipodia formation mediated by arp2/3 and contractility regulated by myosin light chain kinase (MLCK) were responsible for the intriguing turning behavior of T cells climbing the ramp-like structures.
    Title: Turning behaviors of T cells climbing up ramp-like structures are regulated by myosin light chain kinase activity and lamellipodia formation.
  5. Acanthopanax senticosus polysaccharide pretreatment may be associated with inhibition of the NF-kappaB/MLCK pathway and concomitant amelioration of LPS-induced tight junction dysfunction of intestinal epithelium in endotoxemia
    Title: Acanthopanax senticosus polysaccharides-induced intestinal tight junction injury alleviation via inhibition of NF-κB/MLCK pathway in a mouse endotoxemia model.
  6. that MLCK is competing with centrally active ROCK for a limiting pool of monomer with which to drive lamellar filament assembly
    Title: Actin dynamics and competition for myosin monomer govern the sequential amplification of myosin filaments.
  7. This study demonstrated that catchlike property during dynamic performance in mouse hindlimb muscles with (wild-type) and without MLCK(-/-) ) the primary mechanism for PTP (myosin phosphorylation).
    Title: Interaction of posttetanic potentiation and the catchlike property in mouse skeletal muscle.
  8. Ablation of cardiac Mylk3 suggests that Mylk3 plays a pivotal role in the transition from compensated to decompensated hypertrophy via cardiac myocyte atrophy and sarcomeric disorganization.
    Title: Acute heart failure with cardiomyocyte atrophy induced in adult mice by ablation of cardiac myosin light chain kinase.
  9. Data suggest that galectin-3 in hepatocellular carcinoma cells promotes cell migration by inducing RhoA GTPase activity, activating Mlck, up-regulating phosphorylation of MLC2 (myosin light chain 2), and inducing actin rearrangement.
    Title: Galectin 3 regulates HCC cell invasion by RhoA and MLCK activation.
  10. epithelial MLCK-activated brush border fanning by IFN-gamma promotes adherence and internalization of normally noninvasive enteric bacteria
    Title: Commensal bacterial endocytosis in epithelial cells is dependent on myosin light chain kinase-activated brush border fanning by interferon-γ.
Submit: New GeneRIF Correction See all GeneRIFs (27)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)

Pathways from PubChem

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General gene information

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Markers

Homology

Clone Names

  • MGC169607

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
NOT enables calmodulin-dependent protein kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables calmodulin-dependent protein kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables myosin light chain kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables myosin light chain kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables myosin light chain kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in cardiac myofibril assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in cardiac myofibril assembly ISO
Inferred from Sequence Orthology
more info
  
involved_in cellular response to interleukin-1 ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of sarcomere organization ISO
Inferred from Sequence Orthology
more info
  
involved_in protein phosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of vascular permeability involved in acute inflammatory response ISO
Inferred from Sequence Orthology
more info
  
involved_in sarcomere organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in sarcomere organization IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in sarcomere organization ISO
Inferred from Sequence Orthology
more info
  
involved_in sarcomerogenesis ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
colocalizes_with actin cytoskeleton IBA
Inferred from Biological aspect of Ancestor
more info
  
colocalizes_with actin cytoskeleton IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
myosin light chain kinase 3
Names
cardiac MLCK
cardiac-MyBP-C-associated Ca/CaM kinase
cardiac-specific myosin light chain kinase
putative myosin light chain kinase 3
NP_001284541.1
NP_780650.2
XP_006530888.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001297612.1 → NP_001284541.1  myosin light chain kinase 3 isoform 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate first exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1.
    Source sequence(s)
    AK146683, AK165216, BB843262
    Consensus CDS
    CCDS80910.1
    UniProtKB/TrEMBL
    D3Z630
    Related
    ENSMUSP00000113389.2, ENSMUST00000122452.8
    Conserved Domains (2) summary
    smart00220
    Location:361 → 613
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd14192
    Location:353 → 613
    STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3

  2. NM_175441.5 → NP_780650.2  myosin light chain kinase 3 isoform 1

    See identical proteins and their annotated locations for NP_780650.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC134854
    Consensus CDS
    CCDS40422.1
    UniProtKB/Swiss-Prot
    B0LY41, Q3UIZ8, Q3V3V0, Q8BWD1
    UniProtKB/TrEMBL
    B2RQL6
    Related
    ENSMUSP00000034133.8, ENSMUST00000034133.14
    Conserved Domains (2) summary
    smart00220
    Location:494 → 746
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd14192
    Location:486 → 746
    STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000074.7 Reference GRCm39 C57BL/6J

    Range
    86050933..86112969 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006530825.4 → XP_006530888.1  myosin light chain kinase 3 isoform X1

    Conserved Domains (1) summary
    cd14192
    Location:326 → 586
    STKc_MLCK3; Catalytic domain of the Serine/Threonine Kinase, Myosin Light Chain Kinase 3
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q3UIZ8.1 GenPept UniProtKB/Swiss-Prot:Q3UIZ8

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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