Pura purine rich element binding protein A [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Puraprovided by MGI
Official Full Name: purine rich element binding protein Aprovided by MGI
Primary source: MGI:MGI:103079
See related: Ensembl:ENSMUSG00000043991 AllianceGenome:MGI:103079
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: CAGER-1; ssCRE-BP; Pur-alpha; 6330411E07Rik
Summary: Enables several functions, including SMAD binding activity; nucleic acid binding activity; and transcription regulator inhibitor activity. Involved in dendritic transport of messenger ribonucleoprotein complex; negative regulation of DNA-binding transcription factor activity; and negative regulation of transcription, DNA-templated. Acts upstream of or within several processes, including epithelial cell proliferation; lymphocyte proliferation; and positive regulation of cell population proliferation. Located in several cellular components, including dendrite; neuronal cell body; and nucleus. Is active in glutamatergic synapse and postsynapse. Is expressed in several structures, including alimentary system; eye; genitourinary system; heart; and nervous system. Human ortholog(s) of this gene implicated in autosomal dominant non-syndromic intellectual disability 31. Orthologous to human PURA (purine rich element binding protein A). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in cerebellum adult (RPKM 6.9), adrenal adult (RPKM 5.8) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 18; 18 B2

Exon count:: 4

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	18	NC_000084.7 (36413614..36434575)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	18	NC_000084.6 (36280560..36292535)

Chromosome 18 - NC_000084.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DUSP8 induces TGF-beta-stimulated IL-9 transcription and Th9-mediated allergic inflammation by promoting nuclear export of Pur-alpha.
Title: DUSP8 induces TGF-β-stimulated IL-9 transcription and Th9-mediated allergic inflammation by promoting nuclear export of Pur-α.
Analysis of the role of Puralpha in the pathogenesis of Alzheimer's disease based on RNA-seq and ChIP-seq.
Title: Analysis of the role of Purα in the pathogenesis of Alzheimer's disease based on RNA-seq and ChIP-seq.
Puralpha regulates the induction of Znf179 transcription during neuronal differentiation.
Title: Purα regulates the induction of Znf179 transcription during neuronal differentiation.
Quantitative immunohistochemical assays of the vermal region of the cerebellum and CA1-3 regions of the hippocampus revealed reduced numbers of neurons in general, as well as reduced numbers of Pur-alpha+-immunopositive neurons and dendrites in heterozygous Pur-alpha mice, compared to wild-type littermates
Title: Memory deficits, gait ataxia and neuronal loss in the hippocampus and cerebellum in mice that are heterozygous for Pur-alpha.
This study underlines the importance of Puralpha in the proliferation of neuronal precursor cells during postnatal brain development and suggest a role for Puralpha in the regulation of the expression and cellular distribution of dendritic and axonal proteins.
Title: Lack of Pur-alpha alters postnatal brain development and causes megalencephaly.
Data suggest that Puralpha has a role in nucleotide excision repair and the repair of UVC-induced DNA damage.
Title: Role of Purα in the cellular response to ultraviolet-C radiation.
Overexpression of Purbeta, but not Puralpha, significantly downregulated GnRH1 mRNA Levels in transiently transfected GT1-7 cells, suggesting that Purbeta acts as a repressor of GnRH1 gene transcription.
Title: Regulation of gonadotropin-releasing hormone-1 gene transcription by members of the purine-rich element-binding protein family.
analysis of how a bifunctional intronic element regulates the expression of the arginine/lysine transporter Cat-1 via mechanisms involving the purine-rich element binding protein A (Pur alpha)
Title: A bifunctional intronic element regulates the expression of the arginine/lysine transporter Cat-1 via mechanisms involving the purine-rich element binding protein A (Pur alpha).
Expression patterns suggest an accessory role of C9orf10 for Puralpha in a limited brain region in addition to other possible functions that have not yet been determined.
Title: C9orf10 protein, a novel protein component of Puralpha-containing mRNA-protein particles (Puralpha-mRNPs): characterization of developmental and regional expressions in the mouse brain.
Tat stimulates the Rad51 promoter involving both Puralpha-dependent and Puralpha-independent mechanisms. Interaction between Puralpha and Tat may have opposing effects on Rad51 expression.
Title: Role of Puralpha in the modulation of homologous recombination-directed DNA repair by HIV-1 Tat.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (3) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

NoName (e-PCR)
- UniSTS:491273

PURA__5615 (e-PCR)
- UniSTS:463836

Pura (e-PCR), detects polymorphism
- UniSTS:547299

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IBA Inferred from Biological aspect of Ancestor more info
enables DNA-binding transcription factor binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables SMAD binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded telomeric DNA binding	ISO Inferred from Sequence Orthology more info
enables mRNA regulatory element binding translation repressor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables purine-rich negative regulatory element binding	IBA Inferred from Biological aspect of Ancestor more info
enables purine-rich negative regulatory element binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription regulator inhibitor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA unwinding involved in DNA replication	ISO Inferred from Sequence Orthology more info
acts_upstream_of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dendritic transport of messenger ribonucleoprotein complex	IDA Inferred from Direct Assay more info	PubMed
involved_in dendritic transport of messenger ribonucleoprotein complex	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within epithelial cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lymphocyte proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of DNA-binding transcription factor activity	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of DNA-templated transcription	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within nervous system development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynaptic density	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: transcriptional activator protein Pur-alpha

Names: purine-rich single-stranded DNA-binding protein alpha

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_008989.4 → NP_033015.1 transcriptional activator protein Pur-alpha

See identical proteins and their annotated locations for NP_033015.1

Status: VALIDATED

Source sequence(s)

AC131191

Consensus CDS

CCDS29151.1

UniProtKB/Swiss-Prot

P42669

UniProtKB/TrEMBL

Q8C6E9

Related

ENSMUSP00000059404.4, ENSMUST00000051301.6

Conserved Domains (1) summary

pfam04845
Location:57 → 277

PurA; PurA ssDNA and RNA-binding protein

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000084.7 Reference GRCm39 C57BL/6J

Range

36413614..36434575

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006525724.5 → XP_006525787.1 transcriptional activator protein Pur-alpha isoform X1

See identical proteins and their annotated locations for XP_006525787.1

UniProtKB/Swiss-Prot

P42669

UniProtKB/TrEMBL

Q8C6E9

Conserved Domains (1) summary

pfam04845
Location:57 → 277

PurA; PurA ssDNA and RNA-binding protein
XM_011246860.4 → XP_011245162.1 transcriptional activator protein Pur-alpha isoform X1

See identical proteins and their annotated locations for XP_011245162.1

UniProtKB/Swiss-Prot

P42669

UniProtKB/TrEMBL

Q8C6E9

Conserved Domains (1) summary

pfam04845
Location:57 → 277

PurA; PurA ssDNA and RNA-binding protein