Nck2 non-catalytic region of tyrosine kinase adaptor protein 2 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Nck2provided by MGI
Official Full Name: non-catalytic region of tyrosine kinase adaptor protein 2provided by MGI
Primary source: MGI:MGI:1306821
See related: Ensembl:ENSMUSG00000066877 AllianceGenome:MGI:1306821
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Grb4; Nck-2; NCKbeta; 4833426I10Rik
Summary: Enables scaffold protein binding activity. Involved in regulation of cellular macromolecule biosynthetic process and regulation of response to endoplasmic reticulum stress. Acts upstream of or within several processes, including actin filament organization; ephrin receptor signaling pathway; and lamellipodium assembly. Located in vesicle membrane. Is expressed in several structures, including central nervous system; chordamesoderm; gut endoderm; hemolymphoid system gland; and paraxial mesenchyme. Orthologous to human NCK2 (NCK adaptor protein 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in thymus adult (RPKM 33.3), colon adult (RPKM 26.2) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 1 C1.1; 1 23.18 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	1	NC_000067.7 (43484643..43609678)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	1	NC_000067.6 (43445483..43570518)

Chromosome 1 - NC_000067.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Distinct Requirements for Adaptor Proteins NCK1 and NCK2 in Mammary Gland Development.
Title: Distinct Requirements for Adaptor Proteins NCK1 and NCK2 in Mammary Gland Development.
Authors propose that Nck2 should be further investigated as a regulator of the reliance of white adipose tissue on hyperplasia versus hypertrophy during adipose tissue expansion, and hence, as a potential novel molecular target in obesity.
Title: Nck2, an unexpected regulator of adipogenesis.
Findings reveal that Nck2 is a novel regulator of adiposity and suggest that Nck2 is important in limiting WAT expansion and dysfunction in mice and humans.
Title: Nck2 Deficiency in Mice Results in Increased Adiposity Associated With Adipocyte Hypertrophy and Enhanced Adipogenesis.
Inactivation of NCK2 (and NCK1) inhibits endothelial cell polarity.
Title: Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization.
These results reveal that Nck regulates preosteoblastic/osteoblastic migration and bone mass.
Title: Nck influences preosteoblastic/osteoblastic migration and bone mass.
we show that the non-catalytic region of tyrosine kinase adaptor (NCK) proteins 1 and 2 are distributed in the developing spinal cord
Title: NCK is critical for the development of deleted in colorectal cancer (DCC) sensitive spinal circuits.
Mouse embryos lacking endothelial Nck1/2 expression develop extensive angiogenic defects that result in lethality at about embryonic day 10.
Title: Requisite role for Nck adaptors in cardiovascular development, endothelial-to-mesenchymal transition, and directed cell migration.
Data show that only Nck2 is required for the Slit1-induced changes in cortical neuron morphology in vitro.
Title: The adaptor protein Nck2 mediates Slit1-induced changes in cortical neuron morphology.
These results revealed an essential role for HSF4-mediated SKAP2 expression in the regulation of actin reorganization during lens differentiation.
Title: SKAP2, a novel target of HSF4b, associates with NCK2/F-actin at membrane ruffles and regulates actin reorganization in lens cell.
The Cas utilizes Nck2 to activate Cdc42 and induce cell polarization in response to wound healing.
Title: Cas utilizes Nck2 to activate Cdc42 and regulate cell polarization during cell migration in response to wound healing.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (3) 1 citation
Endonuclease-mediated (1)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

AF043260 (e-PCR)
- UniSTS:207153

Nck2 (e-PCR), detects polymorphism
- UniSTS:527436

D1Mit248 (e-PCR), detects polymorphism
- UniSTS:128591

D1Mit375 (e-PCR), detects polymorphism
- UniSTS:128735

06.MMHAP25FRB9.seq (e-PCR)
- UniSTS:122852

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables phosphotyrosine residue binding	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables receptor tyrosine kinase binding	IBA Inferred from Biological aspect of Ancestor more info
enables scaffold protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables signaling adaptor activity	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell migration	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within dendritic spine development	ISO Inferred from Sequence Orthology more info	PubMed
involved_in ephrin receptor signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within ephrin receptor signaling pathway	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within immunological synapse formation	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within lamellipodium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of PERK-mediated unfolded protein response	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of PERK-mediated unfolded protein response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of T cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of actin filament polymerization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of peptidyl-serine dephosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of translation in response to endoplasmic reticulum stress	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of translation in response to endoplasmic reticulum stress	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in response to endoplasmic reticulum stress	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in postsynaptic density	ISO Inferred from Sequence Orthology more info	PubMed
located_in synapse	ISO Inferred from Sequence Orthology more info	PubMed
located_in vesicle membrane	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: cytoplasmic protein NCK2

Names: NCK adaptor protein 2; SH2/SH3 adaptor protein NCK-beta; growth factor receptor-bound protein 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010879.3 → NP_035009.3 cytoplasmic protein NCK2

See identical proteins and their annotated locations for NP_035009.3

Status: VALIDATED

Source sequence(s)

AK014949, AK051665, BQ176012

Consensus CDS

CCDS14924.1

UniProtKB/Swiss-Prot

O55033

UniProtKB/TrEMBL

Q8BQ28

Related

ENSMUSP00000083611.6, ENSMUST00000086421.9

Conserved Domains (4) summary

cd10409
Location:283 → 380

SH2_Nck2; Src homology 2 (SH2) domain found in Nck

cd11899
Location:2 → 59

SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein

cd11902
Location:114 → 168

SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein

cd11903
Location:198 → 256

SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000067.7 Reference GRCm39 C57BL/6J

Range

43484643..43609678

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_030252054.1 → XP_030107914.1 cytoplasmic protein NCK2 isoform X1

UniProtKB/Swiss-Prot

O55033

UniProtKB/TrEMBL

Q8BQ28

Conserved Domains (4) summary

cd10409
Location:283 → 380

SH2_Nck2; Src homology 2 (SH2) domain found in Nck

cd11899
Location:2 → 59

SH3_Nck2_1; First Src Homology 3 domain of Nck2 adaptor protein

cd11902
Location:114 → 168

SH3_Nck2_2; Second Src Homology 3 domain of Nck2 adaptor protein

cd11903
Location:198 → 256

SH3_Nck2_3; Third Src Homology 3 domain of Nck2 adaptor protein