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Pola2 polymerase (DNA directed), alpha 2 [ Mus musculus (house mouse) ]

Gene ID: 18969, updated on 11-Apr-2024

Summary

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Official Symbol
Pola2provided by MGI
Official Full Name
polymerase (DNA directed), alpha 2provided by MGI
Primary source
MGI:MGI:99690
See related
Ensembl:ENSMUSG00000024833 AllianceGenome:MGI:99690
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary
Predicted to enable DNA-directed DNA polymerase activity. Involved in DNA replication, synthesis of RNA primer. Acts upstream of or within DNA replication and protein import into nucleus. Located in nucleus. Part of alpha DNA polymerase:primase complex. Is expressed in several structures, including alimentary system; brain; genitourinary system; integumental system; and sensory organ. Orthologous to human POLA2 (DNA polymerase alpha 2, accessory subunit). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in liver E14.5 (RPKM 23.1), liver E14 (RPKM 19.9) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Pola2 in Genome Data Viewer
Location:
19 A; 19 4.34 cM
Exon count:
21
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 19 NC_000085.7 (5990574..6014266, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 19 NC_000085.6 (5940543..5964238, complement)

Chromosome 19 - NC_000085.7Genomic Context describing neighboring genes Neighboring gene STARR-positive B cell enhancer ABC_E7628 Neighboring gene predicted gene, 42067 Neighboring gene CDC42 effector protein 2 Neighboring gene STARR-positive B cell enhancer ABC_E8731 Neighboring gene solute carrier family 22 (organic anion transporter), member 20 Neighboring gene predicted gene, 31719 Neighboring gene calpain 1 Neighboring gene predicted gene 10814

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Molecular architecture of the mouse DNA polymerase alpha-primase complex. Mizuno T, et al. Mol Cell Biol, 1999 Nov. PMID 10523676, Free PMC Article
  2. Molecular cloning of the cDNAs for the four subunits of mouse DNA polymerase alpha-primase complex and their gene expression during cell proliferation and the cell cycle. Miyazawa H, et al. J Biol Chem, 1993 Apr 15. PMID 8463324
  3. Role of the second-largest subunit of DNA polymerase alpha in the interaction between the catalytic subunit and hyperphosphorylated retinoblastoma protein in late S phase. Takemura M, et al. Biochim Biophys Acta, 2006 Sep. PMID 16935576
  4. The second-largest subunit of the mouse DNA polymerase alpha-primase complex facilitates both production and nuclear translocation of the catalytic subunit of DNA polymerase alpha. Mizuno T, et al. Mol Cell Biol, 1998 Jun. PMID 9584195, Free PMC Article
  5. DNA replication in vitro by recombinant DNA-polymerase-alpha-primase. Stadlbauer F, et al. Eur J Biochem, 1994 Jun 15. PMID 8026492

See all (30) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. nonphosphorylated p68 inhibited the stimulation of pol-alpha activity by hyperphosphorylated retinoblastoma protein, suggesting that p68 might impede the association of ppRb with p180 [Dna polymerase alpha p68]
    Title: Role of the second-largest subunit of DNA polymerase alpha in the interaction between the catalytic subunit and hyperphosphorylated retinoblastoma protein in late S phase.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (2) 
  • Endonuclease-mediated (2) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA-directed DNA polymerase activity ISO
Inferred from Sequence Orthology
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
acts_upstream_of_or_within DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA replication ISO
Inferred from Sequence Orthology
more info
  
involved_in DNA replication initiation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA replication initiation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA replication initiation ISO
Inferred from Sequence Orthology
more info
  
involved_in DNA replication, synthesis of primer IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA replication, synthesis of primer ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within protein import into nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of alpha DNA polymerase:primase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of alpha DNA polymerase:primase complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of alpha DNA polymerase:primase complex IPI
Inferred from Physical Interaction
more info
 PubMed 
part_of alpha DNA polymerase:primase complex ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
DNA polymerase alpha subunit B
Names
DNA polymerase alpha 2, 68 kDa
DNA polymerase alpha 70 kDa subunit

