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Clk2 CDC-like kinase 2 [ Mus musculus (house mouse) ]

Gene ID: 12748, updated on 21-Apr-2024

Summary

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Official Symbol
Clk2provided by MGI
Official Full Name
CDC-like kinase 2provided by MGI
Primary source
MGI:MGI:1098669
See related
Ensembl:ENSMUSG00000068917 AllianceGenome:MGI:1098669
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary
Enables protein serine/threonine kinase activity and protein tyrosine kinase activity. Involved in negative regulation of gluconeogenesis and protein autophosphorylation. Acts upstream of or within peptidyl-tyrosine phosphorylation and response to retinoic acid. Located in nucleus. Is expressed in several structures, including brain; genitourinary system; liver; retina; and thyroid gland. Orthologous to human CLK2 (CDC like kinase 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Ubiquitous expression in limb E14.5 (RPKM 37.3), CNS E11.5 (RPKM 31.9) and 28 other tissues See more
Orthologs
human all
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Genomic context

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Location:
3 F1; 3 39.01 cM
Exon count:
13
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 3 NC_000069.7 (89072102..89084394)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 3 NC_000069.6 (89164795..89177087)

Chromosome 3 - NC_000069.7Genomic Context describing neighboring genes Neighboring gene predicted gene, 29704 Neighboring gene STARR-seq mESC enhancer starr_08224 Neighboring gene pyruvate kinase liver and red blood cell Neighboring gene hyperpolarization-activated, cyclic nucleotide-gated K+ 3 Neighboring gene predicted gene, 45927 Neighboring gene STARR-positive B cell enhancer ABC_E2079 Neighboring gene secretory carrier membrane protein 3 Neighboring gene predicted gene 16069 Neighboring gene endosomal transmembrane epsin interactor 3 Neighboring gene CapStarr-seq enhancer MGSCv37_chr3:88994426-88994609 Neighboring gene STARR-positive B cell enhancer ABC_E275 Neighboring gene predicted gene, 23269

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. Exercise decreases CLK2 in the liver of obese mice and prevents hepatic fat accumulation. Muñoz VR, et al. J Cell Biochem, 2018 Jul. PMID 29575149
  2. Adipose Tissue CLK2 Promotes Energy Expenditure during High-Fat Diet Intermittent Fasting. Hatting M, et al. Cell Metab, 2017 Feb 7. PMID 28089567, Free PMC Article
  3. CLK2 inhibition ameliorates autistic features associated with SHANK3 deficiency. Bidinosti M, et al. Science, 2016 Mar 11. PMID 26847545
  4. Clk2 and B56β mediate insulin-regulated assembly of the PP2A phosphatase holoenzyme complex on Akt. Rodgers JT, et al. Mol Cell, 2011 Feb 18. PMID 21329884, Free PMC Article
  5. Stress-responsive maturation of Clk1/4 pre-mRNAs promotes phosphorylation of SR splicing factor. Ninomiya K, et al. J Cell Biol, 2011 Oct 3. PMID 21949414, Free PMC Article

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. CLK2 in GABAergic neurons is critical in regulating energy balance and anxiety-like behavior in a gender-specific fashion.
    Title: CLK2 in GABAergic neurons is critical in regulating energy balance and anxiety-like behavior in a gender-specific fashion.
  2. High CLK2 expression is associated with obesity.
    Title: Exercise decreases CLK2 in the liver of obese mice and prevents hepatic fat accumulation.
  3. CLK2 is a regulatory component of diet-induced thermogenesis in brown adipose tissue.
    Title: Adipose Tissue CLK2 Promotes Energy Expenditure during High-Fat Diet Intermittent Fasting.
  4. Liver-specific Clk2 knockout mice fed a high-fat diet exhibit increased fasting levels of blood ketone bodies, reduced respiratory exchange ratio, and increased gene expression of fatty acid oxidation and ketogenic pathways.
    Title: Cdc2-like kinase 2 suppresses hepatic fatty acid oxidation and ketogenesis through disruption of the PGC-1α and MED1 complex.
  5. LAMMER/Cdc2-like kinase, represented by Doa and its mammalian homolog CLK2, is a critical and conserved component in the regulatory program of the mitosis-to-meiosis switch
    Title: The highly conserved LAMMER/CLK2 protein kinases prevent germ cell overproliferation in Drosophila.
  6. Cdc2-like kinase 2 (Clk2) directly phosphorylates the SR domain on PGC-1alpha, resulting in repression of gluconeogenic gene expression and hepatic glucose output.
    Title: Cdc2-like kinase 2 is an insulin-regulated suppressor of hepatic gluconeogenesis.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Gene trapped (1) 
  • Targeted (1)  1 citation
  • Endonuclease-mediated (3) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Readthrough Gm45927

Readthrough gene: Gm45927, Included gene: Scamp3

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleotide binding IEA
Inferred from Electronic Annotation
more info
  
enables protein kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein serine/threonine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein serine/threonine kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein serine/threonine kinase activity ISO
Inferred from Sequence Orthology
more info
  
enables protein serine/threonine/tyrosine kinase activity IEA
Inferred from Electronic Annotation
more info
  
enables protein tyrosine kinase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables protein tyrosine kinase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in negative regulation of gluconeogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in peptidyl-tyrosine phosphorylation IBA
Inferred from Biological aspect of Ancestor
more info
  
acts_upstream_of_or_within peptidyl-tyrosine phosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within phosphorylation IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within protein autophosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein autophosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein phosphorylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein phosphorylation ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of RNA splicing IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of RNA splicing ISO
Inferred from Sequence Orthology
more info
  
involved_in response to ionizing radiation ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within response to organic substance IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within response to retinoic acid IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in nuclear body ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
dual specificity protein kinase CLK2
NP_001156904.1
NP_031738.2

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001163432.2 → NP_001156904.1  dual specificity protein kinase CLK2 isoform 2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.
    Source sequence(s)
    AC161600, BY021174, BY247622, BY738871, CD740936, CN538691, DT924403
    Consensus CDS
    CCDS50958.1
    UniProtKB/TrEMBL
    E9Q5Y1, F8WHX0
    Related
    ENSMUSP00000088445.6, ENSMUST00000090927.12
    Conserved Domains (2) summary
    smart00220
    Location:161 → 477
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd14215
    Location:148 → 477
    PKc_CLK2; Catalytic domain of the Dual-specificity protein kinase, CDC-like kinase 2

  2. NM_007712.4 → NP_031738.2  dual specificity protein kinase CLK2 isoform 1

    See identical proteins and their annotated locations for NP_031738.2

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
    Source sequence(s)
    AC161600, BY021174, BY247622, BY738871, CD740936, CN538691, DT924403
    Consensus CDS
    CCDS17491.1
    UniProtKB/Swiss-Prot
    E9Q721, O35491
    UniProtKB/TrEMBL
    E9Q5Y1
    Related
    ENSMUSP00000113390.3, ENSMUST00000121212.9
    Conserved Domains (2) summary
    smart00220
    Location:162 → 478
    S_TKc; Serine/Threonine protein kinases, catalytic domain
    cd14215
    Location:149 → 478
    PKc_CLK2; Catalytic domain of the Dual-specificity protein kinase, CDC-like kinase 2

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000069.7 Reference GRCm39 C57BL/6J

    Range
    89072102..89084394
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O35491.2 GenPept UniProtKB/Swiss-Prot:O35491

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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