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Pcyt1a phosphate cytidylyltransferase 1A, choline [ Rattus norvegicus (Norway rat) ]

Gene ID: 140544, updated on 5-Mar-2024

Summary

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Official Symbol
Pcyt1aprovided by RGD
Official Full Name
phosphate cytidylyltransferase 1A, cholineprovided by RGD
Primary source
RGD:70515
See related
Ensembl:ENSRNOG00000001762 AllianceGenome:RGD:70515
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
CTP; CCT-alpha
Summary
Enables several functions, including calmodulin binding activity; choline-phosphate cytidylyltransferase activity; and phosphatidylcholine binding activity. Involved in CDP-choline pathway. Located in endoplasmic reticulum; nuclear envelope; and plasma membrane. Human ortholog(s) of this gene implicated in spondylometaphyseal dysplasia with cone-rod dystrophy. Orthologous to human PCYT1A (phosphate cytidylyltransferase 1A, choline). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Heart (RPKM 257.0), Muscle (RPKM 202.1) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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Location:
11q22
Exon count:
10
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 11 NC_086029.1 (81818914..81862623, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 11 NC_051346.1 (68313860..68357828, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 11 NC_005110.4 (71547865..71592037, complement)

Chromosome 11 - NC_086029.1Genomic Context describing neighboring genes Neighboring gene zinc finger DHHC-type palmitoyltransferase 19 Neighboring gene solute carrier family 51 member A Neighboring gene uncharacterized LOC120095669 Neighboring gene uncharacterized LOC134481088 Neighboring gene dynein light chain Tctex-type 2B Neighboring gene transmembrane 4 L six family member 19

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Participation of prostaglandin D2 in the mobilization of the nuclear-localized CTP:phosphocholine cytidylyltransferase alpha in renal epithelial cells. Favale NO, et al. Biochim Biophys Acta, 2016 Jun. PMID 27032756
  2. Structural basis for autoinhibition of CTP:phosphocholine cytidylyltransferase (CCT), the regulatory enzyme in phosphatidylcholine synthesis, by its membrane-binding amphipathic helix. Lee J, et al. J Biol Chem, 2014 Jan 17. PMID 24275660, Free PMC Article
  3. CTP:phosphocholine cytidylyltransferase α (CCTα) and lamins alter nuclear membrane structure without affecting phosphatidylcholine synthesis. Gehrig K, et al. Biochim Biophys Acta, 2011 Jun. PMID 21504799
  4. [Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants]. Guan CX, et al. Zhongguo Ying Yong Sheng Li Xue Za Zhi, 2003 Feb. PMID 21207859
  5. Oxysterol activation of phosphatidylcholine synthesis involves CTP:phosphocholine cytidylyltransferase alpha translocation to the nuclear envelope. Gehrig K, et al. Biochem J, 2009 Feb 15. PMID 18980580

See all (29) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data show that exogenous prostaglandin D2 (PGD2) induces CTP:phosphocholine cytidylyltransferase alpha (CCTalpha) distribution to nuclear envelope from intranuclear Lamin A/C foci.
    Title: Participation of prostaglandin D2 in the mobilization of the nuclear-localized CTP:phosphocholine cytidylyltransferase alpha in renal epithelial cells.
  2. This work deciphers how the CCT regulatory amphipathic helix functions as a silencing device.
    Title: Structural basis for autoinhibition of CTP:phosphocholine cytidylyltransferase (CCT), the regulatory enzyme in phosphatidylcholine synthesis, by its membrane-binding amphipathic helix.
  3. ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase alpha contributes to malignant transformation of intestinal epithelial cells
    Title: ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.
  4. the 22-mer segment in the structurally pliable regulatory domain of rat CTP: phosphocholine cytidylyltransferase facilitates both silencing and activating functions
    Title: A 22-mer segment in the structurally pliable regulatory domain of metazoan CTP: phosphocholine cytidylyltransferase facilitates both silencing and activating functions.
  5. 7beta-estradiol can increase CTP: phosphorylcholine cytidylyltransferase activity in cultured lung explants.
    Title: [Effect of 17beta-estradiol on phosphorylcholine cytidylyltransferase activity from cultured rat lung explants].
  6. 127 fibroblasts was heterogeneous, with evidence of nucleoplasmic, cytoplasmic and NE staining.
    Title: CTP:phosphocholine cytidylyltransferase α (CCTα) and lamins alter nuclear membrane structure without affecting phosphatidylcholine synthesis.
  7. The 2.2-A structure reveals a CCT236 homodimer in complex with the reaction product, CDP-choline.
    Title: Crystal structure of a mammalian CTP: phosphocholine cytidylyltransferase catalytic domain reveals novel active site residues within a highly conserved nucleotidyltransferase fold.
  8. translocation and activation of CCTalpha at nuclear membranes by side-chain hydroxylated sterols are regulated by the cholesterol status of the cell
    Title: Oxysterol activation of phosphatidylcholine synthesis involves CTP:phosphocholine cytidylyltransferase alpha translocation to the nuclear envelope.
  9. Data show that anionic lipids, working via surface-localized pH effects, can promote membrane binding by modifying protein charge and hydrophobicity, which contributes to the membrane selectivity of CTP:phosphocholine cytidylyltransferase in vivo.
    Title: Both acidic and basic amino acids in an amphitropic enzyme, CTP:phosphocholine cytidylyltransferase, dictate its selectivity for anionic membranes.
  10. Membrane binding modulates the quaternary structure of CTP:phosphocholine cytidylyltransferase
    Title: Membrane binding modulates the quaternary structure of CTP:phosphocholine cytidylyltransferase.
Submit: New GeneRIF Correction See all GeneRIFs (13)

