Prkar2b protein kinase, cAMP dependent regulatory, type II beta [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Prkar2bprovided by MGI
Official Full Name: protein kinase, cAMP dependent regulatory, type II betaprovided by MGI
Primary source: MGI:MGI:97760
See related: Ensembl:ENSMUSG00000002997 AllianceGenome:MGI:97760
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Pkarb2; RII(beta); PKARIIbeta
Summary: Enables cAMP-dependent protein kinase regulator activity and protein kinase binding activity. Involved in modulation of chemical synaptic transmission. Acts upstream of or within fatty acid metabolic process and learning. Located in several cellular components, including ciliary base; dendrite; and glutamatergic synapse. Part of cAMP-dependent protein kinase complex. Is expressed in several structures, including central nervous system; liver; primary palate; retina; and testis. Orthologous to human PRKAR2B (protein kinase cAMP-dependent type II regulatory subunit beta). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Broad expression in ovary adult (RPKM 61.4), adrenal adult (RPKM 55.1) and 19 other tissues See more
Orthologs: human all
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Genomic context

Location:: 12 A3; 12 13.74 cM

Exon count:: 14

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	12	NC_000078.7 (32008468..32111639, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	12	NC_000078.6 (31958450..32061648, complement)

Chromosome 12 - NC_000078.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RIIbeta-PKA in GABAergic Neurons of Dorsal Median Hypothalamus Governs White Adipose Browning.
Title: RIIβ-PKA in GABAergic Neurons of Dorsal Median Hypothalamus Governs White Adipose Browning.
PKA-RIIbeta autophosphorylation modulates PKA activity and seizure phenotypes in mice.
Title: PKA-RIIβ autophosphorylation modulates PKA activity and seizure phenotypes in mice.
RNAi-mediated Prkar2b suppression resulted in MI-stage arrest during oocyte development, and these oocytes exhibited abnormal spindle formation and chromosome aggregation.
Title: Knockdown of PRKAR2B Results in the Failure of Oocyte Maturation.
deficiency induces brownlike adipocytes in inguinal white adipose tissue, increases expression of uncoupling protein1 and other thermogenic genes
Title: PKA-RIIB Deficiency Induces Brown Fatlike Adipocytes in Inguinal WAT and Promotes Energy Expenditure in Male FVB/NJ Mice.
Data (including data from studies using knockout mice) suggest that S100A1 (S-100 calcium-binding protein A1, alpha chain) is involved in protein kinase A- (RIIalpha and RIIbeta)-dependent signaling resulting in nuclear redistribution/influx of HDAC4 (histone deacetylase 4) in skeletal muscle fibers.
Title: The Activation of Protein Kinase A by the Calcium-Binding Protein S100A1 Is Independent of Cyclic AMP.
RIIbeta-PKA modulates the duration of leptin receptor signalling and therefore the magnitude of the catabolic response to leptin.
Title: Hypothalamic PKA regulates leptin sensitivity and adiposity.
Release of pRIIbeta in the presence of cAMP is reduced by calcium, whereas autophosphorylation at the phosphorylation site inhibits holoenzyme reassociation with the catalytic subunit.
Title: Single Turnover Autophosphorylation Cycle of the PKA RIIβ Holoenzyme.
RIIBeta protein kinase A knockout was protective against induced seizure susceptibility.
Title: Altered GABAA receptor expression and seizure threshold following acute ethanol challenge in mice lacking the RIIβ subunit of PKA.
Deficiency of the RIIbeta subunit of PKA affects locomotor activity and energy homeostasis in distinct neuronal populations.
Title: Deficiency of the RIIβ subunit of PKA affects locomotor activity and energy homeostasis in distinct neuronal populations.
study describes the 2.3 angstrom structure of full-length tetrameric RIIbeta(2):C(2) holoenzyme
Title: Structure and allostery of the PKA RIIβ tetrameric holoenzyme.

