Slc6a3 solute carrier family 6 (neurotransmitter transporter, dopamine), member 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Slc6a3provided by MGI
Official Full Name: solute carrier family 6 (neurotransmitter transporter, dopamine), member 3provided by MGI
Primary source: MGI:MGI:94862
See related: Ensembl:ENSMUSG00000021609 AllianceGenome:MGI:94862
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: DAT; Dat1
Summary: Enables several functions, including dopamine binding activity; dopamine:sodium symporter activity; and protein N-terminus binding activity. Involved in dopamine uptake; hyaloid vascular plexus regression; and neurotransmitter uptake. Acts upstream of or within several processes, including dopamine metabolic process; gland development; and prepulse inhibition. Located in axon and cell surface. Is expressed in genitourinary system; nervous system; spleen; and submandibular gland. Used to study Gilles de la Tourette syndrome; attention deficit hyperactivity disorder; and schizophrenia. Human ortholog(s) of this gene implicated in attention deficit hyperactivity disorder; nicotine dependence; and obesity. Orthologous to human SLC6A3 (solute carrier family 6 member 3). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Low expression observed in reference dataset See more
Orthologs: human all
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Genomic context

Location:: 13 C1; 13 40.1 cM

Exon count:: 16

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	13	NC_000079.7 (73684270..73726791)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	13	NC_000079.6 (73536128..73578672)

Chromosome 13 - NC_000079.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Leaky lessons learned: Efflux prone dopamine transporter variant reveals sex and circuit specific contributions of D2 receptor signalling to neuropsychiatric disease.
Title: Leaky lessons learned: Efflux prone dopamine transporter variant reveals sex and circuit specific contributions of D2 receptor signalling to neuropsychiatric disease.
Abused drug-induced intracranial self-stimulation is correlated with the alteration of dopamine transporter availability in the medial prefrontal cortex and nucleus accumbens of mice.
Title: Abused drug-induced intracranial self-stimulation is correlated with the alteration of dopamine transporter availability in the medial prefrontal cortex and nucleus accumbens of mice.
Sex-dependent alterations in behavior, drug responses and dopamine transporter expression in heterozygous DAT-Cre mice.
Title: Sex-dependent alterations in behavior, drug responses and dopamine transporter expression in heterozygous DAT-Cre mice.
Dopaminergic loss of cyclin-dependent kinase-like 5 recapitulates methylphenidate-remediable hyperlocomotion in mouse model of CDKL5 deficiency disorder.
Title: Dopaminergic loss of cyclin-dependent kinase-like 5 recapitulates methylphenidate-remediable hyperlocomotion in mouse model of CDKL5 deficiency disorder.
Dopamine and the phosphorylated dopamine transporter are increased in the diacylglycerol kinase eta-knockout mouse brain.
Title: Dopamine and the phosphorylated dopamine transporter are increased in the diacylglycerol kinase η-knockout mouse brain.
Dopamine transporter gene expression within the nucleus accumbens plays important role in the acquisition and reinstatement of ethanol-seeking behavior in mice.
Title: Dopamine transporter gene expression within the nucleus accumbens plays important role in the acquisition and reinstatement of ethanol-seeking behavior in mice.
Enhanced Dopamine Transmission and Hyperactivity in the Dopamine Transporter Heterozygous Mice Lacking the D3 Dopamine Receptor.
Title: Enhanced Dopamine Transmission and Hyperactivity in the Dopamine Transporter Heterozygous Mice Lacking the D3 Dopamine Receptor.
Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action and in vivo impact.
Title: Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action and in vivo impact.
Autism-linked dopamine transporter mutation alters striatal dopamine neurotransmission and dopamine-dependent behaviors.
Title: Autism-linked dopamine transporter mutation alters striatal dopamine neurotransmission and dopamine-dependent behaviors.
The authors identify the dopamine transporter as a master regulator of dopamine short-term plasticity, governing the balance between release-dependent and independent mechanisms that also show region-specific gating.
Title: Plasticity in striatal dopamine release is governed by release-independent depression and the dopamine transporter.