Pathways from PubChem

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General gene information

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Markers

Homology

Clone Names

  • MGC93248

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables calmodulin binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables choline-phosphate cytidylyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables choline-phosphate cytidylyltransferase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables choline-phosphate cytidylyltransferase activity ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables lipid binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables molecular function inhibitor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables phosphatidylcholine binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables phosphatidylcholine binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein homodimerization activity IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in CDP-choline pathway IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in CDP-choline pathway ISO
Inferred from Sequence Orthology
more info
  
involved_in phosphatidylcholine biosynthetic process IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in phosphatidylcholine biosynthetic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within phosphatidylcholine biosynthetic process ISO
Inferred from Sequence Orthology
more info
  
involved_in phosphatidylcholine biosynthetic process ISO
Inferred from Sequence Orthology
more info
  
involved_in phosphatidylcholine biosynthetic process TAS
Traceable Author Statement
more info
 PubMed 
Component Evidence Code Pubs
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
is_active_in endoplasmic reticulum IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in endoplasmic reticulum ISO
Inferred from Sequence Orthology
more info
  
located_in endoplasmic reticulum membrane ISO
Inferred from Sequence Orthology
more info
  
located_in extrinsic component of membrane NAS
Non-traceable Author Statement
more info
 PubMed 
located_in glycogen granule ISO
Inferred from Sequence Orthology
more info
  
located_in nuclear envelope IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
choline-phosphate cytidylyltransferase A
Names
CCT A
CT A
CTP:phosphocholine cytidylyl transferase
CTP:phosphocholine cytidylyltransferase A
CTP:phosphocholine cytidylyltransferase alpha
choline phosphate cytidylyltransferase 1 alpha
choline-phosphate cytidylyltransferase A-like
phosphate cytidylyltransferase 1, choline, alpha
phosphorylcholine transferase A
NP_511177.2
XP_006248506.1
XP_008766916.1
XP_038943847.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_078622.2 → NP_511177.2  choline-phosphate cytidylyltransferase A

    See identical proteins and their annotated locations for NP_511177.2

    Status: VALIDATED

    Source sequence(s)
    AW919077, BF285251, BQ780519, L13245
    UniProtKB/Swiss-Prot
    P19836
    UniProtKB/TrEMBL
    A6IRR3, A6IRR5
    Related
    ENSRNOP00000002403.3, ENSRNOT00000002403.4
    Conserved Domains (1) summary
    cd02174
    Location:75 → 224
    CCT; CTP:phosphocholine cytidylyltransferase

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086029.1 Reference GRCr8

    Range
    81818914..81862623 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_008768694.4 → XP_008766916.1  choline-phosphate cytidylyltransferase A isoform X1

    See identical proteins and their annotated locations for XP_008766916.1

    UniProtKB/Swiss-Prot
    P19836
    UniProtKB/TrEMBL
    A6IRR3, A6IRR5
    Conserved Domains (1) summary
    cd02174
    Location:75 → 224
    CCT; CTP:phosphocholine cytidylyltransferase

  2. XM_006248444.5 → XP_006248506.1  choline-phosphate cytidylyltransferase A isoform X1

    See identical proteins and their annotated locations for XP_006248506.1

    UniProtKB/Swiss-Prot
    P19836
    UniProtKB/TrEMBL
    A6IRR3, A6IRR5
    Conserved Domains (1) summary
    cd02174
    Location:75 → 224
    CCT; CTP:phosphocholine cytidylyltransferase

  3. XM_039087919.2 → XP_038943847.1  choline-phosphate cytidylyltransferase A isoform X1

    UniProtKB/Swiss-Prot
    P19836
    UniProtKB/TrEMBL
    A6IRR3, A6IRR5
    Conserved Domains (1) summary
    cd02174
    Location:75 → 224
    CCT; CTP:phosphocholine cytidylyltransferase
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P19836.2 GenPept UniProtKB/Swiss-Prot:P19836

Gene LinkOut

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