Eif2ak2 eukaryotic translation initiation factor 2-alpha kinase 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Eif2ak2provided by MGI
Official Full Name: eukaryotic translation initiation factor 2-alpha kinase 2provided by MGI
Primary source: MGI:MGI:1353449
See related: Ensembl:ENSMUSG00000024079 AllianceGenome:MGI:1353449
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pkr; Tik; Prkr; 2310047A08Rik; 4732414G15Rik
Summary: Enables double-stranded RNA binding activity and protein kinase activity. Involved in several processes, including negative regulation of viral genome replication; positive regulation of intracellular signal transduction; and regulation of hematopoietic stem cell differentiation. Acts upstream of or within cellular protein metabolic process; endoplasmic reticulum unfolded protein response; and negative regulation of apoptotic process. Predicted to be located in nucleus and perinuclear region of cytoplasm. Predicted to be active in cytosol. Is expressed in several structures, including genitourinary system; heart; liver; lung; and spleen. Human ortholog(s) of this gene implicated in Alzheimer's disease; Huntington's disease; Parkinson's disease; and hepatitis B. Orthologous to human EIF2AK2 (eukaryotic translation initiation factor 2 alpha kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in liver E18 (RPKM 2.5), placenta adult (RPKM 2.4) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 17 E3; 17 49.56 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	17	NC_000083.7 (79157933..79190001, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	17	NC_000083.6 (78850504..78882572, complement)

Chromosome 17 - NC_000083.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

ADAR1p150 prevents MDA5 and PKR activation via distinct mechanisms to avert fatal autoinflammation.
Title: ADAR1p150 prevents MDA5 and PKR activation via distinct mechanisms to avert fatal autoinflammation.
A variant NS1 protein from H5N2 avian influenza virus suppresses PKR activation and promotes replication and virulence in mammals.
Title: A variant NS1 protein from H5N2 avian influenza virus suppresses PKR activation and promotes replication and virulence in mammals.
Protein kinase R is an innate immune sensor of proteotoxic stress via accumulation of cytoplasmic IL-24.
Title: Protein kinase R is an innate immune sensor of proteotoxic stress via accumulation of cytoplasmic IL-24.
Mitophagy antagonism by ZIKV reveals Ajuba as a regulator of PINK1 signaling, PKR-dependent inflammation, and viral invasion of tissues.
Title: Mitophagy antagonism by ZIKV reveals Ajuba as a regulator of PINK1 signaling, PKR-dependent inflammation, and viral invasion of tissues.
The PERK/PKR-eIF2alpha Pathway Negatively Regulates Porcine Hemagglutinating Encephalomyelitis Virus Replication by Attenuating Global Protein Translation and Facilitating Stress Granule Formation.
Title: The PERK/PKR-eIF2α Pathway Negatively Regulates Porcine Hemagglutinating Encephalomyelitis Virus Replication by Attenuating Global Protein Translation and Facilitating Stress Granule Formation.
The double-stranded RNA-dependent protein kinase inhibitor alleviates endoplasmic reticulum stress and alleviates sepsis-induced renal injury.
Title: The double-stranded RNA-dependent protein kinase inhibitor alleviates endoplasmic reticulum stress and alleviates sepsis-induced renal injury.
Protein kinase R and the integrated stress response drive immunopathology caused by mutations in the RNA deaminase ADAR1.
Title: Protein kinase R and the integrated stress response drive immunopathology caused by mutations in the RNA deaminase ADAR1.
Double-Stranded RNA Dependent Kinase R Regulates Antibacterial Immunity in Sepsis.
Title: Double-Stranded RNA Dependent Kinase R Regulates Antibacterial Immunity in Sepsis.
The Protein Kinase R Inhibitor C16 Alleviates Sepsis-Induced Acute Kidney Injury Through Modulation of the NF-kappaB and NLR Family Pyrin Domain-Containing 3 (NLPR3) Pyroptosis Signal Pathways.
Title: The Protein Kinase R Inhibitor C16 Alleviates Sepsis-Induced Acute Kidney Injury Through Modulation of the NF-κB and NLR Family Pyrin Domain-Containing 3 (NLPR3) Pyroptosis Signal Pathways.
phosphorylation of eIF2alpha was mediated via protein kinase R (PKR), but further investigation revealed that PKR activation, phosphorylation of eIF2alpha, and translational arrest were uncoupled during viral infection
Title: Mouse Norovirus Infection Arrests Host Cell Translation Uncoupled from the Stress Granule-PKR-eIF2α Axis.

