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Pfkfb1 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1 [ Mus musculus (house mouse) ]

Gene ID: 18639, updated on 5-Mar-2024

Summary

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Official Symbol
Pfkfb1provided by MGI
Official Full Name
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1provided by MGI
Primary source
MGI:MGI:107816
See related
Ensembl:ENSMUSG00000025271 AllianceGenome:MGI:107816
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
PFK/FBPase 1
Summary
Predicted to enable several functions, including 6-phosphofructo-2-kinase activity; anion binding activity; and fructose-2,6-bisphosphate 2-phosphatase activity. Predicted to be involved in carbohydrate phosphorylation; fructose 2,6-bisphosphate metabolic process; and positive regulation of glucokinase activity. Predicted to act upstream of or within phosphorylation. Predicted to be part of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex. Predicted to be active in cytosol. Orthologous to human PFKFB1 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 1). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in liver E18 (RPKM 56.0), mammary gland adult (RPKM 15.0) and 5 other tissues See more
Orthologs
human all
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Genomic context

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Location:
X F3; X 68.46 cM
Exon count:
21
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) X NC_000086.8 (149370441..149426874)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) X NC_000086.7 (150587445..150643878)

Chromosome X - NC_000086.8Genomic Context describing neighboring genes Neighboring gene apurinic/apyrimidinic endonuclease 2 Neighboring gene microRNA 3620 Neighboring gene Alas2R1 erythroid cis-regulatory module Neighboring gene aminolevulinic acid synthase 2, erythroid Neighboring gene Alas2R3 erythroid cis-regulatory module Neighboring gene trophinin Neighboring gene voltage-dependent anion channel 1 pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice using in vivo (13)C NMR measurements of hepatic carbohydrate metabolism. Choi IY, et al. Eur J Biochem, 2002 Sep. PMID 12230553
  2. Inhibition of fructose-1,6-bisphosphatase by fructose 2,6-biphosphate. Van Schaftingen E, et al. Proc Natl Acad Sci U S A, 1981 May. PMID 6265919, Free PMC Article
  3. Cardiac expression of kinase-deficient 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase inhibits glycolysis, promotes hypertrophy, impairs myocyte function, and reduces insulin sensitivity. Donthi RV, et al. J Biol Chem, 2004 Nov 12. PMID 15331593
  4. Complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a cDNA clone. Darville MI, et al. FEBS Lett, 1987 Nov 30. PMID 2856848
  5. Rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase. Properties of phospho- and dephospho- forms and of two mutants in which Ser32 has been changed by site-directed mutagenesis. Kurland IJ, et al. J Biol Chem, 1992 Mar 5. PMID 1339450

See all (25) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that cardiac overexpression of kinase-deficient PFK-2 reduces cardiac glycolysis that produced negative consequences to the heart including hypertrophy, fibrosis, and reduced cardiomyocyte function.
    Title: Cardiac expression of kinase-deficient 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase inhibits glycolysis, promotes hypertrophy, impairs myocyte function, and reduces insulin sensitivity.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Targeted (2) 
  • Endonuclease-mediated (2) 

Pathways from PubChem

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General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables 6-phosphofructo-2-kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 6-phosphofructo-2-kinase activity ISO
Inferred from Sequence Orthology
more info
 PubMed 
enables ATP binding ISO
Inferred from Sequence Orthology
more info
  
enables catalytic activity IEA
Inferred from Electronic Annotation
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables fructose-2,6-bisphosphate 2-phosphatase activity IGI
Inferred from Genetic Interaction
more info
 PubMed 
enables fructose-2,6-bisphosphate 2-phosphatase activity ISO
Inferred from Sequence Orthology
more info
 PubMed 
enables fructose-6-phosphate binding ISO
Inferred from Sequence Orthology
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables kinase binding ISO
Inferred from Sequence Orthology
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in carbohydrate phosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in fructose 2,6-bisphosphate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in fructose 2,6-bisphosphate metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in fructose metabolic process IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in negative regulation of glycolytic process through fructose-6-phosphate IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in negative regulation of glycolytic process through fructose-6-phosphate IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of gluconeogenesis ISO
Inferred from Sequence Orthology
more info
 PubMed 
involved_in positive regulation of glycolytic process through fructose-6-phosphate ISO
Inferred from Sequence Orthology
more info
 PubMed 
Component Evidence Code Pubs
part_of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytosol IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cytosol ISO
Inferred from Sequence Orthology
more info
 PubMed 

