Gclm glutamate-cysteine ligase, modifier subunit [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Gclmprovided by MGI
Official Full Name: glutamate-cysteine ligase, modifier subunitprovided by MGI
Primary source: MGI:MGI:104995
See related: Ensembl:ENSMUSG00000028124 AllianceGenome:MGI:104995
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Gcmc; Glclr
Summary: Enables glutamate-cysteine ligase catalytic subunit binding activity. Acts upstream of or within several processes, including cellular response to leukemia inhibitory factor; glutathione biosynthetic process; and positive regulation of glutamate-cysteine ligase activity. Part of glutamate-cysteine ligase complex. Is expressed in several structures, including alimentary system; liver; nervous system; urinary system; and vibrissa. Human ortholog(s) of this gene implicated in myocardial infarction and vascular dementia. Orthologous to human GCLM (glutamate-cysteine ligase modifier subunit). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Ubiquitous expression in duodenum adult (RPKM 93.5), liver E14 (RPKM 50.1) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 3 G1; 3 52.94 cM

Exon count:: 7

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	3	NC_000069.7 (122039206..122060864)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	3	NC_000069.6 (122245487..122267215)

Chromosome 3 - NC_000069.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Alterations in TRN-anterodorsal thalamocortical circuits affect sleep architecture and homeostatic processes in oxidative stress vulnerable Gclm[-/-] mice.
Title: Alterations in TRN-anterodorsal thalamocortical circuits affect sleep architecture and homeostatic processes in oxidative stress vulnerable Gclm(-/-) mice.
The development of glutamate cysteine ligase [GCL] transgenic and gene deletion mouse lines are described.
Title: Glutamate cysteine ligase (GCL) transgenic and gene-targeted mice for controlling glutathione synthesis.
glutathione levels in the skin of Gclm knock-out mice are reduced by 70 %, which is sufficient for wound repair in young mice, but become rate-limiting upon aging
Title: Low levels of glutathione are sufficient for survival of keratinocytes after UV irradiation and for healing of mouse skin wounds.
Gclm(-/-) mice have accelerated ovarian aging due to ovarian oxidative stress.
Title: Glutamate Cysteine Ligase Modifier Subunit (Gclm) Null Mice Have Increased Ovarian Oxidative Stress and Accelerated Age-Related Ovarian Failure.
Upregulation of both GCLC and GLCM mRNA levels in response to cysteine deprivation was dependent on new protein synthesis.
Title: Upregulation of capacity for glutathione synthesis in response to amino acid deprivation: regulation of glutamate-cysteine ligase subunits.
In Gclm KO mice, early-life insults inducing oxidative stress are detrimental to immature parvalbumin interneurons and have long-term consequences.
Title: Early-life insults impair parvalbumin interneurons via oxidative stress: reversal by N-acetylcysteine.
Wild type female mice deficient in GSH due to deletion of Gclm were resistant to increase visceral adiposity and hepatic steatosis caused by exposure to the PAH BaP.
Title: In utero exposure to benzo[a]pyrene increases adiposity and causes hepatic steatosis in female mice, and glutathione deficiency is protective.
The glutathione synthesis gene Gclm modulates amphiphilic polymer-coated CdSe/ZnS quantum dot-induced lung inflammation in mice.
Title: The glutathione synthesis gene Gclm modulates amphiphilic polymer-coated CdSe/ZnS quantum dot-induced lung inflammation in mice.
Anxiety and drug-stimulated locomotor control brain regions are vulnerable to gclm/glutathione deficiencies.
Title: Effect of chronic glutathione deficiency on the behavioral phenotype of Gclm-/- knockout mice.
Clinically relevant levels of TGF-beta1 suppresses GCLC and GCLM expression in mouse lung.
Title: Transforming growth factor β suppresses glutamate-cysteine ligase gene expression and induces oxidative stress in a lung fibrosis model.

Submit: New GeneRIF Correction See all GeneRIFs (21)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (2) 1 citation
Endonuclease-mediated (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Gclm (e-PCR), detects polymorphism
- UniSTS:525987

D1S3412 (e-PCR)
- UniSTS:28861

Gclm (e-PCR), detects polymorphism
- UniSTS:527357

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables enzyme regulator activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to glutamate-cysteine ligase activity	ISO Inferred from Sequence Orthology more info
enables glutamate-cysteine ligase activity	ISO Inferred from Sequence Orthology more info
enables glutamate-cysteine ligase catalytic subunit binding	IDA Inferred from Direct Assay more info	PubMed
enables glutamate-cysteine ligase catalytic subunit binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within apoptotic mitochondrial changes	IGI Inferred from Genetic Interaction more info	PubMed
involved_in blood vessel diameter maintenance	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to leukemia inhibitory factor	IEP Inferred from Expression Pattern more info	PubMed
acts_upstream_of_or_within cysteine metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glutamate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in glutathione biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within glutathione biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in glutathione biosynthetic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within glutathione metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of extrinsic apoptotic signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glutamate-cysteine ligase activity	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of glutamate-cysteine ligase activity	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within regulation of mitochondrial depolarization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to nitrosative stress	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to oxidative stress	ISO Inferred from Sequence Orthology more info
involved_in response to xenobiotic stimulus	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
part_of glutamate-cysteine ligase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of glutamate-cysteine ligase complex	IDA Inferred from Direct Assay more info	PubMed
part_of glutamate-cysteine ligase complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: glutamate--cysteine ligase regulatory subunit

Names: GCS light chain; gamma-ECS regulatory subunit; gamma-glutamylcysteine synthetase regulatory subunit

NP_032155.1

EC 6.3.2.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.