Submit: New GeneRIF Correction See all GeneRIFs (17)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AW061005 (e-PCR)
- UniSTS:180379

AI451071 (e-PCR)
- UniSTS:160197

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables cAMP binding	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP binding	ISO Inferred from Sequence Orthology more info
enables cAMP-dependent protein kinase inhibitor activity	IBA Inferred from Biological aspect of Ancestor more info
enables cAMP-dependent protein kinase inhibitor activity	ISO Inferred from Sequence Orthology more info
enables cAMP-dependent protein kinase regulator activity	IDA Inferred from Direct Assay more info	PubMed
enables cAMP-dependent protein kinase regulator activity	ISO Inferred from Sequence Orthology more info
enables nucleotide binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein domain specific binding	ISO Inferred from Sequence Orthology more info
enables protein kinase A catalytic subunit binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase A catalytic subunit binding	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin protein ligase binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within fatty acid metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within learning	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cAMP-dependent protein kinase activity	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of cAMP-dependent protein kinase activity	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein phosphorylation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of cAMP-dependent protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of cAMP-dependent protein kinase complex	IDA Inferred from Direct Assay more info	PubMed
part_of cAMP-dependent protein kinase complex	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in ciliary base	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in dendrite	ISO Inferred from Sequence Orthology more info
located_in dendritic shaft	IDA Inferred from Direct Assay more info	PubMed
located_in dendritic spine	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
located_in perinuclear region of cytoplasm	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in postsynapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: cAMP-dependent protein kinase type II-beta regulatory subunit

NP_001351336.1

EC 2.7.11.1

NP_001351337.1

EC 2.7.11.1

NP_001409252.1

EC 2.7.11.1

NP_035288.2

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001364407.1 → NP_001351336.1 cAMP-dependent protein kinase type II-beta regulatory subunit isoform 1

Status: VALIDATED

Source sequence(s)

CT010463

Consensus CDS

CCDS25869.1

UniProtKB/Swiss-Prot

B1B199, P31324, Q3UTZ1, Q80ZM4, Q8BRZ7

Related

ENSMUSP00000003079.6, ENSMUST00000003079.12

Conserved Domains (2) summary

cd12104
Location:3 → 43

DD_RIIbeta_PKA; Dimerization/Docking domain of the Type II beta Regulatory subunit of cAMP-dependent protein kinase

cd00038
Location:274 → 394

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...
NM_001364408.1 → NP_001351337.1 cAMP-dependent protein kinase type II-beta regulatory subunit isoform 2

Status: VALIDATED

Source sequence(s)

CT010463

Consensus CDS

CCDS88324.1

UniProtKB/TrEMBL

H3BK84, Q3V2X9

Related

ENSMUSP00000135290.2, ENSMUST00000146865.2

Conserved Domains (1) summary

cd00038
Location:114 → 234

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...
NM_001422323.1 → NP_001409252.1 cAMP-dependent protein kinase type II-beta regulatory subunit isoform 2

Status: VALIDATED

Source sequence(s)

CT010463

UniProtKB/TrEMBL

H3BK84
NM_011158.4 → NP_035288.2 cAMP-dependent protein kinase type II-beta regulatory subunit isoform 1

See identical proteins and their annotated locations for NP_035288.2

Status: VALIDATED

Source sequence(s)

AK135621, AK138963, CJ238090, CT010463

Consensus CDS

CCDS25869.1

UniProtKB/Swiss-Prot

B1B199, P31324, Q3UTZ1, Q80ZM4, Q8BRZ7

Related

ENSMUSP00000039797.10, ENSMUST00000036497.16

Conserved Domains (2) summary

cd12104
Location:3 → 43

DD_RIIbeta_PKA; Dimerization/Docking domain of the Type II beta Regulatory subunit of cAMP-dependent protein kinase

cd00038
Location:274 → 394

CAP_ED; effector domain of the CAP family of transcription factors; members include CAP (or cAMP receptor protein (CRP)), which binds cAMP, FNR (fumarate and nitrate reduction), which uses an iron-sulfur cluster to sense oxygen) and CooA, a heme containing CO ...