Submit: New GeneRIF Correction See all GeneRIFs (80)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Targeted (24) 1 citation
Endonuclease-mediated (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Slc6a3 (e-PCR), detects polymorphism
- UniSTS:507231

PMC304098P2 (e-PCR)
- UniSTS:272521

D1Wox51 (e-PCR)
- UniSTS:239558

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables amine binding	ISO Inferred from Sequence Orthology more info
enables dopamine binding	IDA Inferred from Direct Assay more info	PubMed
enables dopamine:sodium symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables dopamine:sodium symporter activity	IDA Inferred from Direct Assay more info	PubMed
enables dopamine:sodium symporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables dopamine:sodium symporter activity	ISO Inferred from Sequence Orthology more info
enables heterocyclic compound binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables monoamine transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables monoamine transmembrane transporter activity	ISO Inferred from Sequence Orthology more info	PubMed
enables neurotransmitter transmembrane transporter activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables norepinephrine:sodium symporter activity	IBA Inferred from Biological aspect of Ancestor more info
enables norepinephrine:sodium symporter activity	ISO Inferred from Sequence Orthology more info
enables protease binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein phosphatase 2A binding	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	ISO Inferred from Sequence Orthology more info
enables signaling receptor binding	ISO Inferred from Sequence Orthology more info
enables symporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within adenohypophysis development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in amino acid transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within cognition	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within dopamine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within dopamine catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within dopamine transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dopamine transport	ISO Inferred from Sequence Orthology more info
involved_in dopamine uptake	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dopamine uptake	ISO Inferred from Sequence Orthology more info
involved_in dopamine uptake involved in synaptic transmission	IBA Inferred from Biological aspect of Ancestor more info
involved_in hyaloid vascular plexus regression	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within lactation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within locomotory behavior	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within locomotory behavior	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within monoamine transport	ISO Inferred from Sequence Orthology more info	PubMed
involved_in neurotransmitter transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neurotransmitter uptake	IDA Inferred from Direct Assay more info	PubMed
involved_in neurotransmitter uptake	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in norepinephrine transport	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of multicellular organism growth	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within prepulse inhibition	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within regulation of dopamine metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to cAMP	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to cocaine	IDA Inferred from Direct Assay more info	PubMed
involved_in response to ethanol	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within sensory perception of smell	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sodium ion transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in axon	IBA Inferred from Biological aspect of Ancestor more info
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in axon	ISO Inferred from Sequence Orthology more info
located_in axon terminus	IDA Inferred from Direct Assay more info	PubMed
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in cell surface	IDA Inferred from Direct Assay more info	PubMed
located_in cell surface	ISO Inferred from Sequence Orthology more info
is_active_in dopaminergic synapse	ISO Inferred from Sequence Orthology more info
part_of flotillin complex	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane raft	ISO Inferred from Sequence Orthology more info
located_in neuron projection	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	ISO Inferred from Sequence Orthology more info
is_active_in neuronal cell body membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in postsynaptic membrane	ISO Inferred from Sequence Orthology more info
is_active_in presynaptic membrane	IBA Inferred from Biological aspect of Ancestor more info
is_active_in presynaptic membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: sodium-dependent dopamine transporter

Names: DA transporter; dopamine transporter 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_010020.3 → NP_034150.1 sodium-dependent dopamine transporter

See identical proteins and their annotated locations for NP_034150.1

Status: VALIDATED

Source sequence(s)

AC154547, AC158359

Consensus CDS

CCDS26632.1

UniProtKB/Swiss-Prot

Q60719, Q61327, Q9R1I2

UniProtKB/TrEMBL

Q3UVW5, Q8C009, Q9JJ41, Q9R0X6

Related

ENSMUSP00000022100.7, ENSMUST00000022100.7

Conserved Domains (1) summary

cl00456
Location:60 → 613

SLC5-6-like_sbd; Solute carrier families 5 and 6-like; solute binding domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000079.7 Reference GRCm39 C57BL/6J

Range

73684270..73726791

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006517083.5 → XP_006517146.1 sodium-dependent dopamine transporter isoform X1

UniProtKB/TrEMBL

Q3UVW5, Q8C009, Q9JJ41, Q9R0X6

Conserved Domains (1) summary

cl00456
Location:60 → 569

SLC5-6-like_sbd; Solute carrier families 5 and 6-like; solute binding domain
XM_017315377.3 → XP_017170866.1 sodium-dependent dopamine transporter isoform X2

UniProtKB/TrEMBL

Q9JJ41

Conserved Domains (1) summary

cl00456
Location:60 → 533

SLC5-6-like_sbd; Solute carrier families 5 and 6-like; solute binding domain
XM_006517084.5 → XP_006517147.1 sodium-dependent dopamine transporter isoform X3

Conserved Domains (1) summary

cl00456
Location:60 → 385

SLC5-6-like_sbd; Solute carrier families 5 and 6-like; solute binding domain