General protein information

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Preferred Names
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1
Names
6PF-2-K/Fru-2,6-P2ase liver isozyme
PFK-2/FBPase-2 gene A
NP_001361099.1
NP_001361100.1
NP_001361101.1
NP_001361102.1
NP_001361104.1
NP_001398692.1
NP_001398693.1
NP_032850.1
XP_006528819.1
XP_036017742.1
XP_036017743.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001374170.1 → NP_001361099.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AL672150
    UniProtKB/TrEMBL
    A2AFM9
    Conserved Domains (2) summary
    pfam00300
    Location:231 → 417
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:7 → 228
    6PF2K; 6-phosphofructo-2-kinase

  2. NM_001374171.1 → NP_001361100.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AL672150
    Consensus CDS
    CCDS30464.1
    UniProtKB/TrEMBL
    A2AFM9
    Conserved Domains (2) summary
    pfam00300
    Location:231 → 417
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:7 → 228
    6PF2K; 6-phosphofructo-2-kinase

  3. NM_001374172.1 → NP_001361101.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AL672150
    Consensus CDS
    CCDS30464.1
    UniProtKB/TrEMBL
    A2AFM9
    Conserved Domains (2) summary
    pfam00300
    Location:231 → 417
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:7 → 228
    6PF2K; 6-phosphofructo-2-kinase

  4. NM_001374173.1 → NP_001361102.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AL672150
    Consensus CDS
    CCDS90763.1
    UniProtKB/Swiss-Prot
    A2AFN0, P70266, Q3V180
    Related
    ENSMUSP00000108333.3, ENSMUST00000112713.3
    Conserved Domains (2) summary
    pfam01591
    Location:33 → 251
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:254 → 437
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  5. NM_001374175.1 → NP_001361104.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AL672150
    UniProtKB/TrEMBL
    A2AFM8
    Conserved Domains (1) summary
    pfam01591
    Location:33 → 210
    6PF2K; 6-phosphofructo-2-kinase

  6. NM_001411763.1 → NP_001398692.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AL672150
    UniProtKB/TrEMBL
    A2AFM9

  7. NM_001411764.1 → NP_001398693.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 4

    Status: VALIDATED

    Source sequence(s)
    AL672150

  8. NM_008824.4 → NP_032850.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform 2

    See identical proteins and their annotated locations for NP_032850.1

    Status: VALIDATED

    Source sequence(s)
    AL672150
    Consensus CDS
    CCDS30464.1
    UniProtKB/TrEMBL
    A2AFM9
    Related
    ENSMUSP00000079692.5, ENSMUST00000080884.11
    Conserved Domains (2) summary
    pfam00300
    Location:231 → 417
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:7 → 228
    6PF2K; 6-phosphofructo-2-kinase

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000086.8 Reference GRCm39 C57BL/6J

    Range
    149370441..149426874
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_036161850.1 → XP_036017743.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X2

    UniProtKB/TrEMBL
    A2AFM9
    Conserved Domains (2) summary
    pfam00300
    Location:231 → 417
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:7 → 228
    6PF2K; 6-phosphofructo-2-kinase

  2. XM_006528756.4 → XP_006528819.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X4

    Conserved Domains (2) summary
    pfam01591
    Location:33 → 251
    6PF2K; 6-phosphofructo-2-kinase
    pfam00300
    Location:254 → 366
    His_Phos_1; Histidine phosphatase superfamily (branch 1)

  3. XM_036161849.1 → XP_036017742.1  6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 1 isoform X1

    Conserved Domains (2) summary
    pfam00300
    Location:235 → 421
    His_Phos_1; Histidine phosphatase superfamily (branch 1)
    pfam01591
    Location:30 → 232
    6PF2K; 6-phosphofructo-2-kinase
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P70266.2 GenPept UniProtKB/Swiss-Prot:P70266

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