Submit: New GeneRIF Correction See all GeneRIFs (122)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (6)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

STS-Z41731 (e-PCR)
- UniSTS:28930

Eif2ak2 (e-PCR), detects polymorphism
- UniSTS:498740

px-60g6 (e-PCR)
- UniSTS:258437

px-8b4 (e-PCR)
- UniSTS:258464

px-24g4 (e-PCR)
- UniSTS:257458

Eif2ak2 (e-PCR), detects polymorphism
- UniSTS:479156

AI467567 (e-PCR)
- UniSTS:166073

AI747578 (e-PCR)
- UniSTS:166074

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables double-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables double-stranded RNA binding	ISO Inferred from Sequence Orthology more info	PubMed
enables eukaryotic translation initiation factor 2alpha kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables eukaryotic translation initiation factor 2alpha kinase activity	ISO Inferred from Sequence Orthology more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables kinase activity	ISO Inferred from Sequence Orthology more info
enables non-membrane spanning protein tyrosine kinase activity	IEA Inferred from Electronic Annotation more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein tyrosine kinase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in antiviral innate immune response	ISO Inferred from Sequence Orthology more info
involved_in cellular response to amino acid starvation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within defense response to virus	IEA Inferred from Electronic Annotation more info
involved_in eiF2alpha phosphorylation in response to endoplasmic reticulum stress	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within endoplasmic reticulum unfolded protein response	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within immune system process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within innate immune response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of apoptotic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of osteoblast proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of translation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of viral genome replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of viral genome replication	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NF-kappaB transcription factor activity	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of chemokine production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cytokine production	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of non-canonical NF-kappaB signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of stress-activated MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein autophosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein autophosphorylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of NLRP3 inflammasome complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of hematopoietic progenitor cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of hematopoietic stem cell differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of hematopoietic stem cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to interferon-alpha	ISO Inferred from Sequence Orthology more info
involved_in response to lipopolysaccharide	ISO Inferred from Sequence Orthology more info
involved_in response to toxic substance	ISO Inferred from Sequence Orthology more info
involved_in response to virus	ISO Inferred from Sequence Orthology more info
involved_in response to vitamin E	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within translation	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: interferon-induced, double-stranded RNA-activated protein kinase

Names: IFN- type I-induced and dsRNA-activated kinase; IFN-induced and double-stranded RNA-activated kinase; P1/eIF-2A protein kinase; T-cell viral integration site; dsRNA-activated kinase; eIF-2 alpha; eIF-2A protein kinase 2; interferon-inducible RNA-dependent protein kinase; p68 kinase; protein kinase R; protein kinase RNA-activated; protein kinase, interferon-inducible double stranded RNA dependent; serine/threonine-protein kinase TIK; tyrosine-protein kinase EIF2AK2

NP_035293.1

EC 2.7.10.2

EC 2.7.11.1

XP_006523926.1

EC 2.7.10.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011163.4 → NP_035293.1 interferon-induced, double-stranded RNA-activated protein kinase

See identical proteins and their annotated locations for NP_035293.1

Status: VALIDATED

Source sequence(s)

AK028602, BU756642

Consensus CDS

CCDS28980.1

UniProtKB/Swiss-Prot

Q03963, Q61742, Q62026

UniProtKB/TrEMBL

Q91YN2

Related

ENSMUSP00000024884.5, ENSMUST00000024884.5

Conserved Domains (3) summary

smart00220
Location:242 → 445

S_TKc; Serine/Threonine protein kinases, catalytic domain

smart00358
Location:9 → 74

DSRM; Double-stranded RNA binding motif

cl21453
Location:235 → 500

PKc_like; Protein Kinases, catalytic domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000083.7 Reference GRCm39 C57BL/6J

Range

79157933..79190001 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006523863.5 → XP_006523926.1 interferon-induced, double-stranded RNA-activated protein kinase isoform X1

UniProtKB/TrEMBL

Q91YN2

Conserved Domains (3) summary

cd19903
Location:21 → 88

DSRM_EIF2AK2_rpt1; first double-stranded RNA binding motif of eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2) and similar proteins

cd19904
Location:108 → 175

DSRM_EIF2AK2_rpt2; second double-stranded RNA binding motif of eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2) and similar proteins

cl21453
Location:249 → 514

PKc_like; Protein Kinases, catalytic domain