U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

prp-8 Pre-mRNA-splicing factor 8 homolog [ Caenorhabditis elegans ]

Gene ID: 176153, updated on 5-May-2024

Summary

Go to the top of the page Help
Gene symbol
prp-8
Gene description
Pre-mRNA-splicing factor 8 homolog
Primary source
WormBase:WBGene00004187
Locus tag
CELE_C50C3.6
See related
AllianceGenome:WB:WBGene00004187
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Caenorhabditis elegans (strain: Bristol N2)
Lineage
Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae; Caenorhabditis
Summary
Predicted to enable pre-mRNA intronic binding activity and snRNA binding activity. Predicted to be involved in spliceosomal tri-snRNP complex assembly. Predicted to be located in nucleus. Predicted to be part of U5 snRNP and catalytic step 2 spliceosome. Is expressed in germ line; intestine; and pharynx. Used to study retinitis pigmentosa. Human ortholog(s) of this gene implicated in retinitis pigmentosa and retinitis pigmentosa 13. Orthologous to human PRPF8 (pre-mRNA processing factor 8). [provided by Alliance of Genome Resources, Apr 2022]
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
Location:
chromosome: III
Exon count:
10
Sequence:
Chromosome: III; NC_003281.10 (8164373..8172710)

Chromosome III - NC_003281.10Genomic Context describing neighboring genes Neighboring gene Inositol polyphosphate 5-phosphatase Neighboring gene BTB and MATH domain-containing protein 42 Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene ncRNA Neighboring gene Uncharacterized protein Neighboring gene ncRNA Neighboring gene ncRNA

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_003281.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Crystal structure of the C-terminal domain of splicing factor Prp8 carrying retinitis pigmentosa mutants. Zhang L, et al. Protein Sci, 2007 Jun. PMID 17473007, Free PMC Article
  2. A forward genetic screen in C. elegans identifies conserved residues of spliceosomal proteins PRP8 and SNRNP200/BRR2 with a role in maintaining 5' splice site identity. Cartwright-Acar CH, et al. Nucleic Acids Res, 2022 Nov 11. PMID 36321655, Free PMC Article
  3. Suppressors of the cdc-25.1(gf)-associated intestinal hyperplasia reveal important maternal roles for prp-8 and a subset of splicing factors in C. elegans. Hebeisen M, et al. RNA, 2008 Dec. PMID 18945809, Free PMC Article
  4. RSR-2, the Caenorhabditis elegans ortholog of human spliceosomal component SRm300/SRRM2, regulates development by influencing the transcriptional machinery. Fontrodona L, et al. PLoS Genet, 2013 Jun. PMID 23754964, Free PMC Article
  5. β-Integrin de-phosphorylation by the Density-Enhanced Phosphatase DEP-1 attenuates EGFR signaling in C. elegans. Walser M, et al. PLoS Genet, 2017 Jan. PMID 28135265, Free PMC Article

See all (14) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. A forward genetic screen in C. elegans identifies conserved residues of spliceosomal proteins PRP8 and SNRNP200/BRR2 with a role in maintaining 5' splice site identity.
    Title: A forward genetic screen in C. elegans identifies conserved residues of spliceosomal proteins PRP8 and SNRNP200/BRR2 with a role in maintaining 5' splice site identity.
Submit: New GeneRIF Correction

Pathways from PubChem

Go to the top of the page

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Markers

Homology

Gene Ontology Provided by WormBase

Function Evidence Code Pubs
enables RNA binding IEA
Inferred from Electronic Annotation
more info
  
enables U1 snRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables U2 snRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables U5 snRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables U5 snRNA binding IEA
Inferred from Electronic Annotation
more info
  
enables U6 snRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables U6 snRNA binding IEA
Inferred from Electronic Annotation
more info
  
enables metallopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables peptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables pre-mRNA intronic binding IBA
Inferred from Biological aspect of Ancestor
more info
  
Process Evidence Code Pubs
involved_in RNA splicing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in mRNA splicing, via spliceosome IEA
Inferred from Electronic Annotation
more info
  
involved_in spliceosomal tri-snRNP complex assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
part_of U5 snRNP IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of catalytic step 2 spliceosome IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of ribonucleoprotein complex IEA
Inferred from Electronic Annotation
more info
  
part_of spliceosomal complex IEA
Inferred from Electronic Annotation
more info
  

General protein information

Go to the top of the page Help
Preferred Names
Pre-mRNA-splicing factor 8 homolog
NP_498785.1
  • Confirmed by transcript evidence

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003281.10 Reference assembly

    Range
    8164373..8172710
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_066384.7 → NP_498785.1  Pre-mRNA-splicing factor 8 homolog [Caenorhabditis elegans]

    See identical proteins and their annotated locations for NP_498785.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    P34369
    Conserved Domains (8) summary
    cd08056
    Location:2060 → 2310
    MPN_PRP8; Mpr1p, Pad1p N-terminal (MPN) domains without isopeptidase activity found in splicing factor Prp8
    COG5178
    Location:5 → 2329
    PRP8; U5 snRNP spliceosome subunit [RNA processing and modification]
    cd13838
    Location:1759 → 2009
    RNase_H_like_Prp8_IV; Ribonuclease-like Prp8 domain IV core
    pfam08082
    Location:48 → 198
    PRO8NT; PRO8NT (NUC069), PrP8 N-terminal domain
    pfam08083
    Location:388 → 788
    PROCN; PROCN (NUC071) domain
    pfam10596
    Location:1435 → 1593
    U6-snRNA_bdg; U6-snRNA interacting domain of PrP8
    pfam10597
    Location:1202 → 1334
    U5_2-snRNA_bdg; U5-snRNA binding site 2 of PrP8
    pfam10598
    Location:978 → 1069
    RRM_4; RNA recognition motif of the spliceosomal PrP8
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P34369.1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Chemical Information
Molecular Biology Databases
Research Materials
Tools
Miscellaneous