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001164057.2 → NP_001157529.1  DNA polymerase alpha subunit B isoform 2

    See identical proteins and their annotated locations for NP_001157529.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.
    Source sequence(s)
    AC131692
    Consensus CDS
    CCDS50362.1
    UniProtKB/TrEMBL
    Q3UZI8, Q8C2T6
    Related
    ENSMUSP00000128866.2, ENSMUST00000165143.3
    Conserved Domains (2) summary
    pfam04042
    Location:343 → 549
    DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
    pfam08418
    Location:20 → 238
    Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

  2. NM_008893.4 → NP_032919.2  DNA polymerase alpha subunit B isoform 1

    See identical proteins and their annotated locations for NP_032919.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC131692
    Consensus CDS
    CCDS29485.1
    UniProtKB/Swiss-Prot
    P33611, Q8VDR3
    UniProtKB/TrEMBL
    Q3UZI8
    Related
    ENSMUSP00000025752.8, ENSMUST00000025752.15
    Conserved Domains (2) summary
    pfam04042
    Location:343 → 549
    DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
    pfam08418
    Location:20 → 238
    Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

RNA

  1. NR_177189.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC131692

  2. NR_177190.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AC131692

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000085.7 Reference GRCm39 C57BL/6J

    Range
    5990574..6014266 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006531681.4 → XP_006531744.1  DNA polymerase alpha subunit B isoform X2

    UniProtKB/TrEMBL
    Q3UZI8
    Conserved Domains (2) summary
    pfam04042
    Location:343 → 592
    DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
    pfam08418
    Location:21 → 238
    Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

  2. XM_011248610.3 → XP_011246912.1  DNA polymerase alpha subunit B isoform X5

    UniProtKB/TrEMBL
    Q3UZI8
    Conserved Domains (2) summary
    pfam04042
    Location:358 → 564
    DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
    pfam08418
    Location:21 → 253
    Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

  3. XM_011248607.3 → XP_011246909.1  DNA polymerase alpha subunit B isoform X1

    UniProtKB/TrEMBL
    Q3UZI8
    Conserved Domains (2) summary
    pfam04042
    Location:358 → 607
    DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
    pfam08418
    Location:21 → 253
    Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

  4. XM_006531683.5 → XP_006531746.1  DNA polymerase alpha subunit B isoform X6

    UniProtKB/TrEMBL
    Q3UZI8
    Conserved Domains (2) summary
    pfam04042
    Location:303 → 552
    DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
    pfam08418
    Location:15 → 198
    Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

  5. XM_011248609.3 → XP_011246911.1  DNA polymerase alpha subunit B isoform X4

    UniProtKB/TrEMBL
    Q3UZI8
    Conserved Domains (2) summary
    pfam04042
    Location:318 → 567
    DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
    pfam08418
    Location:15 → 213
    Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

  6. XM_006531684.5 → XP_006531747.1  DNA polymerase alpha subunit B isoform X10

    Conserved Domains (1) summary
    pfam04042
    Location:40 → 289
    DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B

  7. XM_030250774.2 → XP_030106634.1  DNA polymerase alpha subunit B isoform X7

    UniProtKB/TrEMBL
    Q3UZI8
    Conserved Domains (2) summary
    pfam04042
    Location:358 → 564
    DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
    pfam08418
    Location:21 → 253
    Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

  8. XM_011248608.4 → XP_011246910.1  DNA polymerase alpha subunit B isoform X3

    UniProtKB/TrEMBL
    Q3UZI8
    Conserved Domains (2) summary
    pfam04042
    Location:358 → 607
    DNA_pol_E_B; DNA polymerase alpha/epsilon subunit B
    pfam08418
    Location:21 → 253
    Pol_alpha_B_N; DNA polymerase alpha subunit B N-terminal

RNA

  1. XR_004940121.1 RNA Sequence

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P33611.2 GenPept UniProtKB/Swiss-Prot:P33611

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