Rad21 RAD21 cohesin complex component [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rad21provided by MGI
Official Full Name: RAD21 cohesin complex componentprovided by MGI
Primary source: MGI:MGI:108016
See related: Ensembl:ENSMUSG00000022314 AllianceGenome:MGI:108016
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: SCC1; mHR21; mKIAA0078
Summary: Predicted to enable chromatin binding activity. Involved in negative regulation of G2/M transition of mitotic cell cycle and negative regulation of mitotic metaphase/anaphase transition. Located in chromatin; chromosome, centromeric region; and condensed nuclear chromosome. Part of cohesin complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; integumental system; and sensory organ. Human ortholog(s) of this gene implicated in Cornelia de Lange syndrome 4; hepatocellular carcinoma; and lung non-small cell carcinoma. Orthologous to human RAD21 (RAD21 cohesin complex component). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in CNS E11.5 (RPKM 53.2), liver E14 (RPKM 45.0) and 24 other tissues See more
Orthologs: human all
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Genomic context

Location:: 15 C; 15 19.54 cM

Exon count:: 15

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	15	NC_000081.7 (51826000..51856934, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	15	NC_000081.6 (51962604..51993538, complement)

Chromosome 15 - NC_000081.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cohesin Subunit RAD21 Regulates the Differentiation and Self-Renewal of Hematopoietic Stem and Progenitor Cells.
Title: Cohesin Subunit RAD21 Regulates the Differentiation and Self-Renewal of Hematopoietic Stem and Progenitor Cells.
alpha-Kleisin subunit of cohesin preserves the genome integrity of embryonic stem cells.
Title: α-Kleisin subunit of cohesin preserves the genome integrity of embryonic stem cells.
RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer.
Title: RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer.
Wapl releases Scc1-cohesin and regulates chromosome structure and segregation in mouse oocytes.
Title: Wapl releases Scc1-cohesin and regulates chromosome structure and segregation in mouse oocytes.
A subset of PGP9.5- and HuC/D-IR neuronal cell bodies and nerve fibers in the myenteric plexus of human and mouse small intestine also displayed cytoplasmic RAD21
Title: Expression of RAD21 immunoreactivity in myenteric neurons of the human and mouse small intestine.
The results demonstrate that the zygotic genome folds into loops and domains that critically depend on Scc1-cohesin and that are regulated in size and linear density by Wapl.
Title: A mechanism of cohesin-dependent loop extrusion organizes zygotic genome architecture.
n keratinocytes, the promoter-enhancer anchoring regions in the gene-rich transcriptionally active TADs are enriched for the binding of chromatin architectural proteins CTCF, Rad21 and chromatin remodeler Brg1. In contrast to gene-rich TADs, gene-poor TADs show preferential spatial contacts with each other, do not contain active enhancers and show decreased binding of CTCF, Rad21 and Brg1 in keratinocytes
Title: 5C analysis of the Epidermal Differentiation Complex locus reveals distinct chromatin interaction networks between gene-rich and gene-poor TADs in skin epithelial cells.
the cohesin complex regulates PRC2 targeting to silence Hoxa7 and Hoxa9 and negatively regulate self-renewal. Our studies identify a novel epigenetic mechanism underlying leukemogenesis in AML patients with cohesin mutations.
Title: The cohesin subunit Rad21 is a negative regulator of hematopoietic self-renewal through epigenetic repression of Hoxa7 and Hoxa9.
show that spermatocytes from mice lacking the two meiosis-specific cohesin subunits RAD21L and REC8 were unable to initiate RAD51- but not DMC1-mediated double-strand break repair
Title: Meiotic cohesin complexes are essential for the formation of the axial element in mice.
Knockdown of Rad21 reduces expression of the constitutive, biallelically expressed Pcdhalpha isoforms alphac1 and alphac2.
Title: Role of CCCTC binding factor (CTCF) and cohesin in the generation of single-cell diversity of protocadherin-α gene expression.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (4) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

WI-14191 (e-PCR)
- UniSTS:62229

43.MMHAP79FLG4.seq (e-PCR)
- UniSTS:124511

Rad21 (e-PCR), detects polymorphism
- UniSTS:546973

D49429 (e-PCR)
- UniSTS:124835

Rad21 (e-PCR), detects polymorphism
- UniSTS:144314

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA-binding transcription factor binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	IBA Inferred from Biological aspect of Ancestor more info
enables lncRNA binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within DNA damage response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within DNA repair	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within apoptotic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell cycle	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell division	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within chromatin looping	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within chromosome segregation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within multicellular organism development	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of G2/M transition of mitotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of glial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of interleukin-1 beta production	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of mitotic metaphase/anaphase transition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of tumor necrosis factor production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of interleukin-10 production	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of sister chromatid cohesion	ISO Inferred from Sequence Orthology more info
involved_in protein localization to chromatin	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in replication-born double-strand break repair via sister chromatid exchange	IBA Inferred from Biological aspect of Ancestor more info
involved_in sister chromatid cohesion	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
part_of cohesin complex	IDA Inferred from Direct Assay more info	PubMed
part_of cohesin complex	ISO Inferred from Sequence Orthology more info
located_in condensed nuclear chromosome	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of meiotic cohesin complex	IBA Inferred from Biological aspect of Ancestor more info
located_in midbody	ISO Inferred from Sequence Orthology more info
part_of mitotic cohesin complex	ISO Inferred from Sequence Orthology more info
located_in nuclear matrix	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
located_in nucleoplasm	TAS Traceable Author Statement more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: double-strand-break repair protein rad21 homolog

Names: PW29; RAD21 homolog; SCC1 homolog; pokeweed agglutinin-binding protein 29

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001411716.1 → NP_001398645.1 double-strand-break repair protein rad21 homolog

Status: VALIDATED

Source sequence(s)

AC160550

UniProtKB/Swiss-Prot

P70219, Q3TQ09, Q61550, Q810A8, Q91VB9, Q9DBU4
NM_009009.5 → NP_033035.3 double-strand-break repair protein rad21 homolog

See identical proteins and their annotated locations for NP_033035.3

Status: VALIDATED

Source sequence(s)

AC160550

Consensus CDS

CCDS27463.1

UniProtKB/Swiss-Prot

P70219, Q3TQ09, Q61550, Q810A8, Q91VB9, Q9DBU4

UniProtKB/TrEMBL

Q3TG35

Related

ENSMUSP00000022927.10, ENSMUST00000022927.11

Conserved Domains (2) summary

pfam04824
Location:578 → 632

Rad21_Rec8; Conserved region of Rad21 / Rec8 like protein

pfam04825
Location:1 → 104

Rad21_Rec8_N; N-terminus of Rad21 / Rec8 like protein

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000081.7 Reference GRCm39 C57BL/6J

Range

51826000..51856934 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006520644.1 → XP_006520707.1 double-strand-break repair protein rad21 homolog isoform X1

See identical proteins and their annotated locations for XP_006520707.1

UniProtKB/Swiss-Prot

P70219, Q3TQ09, Q61550, Q810A8, Q91VB9, Q9DBU4

UniProtKB/TrEMBL

Q3TG35

Conserved Domains (2) summary

pfam04824
Location:578 → 632

Rad21_Rec8; Conserved region of Rad21 / Rec8 like protein

pfam04825
Location:1 → 104

Rad21_Rec8_N; N-terminus of Rad21 / Rec8 like protein